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OfflineGuystokesau
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay]
    #28316621 - 05/12/23 01:12 AM (8 months, 14 days ago)

I had oats do this for AGES, what I found worked for me was soaking them in warm water overnight and then I did my boil - simmer - dry - pressure cooker.

I think oats just have spores in the cracks and I had to germinate them before pressure cooking, maybe add another hour to your cycle. 

Saying that, change one thing at a time to work out where you are going wrong with the sterile tek, if you can nail down what you are doing wrong it will save you so much more time in the long run then changing your whole process (like I used to do every time)

Lol, I can't be the only one that tried 50 different grains before I actually  started showering and wearing a mask before the air box.


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Offline0t0lerance
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Re: Official Copelandia/Panaeolus Thread [Re: Guystokesau] * 1
    #28316678 - 05/12/23 02:40 AM (8 months, 14 days ago)

Quote:

Guystokesau said:
I had oats do this for AGES, what I found worked for me was soaking them in warm water overnight and then I did my boil - simmer - dry - pressure cooker.

I think oats just have spores in the cracks and I had to germinate them before pressure cooking, maybe add another hour to your cycle. 

Saying that, change one thing at a time to work out where you are going wrong with the sterile tek, if you can nail down what you are doing wrong it will save you so much more time in the long run then changing your whole process (like I used to do every time)

Lol, I can't be the only one that tried 50 different grains before I actually  started showering and wearing a mask before the air box.





You can save 1 jar of grain.. leave it untouched while you process the rest, if everything contaminates but your control jar.. you need to work on sterile techniques.. however, sometimes you accidently move too quickly.. and that can also ruin everything.. it happens


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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] * 2
    #28316966 - 05/12/23 08:40 AM (8 months, 14 days ago)

Quote:

Scotay said:
Fucckkkk....... attack of the green monster....
AGAIN.... I'm thinking maybe I should try a different kind of pan because I'm either majorly screwing up in my sterile technique or the oats are hit. It looked good on agar. This shit came on very quickly. Aggravated with it.
It's still nice seeing/hearing everybody's experiences!! I'm gonna try a different kind of print.  Happy Friday panheads.
I wanna be a panhead too damnit!! 💯🤣😂







How this jar got inoculated?
"It was clean on agar" you say, but the green being scattered all over the grains, it seems it's inoculated by some liquid (LC/LI or MS directly?)
Or you've shaken the jar after some colonization?

If PCed long enough, in correct PSI, no spores/endospore can survive, no matter what grain, pre-soaked or not, being cracked as oats or smooth as corn. So as 0T0lerance suggested, leave a jar as control and see if they're actually sterilized.
BTW, your jar lids are not shown, if not equipped with a gas exchange port, maybe you've tighten them right after PC. This will surely lead to contam when the jar is opened. (my mistake with big batches of jars back then, sterilizing back to back in a rush, wasting time/energy/hope/grains).
You should let PC/jars cool down completely before tightening the lids.


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist] * 1
    #28317006 - 05/12/23 09:16 AM (8 months, 14 days ago)

:whathesaid:

Especially since the mold is Penicillium, which is not known to have thermally stable spores any more than other fungi. It's almost certainly an inoculation procedure error. And 100% not a sterilization error (aside from keeping unmod lids sealed shut).


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OfflineMeaux Da Fun Guy
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay]
    #28317058 - 05/12/23 09:57 AM (8 months, 14 days ago)

Greetings Scotay

  I agree with the rest of the replies in saying to use a control jar but I’m gonna suggest a step further and say also do a agar transfer as another kinda of control.  If your agar transfer or non inoculated control jar don't contaminate then it’s your sterile technique… if the agar tams then your Harboring contaminants.  if the control jar tams your probably not pcing long enough.
      Sorry for loss Scotay it happens to the best of us.


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OfflineScotay
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
    #28317139 - 05/12/23 11:46 AM (8 months, 14 days ago)

Hey psilo! Thanks for the input. I actually used agar for the grain. I'm figuring it's something to do with my technique as well. I appreciate you being straight up. I just took a picture of one of the agar chunks. (Don't mind the color of the agar. I used green food coloring!! Foretelling maybe!?!?🤣😂) Happy Friday to you panheads all over!!
😎✌️

https://files.shroomery.org/files/23-19/391349904-IMG_20230512_133738930.jpg


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OnlineAspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay]
    #28317230 - 05/12/23 01:32 PM (8 months, 14 days ago)

Promised an update here it is:

Been having trouble for past 6 months trying to grow pans. All my cultures stall on agar then get a powdery look to them. Using 0.5-1% LMEA. Changed to grain water/hpoo agar a week ago. As per someone's suggestion, I also left plates unwrapped (was using glad wrap previously) and in a clean shoebox. Just taped them closed with micropore tape. Wanted to change things up because absolutely nothing was working after 6 months.



Only been a week but so far the myc looks more like what I see from other people. For the first time I can see that fine leading edge myc Ive learned to associate with clean growth with cubes. Its also growing more symmetrically. Was never getting that before. I think next time maybe I should dilute the grain water a bit just for a little lighter growth. Eager to see what I get out of the germ plate.

Heres the stalled cultures on LMEA.



They stall for a week or so then start growing a bit with very weird unhealthy looking myc afterwards.

Will update again in a week or so. Praying to the mushroom god that the myc doesnt stall.


Edited by AspectOfTheCreator (05/12/23 01:47 PM)


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Offlinepesa
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #28317425 - 05/12/23 04:14 PM (8 months, 14 days ago)

i got a germ plate of pan cam, can i put it in refrigerator for later use like we do with cubes? or pan mycelium die at lower temperatures?


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OfflineScotay
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Re: Official Copelandia/Panaeolus Thread [Re: Mr Piggy] * 1
    #28317911 - 05/13/23 01:15 AM (8 months, 13 days ago)

I just hit the jackpot on very nice aged manure piles from a stable my friends are involved with so I'm happy. I also grow a really nice urban garden. Outside.... I plan on putting all my spent blocks and crumble them into my regular bags that I grow in. I'm gonna have some killer tomatoes and very hot peppers...  I'm definitely gonna get several loads to mix with my already organic soil.
I'm still frustrated over the jars going fuck all crazy. I started 6 new plates though and streaked those.
Hurry up and wait!!! Happy Saturday panheads!! Send me some of your good mojo!!! 😎✌️


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Just chillin like a Villain and talking to like minded individuals!!
             


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Offlinerockyfungus
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Re: Official Copelandia/Panaeolus Thread [Re: pesa] * 1
    #28318073 - 05/13/23 07:26 AM (8 months, 13 days ago)

In my experience they do weird things in the fridge and don't last as long as my typical cultures.
I also tend to get a ton of blue bumps lately from any cold. I don't think cold is a useful thing for these.

Last winter pans never filled out plates as my lab is in the 50s. 3 diff varieties "stoned" from the cold. Or I got some really nasty tag a long just going for pans.


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OnlineThe Tao
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus] * 2
    #28318100 - 05/13/23 07:57 AM (8 months, 13 days ago)

First try with these. BVI brf puck cased with coir. Keeping an eye on the whiter growth, hoping it’s just weird mycelium.


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OfflineTimestop413
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Re: Official Copelandia/Panaeolus Thread [Re: AspectOfTheCreator] * 1
    #28318435 - 05/13/23 03:27 PM (8 months, 13 days ago)

for what its worth, I've sent stalled plates to grain and they can take off again and colonize fine/healthy.  I'm not sure what the reason is for the stalling but it happens with pans.  JOC has mentioned this in his thread as well.

Could be contamination but I dont know.  Have you ever put any of your stalled plates to grain?


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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: Timestop413]
    #28318453 - 05/13/23 03:47 PM (8 months, 13 days ago)

Quote:

Timestop413 said:
for what its worth, I've sent stalled plates to grain and they can take off again and colonize fine/healthy.  I'm not sure what the reason is for the stalling but it happens with pans.  JOC has mentioned this in his thread as well.

Could be contamination but I dont know.  Have you ever put any of your stalled plates to grain?




I did, it colonized to around %20 very healthy and quickly and stalled, not even shaken. Just stopped growing. This happened to all my 12 jars. (Which were not bacterial afaict, no stink, no watery shit, no fishy colors)
But same plates transferred to LC colonized fine, and that LC to grain stalled again.
I gave up on the batch as a whole, and retry after or during summer.
Right now I'm just busy with semperviva and a few varieties of cubensis , which are very promising in growth and fruiting.

Won't waste my time/grains on pancyan anytime soon, but when I do later, I'll surely commit all my attention/plates/setup to this species, and will succeed. I know it's worth it. But can't grow it as a side quest now.


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OnlineAspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Timestop413]
    #28318530 - 05/13/23 05:10 PM (8 months, 12 days ago)

Ive just recently began putting cultures to grain again. In the past the jars would never fully colonize. I put a tiny wedge of promising growth from a BVI germ plate (unwise, I know) to grains. I shook after a week and it looks very promising so far. I also inoculated a quart jar with a crumb of Pan Cambo on pf tek. Going to put the new cultures to the next round of grain jars in about a week. Also going to try crumbling some cambo brf pucks to hpoo/coir. Will definitely be posting results here.


Edited by AspectOfTheCreator (05/13/23 05:42 PM)


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Offlinepesa
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
    #28318545 - 05/13/23 05:27 PM (8 months, 12 days ago)

great so its always start from spores every season that's a bummer, i just got a clean culture dont know how to store it.


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OfflineTimestop413
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Re: Official Copelandia/Panaeolus Thread [Re: pesa]
    #28318568 - 05/13/23 05:47 PM (8 months, 12 days ago)

Quote:

pesa said:
great so its always start from spores every season that's a bummer, i just got a clean culture dont know how to store it.




in the few cultures I've grown of pans, starting from spore has been very fast and generally has produced good results.  Is there a lot of evidence supporting that clones are better with this species?


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OfflineYoshiTrainer
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Re: Official Copelandia/Panaeolus Thread [Re: pesa] * 2
    #28318620 - 05/13/23 06:36 PM (8 months, 12 days ago)

Quote:

pesa said:
great so its always start from spores every season that's a bummer, i just got a clean culture dont know how to store it.




Dried grains or mycelium in distilled water work pretty well for storage.


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Offlinepesa
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
    #28319129 - 05/14/23 06:18 AM (8 months, 12 days ago)

i don't know the heck is wrong with spores i got,they wont germinate even after month, lucky i got pan cambo germinated and i got it clean, good idea.
i am gona attempt to store clean mycelium in distilled water


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InvisibleTheDoobskerS
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Re: Official Copelandia/Panaeolus Thread [Re: pesa] * 4
    #28319331 - 05/14/23 09:45 AM (8 months, 12 days ago)

First flush of some Nec D.
Got 5 prints to give out, lmk if anyone is interested :thumbup:



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OfflineBirdsOnTrees
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Re: Official Copelandia/Panaeolus Thread [Re: TheDoobsker] * 1
    #28319368 - 05/14/23 10:19 AM (8 months, 12 days ago)

Hey guys, after struggling with stalled and contaminated pan cam plates for a while I've been making some progress. I had one stalled plate I threw in some karo water to make LC, which showed notable growth, which I then inoculated some rye berries with. It seemed to catch and has been growing for the past 3 weeks. Around 30% I shook it, like 4 or 5 days ago and it's looking as such now:





It seemed to recover from the shaking but then stalled a bit. I'm tentatively hopeful things are going well.

I was also able to finally get some really nice pan cam plates. Previously it has just been thick marshmello-y mycelium which grows up and off the plate in blobs, but around T5 I finally got some nicer somewhat rhizo growth that quickly spreads:







I'm going to send these to grain once I send the cube grain jars to bulk in the next couple days. Wish me luck friends!


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