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Genome794
Spore-Whore-der & Agar-Addict


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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 1
#28438980 - 08/19/23 08:01 AM (5 months, 8 days ago) |
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Plus run a cube along side your Pans. Gift the cube everything you give the Pan.
If the cube bombs-out too, its your technique...
If only the Pans bomb-out, you can start adjusting what you do with the Pans.
After going back to spore several times... You might conclude that the culture is a dud.
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TechniQue1
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Re: Official Copelandia/Panaeolus Thread [Re: Genome794]
#28439041 - 08/19/23 09:13 AM (5 months, 8 days ago) |
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The first grow I ever did, got contamination. It was an all in one bag. Otherwise, the only failure I've had with cubes was the beginning of summer because temps indoors were too hard to control for them for the room that they were being grown in. So I stopped growing for a while and I'm about ready to start again. When I prep my wbs, I wash it and strain it until it almost looks like it's straight millet or Milo and the water comes out spotless. I don't leave it sit long enough to ferment, but I make sure it has enough time to hydrate and wake up any bacteria that may be lingering. Then, before my last rinse, I add peroxide to the grain, which works great because it will cause all of the corn and busted shells to float to the surface and it kills any bacteria prior to boiling it. Once I finish straining it again, it gets rinsed once more and then goes into a pot of water and heated, stirring occasionally until it boils, and then it comes off the stove and gets strained until it's barely damp, but not wet. Afterwards, it goes into jars with some gypsum and is pressure cooked in a name brand instapot for 2hrs. No flow hood, but I do have a sab... Though it's not used for grain prep, just agar and inoculation, if needed. I use gloves, a hat and n95s anytime I go into my grow room... As well as Lysol and clean clothing. I work in healthcare, so my sterile technique is usually better than most people's. But realistically, cheap wbs is for birds, so I highly doubt the FDA cares(not that they even care about our food if money is involved) if rats are living in it, pissing, or shitting in it. The only other grain I have this much of a problem with, is whole grain brown rice. Both of these grains seem to be an uphill battle. The last time I bought a bag of organic millet, I didn't have one failed jar. The last time I did rice, every jar failed, with the exception of a grain bag I made with a quart and a half in it.
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fatpup
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#28442383 - 08/22/23 04:47 AM (5 months, 5 days ago) |
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Quote:
Jakeoncid419 said:
Quote:
Scotay said: Honestly don't know the actual science behind it. I came across it in a fb group. I think that kinda stuff is amazing!! I sure am glad I have all of you around to bounce ideas/questions to. Always makes me smile when I see people getting this down! Happy hump day you filthy panheads!! šāļø
Alan Rockefeller Alan Rockefeller I am die are hosting another breeding/genetics class this coming November the exact date has not yet been set but will be posted by October 15 only 30 spots available per class we will cover everything from traditional hybridization of fungi, identifying a full clams, breaking dikaryotic cultures, back down into monokaryotic cultures Spheroplast & protoplast fusion, pcr sequencing, micro, and macro feature identification printing techniques and mushroom foraging and photography utilization of polyethylene glycol for hybridization and much more everyone. The signs up can list one other attendee as their reference each person will receive a 20% discount off of the total cost per referred person up to three referrals PM me for details. The class will be held on discord and is three hours long and includes live audio group call live video segments interactive video segments are open format, Q&A throughout the course and everyone who completes the class will be eligible to purchase spheroplast fusion reagent. And the cost is $300. Cents
That sounds like an awesome event! Way over my head in info but sounds fantastic. Thank you both.
Edited by fatpup (08/22/23 04:49 AM)
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kirkeng
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Re: Official Copelandia/Panaeolus Thread [Re: fatpup] 11
#28443080 - 08/22/23 05:52 PM (5 months, 4 days ago) |
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Managed to get a few fruits off the mystery SA. Not many but hopeful of finding something better down the line.

Had three tubs, two of them pinned but aborted immediately then remained stagnant for a month before I pitched them. This one pinned and somewhat matured, still hoping to see some caps open up and deposit but seems to have stalled somewhat. Pictured mushrooms are at about a week of growth. Took about two weeks after casing to get any pins.
The two tubs that aborted contained regular manure based substrate, but this one had the addition of a healthy amount of hwfp/sawdust added in. Iāll hopefully get some better development in later flushes but weāll see. Going to run a few other cultures as well to hopefully find something a bit better.
Running these in unmodded tubs and they appear to want more air as well so going to be partially removing the lid a bit more to see how that affects them, but glad to have gotten some kind of fruitations.
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BlindMyce
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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah] 2
#28443246 - 08/22/23 08:18 PM (5 months, 4 days ago) |
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Quote:
Bismillah said:
Quote:
Bismillah said: I got some pan cyan BVI on agar, wonder what you guys think. First transfer from swab plates. The black dishes are 1% ME, the crappy looking ones are GWA poured too cold.
Is it too early to say what to pick here? I guess the myc really likes the grain water. Is this growth too dense - should it be more wispy?

Going slowly with my BVI.. I waited a few days and put those yellow (GWA) plates to grain. The growth was around an inch in diameter. I also made some T2 plates, this time on a grain/potato mix agar, pics below. Couple of questions:
- The growth here seems much slower than what I see posted here. These T2 plates are 10 days old. Temps have been around 19-22C. - I get airial growth.. From what I understand this is not ideal. Should I just try another round of putting these in small grain jars?

Not sure if this makes me a contrarian but I feel pretty strongly about how to proceed from a spore print that i did not personally make. I believe it is best to get as much genetic diversity from the print first before trying to do much with agar transfers and any kind of isolation/selection. My approach is to immediately streak spores onto multiple plates, for the sole purpose of checking for contamination. I will use a few plates with gentamicin and chloramphenicol in case there's some insidious bacteria that is very motile (making it very difficult to clean by taking just the edges since it can reside in the same footprint as the mycelium). Once I'm confident it is clean, and without doing anything at all to isolate for genetics (in fact i make it a point to keep it as diverse as possible if I have to transfer), i spawn it to grain and grow. Only once there are fruits do I make an attempt at selecting for phenotypes and doing additional agar work. Great looking mycelium doesn't always translate to great fruits, it would be a shame to not only waste time isolating something that doesn't fruit so great but also discarding potentially great fruiting genetics in the process. My $0.02, but I know some others that I respect a lot that do it this way also.
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Bismillah
Scharf im Schlafanzug


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Re: Official Copelandia/Panaeolus Thread [Re: BlindMyce]
#28443404 - 08/23/23 03:35 AM (5 months, 4 days ago) |
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This is the approach I'm following, or I used water agar and a 0.05% MEA mix, not any antibacterial in the mix. The T1 plates went to grain. I made the T2 plates to see how the MEA (black plates) cultures would behave on GWA/PDA. This is my first pan cyan grow, just trying to learn as much as I can from the single swab I had.
That second last plate marked C2 stands out here: it's growing faster than the others and appears like mold to me.
Edited by Bismillah (08/23/23 03:39 AM)
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TechniQue1
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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah] 1
#28444292 - 08/23/23 07:03 PM (5 months, 3 days ago) |
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Attempt two... Pan cambos.
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Bismillah
Scharf im Schlafanzug


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Re: Official Copelandia/Panaeolus Thread [Re: TechniQue1]
#28444864 - 08/24/23 09:49 AM (5 months, 3 days ago) |
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I put some manure in a steam pot to pasteurize, but forgot to check that the max temp alarm was on. The core temperature went up to 85C before I noticed and switched it off. Any tip on how to proceed with this? I guess it was over 65C for at least 15-20 minutes.
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ppancho
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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah] 2
#28444934 - 08/24/23 10:33 AM (5 months, 3 days ago) |
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you can add some fresh manure and start the pasteurization again.
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psilocybist
All is One



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Re: Official Copelandia/Panaeolus Thread [Re: ppancho] 1
#28445090 - 08/24/23 01:06 PM (5 months, 3 days ago) |
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Nope. It's partially (but incompletely) sterilized. You should expose it to a wide range of bacterial flora, over a few days to be able to pasteurize it again.
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ruawakeyet

Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist] 3
#28445220 - 08/24/23 02:52 PM (5 months, 3 days ago) |
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If the fresh manure is mixed with the partially sterilized manure it will be fine to pasteurize again. The beneficials that remain will quickly colonize whatever got partially sterilized.
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astra
Mycelia-Of-House-Fungi


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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 2
#28445879 - 08/24/23 10:32 PM (5 months, 2 days ago) |
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Quote:
PsiloPsychIn said:
Quote:
ppancho said: Hello. Can I ask what FC are you using? is it a monotube?
very nice .
In this case they are in a fruiting set up like JCM's tek. A large tote with water in the bottom heated to 81-82F with an aquarium heater. There are also aquarium bubblers in the bottom to bring in fresh air and to contribute to humidity. The large tote has ventilation holes and also a computer fan that forces in fresh air and provides evaporation for 10 minutes per hour. Ive misted a bit when it looks like the fan is too drying. I have also fruited these in a mono tub and a Martha and even a roast chicken container at room temperature with success:



Couldn't agree more with your post I've been using the jcm FC with better results than my old grow tent while replacing my pyrex trays with costco food trays lol... so convenient and smooth 
Edited by astra (10/15/23 01:32 AM)
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Scotay
The Mad Mush Guy

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Re: Official Copelandia/Panaeolus Thread [Re: astra] 1
#28446763 - 08/25/23 07:08 PM (5 months, 1 day ago) |
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Happy Friday you filthy panheads you!! I hope y'all have had a decent week and I hope your weekend is filled with mushrooms up to your ears!! About to give this another shot. Fingers crossed. Now....Hurry up and wait!! šāļø
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! šāļø
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TheGoblin


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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] 2
#28446962 - 08/26/23 01:11 AM (5 months, 1 day ago) |
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So I finally decided to start my P.Cyan project about 4 days ago but swabbing spores directly onto a few agar plates, and holy shiet. P.Cyans are really aggressive on agar. I'm already seeing germination. It took twice as long for my cubes to germinate, aswell as P.Nataleasis.
Here they are:



Note: Apologies for the massive photos, my lazy ass can't be f'd resizing them. Sorry people.
Edited by TheGoblin (08/26/23 01:14 AM)
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THE MYCOSMITH
Weekend mycologist


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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] 1
#28446969 - 08/26/23 01:34 AM (5 months, 1 day ago) |
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:Quote:
kirkeng said: Managed to get a few fruits off the mystery SA. Not many but hopeful of finding something better down the line.

Had three tubs, two of them pinned but aborted immediately then remained stagnant for a month before I pitched them. This one pinned and somewhat matured, still hoping to see some caps open up and deposit but seems to have stalled somewhat. Pictured mushrooms are at about a week of growth. Took about two weeks after casing to get any pins.
The two tubs that aborted contained regular manure based substrate, but this one had the addition of a healthy amount of hwfp/sawdust added in. Iāll hopefully get some better development in later flushes but weāll see. Going to run a few other cultures as well to hopefully find something a bit better.
Running these in unmodded tubs and they appear to want more air as well so going to be partially removing the lid a bit more to see how that affects them, but glad to have gotten some kind of fruitations.
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A.k.a
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] 2
#28446979 - 08/26/23 02:47 AM (5 months, 1 day ago) |
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Oh man if those took a week to grow and still arenāt done Iād be pretty terrified to try them out 
I like the way they look the caps have a unique thing going on.
--------------------
LAGM2020     
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BlindMyce
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a] 1
#28447105 - 08/26/23 07:54 AM (5 months, 1 day ago) |
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Firstly, i regret cross posting the following which i posted as a separate thread, I didn't think to post in this big Pan thread at the time. Not sure how to delete the topic I started. Looking for a little help here.
So i grew another batch of Pan Cambodginiensis Sandose domesticate from the same LC that I've used before. Everything went just like the first time i grew these until what I would call the second flush of pins started to come in. They had brown/darker looking caps. At first I thought it must be just from spores dropping on them but at this point I don't know anymore. 50% of the brown/dark capped ones in the picture were not even pins yet when i did my first harvest, so I don't see how spores could be the reason. Any ideas what could be going on? In this picture you can see some late bloomers (the white caps) of what I'm calling the first flush as well as these brown/dark capped ones that came in at the tail end of the first flush. Even the stipes are a different shade.
I did not observe this the first time I tried growing these from the same LC a few months ago. I'm pretty sure i got two flushes out of the first batch (tossed it after two harvests to make room for other things so I suppose it is possible that's why I didn't see this but given these things pretty much overlap some between flushes i'm not very satisfied with this explanation).
I don't know why but for some reason it feels concerning to me.

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kirkeng
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Re: Official Copelandia/Panaeolus Thread [Re: BlindMyce]
#28447235 - 08/26/23 10:15 AM (5 months, 1 day ago) |
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@blind, those dark colored caps look a lot like tamps to me, the stem and dark caps with the slight white ring at the edge make me think tamps. Perhaps some contamination? Are you growing tamps as well?
The caps on the mystery SA did open up a tad but wonāt drop spores, the rest of the pins have stalled out and refuse to continue growing⦠harvested what was āmatureā. Thinking Iāll clean the rest of the pins and try for a second flush. Got another culture brewing for a second attempt though.
@AKA, yeah theyāre pretty, what got me though was the smell.. I think bisporus smells like anise when fresh and these have a hint of that smell but with a bit of fishy grass mixed in, they smell like if I had a handful of fresh bisporus and fresh cyans. Very odd.
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Timestop413
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] 5
#28449748 - 08/28/23 10:52 AM (4 months, 30 days ago) |
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Has anyone had the issue with their Pans growing really short on flushes after the first? This is with TTBVI on a second flush but I also experienced it with Estero. Is this normal or no? Any idea what might cause it if its not normal?
Edited by Timestop413 (08/28/23 10:53 AM)
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RoscoeReturns
Crotchety chode man



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Re: Official Copelandia/Panaeolus Thread [Re: Timestop413] 7
#28449873 - 08/28/23 01:00 PM (4 months, 30 days ago) |
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Pan cyan Nec D. This is a clone culture from the tiniest of fruit from a pint jar Muda style. I cloned it because it was the first time I got Pans to fruit from spore. Now Iām getting sporadic pinsets in a tray with a few of these guys. How big do Pans get?

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