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myc_ousin_vinny
Keeping_It_Real



Registered: 04/29/20
Posts: 1,415
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Quote:
AspectOfTheCreator said: I used producers pride oats. Going A2G, the pan myc downright refused to jump off onto the oats every single time.
I had nothing but trouble with that brand of oats. Threw my bag out. Not sure why and I know it's anecdotal but... I switched to rye so I'll never know.
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,209
Loc: Castles made of sand
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I use Producers Pride Oats w/no problem when I low-prep.
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myc_ousin_vinny
Keeping_It_Real



Registered: 04/29/20
Posts: 1,415
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#28278637 - 04/15/23 09:06 AM (9 months, 10 days ago) |
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Quote:
YoshiTrainer said: I use Producers Pride Oats w/no problem when I low-prep.
Yeah, it definitely could've been something with my inoculation technique... I had mold in every jar. Was using Bod's prep tek.
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AspectOfTheCreator
Mastering the Art of Success


Registered: 12/07/22
Posts: 1,269
Last seen: 32 minutes, 9 seconds
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I rinsed 5x and boiled for ~35 minutes with tiny amount of gypsum. Watched very closely and removed from heat at the sight of the first burst grain. Strained 30 minutes, spread out, dried for 2 hrs. PCed for 2 hours at 15 psi. Grains were plump and dry on the outside. Freely flowing when the jar was tilted. No clumping or condensation. Regardless, no pan or cube myc would jump off from agar wedge onto the grains.
Even with clearly terribly prepared grains in the past and dirty agar, I would get SOME colonization. Like Vinny said, I never figured it out. Said the hell with it and moved onto different grains.
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psilocybist
All is One



Registered: 05/22/14
Posts: 528
Loc: Far
Last seen: 1 day, 14 hours
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Quote:
AspectOfTheCreator said: I rinsed 5x and boiled for ~35 minutes with tiny amount of gypsum. Watched very closely and removed from heat at the sight of the first burst grain. Strained 30 minutes, spread out, dried for 2 hrs. PCed for 2 hours at 15 psi. Grains were plump and dry on the outside. Freely flowing when the jar was tilted. No clumping or condensation. Regardless, no pan or cube myc would jump off from agar wedge onto the grains.
Even with clearly terribly prepared grains in the past and dirty agar, I would get SOME colonization. Like Vinny said, I never figured it out. Said the hell with it and moved onto different grains.
If this is exactly what you've done, and if this problem occurs every time with this brand, maybe it's because they treat the grains with something ( antifungal and/or bug repellants) they add small amounts to rice around here.
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Rad Aghast



Registered: 12/07/18
Posts: 133
Loc: Minnesota
Last seen: 4 months, 30 days
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist] 11
#28278739 - 04/15/23 10:31 AM (9 months, 10 days ago) |
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Nice canopy of BVI. Kinda small caps but that is what the print looked like so not surprised.
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kirkeng
Stranger


Registered: 02/14/21
Posts: 807
Loc: Lost
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast] 1
#28278747 - 04/15/23 10:37 AM (9 months, 10 days ago) |
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Well done man, those look beautiful!
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AspectOfTheCreator
Mastering the Art of Success


Registered: 12/07/22
Posts: 1,269
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
#28278962 - 04/15/23 01:02 PM (9 months, 10 days ago) |
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Streak from a spore print of BVI onto 0.75% LMEA. Germination or bacteria? Or still too early to tell? Let me know if more pics may help.
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psilocybist
All is One



Registered: 05/22/14
Posts: 528
Loc: Far
Last seen: 1 day, 14 hours
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Take another pic, preferably from side angle. Make the agar surface be visible.
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AspectOfTheCreator
Mastering the Art of Success


Registered: 12/07/22
Posts: 1,269
Last seen: 32 minutes, 9 seconds
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
#28279020 - 04/15/23 01:45 PM (9 months, 10 days ago) |
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Hard to get pics. Camera just wants to focus on the outside of the container.




Pretty sure it's myc. Just want confirmation.
Edited by AspectOfTheCreator (04/15/23 02:02 PM)
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psilocybist
All is One



Registered: 05/22/14
Posts: 528
Loc: Far
Last seen: 1 day, 14 hours
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Quote:
AspectOfTheCreator said: Pretty sure it's myc.
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CocaineBuffet
Stranger



Registered: 08/29/19
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist] 5
#28279638 - 04/15/23 09:25 PM (9 months, 9 days ago) |
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I got some if my first pan pins!!!! Thanks Baba!
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kirkeng
Stranger


Registered: 02/14/21
Posts: 807
Loc: Lost
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Re: Official Copelandia/Panaeolus Thread [Re: CocaineBuffet]
#28279662 - 04/15/23 09:41 PM (9 months, 9 days ago) |
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Can’t think of a better feeling! Best of luck as they mature, and cheers to your inevitable taste tasting.
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: CocaineBuffet] 1
#28279678 - 04/15/23 09:49 PM (9 months, 9 days ago) |
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Ratatoskr
Squirrel


Registered: 07/12/13
Posts: 230
Loc: Roots of Yggdrasill
Last seen: 7 days, 12 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 9
#28281695 - 04/17/23 07:50 AM (9 months, 8 days ago) |
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some BVI trays

-------------------- All is one, one is all, everything is.
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: Ratatoskr] 4
#28281808 - 04/17/23 09:23 AM (9 months, 8 days ago) |
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Nice grow Ratatoskr
Been away a bit and missed the discussion around pasteurization. I'll chime in with an observation I made recently as I was conducting the trich experiments. With coir, and cubes, 130F for two hours killed all trich spores. So on my next batch of pan substrate (straw, manure, verm), I pasteurized it for 6 hours at 130F. The resulting trays contaminated very quickly, and very completely, but not with trich...... it was some other kind of mold. So likely that temp did work on trich, but there are other molds that require higher temps to kill. I will go back to 2 hours at 160-170F which always worked really well for me. The substrate gets a lot more duration than two hours.... I use my steam pasteurizer and have it set to 170F which means that the subs take a bit to get up to the target 160F. Then of course, there is the time it takes them to cool down.
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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 2
#28281852 - 04/17/23 09:59 AM (9 months, 8 days ago) |
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Gray mold is a bitch when it comes to poo-based subs.
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CocaineBuffet
Stranger



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Re: Official Copelandia/Panaeolus Thread [Re: CocaineBuffet] 3
#28282774 - 04/17/23 09:15 PM (9 months, 7 days ago) |
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Quote:
CocaineBuffet said: I got some if my first pan pins!!!! Thanks Baba!

They were actually Semps
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,907
Loc: Rent free in your head
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Re: Official Copelandia/Panaeolus Thread [Re: CocaineBuffet]
#28282831 - 04/17/23 09:43 PM (9 months, 7 days ago) |
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--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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PsiloPsychIn
PsiloPsychIn



Registered: 06/17/14
Posts: 8,182
Loc: up north
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Re: Official Copelandia/Panaeolus Thread [Re: SirPsycho]
#28283048 - 04/18/23 01:43 AM (9 months, 7 days ago) |
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Hey Folks I bought a Pan Bisporus (Coville WA) spore syringe from a reputable vendor and the date on it is 10/22. There is plenty of dark spore looking stuff in the syringe. To my surprise the first attempt at germinating (2.5 weeks now) there has not been a wiff of germination of any thing. Other spores from prints that I inoculated that night have germinated a week ago. I mixed up a batch of super soft agar (about 8.5 grams of agar, 10 grams of Light Malt, and 500ml of water) and have squirted another batch of spore solution on there and have been waiting about a week. Nothing. Do Bisporus take a long while to germinate? Do they require something special to encourage them to germinate? I was hoping to have some MS mycelium to work with by now. I mixed up one more batch of agar (soft, 10 gm light malt, 1 gram nutritional yeast, and 3m grams activated charcoal. I'm hoping that might be the magic bullet. I'm excited to try these beautiful pans... but I'm impatient and also a bit anxious about the quality of these spores.
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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