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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah] * 2
    #28510073 - 10/18/23 11:34 PM (3 months, 9 days ago)

The earliest I would have sent those out would be 2022 or 2023.


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What are they saying? Listen carefully, it might be something you need to hear...





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OfflineCrimsonRambeler
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #28510108 - 10/19/23 12:15 AM (3 months, 9 days ago)




Just a quick update...

I grew on barley not corn but I don't know when I was looking at them in person they looked messed up but in the pics there doesn't seem to be anything wrong that I can tell. This is a new contaminant that I'm encountering or Panaeolus cincticulus mycelium/sclerocia, what do all of you think? I'm glad I have another print from May just in case not going to Agar first was an irreversible mistake


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OfflineCrimsonRambeler
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Re: Official Copelandia/Panaeolus Thread [Re: CrimsonRambeler]
    #28510480 - 10/19/23 12:30 PM (3 months, 9 days ago)

So I apologize for not realizing that pictures aren't the best judge of health in regards to Panaeolus.(unless their pics of flushes!) But really I wanted to jump right into this because I got some super good knowledge from a former acquaintance and I have had a few things "click" off what Jo'c has said(Mycogeeky podcast) and have watched Mycophilia on YouTube(no patience for Patreon 'nless it's Gordo) & so against the essential "no" common sense to do spore to grain with a truffle producer & a Panaeolus respectively, I knowingly did it anyway. I do have one extra print that I wanted to go to a friend in Turkey, although I may have to use it or use part of it for myself to get the good genetic line going. I'm serious about this project and look forward to seeing its finality which will probably end with a hole bunch of rabbit holes; like Panaeolus cincticulus has grown to be the size of cubensis.


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Offlineodissey
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Re: Official Copelandia/Panaeolus Thread [Re: CrimsonRambeler]
    #28510602 - 10/19/23 01:53 PM (3 months, 9 days ago)

What is the dose conversion of wet to dry weight? Something like 12g wet = 1g dry?


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Invisiblekirkeng
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Re: Official Copelandia/Panaeolus Thread [Re: odissey] * 3
    #28510611 - 10/19/23 01:58 PM (3 months, 9 days ago)

Mine have pretty consistently been 93-96% water when wet, so drying down to 7-4% of wet weight. Pretty consistent across cyans, bisporus, and cambos.


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Offlinesmalltalk_canceled
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
    #28510759 - 10/19/23 04:06 PM (3 months, 9 days ago)



Too dry? Do i want beads/ pearls all the way here?


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: smalltalk_canceled] * 2
    #28511272 - 10/20/23 12:15 AM (3 months, 8 days ago)

Sorry bro but have you lost interest half way through the process? I'm confused....what is this :confused2:


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Offlineodissey
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
    #28511279 - 10/20/23 12:29 AM (3 months, 8 days ago)

Quote:

kirkeng said:
Mine have pretty consistently been 93-96% water when wet, so drying down to 7-4% of wet weight. Pretty consistent across cyans, bisporus, and cambos.



Thanks! So 14 ish grams wet should be nearly 1 g dry. But what about the actual effects? Isn't a fruit slightly more potent wet than the same fruit dried? I remember taking 12 g fresh of estero and being quite underwhelmed though, so maybe something like 20-22g fresh is a more adequate dose


Edited by odissey (10/20/23 12:54 AM)


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OnlineAspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: odissey]
    #28511396 - 10/20/23 06:38 AM (3 months, 8 days ago)

Quote:

odissey said:
Quote:

kirkeng said:
Mine have pretty consistently been 93-96% water when wet, so drying down to 7-4% of wet weight. Pretty consistent across cyans, bisporus, and cambos.



Thanks! So 14 ish grams wet should be nearly 1 g dry. But what about the actual effects? Isn't a fruit slightly more potent wet than the same fruit dried? I remember taking 12 g fresh of estero and being quite underwhelmed though, so maybe something like 20-22g fresh is a more adequate dose




I was psyched to try fresh fruits. Turned out they were indistinguishable from dried fruits.


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OfflineTelomere
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Re: Official Copelandia/Panaeolus Thread [Re: AspectOfTheCreator] * 4
    #28511503 - 10/20/23 08:55 AM (3 months, 8 days ago)

I thought this Pan Bispo spore germ plate was looking pretty sexy. Took three transfers a few days ago and they are all super fast moving.

Thanks @thirdeyewild



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Invisiblekirkeng
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Re: Official Copelandia/Panaeolus Thread [Re: AspectOfTheCreator]
    #28511563 - 10/20/23 10:09 AM (3 months, 8 days ago)

Quote:

odissey said:
Quote:

kirkeng said:
Mine have pretty consistently been 93-96% water when wet, so drying down to 7-4% of wet weight. Pretty consistent across cyans, bisporus, and cambos.



Thanks! So 14 ish grams wet should be nearly 1 g dry. But what about the actual effects? Isn't a fruit slightly more potent wet than the same fruit dried? I remember taking 12 g fresh of estero and being quite underwhelmed though, so maybe something like 20-22g fresh is a more adequate dose



5% of 20g would be 1g so that sounds a bit better. I personally don’t notice a huge difference in wet vs dry aside from a more pleasant eating experience. That beings said I don’t weigh things when I take them and opt to just take β€œwhatever feels right” so take my words with a grain of salt. I like an adequate 3 finger pinch.


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OfflineGorguss
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] * 2
    #28511587 - 10/20/23 10:34 AM (3 months, 8 days ago)

Eye balling pans

:salute:


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Offlinetree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere]
    #28511608 - 10/20/23 10:51 AM (3 months, 8 days ago)

Quote:

Telomere said:
I thought this Pan Bispo spore germ plate was looking pretty sexy. Took three transfers a few days ago and they are all super fast moving.

Thanks @thirdeyewild






I started four germ plates a week or so ago and just started four more this morning.

This is my first time with them so I appreciate the agar picture.

I have visible growth on one plate.  It looks a lot like what you posted but a lot smaller.  I haven't transferred it yet because it getminated towards the edge of the plate and I worry it's satellite mold because of the location.

If anyone has thoughts on what I should do.  Right now I'm leaning towards waiting for more germination so I have a better idea what I'm looking at.


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Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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OfflineWhiteDemon
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28511690 - 10/20/23 12:06 PM (3 months, 8 days ago)

Hello! It is okay to sterilize the casing instead of pasteurizing it? Can i just bucket tek it?


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OfflineTelomere
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #28511752 - 10/20/23 12:42 PM (3 months, 8 days ago)

Quote:

tree frog said:
Quote:

Telomere said:
I thought this Pan Bispo spore germ plate was looking pretty sexy. Took three transfers a few days ago and they are all super fast moving.

Thanks @thirdeyewild






I started four germ plates a week or so ago and just started four more this morning.

This is my first time with them so I appreciate the agar picture.

I have visible growth on one plate.  It looks a lot like what you posted but a lot smaller.  I haven't transferred it yet because it getminated towards the edge of the plate and I worry it's satellite mold because of the location.

If anyone has thoughts on what I should do.  Right now I'm leaning towards waiting for more germination so I have a better idea what I'm looking at.




The other couple pan cultures I’ve played with so far didn’t quite look this awesome and weren’t this fast. I’d give yours a while longer to see how it progresses.

Also, I recently changed to grain water agar (grain water diluted 50/50 with water) and I’ve found that everything I put on it seems to love it. Ymmv.


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Invisiblethirdeyewild
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere] * 1
    #28511754 - 10/20/23 12:45 PM (3 months, 8 days ago)

Those were easily the fastest moving species I have ever grown.


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Offlinetree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere]
    #28511871 - 10/20/23 02:04 PM (3 months, 8 days ago)

Thanks.  I'll give it a few days and see how it develops.

When people say grain water agar, is that from the soak or the boil?  I made some grain water lcs yesterday from my rye berry boil for sclerotia and cut it with three parts water.

I think my millet soak water might be okay without dilution since it's not getting boiled.  But maybe I should cut that 50/50?

Was planning to make some in the next few days.


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Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 3
    #28511982 - 10/20/23 03:13 PM (3 months, 8 days ago)

Bisporus germ plate on 1% LMEA.




So far I like how bisporus mycelium looks. Not so much fuzz.


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Offlinetree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28512010 - 10/20/23 03:34 PM (3 months, 8 days ago)

Here's the best photo without setting up the flowhood.  First noticed it day before yesterday.  Probably four times that size now.

On 2% LME.  Pan. Bisporus.  Nothing else noticable so far on any other plates.

I generally don't get satellites around my edges anymore but this looks like a place I cooled my inoculation loop before dropping spore solution on it.  Rather than a place I streaked.

But solution could have ran into the crack.

Anyway, possibly mold.

?


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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OnlineAspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #28513092 - 10/21/23 02:14 PM (3 months, 7 days ago)

Im planning on spawning to 25% pasteurized manure in coir. I can pasteurize the field capacity manure in jars at 150F for 1.5 hours, add extra water to it (enough to bring coir to field capacity), then dump the watery manure into the coir and mix. Correct?


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