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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss] * 2
    #28272267 - 04/11/23 04:52 AM (9 months, 14 days ago)

Quote:

Gorguss said:
Where can one find chloramphenicol eye drops? Tried a simple search couldn't find really determine if it contained the stuff or not.




Don't know for where you live, but here it's available in %0.5 concentration, as eye drop, and doesn't require prescription.
(Though this could be true only in my damn country where you can almost get any drug from pharmacy stores without prescription)

Another prefered abtibiotic (which also withstands PC/autoclave) is Gentamicin.
Though doesn't cover as many bacterial species as chloramphenicol does.
This is available in injectable vials (20 and 40 and 80 mg) if you can find them.
You can dilute it in a 50ml syringe to adjust the concentrations you want and then store it cold temps for later use. Cuz no one needs 40 mg gentamicin at once. Unless you're pouring a few thousand plates in one go.
[ALWAYS LABEL THE SYRINGES/VIALS, side effects of accidental exposure to many of these antibiotics are way nasty]

I've tried many antibiotic combinations for agar and LC (penicillin, cefazolin, zoxime, vancomycin, ampicillin, kanamycin, azithromycin, amoxicillin etc...) and the best (as also suggested by some other articles) is a mixture of Chloramphenicol+Gentamicin (16~50 and 4~10 mg/L respectively).
Many of these antibiotics don't cover enough bacteria, many don't stay effective after some time, and many don't withstand sterilization heat.

The recommended combo is great at all aspects. (I've tested open air plates in front of window where airborne stuff can easily sit on media, and nothing but mold could ever start to live on the plate. And this is only at 16 Chloramph. 4 Gent. concentrations. So higher antibiotic content doesn't benefit you/mycelium but actually slows it down as I tested in LC.)

Very high concentrations (around 10 times above) stalls the mycelium. Though after some days in some antibiotic solutions, mycelium started to grow slowly (probably due to inactivation of antibiotic)

Though it's very tempting, DON'T ever add antibiotics to your spawn/substrate/casings. You'll only harvest trichoderma/penicillium/cobweb/aspergillus and other molds.
Many bacteria are beneficiary to your substrate and mycelium does way better in their presence.

Remember antibiotics don't stop molds as molds are fungi as well.

edit : I think it's good to collect/write all my experiments with antibiotics in my journal for others to read. Should do it later.


Edited by psilocybist (04/11/23 05:05 AM)


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OfflineGorguss
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
    #28272458 - 04/11/23 08:35 AM (9 months, 14 days ago)

Yea I've been using water agar now for a few months. Still new to me. I've never used antibiotics and was curious for a source.

You got a brand you use?


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OnlineAspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss] * 1
    #28272464 - 04/11/23 08:41 AM (9 months, 14 days ago)

Pan Cambo myc on water agar. Not as organized as I would have liked. This culture has been troublesome and I don't have anymore spores so trying to make this work.





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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss]
    #28272484 - 04/11/23 09:02 AM (9 months, 14 days ago)

Quote:

Gorguss said:
Yea I've been using water agar now for a few months. Still new to me. I've never used antibiotics and was curious for a source.

You got a brand you use?




We don't care about brands here. :facepalm:
That's how we live. Just as no one cares about any standard. Neither people do, nor factories.
That's probably the only good thing about living in a third world country where nothing is regulated as should be.
I went to drug store, asked for chloramphenicol vial, they said don't have any, I said then an eye drop of that, they gave me.
The brand I have bought last time is called  "chlobiotic" made probably under license of india or russia. Don't know.
But this info is not useful for you. You might ask someone to get it for you :shrug:

I don't know how strict rules are where you live. I'm not gonna suggest you ways to get a drug where it's only sold by prescription. Though antibiotics are not actually "illicit" kinda drugs. (As opposed to sedatives and such)


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: AspectOfTheCreator]
    #28272514 - 04/11/23 09:20 AM (9 months, 14 days ago)

Psilocybist thank for some deeper insight on antibiotic agar. I use a finish agar powder blend with gentamicin. Can you tell me how long lifetime gentamicin have.?


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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #28272571 - 04/11/23 09:54 AM (9 months, 14 days ago)

If by lifetime you mean how long it stays effective as an antibiotic itself, then refer to the vial/bottle it came in.

Expiry date written on product is usually too conservative for this class of antibiotics. You can use gentamicin as agar additives even after 2 years post expiration date. (It's just a recommendation for agar media. Don't ever use expired drugs)

After the vial is broken, (or powder is reconstituted using a liquid, if it'sin powder form) the lifetime reduces considerably.
Medical notes talk about around 2 to 8 hours in room temp, and 24 hours to a week depending on the antibiotic, if kept at 4°C. (Reconstituted antibiotics should NOT get frozen).
This timings are too conservative as well.
And are based on the assumption that the drug is gonna be administered by human/vet.

And if by lifetime you mean inside agar, it strongly depends on the type of antibiotic (it's molecular class)
Gentamicin and chloramphenicol stay effective for at least until agar plate dries out. So no worry there.
In LC, both stay active at least a week (this is a very safe and sure time. Cuz I have tested dirty nutrient liquid media on non-antibiotic agar, 3 weeks after their initial sterilization, and none made bacterial colonies on new plate.)

These observations makes one see antibiotics as a miraculous remedy for all contamination problems. They seem to replace heat sterilization completely, But it's not what it seems.
I'll soon write on my journal, a serie of must-know facts, and also my findings about antibiotics and their uses in this hobby.

Bottom line: antibiotics are useful, but don't rely merely on them. (Specifically bacteriostatic antibiotics,  which don't kill, but inactivate bacteria. Chloramphenicol is one of them)


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
    #28272883 - 04/11/23 01:28 PM (9 months, 14 days ago)

I was intresting to know how long time you think ican store my bought agar powder mix (agar malt peptone yeast and gentamicin)  it don't have a expiry Date on the bottle it came in. I keep it at 4c but I Wonder how long time i can expect the gentamicin to do it's work. I have try to Google a little but didn't find anything usefull.


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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #28272911 - 04/11/23 01:53 PM (9 months, 14 days ago)

You bought pre-mixed antibiotic agar powder?
They always list all ingredients/additives in weight percentages.
Some state the media pH as well.
If it was not labeled on the bottle/box, you may ask the seller
(probably they've opened bigger container and sold in smaller ones)

The only time I bought pre-made antibiotic agar, it was agar+dextrose+chloramphenicol.
iirc, it was %0.3 antibiotic. Gentamicin has a long life, I think you're safe to assume
it's active as long as the agar itself is good. It degrades with UV light (as in sunlight)
and long-time exposure to high temperatures.

If you store it for more than a year, you can easily test it on a single non-sterilized plate.
Pour one, expose it open air, or streak it with bacteria, incubate to see if it shows dense bacterial colonies in days. (Satellite colonies)
If so, the antibiotic is ineffective.


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
    #28273015 - 04/11/23 03:03 PM (9 months, 14 days ago)

Yes it's pre-mixed. I am from scandinavia and here we can not just buy antibiotics. But I have find a place in EU that sell pre-mix

I know it's very expensive for some agar but so is a good flowhood.

The recipt for one liter media=47 gram pre-mix
Ingredients:
24 g agar-agar
20 g malt extract
2 g dry yeast
1 g peptone
Aminoglycoside antibiotics

Now i see the recipt don't says it's gentamicin so maybe thats my mind self made up. But gentamicin is a aminoglycoside antibiotic.
So i will keep working in the unknown.
Are all in the group of aminoglycoside antibiotics have same lifetime.


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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #28273052 - 04/11/23 03:15 PM (9 months, 14 days ago)

Yes nearly all Aminoglycosides withstand heat-sterilization in PC/autoclave.
It most probably contains gentamicin plus kanamycin.
If it's strictly formulated for fungal media preparation, it won't contain Streptomycin.

All aminoglycoside have long lifes, and are ok to be combined with some other antibiotics if you choose to.
(Like Ampicillin for example)


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
    #28273065 - 04/11/23 03:20 PM (9 months, 14 days ago)

Thank you so much expert:thumbup:


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OfflineAuron
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #28273098 - 04/11/23 03:40 PM (9 months, 14 days ago)

Can someone advise where to take from?  P.cyan second transfer from syringe



Edited by Auron (04/11/23 04:31 PM)


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 3
    #28273243 - 04/11/23 05:25 PM (9 months, 14 days ago)

Quote:

Adas said:
Great work everyone!

Psilo, what kinda fool is your friend to take 4g of Pans? :rofl: I've heard of people basically almost blacking out from 2g. Does he have a super high natural tolerance?

On a side note, does anybody know what happened to JCM? Havent seen him in a very long time.




He has a very high tolerance - pretty much a life time of SSRI use.  He generally goes off them for a day or two. This time he’d been off them for 5-6 days.  I warned him. He had asked to do 5. The other guy who did 4 also seems to have a high tolerance.  I gave them APE once and warned them about potency. They had never had them. They both took 10 grams!

The first guy said he was blasted into outer space - had no idea who he was or where he was for hours.  The second guy was also way way out there but when he regained a bit of his identity it was hellish for about 2 hours because he was convinced he’d eaten poisonous mushrooms and permanently wrecked his brain - so he was convinced he’d lose his job, his house, his wife and kids! He was in agony about the biggest mistake he’d ever made in life. When he finally came down enough to realize his condition was not permanent, he felt he’d been given his life back and the rest of his trip was ecstatic.

Like you, I’ve been very worried about JCM.  Before he disappeared his brother (who he was very close to) had committed suicide. He had a very heavy morning period. Their parents were so devastated the responsibility of dealing with his brothers estate fell on him… (not a rich guy but had stuff that needed to be dealt with).

It was a very rough time for him but after a long silence- I did see him come back in here a bit then and then he disappeared again.

I’ve sent him PMs on here but no response!
I had a personal email  for him. Maybe I’ll try that again.

Cool Dude - hope he is ok.


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OfflineAuron
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #28273314 - 04/11/23 05:53 PM (9 months, 14 days ago)

Quote:

PsiloPsychIn said:
Quote:

Adas said:
Great work everyone!

Psilo, what kinda fool is your friend to take 4g of Pans? :rofl: I've heard of people basically almost blacking out from 2g. Does he have a super high natural tolerance?

On a side note, does anybody know what happened to JCM? Havent seen him in a very long time.




He has a very high tolerance - pretty much a life time of SSRI use.  He generally goes off them for a day or two. This time he’d been off them for 5-6 days.  I warned him. He had asked to do 5. The other guy who did 4 also seems to have a high tolerance.  I gave them APE once and warned them about potency. They had never had them. They both took 10 grams!

The first guy said he was blasted into outer space - had no idea who he was or where he was for hours.  The second guy was also way way out there but when he regained a bit of his identity it was hellish for about 2 hours because he was convinced he’d eaten poisonous mushrooms and permanently wrecked his brain - so he was convinced he’d lose his job, his house, his wife and kids! He was in agony about the biggest mistake he’d ever made in life. When he finally came down enough to realize his condition was not permanent, he felt he’d been given his life back and the rest of his trip was ecstatic.

Like you, I’ve been very worried about JCM.  Before he disappeared his brother (who he was very close to) had committed suicide. He had a very heavy morning period. Their parents were so devastated the responsibility of dealing with his brothers estate fell on him… (not a rich guy but had stuff that needed to be dealt with).

It was a very rough time for him but after a long silence- I did see him come back in here a bit then and then he disappeared again.

I’ve sent him PMs on here but no response!
I had a personal email  for him. Maybe I’ll try that again.

Cool Dude - hope he is ok.




Holy hell, so 1g sounds like more than enough?  Does this make them more inconsistent than cubes?


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InvisibleMr Piggy
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Re: Official Copelandia/Panaeolus Thread [Re: Auron]
    #28273456 - 04/11/23 07:26 PM (9 months, 13 days ago)

I cannot wait to eat pans.  4 grams of psilocybe cyanescens barely even makes me flinch.  I've had an unnaturally high tolerance for a while and it's the pits when you're trying to get there.


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Re: Official Copelandia/Panaeolus Thread [Re: Mr Piggy] * 7
    #28273607 - 04/11/23 09:22 PM (9 months, 13 days ago)

Couldn’t get the bisporus to fruit indoors in a shoebox but buried the sub a short ways into the forest and they are popping nicely.



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OfflineFred_feuerstein69
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Re: Official Copelandia/Panaeolus Thread [Re: Mr Piggy]
    #28273611 - 04/11/23 09:24 PM (9 months, 13 days ago)

Quote:

Mr Piggy said:
I cannot wait to eat pans.  4 grams of psilocybe cyanescens barely even makes me flinch.  I've had an unnaturally high tolerance for a while and it's the pits when you're trying to get there.




If 4gr cubes have almost no impact, it means your serotonin receptors are worn out..most likely long term pharmaca or other drug use.

I wonder what people wanna get out of it anyway? Majority just abuses the drug anyway.. All the people I know took shrooms onces and would never try again.

Wrong setting, wrong intensions. Than shrooms will not help cos they simply know.

They have only one purpose: show the matrix you in and understand that you been creating it. Understanding inner issue that make you take shrooms in first place.

Everything else is just abuse for fun and temporarily escaping reality.

If someone doesnt do self enquiry and self observation into this reality and get the fat EGO in control shroom use doesnt bring anything and just confuse them even more.

In the end every shroom trip is your own self reflection.


Edited by Fred_feuerstein69 (04/11/23 11:36 PM)


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OfflineGorguss
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
    #28273867 - 04/12/23 04:04 AM (9 months, 13 days ago)

Quote:

psilocybist said:
Quote:

Gorguss said:
Yea I've been using water agar now for a few months. Still new to me. I've never used antibiotics and was curious for a source.

You got a brand you use?




We don't care about brands here. :facepalm:
That's how we live. Just as no one cares about any standard. Neither people do, nor factories.





Yea same, I was only interested in nailing down what to look for. For me once I can read the ingredient label of something known to be right it's easier to reference that to find it. Thank you for your help!

I took advice and have steered clear of antibiotics for years now. I get the logic and reasoning, but I'm still interested in working with it.


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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss]
    #28273872 - 04/12/23 04:11 AM (9 months, 13 days ago)

I'm close to finishing writing a post on somewhat general info and must-know things about antibiotics.
Will put a link here and in my signature soon. Hope it helps many so no one makes my mistakes again.
And it hopefully will encourage others to experiment with new methods.


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Southerner]
    #28273890 - 04/12/23 04:26 AM (9 months, 13 days ago)

Quote:

Southerner said:
Couldn’t get the bisporus to fruit indoors in a shoebox but buried the sub a short ways into the forest and they are popping nicely.






Hell yeah buddy! This is like music to my eyes, I love to see Bisporus being done outdoors! Can't wait to spread mine! Did you also mix any other sub with it or did you just bury the colonized stuff you had?


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