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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
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Quote:
Mycomedicine888 said: A half myco quart to each sounds like chinese to me sorry 
It's two700ml jars of spawn to +-4,5L of sub, that would make it almost 1/4.5 ratio Spawn is rye with gypsum, finding good clean grain is harder than you would think, tried at least 7 different sources before finding the one i got now Sub is composted cow manure (stable manure with lots of straw already in it)+verm(+gypsum) in a ratio fluctuating between 6/4 and 4/6,depending on how wet the shit is Casing on this run was the old classic peat/verm w lime, first time i've done this In the past i used cocopeat/verm, have to run more tests to make sure but i got better pinsets with that, it could be genetics too... They can go for 5flushes without contam, my martha is setup in my living room so def not sterile. Airflow in and out is filtered though. As long as everything is super clean untill your sub is fully colonized they are quite robust to anything except sciarids for me, those really suck when they get in
As for the containers, they seem to work best when they're about 2inches taller than about 3inches of sub, but it does make for easier harvest when the sub is closer to the rim of the container. My favourite are the shallower ones for easier picking, but best performance so far are these two:








 Clear plastic one is12L, the black ones 9L, they had about 6-8L of sub if i remember correct...
Best of luck
Jesus flying cock! I've never seen anything even remotely similar! Where the hell did you get a Pan culture that produces Cube-sized fruits and makes consistent canopies?
  
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Bismillah
Scharf im Schlafanzug


Registered: 07/11/22
Posts: 169
Last seen: 3 hours, 25 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente] 4
#28498699 - 10/10/23 10:53 AM (3 months, 18 days ago) |
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I'm currently fruiting lion's mane in my pan mini mono, harvested twice:

It was actually quite useful, the coral growth I got in the start told me I didn't have enough FAE. The pan culture is most likely a dud. The cased box has been in there for 5 days, colonized for a month prior to that with weak growth.
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Mycomedicine888
Chief Executive Fruitcake



Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 7
#28498748 - 10/10/23 12:04 PM (3 months, 18 days ago) |
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The size of cubes LOL
Guess my biggest pans are close to my smallest cubes
 
But pans:
       
VS cubes:
     
      My cubes get the size of amanita's 
It's from spores off a kit bought in Amsterdam in 2020, my best guess after looking at 100's of pics is it's from the Pan cyan Hawaii that sporeworks carried about ten years ago... The cracked caps one in my sig was the dominant pheno on that culture, it didn't drop spores which was soooo nice, about 20% came out as the big whoppers that didn't crack their caps when ripe Unfortunately that culture seems to not have survived moving shop 4times over the past 3 years. I'm reading up on stasis storage in distilled water at room temperature like in this thread: https://www.shroomery.org/forums/showflat.php/Number/28358195#28358195 since pan cultures dont seem to like the fridge that much, does anyone here have two cents on that? Also a 12°C fridge might work, Stamets mentions something about it concerning another fast scenescing tropical species (Volvariella)
-------------------- I may have inhaled some of the spores....
    Copelandia cyanescens 2023
Edited by Mycomedicine888 (10/10/23 12:19 PM)
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Baba Yaga
ā„ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Fantastic looking trays and constant results. Beautiful.
So you are getting approx. 25g dried grams from 1.4 liters of spawn? How many flushes on average til you get to this yield? 
I have good success with long term storage in LC with a low nutrient content (0.05% - 0.1% LME). So far cultures survived 2 years @ room temp. It also fits my workflow as I can set aside a couple of good looking LCs to store and can use them straight away when taking them out to expand and inoclulate.
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 10 minutes, 23 seconds
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Maybe you just have really small hands...
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Mycomedicine888
Chief Executive Fruitcake



Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 2
#28498810 - 10/10/23 01:07 PM (3 months, 18 days ago) |
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That's on 3flushes, i don't wheigh the second or third ones but i'd guestimate it's 50/35/15% ish of total yield My biggest 1stflush was +700g fresh off one of those 11L boxes For me they seem to give their best on the first flush with the latter ones throwing out more oddballs so after three flushes they're not worth sitting around taking up space And the grainjars are 4/5ths filled so it's closer to 1L than 1.4, maybe 1.2L? I eyeball most things tbh, except with agar Fastest and best strain was in 2021, the one in my sig, 9days LC, 9days grain w 1shake 5 days in and then 6-9 days for full colonisation and 9 again after casing for first pins casing was cocopeat/verm, so 33 days from 1stdish to 1stflush
This year it took em 11days to first pins after casing, still get prepin-anxiety when they're about to get there That's the moment to ignore them completely and go do something else They seem to stall when you look at them constantly hehe
Edited by Mycomedicine888 (10/10/23 01:37 PM)
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Mycomedicine888
Chief Executive Fruitcake



Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
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Re: Official Copelandia/Panaeolus Thread [Re: Tiamo] 3
#28498843 - 10/10/23 01:33 PM (3 months, 18 days ago) |
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IDK man, this is a commercial kit from holland
 And this is it's clone 6months later


Definitely never got any 60g shrooms off the kits you can buy you tell me
-------------------- I may have inhaled some of the spores....
    Copelandia cyanescens 2023
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 18 hours
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Quote:
Mycomedicine888 said: A half myco quart to each sounds like chinese to me sorry 
It's two700ml jars of spawn to +-4,5L of sub, that would make it almost 1/4.5 ratio Spawn is rye with gypsum, finding good clean grain is harder than you would think, tried at least 7 different sources before finding the one i got now Sub is composted cow manure (stable manure with lots of straw already in it)+verm(+gypsum) in a ratio fluctuating between 6/4 and 4/6,depending on how wet the shit is Casing on this run was the old classic peat/verm w lime, first time i've done this In the past i used cocopeat/verm, have to run more tests to make sure but i got better pinsets with that, it could be genetics too... They can go for 5flushes without contam, my martha is setup in my living room so def not sterile. Airflow in and out is filtered though. As long as everything is super clean untill your sub is fully colonized they are quite robust to anything except sciarids for me, those really suck when they get in
As for the containers, they seem to work best when they're about 2inches taller than about 3inches of sub, but it does make for easier harvest when the sub is closer to the rim of the container. My favourite are the shallower ones for easier picking, but best performance so far are these two:








 Clear plastic one is12L, the black ones 9L, they had about 6-8L of sub if i remember correct...
Best of luck
Dude...that is fucking insane.
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WeavieWonder
Time Travel Sucks



Registered: 08/18/16
Posts: 1,455
Loc: USA
Last seen: 1 month, 3 days
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Re: Official Copelandia/Panaeolus Thread [Re: Asura] 1
#28499350 - 10/10/23 08:07 PM (3 months, 17 days ago) |
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
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Re: Official Copelandia/Panaeolus Thread [Re: WeavieWonder] 2
#28499410 - 10/10/23 09:07 PM (3 months, 17 days ago) |
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Man those caps are fat if that was in pic of the day Iād just have browsed right by thinking it was cubes.
Are those trays spore or clone?
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LAGM2020     
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 9,288
Loc: South Africa
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a] 1
#28499523 - 10/11/23 12:05 AM (3 months, 17 days ago) |
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Beautiful
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astra
Mycelia-Of-House-Fungi


Registered: 12/24/21
Posts: 81
Loc: Japan
Last seen: 4 hours, 43 minutes
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Quote:
Mycomedicine888 said: A half myco quart to each sounds like chinese to me sorry 
It's two700ml jars of spawn to +-4,5L of sub, that would make it almost 1/4.5 ratio Spawn is rye with gypsum, finding good clean grain is harder than you would think, tried at least 7 different sources before finding the one i got now Sub is composted cow manure (stable manure with lots of straw already in it)+verm(+gypsum) in a ratio fluctuating between 6/4 and 4/6,depending on how wet the shit is Casing on this run was the old classic peat/verm w lime, first time i've done this In the past i used cocopeat/verm, have to run more tests to make sure but i got better pinsets with that, it could be genetics too... They can go for 5flushes without contam, my martha is setup in my living room so def not sterile. Airflow in and out is filtered though. As long as everything is super clean untill your sub is fully colonized they are quite robust to anything except sciarids for me, those really suck when they get in
As for the containers, they seem to work best when they're about 2inches taller than about 3inches of sub, but it does make for easier harvest when the sub is closer to the rim of the container. My favourite are the shallower ones for easier picking, but best performance so far are these two:








 Clear plastic one is12L, the black ones 9L, they had about 6-8L of sub if i remember correct...
Best of luck
Jesus christ, dude.... Thought they were cubes for a hot minute wow
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Kunta Kente
BroBot 9k



Registered: 02/24/08
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Loc: Hot Texas
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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente]
#28499572 - 10/11/23 01:49 AM (3 months, 17 days ago) |
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Quote:
Kunta Kente said: Do any of you guys use a Martha setup? I'm interested in messing around with lions mane and oysters as well as pans, so I didn't know if that would be a reasonable idea or if you just do monotub like the 3.0 thread. I won't be able to exhaust out of the apartment. In a few months I'll have more room. Currently trying my first few pans in unmodded tubs.
Bump
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Everything I have typed is purely fiction, and is purely for personal amusement. All images I've posted are not mine and I am simply posting interesting things I found on the Google.
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Baba Yaga
ā„ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente] 2
#28499577 - 10/11/23 02:00 AM (3 months, 17 days ago) |
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Quote:
Mycomedicine888 said:
Quote:
Kunta Kente said: Do any of you guys use a Martha setup? I'm interested in messing around with lions mane and oysters as well as pans, so I didn't know if that would be a reasonable idea or if you just do monotub like the 3.0 thread. I won't be able to exhaust out of the apartment. In a few months I'll have more room. Currently trying my first few pans in unmodded tubs.
Yeah i've grown Lion's Mane, Shiitake,all sorts of Oysters, a few different strains of Reishi, cubes and pans in bigger martha style setup (1,4x1,4m tents), it definitely gives me good yields and big fruits, but good cultures are half of the work i'd say... They all require slightly different conditions though, so having one tent per species would be ideal and most oysters sporulate very heavy so def filter your exhaust going into your living space and regularly change the filters. To stay on topic: the pans in the martha give an average yield 25g dry/4,5l of sub, which is 5 more than i got out of monotubs Best of luck!
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Mycomedicine888
Chief Executive Fruitcake



Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
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Thanks you guys ,
Yeah going tub to martha was a gamechanger for me, Don't think i could explain it better than jake in his excellent thread: JOC TEK Panaeolus cyanescens Although mine does run with inkbirds, so i dial it in differently than he does. Recently i heard a very good podcast by him on yt, at some point they mention something along the lines of: "think of mushrooms growing as evaporating water pulling/pushing the tissue out", that's a pretty good picture imo, you need the constant evaporation for even growth, just defferent species ask for different evap rates. You also need all that evaporated water to leave your FC, the speed at which this happens differs on what mushroom you're aiming to fruit. I also use only rye for spawn, no other grains and apply fruiting conditions immediately after casing, no consolidating casing period Further I can recommend Baba's advice learn to toss shit out before it fucks up the flow of your grow and grow out enough stuff to find what works well in your setup and workflow...
Quote:
Bismillah said: I'm currently fruiting lion's mane in my pan mini mono, harvested twice: It was actually quite useful, the coral growth I got in the start told me I didn't have enough FAE. The pan culture is most likely a dud. The cased box has been in there for 5 days, colonized for a month prior to that with weak growth.
Although pinning conditions are very close to eachother, lions mane needs slower evaporation than pans, your box looks pretty wet and are they both cased? The casing acts as a buffer to hold a superhigh humidity microclimate while still allowing for lots of evap, the water has to be able to get out too. Maybe you could increase the size of your ventholes or change the filtermaterial? I switched to cheap ass vacuumfilterbags for all my FC filters, they work great for me
    
Quote:
smalltalk_canceled said: Im gonna need help the following days Just brought home the first true pan project ever 10 substrate bags, manure in the mix, now landing in a warm room after 2 days in a medium temp car. I have no idea how to spawn these. It's a clone taken from a passive tub
What do you plan to do with that? what do you need advice with? And what does it mean 'taken from a passive tub'?
Quote:
Baba Yaga said: Fantastic looking trays and constant results. Beautiful. I have good success with long term storage in LC with a low nutrient content (0.05% - 0.1% LME). So far cultures survived 2 years @ room temp. It also fits my workflow as I can set aside a couple of good looking LCs to store and can use them straight away when taking them out to expand and inoclulate.
Thanks, nice work on that LC revival tek, do you get the same yields after reviving LC's? Mine seemingly did fine in LC syringes in the fridge for 3months in 2021, but it got flakey fast after that. This spring I decided to stop trying to revive it after spending 100's and 100's of dishes on it the last year. And oh, man i make my LC at 0.15%, i dumped out sooo many jars after they stop growing... For me they change in appearance at some point, turning from cloud like to algae like and after that they get flakey in culture. I too prefer LC since I work in a SAB as well, and for efficiency, uniformity, speed and ease nothing beats it. You also mention selection criteria, harvesting ease is something i select for too, some strains rip up entire chunks of the cake when you pull em and others snap their stem flush at substrate level, I really don't like it when the cake looks like a minefield after war... Very nice semp thread you got there as well, gonna have a crack at those this fall too
Quote:
A.k.a said: Man those caps are fat if that was in pic of the day Iād just have browsed right by thinking it was cubes. Are those trays spore or clone?
Thanks! The super even canopies are a result of working with a clone and inoculating w LC, my pics from this summer are MS and show boxes that are more uneven, that's the point of growing out as many different iso's as you can manage, you just cherry pick the ones that perform well.
And on the scent: mine smell fruity and zesty with the spawn smelling even stronger, giving an almost fizzing sensation in your nose? It's uncomparable, sometimes it makes me sneeze like smoking a really strong weed. The stronger they smell the better they perform and the more potent they tend to be. Cubes stink like wet dog for me as someone mentioned before.... from agar to fruit they stink!
Have a fruitfull day y'all
-------------------- I may have inhaled some of the spores....
    Copelandia cyanescens 2023
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Mycomedicine888
Chief Executive Fruitcake



Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
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And oh yeah, be prepared to fail hard an often in the beginning, and again, harder than ever before just when you start to think you know your shit, but don' let it discourage you. I had to start 3 times over from spore this year, with first two g dry/ 10 of those boxes then only one and then not even reaching pinning. Fourth run went flawless and was the usual 25/box average even though it was MS and some trays only gave a handfull. Never had bacterial contam coming from inside my agar before, took 3 runs to figure it out and adjust and also had to turn my FC from neg pressure to pos pressure to keep out sciarids that made me shut down my whole operation last year after them destroying 5 crops moments before harvest. Every place comes with it's own hurdles and good cultivation is identifying them and figuring out solutions preventing them from fucking up the flow of your operation, that's the puzzle that makes this so much fun to me. Sometimes it just comes down to who's the most stubborn, the cultivator or the contaminant
Edited by Mycomedicine888 (10/11/23 07:21 AM)
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ReverendMyc

Registered: 03/29/19
Posts: 1,507
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Dropping some solid knowledge! I am really enjoying and encouraged by your posts.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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Bismillah
Scharf im Schlafanzug


Registered: 07/11/22
Posts: 169
Last seen: 3 hours, 25 minutes
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Quote:
Mycomedicine888 said: Although pinning conditions are very close to eachother, lions mane needs slower evaporation than pans, your box looks pretty wet and are they both cased? The casing acts as a buffer to hold a superhigh humidity microclimate while still allowing for lots of evap, the water has to be able to get out too. Maybe you could increase the size of your ventholes or change the filtermaterial? I switched to cheap ass vacuumfilterbags for all my FC filters, they work great for me
Well, the lion's mane is pinning in the bag, right? All this talk about evaporation is confusing to me since I can't really observe evaporation taking place. I get that you need <100% RH for evaporation to happen, but even that is not really measurable at the substrate surface. I get a constant supply of dry air through an aquarium pump, and air is exchanged every 15-20 minutes using fan+heater from an old food dehydrator. I hope this cycle will create evaporation without drying out the substrate.
The hair hygrometer you see in the pic is at the substrate level, stuck to the wall full of droplets. It's not fast enough to register a humidity drop due to air exchange, so it shows 100% RH all the time. Higher up on the box I have an Atlas Scientific Ezo-Hum sensor that shows 85-90% RH, and it drops to 65-70% for 1-2 minutes when the air is exchanged.
Inside the box I have a small fan to distribute the mist from an ultrasonic fogger at the bottom of the box. Before I added the fogger, the hair hygrometer showed around 80-85% RH.
I also have a jcm style tub set up. I was afraid it would be too cold in my basement for this, but then I see coversall and pesa fruit in cold environments too. Time will tell.
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astra
Mycelia-Of-House-Fungi


Registered: 12/24/21
Posts: 81
Loc: Japan
Last seen: 4 hours, 43 minutes
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Quote:
Mycomedicine888 said: And oh yeah, be prepared to fail hard an often in the beginning, and again, harder than ever before just when you start to think you know your shit, but don' let it discourage you. I had to start 3 times over from spore this year, with first two g dry/ 10 of those boxes then only one and then not even reaching pinning. Fourth run went flawless and was the usual 25/box average even though it was MS and some trays only gave a handfull. Never had bacterial contam coming from inside my agar before, took 3 runs to figure it out and adjust and also had to turn my FC from neg pressure to pos pressure to keep out sciarids that made me shut down my whole operation last year after them destroying 5 crops moments before harvest. Every place comes with it's own hurdles and good cultivation is identifying them and figuring out solutions preventing them from fucking up the flow of your operation, that's the puzzle that makes this so much fun to me. Sometimes it just comes down to who's the most stubborn, the cultivator or the contaminant 

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Baba Yaga
ā„ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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so true
Quote:
Mycomedicine888 said:
Quote:
Baba Yaga said: Fantastic looking trays and constant results. Beautiful. I have good success with long term storage in LC with a low nutrient content (0.05% - 0.1% LME). So far cultures survived 2 years @ room temp. It also fits my workflow as I can set aside a couple of good looking LCs to store and can use them straight away when taking them out to expand and inoclulate.
Thanks, nice work on that LC revival tek, do you get the same yields after reviving LC's? Mine seemingly did fine in LC syringes in the fridge for 3months in 2021, but it got flakey fast after that. This spring I decided to stop trying to revive it after spending 100's and 100's of dishes on it the last year. And oh, man i make my LC at 0.15%, i dumped out sooo many jars after they stop growing... For me they change in appearance at some point, turning from cloud like to algae like and after that they get flakey in culture. I too prefer LC since I work in a SAB as well, and for efficiency, uniformity, speed and ease nothing beats it. You also mention selection criteria, harvesting ease is something i select for too, some strains rip up entire chunks of the cake when you pull em and others snap their stem flush at substrate level, I really don't like it when the cake looks like a minefield after war...
Not exactly sure what you mean by flaky. For me pan LC does tend to stop growing cloud like after a few shakes even when it's fresh, more like little cauliflower heads but it doesn't seem to be detrimental, it just doesn't break up that easily. Still too early to comment on yields after long term storage and tbh it's not so much for the preservation of a clone but another option to preserve a species and variety and I'm happy if I can get a small grow going and a few fresh prints to start from zero, prints in storage don't seem to last that long. I can see that harvesting ease is a big plus if I had to handle that many trays. I just harvest till I have ~50g dry for of a variety for reference and the rest gets recycled back to mother nature (compost).
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