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Babnik
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Re: Official Copelandia/Panaeolus Thread [Re: Mycomedicine888]
    #28495318 - 10/07/23 10:25 AM (3 months, 21 days ago)

Im gonna need help the following days

Just brought home the first true pan project ever

10 substrate bags, manure  in the mix, now landing in a warm room after 2 days in a medium temp car.

I have no idea how to spawn these.

It's a clone taken from a passive tub


--------------------
Willpower is the one true virtue



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InvisibleTheAtlanteanS
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Registered: 06/25/18
Posts: 1,003
Loc: Milkyway Galaxy, Laniakea Supe...
Re: Official Copelandia/Panaeolus Thread [Re: Mycomedicine888]
    #28495488 - 10/07/23 01:22 PM (3 months, 21 days ago)

Beautiful flushes.


--------------------

Meditation is passive learning. Tabernacling with the one infinite Creator is passive learning. It is the learning from listening to the voice one cannot hear which answers questions which one cannot ask. The process cannot be known and cannot come into consciousness in local sense, yet the learning is there. And the strength of spirit and will which comes from this learning is helpful.
.

:egyptian:www.templeofhermes.org:egyptian:


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InvisibleShrimps
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I'm a teapot

Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
Re: Official Copelandia/Panaeolus Thread [Re: TheAtlantean]
    #28495541 - 10/07/23 02:31 PM (3 months, 21 days ago)

So guys, I grew some Pan. cyan peace rive a few months back (got like 3gr dried, maybe a bit more) and I was wondering, anybody can point me out do a good dose? I mean, I am used to cubes but no experience with these.


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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OfflineTrKyNcKmSk
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps]
    #28496069 - 10/08/23 01:22 AM (3 months, 20 days ago)



This is my first attempt at growing panaeolus mushrooms. I 'nocced up some agar, but in order to concentrate the syringes I let the spores sink to the bottom and shot up a few jars. This jar is showing some growth but I am unfamiliar with how the mycelium is supposed to look... The variety I have in this jar is Pan Cambo Goliath. Is that mycelium or mold? Never got mold from a syringe from said vendor before and I prepped the grain perfect. My jars with agar are doing great, I just need to look at some more pics and get someone's opinion. Thanks. I want to take some grains back to agar when the jar is done so I'm hoping and praying that's mycelium.


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Offlineastra
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28496186 - 10/08/23 05:41 AM (3 months, 20 days ago)

Peace Rivers are lovely. If this is your first time dosing pans, but have done cubes previously, 1 gram should suffice... or 0.5g for the fully inexperienced, imho. Remember you can always take a bit more next time (give yourself 14 days as a bare minimum between trips but the longer you rest the better ime). Safe travels :nyan:


--------------------
:ancientaliens:


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Offlineastra
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28496187 - 10/08/23 05:42 AM (3 months, 20 days ago)

Quote:

Shrimps said:
So guys, I grew some Pan. cyan peace rive a few months back (got like 3gr dried, maybe a bit more) and I was wondering, anybody can point me out do a good dose? I mean, I am used to cubes but no experience with these.





Peace Rivers are lovely. If this is your first time dosing pans, but have done cubes previously, 1 gram should suffice... or 0.5g for the fully inexperienced, imho. Remember you can always take a bit more next time (give yourself 14 days as a bare minimum between trips but the longer you rest the better ime). Safe travels :nyan:


--------------------
:ancientaliens:


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Offlineastra
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Re: Official Copelandia/Panaeolus Thread [Re: Murphy07] * 5
    #28496192 - 10/08/23 06:06 AM (3 months, 20 days ago)

Quote:

Murphy07 said:
Hello again my fellow pan Brothers. I have a question regarding overlay. Only thread I could find was from rhe GOATS assura and blue helix but didn't really nail down an answer I've got this gnarly overlay, and idk what causes it. This my 2nd or 3rd tray that's gotten this and sometimes it's fruits sometimes it doesn't. I've had about 6 successful trays in the last few weeks before these and curious is if anyone else has something similar.




I've had them before and and pretty recently as well. Nothing to do with 'lack of fae' as you might find the floating iceberg type of stroma will show up in both automated grow tents and jcm chambers for example. Its more of a genetic flaw, mostly seen with jamaican, estero and bvi varieties. Random af tbh.













--------------------
:ancientaliens:


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Offlineastra
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Re: Official Copelandia/Panaeolus Thread [Re: sleepydave]
    #28496195 - 10/08/23 06:12 AM (3 months, 20 days ago)

Quote:

sleepydave said:
Some Pan Cyan FL "Blue Springs" red spore that filled out quite nicely, along with some Pan Cyan Australia "Grasstree" creeping in on the right of the photo.








Beautiful Blue Springs:heart: :manofapproval:


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:ancientaliens:


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OfflineTrKyNcKmSk
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Re: Official Copelandia/Panaeolus Thread [Re: astra] * 1
    #28496657 - 10/08/23 03:05 PM (3 months, 20 days ago)

Hopefully these pics are better. Does it look like Pan Cambo Goliath mycelium, or mold. I'm a noob. Now I'm gonna go back and read the whole thread. Thanks.





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InvisibleShrimps
Traveler
I'm a teapot

Registered: 10/13/22
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Loc: Under the sea
Re: Official Copelandia/Panaeolus Thread [Re: astra] * 1
    #28497207 - 10/09/23 12:44 AM (3 months, 19 days ago)

Quote:

astra said:
Peace Rivers are lovely. If this is your first time dosing pans, but have done cubes previously, 1 gram should suffice... or 0.5g for the fully inexperienced, imho. Remember you can always take a bit more next time (give yourself 14 days as a bare minimum between trips but the longer you rest the better ime). Safe travels :nyan:





Thanks will do <3


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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OfflineKunta Kente
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps]
    #28497235 - 10/09/23 01:59 AM (3 months, 19 days ago)

Do any of you guys use a Martha setup? I'm interested in messing around with lions mane and oysters as well as pans, so I didn't know if that would be a reasonable idea or if you just do monotub like the 3.0 thread. I won't be able to exhaust out of the apartment. In a few months I'll have more room. Currently trying my first few pans in unmodded tubs.


--------------------

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.


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Offlineodissey
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Re: Official Copelandia/Panaeolus Thread [Re: astra] * 1
    #28497242 - 10/09/23 02:34 AM (3 months, 19 days ago)

Quote:

astra said:
Quote:

Murphy07 said:
Hello again my fellow pan Brothers. I have a question regarding overlay. Only thread I could find was from rhe GOATS assura and blue helix but didn't really nail down an answer I've got this gnarly overlay, and idk what causes it. This my 2nd or 3rd tray that's gotten this and sometimes it's fruits sometimes it doesn't. I've had about 6 successful trays in the last few weeks before these and curious is if anyone else has something similar.




I've had them before and and pretty recently as well. Nothing to do with 'lack of fae' as you might find the floating iceberg type of stroma will show up in both automated grow tents and jcm chambers for example. Its more of a genetic flaw, mostly seen with jamaican, estero and bvi varieties. Random af tbh.















Beautiful trays! I would also like to add that I am currently working with a culture which overlaid BADLY. First tub, I cased as soon as the sub was colonized and the overlay got to like 7 cm thick all over. Second one, I let it sit for longer in the jar, let sit for more than a week after it fully colonized the sub and it seemed like it was not moving anymore and then cased. It was still overlaying a lot, although not as much as the previous tub. Still, it just sit there for 2 weeks with no development at all so I assumed that tub was gone. I had read that people messing with the overlay did not have any return out of it, but I know pans need cycles of evaporation to pin and I believe the overlay was preventing that. So, I manually removed the overlay by pinching it with a flame sterilised scissors, and to my surprise, the very next day pins! Ofc it could have been that it would have pinned anyway, but because there was no activity at all for 2 weeks and it pinned immediatelly after my intervention, I believe it was causal.
All in an unmod and the sub is coir/manure pellets so nothing optimal even.



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Offlineastra
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Registered: 12/24/21
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Re: Official Copelandia/Panaeolus Thread [Re: odissey]
    #28497278 - 10/09/23 05:38 AM (3 months, 19 days ago)

Quote:

odissey said:
Quote:

astra said:
Quote:

Murphy07 said:
Hello again my fellow pan Brothers. I have a question regarding overlay. Only thread I could find was from rhe GOATS assura and blue helix but didn't really nail down an answer I've got this gnarly overlay, and idk what causes it. This my 2nd or 3rd tray that's gotten this and sometimes it's fruits sometimes it doesn't. I've had about 6 successful trays in the last few weeks before these and curious is if anyone else has something similar.




I've had them before and and pretty recently as well. Nothing to do with 'lack of fae' as you might find the floating iceberg type of stroma will show up in both automated grow tents and jcm chambers for example. Its more of a genetic flaw, mostly seen with jamaican, estero and bvi varieties. Random af tbh.















Beautiful trays! I would also like to add that I am currently working with a culture which overlaid BADLY. First tub, I cased as soon as the sub was colonized and the overlay got to like 7 cm thick all over. Second one, I let it sit for longer in the jar, let sit for more than a week after it fully colonized the sub and it seemed like it was not moving anymore and then cased. It was still overlaying a lot, although not as much as the previous tub. Still, it just sit there for 2 weeks with no development at all so I assumed that tub was gone. I had read that people messing with the overlay did not have any return out of it, but I know pans need cycles of evaporation to pin and I believe the overlay was preventing that. So, I manually removed the overlay by pinching it with a flame sterilised scissors, and to my surprise, the very next day pins! Ofc it could have been that it would have pinned anyway, but because there was no activity at all for 2 weeks and it pinned immediatelly after my intervention, I believe it was causal.
All in an unmod and the sub is coir/manure pellets so nothing optimal even.






Agreed. Seemingly nice looking cultures on agar or grain might flop once they hit bulk sub. Interestingly enough, i've taken similar approach by trimming the thickest stroma and placing it aside for brewing tea purposes since its pretty active itself. Such tray will continue to overlay but not as bad as the first time and by day 5 or 6, pins will follow...up to 4 weeks ime. The fruits will get thicker too. What i've seen so far is, the afflicted trays tend to do better with a bit of stroma left as it keeps the surface conveniently moist.
That said, its 1 or 2 trays out of 50 making the whole situation uncommon, fortunately.


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OfflineGenome794
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Registered: 07/20/22
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Re: Official Copelandia/Panaeolus Thread [Re: astra]
    #28497707 - 10/09/23 02:16 PM (3 months, 19 days ago)

Still working... While I wait for prints from FSE.

I doubt that I am going to win with this culture but keep going while I wait.

Don't really know what to do with this... A month old.


In desparation I did some grass only sub...


The pics look more white with denser mycelium than it looks IRL.

Looking at recent pics of your great grows I think I may have "isolated" a good healthy mold.:sad:


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OfflineTrKyNcKmSk
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Re: Official Copelandia/Panaeolus Thread [Re: Genome794]
    #28497961 - 10/09/23 05:59 PM (3 months, 19 days ago)

When can I transfer these? Spore syringe to agar.

Pan Cambo Goliath



That one got poured a little thin, lol.

Pan Bisporus




The agar got a little chunky at the end there. Oops.

As you can see there are a buttload of spores there. Also, any advice would be greatly appreciated. This is my first pan grow and I am fairly new to agar.

Thanks.


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: TrKyNcKmSk] * 1
    #28498177 - 10/09/23 08:43 PM (3 months, 18 days ago)

Wait another 2 or 3 days before transferring. You want to transfer away from the spores, so let the cultures grow away from the inoculation sites. When you do transfer, take only the leading edge of the culture, and transfer as many different cultures as you can manage. Look for the fastest growing cultures, but don't be afraid to take anything that looks healthy. Fast cultures don't necessarily mean they will fruit well, but it's a good place to start.


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InvisibleMycomedicine888
Chief Executive Fruitcake
I'm a teapot


Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
Re: Official Copelandia/Panaeolus Thread [Re: TheAtlantean]
    #28498537 - 10/10/23 07:35 AM (3 months, 18 days ago)

Thanks!
Quote:

TheAtlantean said:
Beautiful flushes.



Thanks! Seems they like my setup :grin:


--------------------
I may have inhaled some of the spores....



Copelandia cyanescens 2023


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InvisibleMycomedicine888
Chief Executive Fruitcake
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Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente] * 2
    #28498543 - 10/10/23 07:46 AM (3 months, 18 days ago)

Quote:

Kunta Kente said:
Do any of you guys use a Martha setup? I'm interested in messing around with lions mane and oysters as well as pans, so I didn't know if that would be a reasonable idea or if you just do monotub like the 3.0 thread. I won't be able to exhaust out of the apartment. In a few months I'll have more room. Currently trying my first few pans in unmodded tubs.



Yeah i've grown Lion's Mane, Shiitake,all sorts of Oysters, a few different strains of Reishi, cubes and pans in bigger martha style setup (1,4x1,4m tents), it definitely gives me good yields and big fruits, but good cultures are half of the work i'd say...
They all require slightly different conditions though, so having one tent per species would be ideal and most oysters sporulate very heavy so def filter your exhaust going into your living space and regularly change the filters.
To stay on topic: the pans in the martha give an average yield 25g dry/4,5l of sub, which is 5 more than i got out of monotubs
Best of luck!


--------------------
I may have inhaled some of the spores....



Copelandia cyanescens 2023


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InvisibleTheAtlanteanS
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Registered: 06/25/18
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Re: Official Copelandia/Panaeolus Thread [Re: Mycomedicine888]
    #28498594 - 10/10/23 08:43 AM (3 months, 18 days ago)

Quote:

Mycomedicine888 said:
Quote:

Kunta Kente said:
Do any of you guys use a Martha setup? I'm interested in messing around with lions mane and oysters as well as pans, so I didn't know if that would be a reasonable idea or if you just do monotub like the 3.0 thread. I won't be able to exhaust out of the apartment. In a few months I'll have more room. Currently trying my first few pans in unmodded tubs.



Yeah i've grown Lion's Mane, Shiitake,all sorts of Oysters, a few different strains of Reishi, cubes and pans in bigger martha style setup (1,4x1,4m tents), it definitely gives me good yields and big fruits, but good cultures are half of the work i'd say...
They all require slightly different conditions though, so having one tent per species would be ideal and most oysters sporulate very heavy so def filter your exhaust going into your living space and regularly change the filters.
To stay on topic: the pans in the martha give an average yield 25g dry/4,5l of sub, which is 5 more than i got out of monotubs
Best of luck!





I noticed the containers you used….


I actually found those online a while back and purchased some and have them specifically for my pan grows.


What recipe did you follow and ratio did you choose for that size of container?

I was thinking about doing a half myco quart to each.


--------------------

Meditation is passive learning. Tabernacling with the one infinite Creator is passive learning. It is the learning from listening to the voice one cannot hear which answers questions which one cannot ask. The process cannot be known and cannot come into consciousness in local sense, yet the learning is there. And the strength of spirit and will which comes from this learning is helpful.
.

:egyptian:www.templeofhermes.org:egyptian:


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InvisibleMycomedicine888
Chief Executive Fruitcake
I'm a teapot


Registered: 07/10/21
Posts: 24
Loc: in between mushrooms
Re: Official Copelandia/Panaeolus Thread [Re: TheAtlantean] * 11
    #28498632 - 10/10/23 09:42 AM (3 months, 18 days ago)

A half myco quart to each sounds like chinese to me sorry :grin:

It's two700ml jars of spawn to +-4,5L of sub, that would make it almost 1/4.5 ratio
Spawn is rye with gypsum, finding good clean grain is harder than you would think, tried at least 7 different sources before finding the one i got now
Sub is composted cow manure (stable manure with lots of straw already in it)+verm(+gypsum) in a ratio fluctuating between 6/4 and 4/6,depending on how wet the shit is
Casing on this run was the old classic peat/verm w lime, first time i've done this
In the past i used cocopeat/verm, have to run more tests to make sure but i got better pinsets with that, it could be genetics too...
They can go for 5flushes without contam, my martha is setup in my living room so def not sterile. Airflow in and out is filtered though. As long as everything is super clean untill your sub is fully colonized they are quite robust to anything except sciarids for me, those really suck when they get in

As for the containers, they seem to work best when they're about 2inches taller than about 3inches of sub, but it does make for easier harvest when the sub is closer to the rim of the container.
My favourite are the shallower ones for easier picking, but best performance so far are these two:









Clear plastic one is12L, the black ones 9L, they had about 6-8L of sub if i remember correct...

Best of luck


--------------------
I may have inhaled some of the spores....



Copelandia cyanescens 2023


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