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OfflineThe Tao
Look at the flowers.


Registered: 09/12/19
Posts: 1,550
Loc: Canada
Last seen: 1 hour, 26 minutes
Re: Official Copelandia/Panaeolus Thread [Re: Bismillah]
    #28537509 - 11/10/23 05:08 PM (2 months, 17 days ago)

Beautiful. I love cakes.


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Invisiblekirkeng
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Registered: 02/14/21
Posts: 805
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #28537653 - 11/10/23 06:49 PM (2 months, 17 days ago)

Quote:

Baba Yaga said:
Has anyone ever seen bisporus discolor? I have rust brown spots appear two weeks or so after full colonization. It's not the mycelium turning color but the verm/brf. Any clues?





I have seen this in older cultures on agar, I think I have a plate where it shows somewhere in my office, I’ll look tomorrow for it and report back if I find it. My last clone I ran showed discoloration in red dots, though I think the discoloration showed up later. I’m usually running cultures before they grow out much and I don’t recall ever seeing it until the plate is mostly grown out.

All that being said I can’t recall ever seeing it outside of an agar plate… I’ll also check my older tubs tomorrow to see if that rust has shown anywhere.


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InvisibleBaba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] * 1
    #28537682 - 11/10/23 07:07 PM (2 months, 17 days ago)

Thanks man, just had a look at the label on the pucks and it said inoclulated on Oct. 17 so this turned up about 18 days after full colonization. I remember seeing the rust color appear first right around the transferred agar piece. The agar plates from the same transfer session are looking all normal so far.

Your comment is reassuring as it doesn't look like any mold and bacteria wouldn't spread like that on BRF I suppose. Would like to see your plate....oh wait....I just remembered seeing a spot like this on one of the germination plates......Cool, I had it still sitting on the kitchen bench.


This one is 5 weeks old



Edited by Baba Yaga (11/10/23 07:45 PM)


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Invisiblekirkeng
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28537716 - 11/10/23 07:34 PM (2 months, 17 days ago)

Yea that’s exactly what I remember mine looking like! I imagine it’s gotta just be some age something or metabolite coloration?


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InvisibleSwabMarleyS
Twisted Metal Head
Male


Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] * 1
    #28538075 - 11/11/23 04:54 AM (2 months, 17 days ago)

I’ve had it in old agar plates. Like concentrated myc balls that kinda go brown. Like you say, maybe some sort of metabolite production. I think it’s just because the mycelium has aged maybe?


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 458
Loc: lives in trees
Last seen: 1 hour, 35 minutes
Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] * 2
    #28538195 - 11/11/23 07:16 AM (2 months, 17 days ago)

Just checking in with poo/millet experiments and MS bisporus.

Bisporus inoculated from agar to straight millet a week to ten days ago is super slow.  May as well be stalled though it is moving.  Shaking didn't seem to help encourage the mycelium to take off either.  I'll probably dump these grains and recycle them back into other projects.

Bisporus inoculated to millet soaked in compost tea as well as millet jars with a pinch of compost added directly to it were inoculated morning before last (or maybe night before last).  Bisporus is already crawling off the wedge and onto several nearby grains in both sets of jars.  Compost tea jars seem a little slower than compost added directly but I think I could dial in the compost tea to work a bit better (higher ratio of tea to water in my grain soak).  Also, it's still to early to tell really which are running faster.

But, both are running, which will give me four jars to play with here in a week or two.

Also, made some agar plates with millet water, compost tea, and 0.5% LME.  Put two drops of spores on eight plates yesterday.  Four of these and four 1% LME.  Going to rotate some of my current plates onto these hybrid plates before dropping more wedges on grains.  My thought is to train the mycelium on millet before putting it to grains.

I'll check back in with pictures once I have some worth sharing.

As an aside, my incubator maybe holding heat to well.  Checked on it this morning, after it had been running all night at 72.  Temperature read 84.  I assume it had been maintaining at 72 when the heat kicked on this morning in the house and continued to radiate heat from the heating pad and the perlite while the ambient temperature in the room rose.  During the day, when the heat in the house is on, it sits at 72 without issues.

Swapping lids to this one to hopefully disipate heat better.  Going to stuff with polyfill to keep the casings from drying out (made casing in the dehydrator in a bag and it was stupid easy for small scale casing - made a quart.  But with multiple bags and racks it would be easy to scale this up to 8 or more quarts and the temperature of the dehydrator makes it practically set and forget).  Still ATL 7 going in it.



The incubator with my cultures/grains was fine this morning, right in the 72-74 range when I checked it.  So it's working well and the condensation is minimal.



All that rye in those 3/4 quart jars is ATL 7 (either MS or clones).  The quart jars on the left marked PB are the Pans mentioned above.


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Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Edited by tree frog (11/11/23 07:27 AM)


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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 37 minutes
Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 3
    #28540177 - 11/12/23 12:02 PM (2 months, 16 days ago)

development

anyone wanna haphazard a guess? :tongue2:



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Willpower is the one true virtue



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OfflineGenome794
Spore-Whore-der & Agar-Addict


Registered: 07/20/22
Posts: 438
Loc: The Blue Planet
Last seen: 2 hours, 49 minutes
Re: Official Copelandia/Panaeolus Thread [Re: smalltalk_canceled] * 6
    #28540236 - 11/12/23 12:32 PM (2 months, 16 days ago)

Making progress...

Pan Jamaica finally germinated


And Some TTBVI doing well on white sorghum in two jars.



Looks nothing like the previou sculture I tried. I can at least tell for myself that they are good.


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InvisibleBaba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Genome794]
    #28540585 - 11/12/23 04:19 PM (2 months, 15 days ago)

:awedance::dancingshroom:


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OfflineShroomhunts
Hunter Gatherer
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Registered: 05/07/18
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 6
    #28540893 - 11/12/23 08:37 PM (2 months, 15 days ago)



First flush of Peter Pans


--------------------

You never kno


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OfflineSupaThaRipper
Genetics Hoarder
I'm a teapot User Gallery


Registered: 09/02/13
Posts: 1,504
Loc: USA
Last seen: 1 hour, 24 minutes
Re: Official Copelandia/Panaeolus Thread [Re: Shroomhunts]
    #28540896 - 11/12/23 08:40 PM (2 months, 15 days ago)

Quote:

Shroomhunts said:


First flush of Peter Pans




Lol Is that fucking water crystals or orbeez you stole from your kids?


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InvisibleMr Piggy
Big Dick Retard
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Registered: 09/29/11
Posts: 8,393
Re: Official Copelandia/Panaeolus Thread [Re: SupaThaRipper]
    #28540899 - 11/12/23 08:40 PM (2 months, 15 days ago)

I am very curious as to what that is.


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🅃🄴🄰🄼 🄵🄾🄸🄻


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OfflineSupaThaRipper
Genetics Hoarder
I'm a teapot User Gallery


Registered: 09/02/13
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Re: Official Copelandia/Panaeolus Thread [Re: Mr Piggy] * 4
    #28540905 - 11/12/23 08:42 PM (2 months, 15 days ago)

It’s nothing. He’s shitposting on every cultivation thread with AI


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OfflineShroomhunts
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Registered: 05/07/18
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Re: Official Copelandia/Panaeolus Thread [Re: Mr Piggy] * 1
    #28540907 - 11/12/23 08:44 PM (2 months, 15 days ago)


Okay I get it make fun of the mini monotub noob… check out my bucket tek tho pretty good huh?


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You never kno


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InvisibleMr Piggy
Big Dick Retard
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Registered: 09/29/11
Posts: 8,393
Re: Official Copelandia/Panaeolus Thread [Re: Shroomhunts] * 1
    #28540908 - 11/12/23 08:44 PM (2 months, 15 days ago)

Quote:

Shroomhunts said:


First flush of Peter Pans




As a matter of fact, a lot of the pics you are posting today look AI generated.  If so, your ratings are going to take a serious hit, people do not appreciate that kind of shenanigan.


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OfflineShroomhunts
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Re: Official Copelandia/Panaeolus Thread [Re: Mr Piggy]
    #28540912 - 11/12/23 08:45 PM (2 months, 15 days ago)

Ohh no, bad ratings! How will I afford my next Ferrari payment?


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You never kno


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InvisibleMr Piggy
Big Dick Retard
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Registered: 09/29/11
Posts: 8,393
Re: Official Copelandia/Panaeolus Thread [Re: Shroomhunts] * 3
    #28540913 - 11/12/23 08:47 PM (2 months, 15 days ago)

Whelp, I've reported you for spamming and am putting you on ignore.  Bye.


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🅃🄴🄰🄼 🄵🄾🄸🄻


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OfflineSupaThaRipper
Genetics Hoarder
I'm a teapot User Gallery


Registered: 09/02/13
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Loc: USA
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Re: Official Copelandia/Panaeolus Thread [Re: Shroomhunts]
    #28540916 - 11/12/23 08:47 PM (2 months, 15 days ago)

Bro that bucket tek is 🔥 shits all over damions


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 458
Loc: lives in trees
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Re: Official Copelandia/Panaeolus Thread [Re: SupaThaRipper] * 2
    #28541149 - 11/13/23 06:27 AM (2 months, 15 days ago)

So, this not being the AI picture thread aside...

Thanks to whoever a few pages back suggested sterilizing poo, grain, and  whatever else (I went with CV because I had some prepped) and inoculating the bag with an agar wedge.

Spotted some promising growth in one of the four bags when I checked them this morning.

Also got a couple LCs going that look pretty good so far.

The 0.5% LME +50/50 millet water and a few ml compost tea agar doesn't seem to be working out yet.  Maybe the myc needs a few days to adapt to it.  But the wedges I put on it haven't recovered while the stuff I put on 1% the same day is just smaller than a dime.  Could be faint growth too from low nutrient content.  I'll have to look a bit closer.  I put both an ATL 7 clone and my MS Bisporus culture on it and they both looked the same this morning.

The ATL 7 I'm running is pinning.  I'm hoping the fruiting chamber will work well for Pan. Bisporus too.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: Shroomhunts]
    #28541250 - 11/13/23 08:54 AM (2 months, 15 days ago)

Quote:

Shroomhunts said:

Okay I get it make fun of the mini monotub noob… check out my bucket tek tho pretty good huh?




You can stop now. Thanks. This is your warning. If you can't respect the community, you'll be booted, I'm sure you don't care, just entertaining due diligence, the ban hammer is just a nuke button away.


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