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0t0lerance


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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 1
#28283072 - 04/18/23 02:33 AM (9 months, 7 days ago) |
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Quote:
PsiloPsychIn said: Hey Folks I bought a Pan Bisporus (Coville WA) spore syringe from a reputable vendor and the date on it is 10/22. There is plenty of dark spore looking stuff in the syringe. To my surprise the first attempt at germinating (2.5 weeks now) there has not been a wiff of germination of any thing. Other spores from prints that I inoculated that night have germinated a week ago. I mixed up a batch of super soft agar (about 8.5 grams of agar, 10 grams of Light Malt, and 500ml of water) and have squirted another batch of spore solution on there and have been waiting about a week. Nothing. Do Bisporus take a long while to germinate? Do they require something special to encourage them to germinate? I was hoping to have some MS mycelium to work with by now. I mixed up one more batch of agar (soft, 10 gm light malt, 1 gram nutritional yeast, and 3m grams activated charcoal. I'm hoping that might be the magic bullet. I'm excited to try these beautiful pans... but I'm impatient and also a bit anxious about the quality of these spores.
No, I germinated them on water agar 7gr-500ml. Took a week.

Could provide you with a print in time, buy it will take a while before I got some new bisporus
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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 1
#28283122 - 04/18/23 04:10 AM (9 months, 7 days ago) |
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Quote:
PsiloPsychIn said: Hey Folks I bought a Pan Bisporus (Coville WA) spore syringe from a reputable vendor and the date on it is 10/22. There is plenty of dark spore looking stuff in the syringe. To my surprise the first attempt at germinating (2.5 weeks now) there has not been a wiff of germination of any thing. Other spores from prints that I inoculated that night have germinated a week ago. I mixed up a batch of super soft agar (about 8.5 grams of agar, 10 grams of Light Malt, and 500ml of water) and have squirted another batch of spore solution on there and have been waiting about a week. Nothing. Do Bisporus take a long while to germinate? Do they require something special to encourage them to germinate? I was hoping to have some MS mycelium to work with by now. I mixed up one more batch of agar (soft, 10 gm light malt, 1 gram nutritional yeast, and 3m grams activated charcoal. I'm hoping that might be the magic bullet. I'm excited to try these beautiful pans... but I'm impatient and also a bit anxious about the quality of these spores.
The spore quality is definitely fucked. They should germ in a few days like any other spores.
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ruawakeyet

Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 2
#28283143 - 04/18/23 04:37 AM (9 months, 7 days ago) |
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Quote:
PsiloPsychIn said: Hey Folks I bought a Pan Bisporus (Coville WA) spore syringe from a reputable vendor and the date on it is 10/22. There is plenty of dark spore looking stuff in the syringe. To my surprise the first attempt at germinating (2.5 weeks now) there has not been a wiff of germination of any thing. Other spores from prints that I inoculated that night have germinated a week ago. I mixed up a batch of super soft agar (about 8.5 grams of agar, 10 grams of Light Malt, and 500ml of water) and have squirted another batch of spore solution on there and have been waiting about a week. Nothing. Do Bisporus take a long while to germinate? Do they require something special to encourage them to germinate? I was hoping to have some MS mycelium to work with by now. I mixed up one more batch of agar (soft, 10 gm light malt, 1 gram nutritional yeast, and 3m grams activated charcoal. I'm hoping that might be the magic bullet. I'm excited to try these beautiful pans... but I'm impatient and also a bit anxious about the quality of these spores.
You might try some BRF pucks. I had luck one time getting some Mexicana spores to germinate on BRF pucks that refused to germinate on soft agar.
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AspectOfTheCreator
Mastering the Art of Success


Registered: 12/07/22
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
#28283284 - 04/18/23 07:14 AM (9 months, 7 days ago) |
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Quote:
PsiloPsychIn said: Hey Folks I bought a Pan Bisporus (Coville WA) spore syringe from a reputable vendor and the date on it is 10/22. There is plenty of dark spore looking stuff in the syringe. To my surprise the first attempt at germinating (2.5 weeks now) there has not been a wiff of germination of any thing. Other spores from prints that I inoculated that night have germinated a week ago. I mixed up a batch of super soft agar (about 8.5 grams of agar, 10 grams of Light Malt, and 500ml of water) and have squirted another batch of spore solution on there and have been waiting about a week. Nothing. Do Bisporus take a long while to germinate? Do they require something special to encourage them to germinate? I was hoping to have some MS mycelium to work with by now. I mixed up one more batch of agar (soft, 10 gm light malt, 1 gram nutritional yeast, and 3m grams activated charcoal. I'm hoping that might be the magic bullet. I'm excited to try these beautiful pans... but I'm impatient and also a bit anxious about the quality of these spores.
Depending on your agar that might not be soft enough. I use telephone brand and never managed to get reliable germination until I went down to 6.5 g agar per 500 mL H2O. As someone else said, try a BRF puck. As soon as you get germination transfer some myc to agar. Grows out very nicely like that.


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YoshiTrainer
Onion tied to belt



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I agree w/Adas, my agar is pretty firm and my bisporus spores sprouted nicely.
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Rad Aghast



Registered: 12/07/18
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#28283521 - 04/18/23 10:14 AM (9 months, 7 days ago) |
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I'm a noob with Pans. But I have had some success getting stubborn spores to germinate by starting in LC, then going back to agar to clean up.
That being said, I wish I would have just thrown in the towel right away because it wasn't worth getting stubborn spores to germinate when then I spent weeks trying to get them to fruit and they wouldn't do that either.
These days if they don't come to play I don't waste my time with them.
RA
Edited by Rad Aghast (04/18/23 10:14 AM)
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PsiloPsychIn
PsiloPsychIn



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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast] 3
#28283741 - 04/18/23 12:59 PM (9 months, 7 days ago) |
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Hey Pan People!   
Thank you all for the responses and also those who PM'ed with an offer of a fresh print. I was not fishing for that, I thought maybe there was some trick to it like adjusting the pH or something like that. Good to know it wasn't anything I was doing. One small point that came up in the responses that is an interesting one that I've thought of before but is not discussed much here (even in the AGAR thread to my recollection) ... that is agar strength. All recipes assume some sort of standard agar concentration or bloom strength or what ever it is called. However, we all know that not all agar is created alike. Some will make very firm agar with 10gm per 500ml and another will make fairly soft agar with 10gm per 500ml. I wonder if there is some sort food industry metric on the label that I've been missing that would characterize an agar in terms of how much "Gell Power" it has.
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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YoshiTrainer
Onion tied to belt



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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 1
#28283863 - 04/18/23 02:15 PM (9 months, 7 days ago) |
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Like this?
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Meaux Da Fun Guy
Clone Wars



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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#28283896 - 04/18/23 02:50 PM (9 months, 7 days ago) |
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Hello all. My first two attempts bombed but not failed. My current attempt is giving me some mixed results but far from me just chucking it into the compost pile. So here’s my run down and some shroom porn. From print to agar with one transfer before spawning to whole oats. Then a substrate of straw,coco, vermiculite was mixed and pcd for 80 minutes+- Finally 2pint jar of oat spawn was mixed with the substrate into a seedling tray and covered with plastic wrap with a couple holes punched in it for fae. Before covering I did somewhat precased with cvg to hold in moisture.
I seem to be having the exact opposite problem as everybody else being as though mines are growing short and thick and others seem to be growing tall and skinny. And tips or help in anyway would be appreciated.  url=https://files.shroomery.org/files/23-16/183032947-IMG_4148.jpg] [/url]
 
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YoshiTrainer
Onion tied to belt



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Looks like it going to be a nice flush! I think they might be cubes though?
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Meaux Da Fun Guy
Clone Wars



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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#28283961 - 04/18/23 03:42 PM (9 months, 7 days ago) |
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It wouldn’t surprise me. This would be the second time I’ve order pan cyan and got a cube. Smh thanks for the reply.
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PsiloPsychIn
PsiloPsychIn



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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#28284079 - 04/18/23 05:16 PM (9 months, 7 days ago) |
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Quote:
YoshiTrainer said: Like this?

Cool! Yes I have never seen anything like that. I fact before posting that this morning I checked the label of the agar I’m currently using and didn’t see anything like it. How to interpret that ??? Does that mean it takes 700mg of agar to “gellify” 2 cubic centimeters? 🤪
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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Smoothcat
Renegade-master



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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
#28284120 - 04/18/23 05:40 PM (9 months, 7 days ago) |
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Getting the stubborn germination too - been away awhile, as we do, trying to revive some old prints and plates from long term storage. Been nearly three weeks with no action from the spores.
Agar was soft at 8g per 500ml but not super soft so maybe i'll go softer and try again
-------------------- Back once again with the ill behaviour Links I like
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pesa



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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
#28284156 - 04/18/23 06:03 PM (9 months, 7 days ago) |
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Quote:
PsiloPsychIn said:
Quote:
YoshiTrainer said: Like this?

Cool! Yes I have never seen anything like that. I fact before posting that this morning I checked the label of the agar I’m currently using and didn’t see anything like it. How to interpret that ??? Does that mean it takes 700mg of agar to “gellify” 2 cubic centimeters? 🤪
agar with a gel strength of 700mg per 2cm, means that a 2cm x 2cm square of agar gel can hold up to 700mg of weight without collapsing or breaking apart.
if thats Chinese agar, there is strain of E. coli (Escherichia coli) that does not die at 13 psi need to nuke it for longer.
folks in USA are lucky to have "telephone brand agar", the rest is experimenting to find one that can work.
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YoshiTrainer
Onion tied to belt



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Re: Official Copelandia/Panaeolus Thread [Re: Smoothcat]
#28284159 - 04/18/23 06:03 PM (9 months, 7 days ago) |
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Not sure exactly how to interpret it either. Maybe a 2cm piece of agar could support 700mg?
Here is the rest of the label with mixing instructions.

Sweet, just looked this up
"A load is applied on the surface of the thus obtained gel, and a maximum weight of the load, which the gel can withstand for 20 seconds, is measured. The value of the maximum weight per 1 cm 2 is called the gel strength of agar."
On the pan front, I have two shoeboxes that I'm having a staring contest with. I think they're winning.
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Auron
Apologetic noob



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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#28284175 - 04/18/23 06:10 PM (9 months, 7 days ago) |
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I was drying out some manure on a window screen today and noticed a very fine coir-like filtering through. Is that the good stuff or trash?
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pesa



Registered: 06/13/20
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#28284178 - 04/18/23 06:12 PM (9 months, 7 days ago) |
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Quote:
YoshiTrainer said: Not sure exactly how to interpret it either. Maybe a 2cm piece of agar could support 700mg?
Here is the rest of the label with mixing instructions.

Sweet, just looked this up
"A load is applied on the surface of the thus obtained gel, and a maximum weight of the load, which the gel can withstand for 20 seconds, is measured. The value of the maximum weight per 1 cm 2 is called the gel strength of agar."
On the pan front, I have two shoeboxes that I'm having a staring contest with. I think they're winning.
this will work fine. the petri-dish got a size all agar gel strength is made according to amount of liquid a petri-dish will hold in the end its entire diameter act as surface to hold, bacteria, mycelium etc no need to get in to this these things.
for 500 ml you will need to use 7.5g
Edited by pesa (04/18/23 06:12 PM)
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Gorguss
Chaotic sums


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Re: Official Copelandia/Panaeolus Thread [Re: pesa]
#28284358 - 04/18/23 07:30 PM (9 months, 7 days ago) |
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Spawned some questionable pans today, aus and san dose. 2 spots, maybe 3 hard some wet uncolonized grains. Is it unheard of to get pans fruiting from bacterial spawn, assuming it wasn't too prolifically contam'd?
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kirkeng
Stranger


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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss]
#28284443 - 04/18/23 08:29 PM (9 months, 7 days ago) |
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In my experience they never make it when a bit bacterial, but that’s just my experience. Still worth a shot though if you got the time and space.
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Rush56
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
#28284680 - 04/18/23 11:19 PM (9 months, 6 days ago) |
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Just a quick question, since im getting into growing pans. I'm checking various teks and started with what i had at hand - BRF/manure cakes. They are few days from fully colonizing and looking nice. Then i plan to go bulk with these.
Older teks mostly use manure/verm as bulk, on this thread/tek i see people mostly use coir instead of verm. What is the difference between using verm or coir? I wanted to follow one of these older teks with manure/verm, but it drags me more into manure/coir direction now. I have both coir and verm at hand. I haven't noticed this thread before as i was planning to follow some older teks.
I did few brf to bulk with cubes and i prefer using coir, seems less messy. Should i go with coir and only use verm if bulk is wet above field capacity?
Edited by Rush56 (04/18/23 11:23 PM)
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