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the_prodigy
Be water my friend

Registered: 11/08/22
Posts: 23
Last seen: 2 days, 4 hours
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Quote:
Thanks! The super even canopies are a result of working with a clone and inoculating w LC, my pics from this summer are MS and show boxes that are more uneven, that's the point of growing out as many different iso's as you can manage, you just cherry pick the ones that perform well.
Sorry I didn't catch it, which Pans are those? BVI? Hawaian Copelandia from the growkits, what? Jeez you did some sick stuff with it, great job
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Mycomedicine888
Chief Executive Fruitcake



Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 6
#28500227 - 10/11/23 02:37 PM (3 months, 17 days ago) |
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Pretty baked on some sour gelato rn but here's my best answer:
Well lots of other things went wrong, but by flakey i wanna say that after three months in the fridge because of moving (syringes made from clean T2 clone dishes) it started with 4 out of 20 boxes aborting after the pins getting a cm or 2, getting worse on each run. Moving another two times and more fridgetime made my culture give only 1-4 below average boxes per 20 last year. My culture fridge accidentally got unplugged a couple of times as well, for possibly days on end, getting to 22-24°C a couple of times. So it seems that that sporeless superyielder is lost, although there still are some dishes from various years in the back of said fridge. It was potent af too, 0.7g dry already sent a few people trough a wormhole if i understood correct what they were trying to communicate to me through the speechimpairment of tripping balls, LOL. Now i would give no more than half a gram to anybody but a seasoned traveller. For the moment I feel i spent way too much labtime and resources on trying to revive it, that's why I'm interested in the stasis room T storage. I also grow Cordyceps which has the same problem as Pans only the sporeprints are even more shortlived. In my current setup it makes the most sense to do 10-20 boxes on each run, in 2021 it was a bit more, but let's not poke to much into doing things at that scale and focus on one 1,4x1,4m jartha with about 10 2gallon boxes when asking about it and act like we all just do small grows because we just really really love mushrooms, thanks!

My LC story: In 2021 i made clones off a multispore, coming from a print off a Copelandia cyanescens growkit bought in Amsterdam in 2020 and selected for the two fastest pinning ones. Clones were clean on T1, but made a T2 just to be safe. dishes went to LC (0.15% LME w tapwater filtered through a britta PC'd for 36mins). Growth after transfers looks cloudlike and fuzzy, being at its best between 9-18 days after inoc, agitated w a magnetic stirrer everyday for a minute at full speed after the first good growth appears 3-4 days after inoc. This gives a thick liquid with thousands of mycelial filaments floating around, congregating into the fuzzy cloud like shape when it settles down. Multiplying in this stage always gave further fast cloudlike fuzzy growth as in the original jar for about three weeks and growing super fast on grain and sub. Growing out that culture with 14day old LC is the pics on here from 2021 and my sig. Syringes made at this point worked perfect stored at room T for up to two weeks and after 3-4 months fridgetime and a move initially it seemed to do well, but i struggled on every run in that location with trich and cold draughts coming in from all directions as well. After +-25days the LC changes from fuzzy to algaelike, more slimy in a way, after which it pretty much stops growing. Growing out that stuff was very unpredictable, lots of boxes didn't fully colonize to the top and turn out duds after casing. On agar i verified it wasn't bacterial and it mutated fast af, sectoring out into stalling mycelium that usually makes some rings and doesn't budge anymore. Putting that on grain does absolutely nothing at all either. Most useful footnote in this story: grab spores on every run, although I loved that sporeless strain so much... Hmmmm
I moved into the place I'm now a year and a half ago and last year I threw the towel in the ring after months of struggling, but this year it seems im jumping all the hurdles and finding solutions, no LC work this year bc of a deadline on production, but there's more coming as things get more dialed in here and I'm also excited to try some semilanceata's this fall and hopefully get to share some results of an outdoor Psilo cyan grow So stay tuned 
Editing in some shroomporn so yall can have some wet dreams tonight Pan cyan truffles
  

Edited by Mycomedicine888 (10/12/23 07:44 AM)
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Mycomedicine888
Chief Executive Fruitcake



Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
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Re: Official Copelandia/Panaeolus Thread [Re: the_prodigy] 2
#28500269 - 10/11/23 03:10 PM (3 months, 17 days ago) |
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Thanks, your trays are looking really nice too, they look alot like the mushrooms that gave me my print. They were the Hawaiian Copelandia kit that was being sold in Amsterdam in 2020, it was a 1200ml kit that was marketed as being new at the time. Don't know if what they sell online is the exact same kit as I got, but the pictures are still the same. The mushrooms on the original kit looked alot like the ones in the middle of your sig and the ones being pictured on online vendors sites. It definitely is a different strain than the one that we could buy fresh in the smartshop back in the day, but that's pretty much all i got...
-------------------- I may have inhaled some of the spores....
    Copelandia cyanescens 2023
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Gorguss
Chaotic sums


Registered: 02/03/10
Posts: 637
Last seen: 7 hours, 53 minutes
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is microwaving field capacity peat/verm w/ lime for casing still viable?
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RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 1 hour, 1 minute
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss] 2
#28501739 - 10/12/23 02:38 PM (3 months, 16 days ago) |
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Was that a thing? I would think it would be hard to keep in pasteurization temps in the microwave.
If you need to pasteurize enough for just a few trays I use my dehydrator. Not a good method for large volumes, but I never need more than I can pasteurize on one or two trays.
Dehydrator casing pasteurization
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Mycomedicine888
Chief Executive Fruitcake



Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss]
#28501818 - 10/12/23 03:14 PM (3 months, 16 days ago) |
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Sorry I don't have a microwave, no tv either for that matter, those things really are detrimental for your health I pasteurized the peat casing on this run but have used untreated cocopeat in the past...
EDIT: DO NOT try untreated casing, unless you are 90% sure you will get away with it and are willing to take the 1/10chance
I've also succesfully induced pinning with baking paper on two occasions when not having casing ready for some reason, that's prob alot safer than microwaving peat. I'll dig to see if i took pics Procedure is as follows:
start: 1a have cake 1b have baking paper (i used brown baking paper) 1c Cut baking paper to the size of surface of cake 2 crumple to ball and 3 flatten out again 4 spray the fuck out of baking paper w 70% alcohol, both sides untill its soakin wet, balls deep, no mercy! 6a let slightly dry and ever so gently place on top of cake with avoiding excessive alcohol touching mycelium 8 pray to the shroomgods that this will work and they will guide your hands so you dont fuck it up (naked outside in a thunderstorm on your bare knees gives optimal result) 9 spray 2x a day (with WATER) until pins appear, then remove baking paper 10c go play outside so your shrooms dont get nervous or paranoid and abort from being stared at 24/7, 11 have shrooms 12b thank shroomgods by accidentally taking to much and slipping into catatonic stupor tripping balls so they can come to visit you and impart sacred knowledge 17 rehydrate & sleep 18a repeat the end
Best of luck 
@Roscoe: i like your thinking on that dryer casing method, that's a very nice tek! @ Baba: Maybe this is something to try on your no case tubs too? Very nice work BTW, its the same spirit as my approach, i just went martha with them
Edited by Mycomedicine888 (10/12/23 09:27 PM)
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Mycomedicine888
Chief Executive Fruitcake



Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
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Found this pic a few pages back:
 This illustrates the rings on flakey cultures i mentioned above, altough i wouldn't get that symetrical growth, it would mutate and sector out like crazy into nothingness really. Typically it will never reach the sides of the dish and it will never give good colonisation. The dishes will still look exactly the same months later, were good cultures will climb up the lids of your dishes and jars and will bruise completely blue if left sitting in dishes for a long time.
Looks like i hit my weekly upload limit so more next week probably
-------------------- I may have inhaled some of the spores....
    Copelandia cyanescens 2023
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odissey
Psychonaut


Registered: 01/19/21
Posts: 487
Loc: Europe
Last seen: 13 days, 11 hours
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These thought the tub was too limiting, so they decided to push their freedom out of the tub. Pan cyan texas in an unmod:
Edited by odissey (10/13/23 02:48 AM)
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: odissey] 2
#28502767 - 10/13/23 04:10 AM (3 months, 15 days ago) |
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Beauty
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Genome794
Spore-Whore-der & Agar-Addict


Registered: 07/20/22
Posts: 438
Loc: The Blue Planet
Last seen: 7 hours, 27 minutes
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Quote:
Mycomedicine888 said: Found this pic a few pages back:
 This illustrates the rings on flakey cultures i mentioned above, altough i wouldn't get that symetrical growth, it would mutate and sector out like crazy into nothingness really.
That's one of mine and it has yet to fruit in any way.
Quote:
Typically it will never reach the sides of the dish and it will never give good colonisation.
I beat the stalling by keeping some water from grain prep that had some dung added.
Some plates are slow, some are slower... In jars I shake them without mercy @ 3 days again at 7-10. If nothing comes I go back.
As I said, no fruit so far. I have been keeping at it because I don't/didn't have an alternative Pan. That is about to change, my Free Spore Espana prints are almost here... So maybe I can give up on this one.
794
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tree frog
eats bugs


Registered: 09/14/23
Posts: 458
Loc: lives in trees
Last seen: 4 minutes, 13 seconds
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Re: Official Copelandia/Panaeolus Thread [Re: Genome794]
#28502844 - 10/13/23 06:56 AM (3 months, 15 days ago) |
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Was planning to wait until the new year but couldn't help it and streaked four plates of lme agar with pan. bisporus this morning.
Should I make some water agar for transfers? Or would grain water agar be better?
I've seen a lot of people are having issues with this stuff stalling, and I'd like to have the right plates ready for my first transfers.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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CrimsonRambeler
Stranger



Registered: 11/10/22
Posts: 20
Last seen: 1 day, 1 hour
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28502978 - 10/13/23 08:57 AM (3 months, 15 days ago) |
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I shook up a spore to grain with Panaeolus cincticulus only five days or so after Innoculating. I didn't know Cyans grew truffles, but that's what I'm growing cincticulus for, and as you can see in the jar it's already started popping up in a few places, the beginning of sclerocia. The jar pictured here is two weeks after spore to grain and about a week after the shake. Bounced back just fine, hoping for a successful rest of the grow and many many transfers.



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ruawakeyet

Registered: 05/03/21
Posts: 1,862
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I'm not so sure that is the start of sclerotia. Considering it's spore to grain, and by the looks of it, I'm sorry to say that looks like the start of contamination.
Keep a close eye on it over the next 2 to 3 days, and if there is any question, post up some additional pictures for others to weigh in.
I would also like to encourage you to get into agar. I wish I had not avoided it for so long. With a little practice, it will really step up your game to the next level.
Quote:
tree frog said: Was planning to wait until the new year but couldn't help it and streaked four plates of lme agar with pan. bisporus this morning.
Should I make some water agar for transfers? Or would grain water agar be better?
I've seen a lot of people are having issues with this stuff stalling, and I'd like to have the right plates ready for my first transfers.
If you have enough plates to use, I would suggest making both water agar and GWA, and/or LME agar. Take transfers to all of them, and see what works best for you. Some have great success with GWA, others not so much. Same can be said for LME. Unfortunately, it will take some trial and error to see what works best for you. The odds will be in your favor if you diversify your methods at this time.
BTW, water agar is primarily a tool used to help clean up bacterial cultures or clone tissue. It isn't always necessary, but won't hurt as long as you take a final transfer back to a nutrient agar before using it for inoculation. If that final transfer is still clean on nutrient agar, you should be good to go for inoculation.
Edited by ruawakeyet (10/13/23 05:08 PM)
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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@CrimsonRambeler
agar just gives you more certainty and since cinctulus always looks so damn doggy I would say every bit of certainty that can be gained is gold in this case. Still early days so looking forward to some updates.
You probably have seen alaskaappalachian's post about his pan grow? Absolute beauty.
and a few images by mycosmith for later comparison. Really couldn't believe they could look like this.
Quote:
THE MYCOSMITH said: Hey Baba yaga. I expect you know that pan. Cinctulus is the blue Pearl Producer.

 I bought it as a spawn bag from a internet shop so i only have this culture. And No i Can get it to fruiting. I have try many different recipes of bulk substrat and different fruiting conditions with No luck. I have only succeeded to get a few pins on some Mac Merdin pan-cakes. I shut have cloned some. I just think i will let this culture die out.
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odissey
Psychonaut


Registered: 01/19/21
Posts: 487
Loc: Europe
Last seen: 13 days, 11 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 2
#28503966 - 10/14/23 02:49 AM (3 months, 14 days ago) |
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Quote:
Baba Yaga said: Beauty
Thanks Baba! Your pans in monotubs thread was a huge encouragement to give this species a new chance since currently I dont have the mental space or time to set up and manage a jcm. I wanted to have a proper high dose trip to see what this species is all about again because my last proper grow ended up with no potency at all, and the one before that... Well, let's just say I was a little unprepared for what hit me  also curious to try semp/pan mix
Edited by odissey (10/14/23 07:50 AM)
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Bismillah
Scharf im Schlafanzug


Registered: 07/11/22
Posts: 169
Last seen: 5 hours, 15 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: odissey] 2
#28503995 - 10/14/23 04:26 AM (3 months, 14 days ago) |
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Hey, I got my first pan cyan BVI fruits! This culture has been slow all the way from plates to grain. Germ plates started early July, nocced up grain August 8, over 2 months ago. Spawned to bulk (horse/moose poop, straw, coir) 1 month ago, cased 9 days ago and I stuck it in the FC a day later.

One cap is black, probably because I misted a little the other day. I don't think that was necessary though, the surface is quite wet. I also got a pink spot that doesn't look very good. The lion's mane is not as dense as I would like, I guess it wants more FAE or less humidity. It's soaked actually..
Anyway, this gives me great hope. I have some LC that I also used on some pan cakes and a few EF cakes, and these are colonizing nicely. Thanks to everyone for sharing their knowledge in this thread!
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smalltalk_canceled
Babnik



Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 5 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah]
#28504339 - 10/14/23 12:52 PM (3 months, 14 days ago) |
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12 bags of pans 100% colonized havent opened the bags in any way but they are looking drier waiting for orders from shroomery gang
they are all a clone based on this:






its 12 1,5 kgs of manure, coir, chaff, some grains from the inoculation, gypsum/lime and some flour
If placed naked in a tub, it will dry out from all sides
If monotubed, it will abort like the last time?
gonna try additional heigh above them, and high humidity
if advicing, im not able to set up a dynamic chamber and its an attempt at cloning a passive setup pan culture
==
Im casing the FIRST (1) one, intact in coir, in a tub, with good space, either as a covered potato with a small coir covering in the bottom, or buried "iceberg" style - where the coir reaches the edges of the top of the surface, but not much more after that
what do you think?
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (10/14/23 12:53 PM)
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kirkeng
Stranger


Registered: 02/14/21
Posts: 807
Loc: Lost
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Are you sure those are pans my friend? They look a lot like tamps or mexicana to me, the textured stems and cap color, as well as spiraled stems on some are typical in what I see for tamps/mexicana.
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smalltalk_canceled
Babnik



Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 5 hours
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
#28504402 - 10/14/23 02:06 PM (3 months, 14 days ago) |
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nobody in the subtr/pan thread has been able to ID been through that before
you think they are tamps? then for purposes of saving the batch,
what will cause tamps to abort from the cap and downwards during fruiting, what break in conditions would cause that?
what conditions would it half fruit in, but then mostly abort near the end
AND/OR
how to spawn tamp substrates from bags to tubs ?
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (10/14/23 02:10 PM)
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kirkeng
Stranger


Registered: 02/14/21
Posts: 807
Loc: Lost
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Tamps and mexicana have done that in high co2 and dry conditions for me. Excessive water and good air flow manages them healthy in my experience.
When I do tamps and mexicana I spawn them to 30/70 manure/coir and run them in unmodified tubs with significant air gaps around the lid, misting very heavily twice a day. There are significant fluctuations in humidity throughout the day but remains overall wet throughout. If they are in fact tamps they seem dry to me. I like to have them basically dripping with water for a good section of the day. They obviously will dry on the surface at some points but not for very long maybe a few hours at most before getting re drenched. This is just my opinion though so take it or leave it.
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