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Baba Yaga
♥ coir grower

Registered: 09/13/20
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 2
#28506955 - 10/16/23 02:17 PM (3 months, 12 days ago) |
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Quote:
Bismillah said: I pulled two fruits, one for cloning and one for printing. I notice the fruits are not coming up in the center, and the one's along the wall get black. Any ideas what that could be?

The tub on the right is four days behind, and it has developed this thick mat in the middle. The humidity is 5-8 %points higher now compared to 4-5 days ago. (I made a wick humidifier on the inlet) Same spawn, bulk and casing. Is overlay caused by the environment, or could it be contamination?

I also have this pink spot that's growing. It's beneath the exhaust tube, and I know some condensation was dripping down there before.

What the nice folks above said, all this is contam related black caps are bacterial blotching (great observation spotting those milky droplets) trich and the yellow could be one of the aspergillus species which starts yellow and turns red. It doesn't spread like the other molds but it's not helping the grow. Keep it separate and you might get more fruits from this but I wouldn't take any spore prints from this tub and be extra thorough when cleaning up the clone and definitely make a comment in your notes about the mold.

Could be the sub being too wet or had wet spots at spawn or the other usual suspects like pasteurization flaw or the spawn itself.
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Gorguss
Chaotic sums


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28506960 - 10/16/23 02:22 PM (3 months, 12 days ago) |
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I'm trying to study the myc more. Got any material on how to use a microscope in mycology to inspect for contam?
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss] 1
#28506981 - 10/16/23 02:36 PM (3 months, 12 days ago) |
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I have seen a few posts with the same subject over the years and search engine is turning some up when keeping it to "main posts only". Like this
https://www.shroomery.org/forums/showflat.php/Number/24873961
Maybe ask in advanced mycology and see if someone like Alan comments.
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Bismillah
Scharf im Schlafanzug


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28507024 - 10/16/23 03:14 PM (3 months, 11 days ago) |
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Alright, so the trich one I get rid of asap. These bacterial fruits, are they safe to eat? Make tea perhaps?
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CrimsonRambeler
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss]
#28507097 - 10/16/23 04:14 PM (3 months, 11 days ago) |
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Basically if the Hypha is dark that can possibly be a good thing cause it's the mycelium you are trying to grow but if it's clear and wispy it is likely you have a non-basidiomycotoa fungal hitch hiker.
Edited by CrimsonRambeler (10/16/23 04:15 PM)
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kirkeng
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Pardon my shitty picture but have gotten a, in my opinion, rather cool situation. A calico spored pan.


Primarily red spores with the slight black streaks, kinda hard to tell in the first picture as I did not capture it well but the black streaks can be seen within the red gills as well!
This arose from an attempted cross of Australian RDU and weza pan cyans. Not claiming anything at this point but this mushroom arose from the third flush of the F1 run. Only a single mushroom in the whole tub appeared like this. Previous flushes lended black prints with a very slight red aura around them of which I could not capture well on camera. This print was exceptionally distinct in the two differing colors however still hard to capture as the print is from an exceptionally small cap.
Edited by kirkeng (10/16/23 08:00 PM)
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] 1
#28507488 - 10/16/23 08:48 PM (3 months, 11 days ago) |
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Very nice
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CrimsonRambeler
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] 1
#28507623 - 10/17/23 12:34 AM (3 months, 11 days ago) |
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That's pretty cool man, any other phenos in the F1 that stuck out as being a mix between the two? I wonder if the F2 if fruited from red-purple/black will produce anything crazy like the F2 flushes from Cubes that tend to show up around the F2. Either way good luck and I hope you can get both red and black spores from the same mushroom! That'd be wild!
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PsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] 1
#28507784 - 10/17/23 07:01 AM (3 months, 11 days ago) |
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Very Cool Kirkeng Did I send you those RDU genetics? I never saw a calico one (I didn’t try any crosses either), but I did notice a fair bit of variation is spore print color. Some almost traditional black and some strikingly red.
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Kunta Kente
BroBot 9k



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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 4
#28507812 - 10/17/23 07:50 AM (3 months, 11 days ago) |
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First pans, first MSS. Only a few poking out after the first pin showed 2-3 days ago, but I consider this a good success as I'm still misting them and no aborts. Goliath. Hopefully my other tubs from a similar delayed run start popping off, or my bisporus.
Still, I'm happy to have these guys to print / clone.
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Everything I have typed is purely fiction, and is purely for personal amusement. All images I've posted are not mine and I am simply posting interesting things I found on the Google.
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kirkeng
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 1
#28507869 - 10/17/23 09:10 AM (3 months, 11 days ago) |
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Quote:
CrimsonRambeler said: That's pretty cool man, any other phenos in the F1 that stuck out as being a mix between the two? I wonder if the F2 if fruited from red-purple/black will produce anything crazy like the F2 flushes from Cubes that tend to show up around the F2. Either way good luck and I hope you can get both red and black spores from the same mushroom! That'd be wild!
That’s partially what I’m shooting for is the combo red and black spores. I’m not confident things took just yet and remaining skeptical until I can grow out some f2s. That being said there was some abnormalities mainly in bruising patterns and uv flourescence patterns that suggests something funny. I have a more detailed write up in my journal here. https://www.shroomery.org/forums/showflat.php/Number/28448073
Quote:
PsiloPsychIn said: Very Cool Kirkeng Did I send you those RDU genetics? I never saw a calico one (I didn’t try any crosses either), but I did notice a fair bit of variation is spore print color. Some almost traditional black and some strikingly red.
Thanks psilo! No I got these from sporeworks *I think* a while back, the RDU arose from black prints on my first successful grow of pans. Good to know about variation, that could be an explanation of the weirdness. I’ve ran these guys a fair bit and they’ve remained consistent in bright red-burgundy color. I’m hoping this “calico” can get more prominent and persist even the gills present that calico appearance. Either way whether things are successful or not still a fun project to work on this winter. Got a clone of that Komodo from you going down soon though!
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PsiloPsychIn
PsiloPsychIn



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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] 5
#28507924 - 10/17/23 10:03 AM (3 months, 11 days ago) |
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I sent out quite a few Panaeolus Cambodginiensis var Komodo Island prints out over the past 4-5 months…
I might have missed it because I don’t get on here every day, but I have not seen any “Komodo Grows” is anyone having any success with the prints I sent out?
I would really like to see some of those really cool looking “Komodo Kaps” in here. I think there is a lot of potential in those genetics, in part because they are only a few generations from the sample that was collected in the wild. In other words, there are still some pretty wild genetics in those prints. Good luck to any of you working with them!



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PsiloPsychIn
PsiloPsychIn



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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] 1
#28507929 - 10/17/23 10:08 AM (3 months, 11 days ago) |
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Whoa! Am I reading that correctly? Are you the person who saw the RDU mutation emerge from a standard Pan Cyan Australian grow?
I saved one print from my RDU grows that is a really beautiful pure red color. This is all reminding me I need to dust off that print and try another RDU grow. I recall some of them produced quite large fruits.
Keep up the good work - looking forward to seeing what you do with the Komodo! 😀
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kirkeng
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 1
#28508015 - 10/17/23 11:21 AM (3 months, 11 days ago) |
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No I do not believe I am the one to first see it. I do not however recall receiving a specific RDU print and rather just an Australian print which was black when received, very well could have been an RDU print that exhibited very dark near black spores as you mention seeing before, that initial grow lended many black and red spores fruits. This was maybe 3 years ago? But I believe workman had RDU well before that with Australian. But I’m not sure of the history. He is the one who inadvertently inspired me to try the cross from some conversations. So I won’t make any claim to what you’re saying, folks much more knowledgeable and skilled than I deserve that, haha. The RDU I have now is very stable though and since growing it from that initial batch I’ve not seen any deviation from good distinct red spore.
I have had some success with the Komodo, maybe 3 tubs but was fruiting them in the summer heat and didn’t bother with pictures, but have higher hopes for the clone. But man I liked eating them, they have been fiesty by my experience. I have not seen the nice cap pheno though, perhaps someday it’ll arise.
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soppos


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Bismillah
Scharf im Schlafanzug


Registered: 07/11/22
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Re: Official Copelandia/Panaeolus Thread [Re: soppos]
#28509225 - 10/18/23 09:44 AM (3 months, 10 days ago) |
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My germ plates appear to be stalling. I've read before that pans like grain water, but others here say they use MEA with good results. The only other variables I can think of are temperature, light, age (of agar) and gas exchange. My plates are in a constant 20C environment, indirect light from a window, and I wrap them pretty tight 2-3 times around with saran wrap. These are vented plates. How do you guys wrap and store your plates?
My first suspicion is age. I brewed the agar 2 months ago, and they've been stored in 15C in a stack with the plastic sleeve on. My first pan culture was very happy with this mix of grain/potato water. This is the current state of affairs:
 3 weeks since germination.
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SwabMarley
Twisted Metal Head



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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah] 1
#28509321 - 10/18/23 11:07 AM (3 months, 10 days ago) |
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Pans are quite happy on standard LME plates in my experience. All the bells and whistles aren’t necessary. Just treat them exactly as you would cubes.
My first question would be; how old is the Pan print/swab you are trying to germinate?
The fuck is going on in those pictures!? If you are looking for a clean germ plate I would forget all the dyes etc. just a simple ass MEA plate is all that’s needed.
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Bismillah
Scharf im Schlafanzug


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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley]
#28509446 - 10/18/23 12:32 PM (3 months, 10 days ago) |
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Alright, will do. Yeah this food color didn't work out too well. I just wanted to differentiate gwa from mea. It's weird though, even though growth has slowed or stalled the mycelium is still eating the dye.
Edit: alcohol obscured the date on this print. It's 2020 something, cyan cambo komodo from psilopsychin.
Edited by Bismillah (10/18/23 12:56 PM)
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SwabMarley
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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah] 1
#28509494 - 10/18/23 01:12 PM (3 months, 10 days ago) |
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I just germed the very same print so will let you know how it goes!
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THE MYCOSMITH
Weekend mycologist


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 3
#28509580 - 10/18/23 02:23 PM (3 months, 10 days ago) |
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Quote:
Baba Yaga said: @CrimsonRambeler
agar just gives you more certainty and since cinctulus always looks so damn doggy I would say every bit of certainty that can be gained is gold in this case. Still early days so looking forward to some updates.
You probably have seen alaskaappalachian's post about his pan grow? Absolute beauty.
and a few images by mycosmith for later comparison. Really couldn't believe they could look like this.
Quote:
THE MYCOSMITH said: Hey Baba yaga. I expect you know that pan. Cinctulus is the blue Pearl Producer.

 I bought it as a spawn bag from a internet shop so i only have this culture. And No i Can get it to fruiting. I have try many different recipes of bulk substrat and different fruiting conditions with No luck. I have only succeeded to get a few pins on some Mac Merdin pan-cakes. I shut have cloned some. I just think i will let this culture die out.

Hey Baba Yaga. Nice to see you once again use my pics as an exampel. Makes me feel a little like a legend.

Back on track. A few mystery south africa that i harvested in the weekend. Only Two of them mature enough to print. But i also maked a few swabs for safety before printing. If any interest.
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