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Invisiblestagger
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Registered: 05/05/19
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Re: Official Copelandia/Panaeolus Thread [Re: ppancho]
    #28327187 - 05/20/23 06:36 AM (8 months, 6 days ago)

Quote:

ppancho said:
Hello everyone.
What would you choose from this plate? This is T1.








If it were me, I would transfer from the colony at the bottom, or 6 o'clock position...I would probably take the cuts from the 1 o'clock to almost 3 o'clock areas from this colony.


Edited by stagger (05/20/23 06:37 AM)


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Invisiblestagger
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Re: Official Copelandia/Panaeolus Thread [Re: stagger]
    #28327222 - 05/20/23 07:14 AM (8 months, 6 days ago)

But really there seems to be a number of areas to cut from there.  I can't offer an in depth analysis but if you have the plates, time and space than consider cutting from multiple colonies and watch/grow it out.


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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: ppancho]
    #28327306 - 05/20/23 08:42 AM (8 months, 6 days ago)

Quote:

ppancho said:
Hello everyone.
What would you choose from this plate? This is T1.








9 o'clock from the top left myc.
And that pointy 11 o'clock from the top right one.


Edited by psilocybist (05/20/23 08:43 AM)


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Offlineppancho
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Re: Official Copelandia/Panaeolus Thread [Re: stagger]
    #28327399 - 05/20/23 10:04 AM (8 months, 6 days ago)

Hello

thanks for the advice.
yes, exactly from the bottom 1 to 3 hours I took.
i ended up doing 8 transfers. so we'll see.


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OfflineAspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: ppancho] * 1
    #28327477 - 05/20/23 11:22 AM (8 months, 6 days ago)

Results are in:

Not sure if it was the hpoo, the grain water, or not cling wrapping the plates but if I had to make a guess, it would be the grain water. Cultures are no longer stalling.



Unfortunately that was the only culture that grew out nice and symmetrically like that. My bvi plates look clean but mostly all grow like this



Im thinking maybe the hpoo made the nutrient levels not uniform across the plate. Going to try without hpoo next time.

Regardless, put the PC plate to 2 jars and the best growth of the BVI plate to 1 jar.

Not going to say pan myc wont grow on LMA because clearly people are doing it and making it work. But in my experience they all stall out every time. First try with GWA and had success. No plates stalled.


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OfflineGenome794
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Re: Official Copelandia/Panaeolus Thread [Re: AspectOfTheCreator]
    #28327616 - 05/20/23 02:01 PM (8 months, 6 days ago)

Hey Aspect,

Looking good. Glad to hear you are finding answers/solutions.

I did a small batch of Sorghum jars with cow-poo to get some grain water. 50/50 dilution. The plates that look like they dont have the "powdery contam" will go to 10 new plates tomorrow.

An update from my side.
The jar that got the colony that had the "powdery contam" now looks like this - 5 days from transfer.

other side


And next to the two jars that received agar pieces from different plates the same day

The jar that got the suspect agar seems to be growing at the speeds I have read about. My concern is that it is quite cold here now. My grow area averages 17C by day. Unless the cow-dung in with the grain has that effect.

Again, any comment welcome.

(When I have freed up some plates, I will do a small batch of water agar.)


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Invisibleekabpils
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Re: Official Copelandia/Panaeolus Thread [Re: Genome794] * 10
    #28328186 - 05/20/23 11:19 PM (8 months, 5 days ago)

Finally a little bit of PHV:


--------------------


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OfflineYoshiTrainer
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
    #28328190 - 05/20/23 11:32 PM (8 months, 5 days ago)

Nice job Ekabpils are you using tent or tub?


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OfflineAspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
    #28328379 - 05/21/23 06:27 AM (8 months, 5 days ago)

Pan bvi jar stalled right before completion



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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: AspectOfTheCreator] * 1
    #28328523 - 05/21/23 09:10 AM (8 months, 5 days ago)

Quote:

ekabpils said:
Finally a little bit of PHV:





Beautiful!!!!! I'm glad that print worked out! Have any problems getting it cleaned up?


Quote:

AspectOfTheCreator said:
Pan bvi jar stalled right before completion






I typically use WBS, and I have noticed that the millet will often times look like that. I think it is colonized, but due to the really hard shell, it looks uncolonized. I've always sent spawn to bulk when it looks like that, and have had decent success. (I've had some contamination before, like anyone, but I don't think it's because of the millet.)


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OfflineAspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #28328548 - 05/21/23 09:23 AM (8 months, 5 days ago)

Hmm. Thanks for the reply. I shook it up to see if it would go further. Ill try spawning when i get time monday or tuesday


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Invisibleekabpils
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #28328639 - 05/21/23 11:05 AM (8 months, 5 days ago)

Quote:

ekabpils said:
Finally a little bit of PHV:





Beautiful!!!!! I'm glad that print worked out! Have any problems getting it cleaned up?






Thanks a lot for the print. No problem with cleaning it up - I use agar from the beginning


--------------------


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Invisibleekabpils
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] * 2
    #28328642 - 05/21/23 11:06 AM (8 months, 5 days ago)

Quote:

YoshiTrainer said:
Nice job Ekabpils are you using tent or tub?



Hi! I use someting like this:


--------------------


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InvisibleTheDoobskerS
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
    #28328982 - 05/21/23 04:07 PM (8 months, 4 days ago)

Quote:

ekabpils said:
Finally a little bit of PHV:





:bow2:


--------------------


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Onlinesonson
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Re: Official Copelandia/Panaeolus Thread [Re: TheDoobsker]
    #28329198 - 05/21/23 08:08 PM (8 months, 4 days ago)

Some helps from experienced Panaeolus Bisporus growers are appreciated.

Agar plates will be ready soon and need to prepare some bulk grain and fruiting substrate.

I happen to have a good amount of Douglas fir sawdust pallets and straws and want to try both and compare.

I've read some threads and made a plan:

- Use grain:sawdust = 7:3. PC 90min @ 15psi
- Manure:sawdust/straw:verm = 41:41:18. Pasteurization 12hrs @ 72-75 Celsius, wait for five days.
- (Casing layer) Peat moss:verm = 10:10(+ 1 gypsum). Same pasteurization.

Is it good to go?

Thanks!


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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OfflineScotay
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Re: Official Copelandia/Panaeolus Thread [Re: sonson] * 2
    #28329341 - 05/21/23 10:52 PM (8 months, 4 days ago)

Hey there Sonson! Good luck with everything you got goin Growmie!!!
I hope everybody has had a good weekend and all you panheads are up to your ears in mush!!!
I started a jar with some agar that looked good. Since my luck hasn't been great I decided to do agar at the same time I was gonna do the grain.
I streaked 10 plates and dropped agar to the grain jar. Hurry up and wait folks!! 😎✌️




--------------------
Just chillin like a Villain and talking to like minded individuals!!
             


              Mush Love!!
                😎✌️


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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] * 1
    #28329447 - 05/22/23 02:05 AM (8 months, 4 days ago)

Your "hurry up and wait" motto gets me every single time.
:sun::sun::goodluck::sun::sun:


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OfflineTimestop413
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
    #28329886 - 05/22/23 12:07 PM (8 months, 4 days ago)

Hey I have a question for anyone who is growing or has grown Pan Bisporus, I often see these mushrooms with the caps far opened and sometimes inverted.  Is it ok to let them grow to this point or will I be losing potency if I do so?

Basically..when Is ideal time to harvest this species? thank you.


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OfflineAspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Timestop413]
    #28329972 - 05/22/23 01:08 PM (8 months, 4 days ago)

Hard to tell but my BVI jar that I suspected was stalled is recovering nicely after a shake. If it rebounds completely Im planning on spawning it wednesday to manure/cvg via liquid pasteurization tek.



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OnlineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: sonson] * 1
    #28330051 - 05/22/23 02:22 PM (8 months, 4 days ago)

Quote:

sonson said:
Some helps from experienced Panaeolus Bisporus growers are appreciated.

Agar plates will be ready soon and need to prepare some bulk grain and fruiting substrate.

I happen to have a good amount of Douglas fir sawdust pallets and straws and want to try both and compare.

I've read some threads and made a plan:

- Use grain:sawdust = 7:3. PC 90min @ 15psi
- Manure:sawdust/straw:verm = 41:41:18. Pasteurization 12hrs @ 72-75 Celsius, wait for five days.
- (Casing layer) Peat moss:verm = 10:10(+ 1 gypsum). Same pasteurization.

Is it good to go?

Thanks!




Should be good. Bisporus isn't too picky. Mine even colonized (pure) Spruce shavings after spawning some to it, although slowly due to their very low density.

Quote:

Timestop413 said:
Hey I have a question for anyone who is growing or has grown Pan Bisporus, I often see these mushrooms with the caps far opened and sometimes inverted.  Is it ok to let them grow to this point or will I be losing potency if I do so?

Basically..when Is ideal time to harvest this species? thank you.




I used to pick them before inverting, now I let them do their thing. Potency isn't an issue - just work on your technique and soon you'll have more than you could ever consume.


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