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0t0lerance


Registered: 05/01/21
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#28356759 - 06/12/23 09:17 AM (7 months, 14 days ago) |
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O man.. al those bisporus.. I still don't see pins after a week
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freqfas
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Re: Official Copelandia/Panaeolus Thread [Re: 0t0lerance]
#28356992 - 06/12/23 11:51 AM (7 months, 14 days ago) |
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Hey all, so I'm currently in the process of isolating from a ms germination for my first attempt with pans (BVI). I wanted to check whether what I'm seeing is normal.
So I've got a couple of agar (MEA and activated charcoal) in little 2oz portion cups, containing t1 cuts. These were transferred maybe 11 days ago.
So yesterday, (a few days late on taking t2 cuts) I check them and realize 2 of the t1 dishes them have pins growing. Found a third this morning too. Of these, one has a straight up cluster popping out of some nice wispy mycelium climbing the cup side. I've seen pins on agar with cubes, but only a couple times after forgetting a dish for a few weeks too long. Never this fast tho, and never a cluster of them
So the first question, has anyone else had pans pin on agar? Would you consider this a desirable trait, or problematic?
Next, what should I do with the dishes in question? They've got a nice mix of wispy and fluffy mycelium, but a few appear almost ryzomorphic which I've read isn't super common for pans. Should I just transfer a few sections of the myc and leave the pins for experiment sake?
Or should I break out a jewelers loop, and try to take a tiny tissue sample from a fruit the same diameter of an 18 gauge needle?
I'll attach pics in a little bit once my coffee starts working well enough to go back upstairs to snap a few more.
Edited by freqfas (06/12/23 12:22 PM)
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freqfas
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Re: Official Copelandia/Panaeolus Thread [Re: freqfas] 3
#28357019 - 06/12/23 12:12 PM (7 months, 14 days ago) |
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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: freqfas] 1
#28357165 - 06/12/23 02:23 PM (7 months, 14 days ago) |
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You can transfer a whole plate pin to a new dish, no need for dissecting it as it is a in vitro fruit and sterile. Take a few of these pins to new plates. Selecting clones and transfers is always a good deal of experimentation and if you want to find something real good it does require a bit of searching and trying. Having said this, at the same time I would avoid going over capacity and ending up with too much stuff.
What agar recipe are you using?
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Genome794
Spore-Whore-der & Agar-Addict


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Re: Official Copelandia/Panaeolus Thread [Re: pesa] 2
#28357265 - 06/12/23 03:38 PM (7 months, 14 days ago) |
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Quote:
pesa said: its not airborne its bit like your jar dust thingy
edit: upon more closer look it looks like dry mycelium, like you leave bunch of plates for months and forget about them and they go all dry and dusty, its that dusty thingy
Sorry to backtrack so far...
I gave this dust/powder/dried myc some thought and "the devil is in the detail"
So these are what I think are better jars

And these are "powdery"

The good jars and older than the powdery jars. Bump the powdery jar and the grains come loose way too easily. The good jars do nothing, the myc holds it all together...
In my thinking, if I dont know the age of the jar, anything powdery "should" be considered as contaminated. Why do I think that?
Well, because so far - for me - that has been true. Any good jar that I have left for any extended period of time has not made "dust" when being handled. In fact the opposite... The contents of the jar stiffens and becomes more difficult to breakup. I suppose at a point it could happen that the contents of a good jar drys enough to become brittle but that still wont cause "dust" on minimal handling - unless it was broken up before and after being very dry/desicated.
I know, somewhere in How it should & shouldn't look - NEW CULTIVATORS GUIDE it says that hard to breakup mycelium in a jar is a bad sign... Thats cubes though and try tell that to ATL7 thats been standing a week or two too long.
Last, if green dust, black dust and brown dust in your jars is bad then white dust cant be good either. Or do I need to go eat a fist full of Shrooms...
Let me know if I have it wrong (and where). Thanks 794
Edit: If the powder in my jar looks like icing sugar/confectioner's sugar... It ain't myc. Look again at the powdery jar. It's like I dusted the grains with super fine sugar.
Edited by Genome794 (06/12/23 03:41 PM)
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Genome794
Spore-Whore-der & Agar-Addict


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Re: Official Copelandia/Panaeolus Thread [Re: Genome794] 2
#28357290 - 06/12/23 03:53 PM (7 months, 14 days ago) |
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Alright... Let me try make this a shorter update.
From my last big run on plates from PCN3 (Pan Cyan Natal - round 3). Bad plates
     All dumped - maybe 15 of them.
Good plates
   I think there are 12.
Top fruiting these jars - From PCN2. They got a (1:1 - 50/50+:sand) casing two days ago.

These are some mini-jars that are older than the jars above (and up to three weeks older than the powdery jars) - I will use them for G2G soon.

Lastly, PCN4 (the start of Round 4) germ plate (sorghum, oats, BRF and cow dung).
 You can see I have taken myc from the two fast patches. I am hoping not to see powder here.
If anything looks off let me know. Thanks 794
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YoshiTrainer
Onion tied to belt



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Re: Official Copelandia/Panaeolus Thread [Re: Genome794] 2
#28357327 - 06/12/23 04:10 PM (7 months, 14 days ago) |
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This is for your previous post.
I'd suggest, the bottom ones are definitely funky! Powdery anything or color in your jar is bad! I'd also guess your top jars have wild yeast at the very least going on. The little white dots stuck to the inside of the jar usually is a sign.
In my opinion, stalled agar is dirty still. Healthy pan mycelium tends to move pretty quick. I tend to look at things in 2 week intervals. Once clean, you should be able to A2G, 2 weeks later G2G or G2B, 2 weeks later case and put your bulk to FC. Certainly there are variables but if I've shaken a grain jar and it recovers slowly or takes 3 weeks to finish, I'm very suspicious!
Make sure your agar is clean, though learning to ID funk can be valuable too.
Good luck!
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ekabpils
Exploring Exotics



Registered: 10/20/21
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 6
#28357864 - 06/13/23 12:59 AM (7 months, 13 days ago) |
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Amazing Senguio:
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rockyfungus
dirty


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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
#28357901 - 06/13/23 03:36 AM (7 months, 13 days ago) |
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I've made some transfers of my "stones" and it looks to be healthy recovery.
My work area fluctuates from the 50s to mid 60s and I noticed in the colder months everything stalls and stones. After a few years of this and wasting multiple prints, I think it's normal across pans?!
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Rush56
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus] 2
#28357908 - 06/13/23 03:59 AM (7 months, 13 days ago) |
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What do you guys think on using a compost, from what i can tell is a mixture of cow and horse poo. Or maybe just horse poo, since owner does not have cows i think There is a big pile close to me, its been a year or two old for sure.
A lot of different mushies grow from it, including some large panaeolus speciments. Not cyans though 
From observing how abundantly these grow there, am i good using that for substrate?
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Smoothcat
Renegade-master



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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
#28358135 - 06/13/23 09:11 AM (7 months, 13 days ago) |
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Quote:
ekabpils said: Amazing Senguio:

Very nice 
Whats this setup with the water in the middle all about?
-------------------- Back once again with the ill behaviour Links I like
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freqfas
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28358560 - 06/13/23 02:57 PM (7 months, 13 days ago) |
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I forget the exact proportions, but I've been using mea + activated charcoal + peptone. I moved a couple pins to agar along with cuts from regular mycelium last night. Using wbs for spawn, then will probably spawn to poo+coir with peat casing.
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Genome794
Spore-Whore-der & Agar-Addict


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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils] 1
#28358575 - 06/13/23 03:10 PM (7 months, 13 days ago) |
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Quote:
ekabpils said: Amazing Senguio:

I want boxes like that too. Well done man. I am very jealous - in the good way.
Update From the good plates in my previous post. I took small wedges to sorghum+cow dung(5%) spawn. The donor plates are still clear - no pwodery stuff or changes in Mycelium. 3 Examples of what the growth looks like.
   The transfers were done on June 8. Average grow area temp is 17-18C. Last two days a cold front came through bringing it down to 14C. Far from ideal growing temps but at least it is looking promising.
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csilocybepubensis
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Re: Official Copelandia/Panaeolus Thread [Re: Genome794] 6
#28358601 - 06/13/23 03:37 PM (7 months, 13 days ago) |
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Got the pans finally doing something. Ttbvi t1 to wbs. Spawned to 20 percent manure 80 percent coco. Cased at full Colo. Fruiting outside in Florida. The fogger comes on 10 minutes every 50 mins. We are 5 days into casing and putting directly to fruiting.
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fatpup
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How are you fruiting them?......I have a few trays of pan Peace River and trying to find easiest/best way to try and fruit. One tray is almost ready to case. Also in Fl. You go open air and fog?
I used to find a ton on wild pans....but only found them Sept-early nov.
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SGV
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Re: Official Copelandia/Panaeolus Thread [Re: fatpup]
#28358873 - 06/13/23 07:46 PM (7 months, 12 days ago) |
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Just poured a handful of LC jars from (my favored) JP method.
Strangely enough, my Estero LC grew ridiculously faster than this latest Tampenensis LC I did at the same time. Should see some aggressive growth in the oat jars and hopefully they don't trich or stall.
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csilocybepubensis
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Re: Official Copelandia/Panaeolus Thread [Re: fatpup]
#28358891 - 06/13/23 08:01 PM (7 months, 12 days ago) |
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Yeah man for now this is what I'm doing. That tub catches the fog and creates a nice climate for them apparently. Tried a few things and this had primordia from day 3 after casing on two tray,both ttbvi. I have been watching it every few hours to check the moisture level and adjusting as needed. Been running the fogger for 10 mins every 60 mins now and it is set pretty low maybe 3/10 on the knob. I was saturating the casing extremely heavy running at 30 mins on 60 off. It seems now it's dialed in pretty good at least for the night. I'm sure when it gets 95 degrees tomorow I will have to kick up the humidifier to half power or so. Just doing this while I'm setting up a proper tent indoors. Good luck man Edit....also watch how much sun you allow them to get because it will fry them at these temps recently, been pushing upper 90s.
Edited by csilocybepubensis (06/13/23 08:02 PM)
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ekabpils
Exploring Exotics



Registered: 10/20/21
Posts: 167
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Re: Official Copelandia/Panaeolus Thread [Re: Smoothcat]
#28359083 - 06/13/23 11:03 PM (7 months, 12 days ago) |
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Quote:
Smoothcat said:
Quote:
ekabpils said: Amazing Senguio:

Very nice 
Whats this setup with the water in the middle all about?
I place aquarium compressor therer for oxygen and moisture
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Smoothcat
Renegade-master



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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
#28359312 - 06/14/23 08:25 AM (7 months, 12 days ago) |
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ahh ok so is it just the bubbler or do you have a heater running as well in there?
Im almost ready to spawn some pans and I have all the stuff for a jcm style fc but always looking at other ways to do things
-------------------- Back once again with the ill behaviour Links I like
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AspectOfTheCreator
Mastering the Art of Success


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Re: Official Copelandia/Panaeolus Thread [Re: Smoothcat] 1
#28359371 - 06/14/23 09:41 AM (7 months, 12 days ago) |
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Plain grain jars all stalled close to completion. I shook some and they dont seem to be recovering.

The bvi grain/manure jars seem to have completed in 2 weeks with just one shake. Will be spawning these to 3 qts cvg with a top layer in shoeboxes. No manure in top layer. This will give a 1:4 ratio with 25% manure cvg.

The pan cambo grain/manure jars are dead. I think I used a poor culture. Started 2 more with my best culture. Along with 2 more bvi.
Overall very frustrating and im beginning to feel the juice is not worth the squeeze. All the wasted materials are just not worth it. All this species seems to want to do is stall. The jars have been at a healthy 75 F since summer is rolling in. Everything was seemingly perfect. The growth starts off very strong and healthy. Never any sign of contam. Yet still they fail.
Edited by AspectOfTheCreator (06/14/23 10:19 AM)
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