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OfflineRad Aghast
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Registered: 12/07/18
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28265346 - 04/06/23 01:56 PM (9 months, 19 days ago)

I have to pasteurize some sub this weekend. I think I'll do a longer process at 140 (60C) and age for 3 days. No side to side here but just for kicks.

The thing they mentioned about smell has caught my attention before. A freshly pasteurized sub will smell up a whole room through a ziploc bag. But after a few days it stops wreaking and actually smells kinda good. :cool:



NICE HARVEST OFOW! 


Edited by Rad Aghast (04/06/23 01:59 PM)


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OfflineMurphy07
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast] * 3
    #28265499 - 04/06/23 03:26 PM (9 months, 19 days ago)

Funny you say that. I literally just tried my first try at pastuerizing seeing as how I just came into contact local field aged hpoo. Being a rookie all I did was take a very large mason jar put some foil over the top and put my wireless Bluetooth meat thermometer through the foil.(you can set target temps) It was a pain in the ass to get it up to temp but I added more water to the pot, which just has boiling water in it and it raised fine and actually stayed at 140 once i turned the temp to med for the whole time. Not sure if coincidence or not but once it got to 140 and I turned it down it stayed steady.

In other news I got my first pan pins today and couldn't be more thankful to you guys and the man who sent the print and his tek. Not out of the woods yet but I'm optimistic af.




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OfflineRad Aghast
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Registered: 12/07/18
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Loc: Minnesota
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Re: Official Copelandia/Panaeolus Thread [Re: Murphy07]
    #28265561 - 04/06/23 03:55 PM (9 months, 19 days ago)

I also have one of those thermometers and do not regret that purchase one bit. Very handy tool for pasteurization. A large sous vide and that thermometer has really allowed me to dial it in.

congrats on the pins!


Edited by Rad Aghast (04/06/23 03:57 PM)


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast] * 9
    #28265984 - 04/06/23 07:21 PM (9 months, 18 days ago)

Second flush of the Komodo - not nearly as prolific, but on average bigger fruits I think.







I never pay attention to such things but since this is a pretty novel variety, I thought folks might be curious.  26.25 dried grams for first flush. 8.75 dried grams from the second flush - so 35 grams so far. It looks to me that it still have some reserves … we’ll see if there is a third flush 🤷♂️


--------------------
What are they saying? Listen carefully, it might be something you need to hear...





Edited by PsiloPsychIn (04/06/23 08:29 PM)


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OfflineFred_feuerstein69
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Registered: 10/04/21
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
    #28266096 - 04/06/23 09:00 PM (9 months, 18 days ago)

Quote:

Rad Aghast said:
That's an interesting read. Thanks for sharing. What would you say are the key take-aways for pan growers?


One thing I might do differently is pasteurize at exactly 140. I do sous vide which I love because I can dial it in +/- 1 deg. I have been leaving water temp at 160 simply because the warmup of the substrate to 140 takes less time. But maybe a slower pasteurization at a temp more beneficial to good microbes is worth the wait.




simply sterilize all and you safe to go. its been done by growers like angam/asura/blue helix. they dont even need grains for this Tek.  LC straight into sterilized sub. gordo also did some test on this subject. no visible difference if sub was past or sterilized.


Edited by Fred_feuerstein69 (04/06/23 09:39 PM)


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OfflineGenome794
Spore-Whore-der & Agar-Addict


Registered: 07/20/22
Posts: 438
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Re: Official Copelandia/Panaeolus Thread [Re: Fred_feuerstein69]
    #28266309 - 04/07/23 04:18 AM (9 months, 18 days ago)

I don't know how Mr Flintstone grows...

I have had good results (on cubes) from Frank's Proper Pasteurisation 3.0... His start temp is also 60/140... Somewhere in the thread I think he says 71/160 and then else where 74/165 and then somewhere else he says 80/175 is the upper boundary and then the sub must not be up there for more than a few minutes.

Getting back to pans...
I am still trying to get to some clean pan cyan from a bad syringe. Started the 3rd round of germ plates yesterday. 1ml syringe, 2ml sterile water,streak 12 plates.


EDIT: Here's the tek
Frank's Proper Pasteurization Tek 3.0


Edited by Genome794 (04/07/23 04:23 AM)


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Offline0t0lerance
I'm a teapot

Registered: 05/01/21
Posts: 962
Loc: The Netherlands Flag
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #28266315 - 04/07/23 04:35 AM (9 months, 18 days ago)

Quote:

PsiloPsychIn said:
Second flush of the Komodo - not nearly as prolific, but on average bigger fruits I think.







I never pay attention to such things but since this is a pretty novel variety, I thought folks might be curious.  26.25 dried grams for first flush. 8.75 dried grams from the second flush - so 35 grams so far. It looks to me that it still have some reserves … we’ll see if there is a third flush 🤷♂️




Look at that, beautifull. What are the fruits like; sturdy, with decent stipes like pan bisporus? Or more like other pans thin/fragile?

I've made some T0, as I used tons of spawn and substrate for my outdoor growth.. but as it condensed due to rain and became a muddy mess... So I'll do an indoor growth. Read these things contain a large amount of norbaeocystin.


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Offlinepsilocybist
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Re: Official Copelandia/Panaeolus Thread [Re: Genome794] * 2
    #28266321 - 04/07/23 04:44 AM (9 months, 18 days ago)

Most (if not all) pasteurization guides care only about substrate core temp.
Proper pasteurization temps (and timings) are well determined through experiments and trials. So it's not that ambiguous/unknown.

Stay ideally @64~75 for 2 to 4 hours.
Cool down fast, and let substrate rest for at least 3 days at somewhat higher temp than room temp.

Dry substrates don't get pasteurized well.
Too wet substrates are more prone to get contaminated after pasteurization than dry substrates.
Under-temp pasteurized substrates are more resistant to contam than over-temp pasteurized. (Both weaker than properly pasteurized substrate)


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InvisibleBaba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist] * 3
    #28266338 - 04/07/23 05:09 AM (9 months, 18 days ago)

The last pasteurization I did was in a presto on a hot plate controlled by an inkbird. A 10 liter bucket fits the PC perfectly. The inkbird was set to 60oC but the actual water bath temperature fluctuated between 55oC and 65oC due to the nature of the setup. Kept it going over night. Worked a charm.


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Offline0t0lerance
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Registered: 05/01/21
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Loc: The Netherlands Flag
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
    #28266340 - 04/07/23 05:15 AM (9 months, 18 days ago)

Forgot to mention. I'm thinking about changing things up and going for a martha set-up.. looks a bit cleaner than the pile of tubs I'm running at the moment.

Already found Jakes thread for a martha setup for pans, this one:
https://www.shroomery.org/forums/showflat.php/Number/26235096/fpart/1/vc/1
but wondered if there is some consensus about this. Would like to run exotics, edibles(oyster) & medicinal mushrooms (reishi) in there too.

Who can point me to the best thread or maybe most bullet proof setup? :rockon: Warm regards from the Netherlands.


..I'm a bit done messing around with things, so I need a set-up which has proven itself over time.


Edited by 0t0lerance (04/07/23 05:17 AM)


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: 0t0lerance]
    #28266439 - 04/07/23 07:08 AM (9 months, 18 days ago)

Fancy, Baba! Love the idea.


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Offlineold_fart_out_west
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #28266726 - 04/07/23 10:46 AM (9 months, 18 days ago)

This jar (T2 TBVI) has been fully colonized for about 2 weeks. Are all of the small white dots knots? And are those some pins I see being pushed up against the glass?

If so, think it’s too late to mix with substrate or should I go for it?



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OfflineYoshiTrainer
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Re: Official Copelandia/Panaeolus Thread [Re: old_fart_out_west] * 3
    #28266825 - 04/07/23 12:01 PM (9 months, 18 days ago)

Don't tell anybody but I sterilize my subs and casings for all my grows, except outdoors. :smile:


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OfflineExcess Taters
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
    #28266856 - 04/07/23 12:24 PM (9 months, 18 days ago)

Low prep the world eh?  They say if you wanna change the world you gotta start by changing yourself.


--------------------
If you've never grown mushrooms before, here's how you start.  First, follow the Updated PF Tek, put 4 holes in jar lids not 1, and use a water tub! 
The next move is Shoebox Tek. After that you move onto grains, agar, monotubs.  Agar is easy, just do it.
Other useful links - Picture guide for how things should look and proper surface conditions guide
Growing APE or PE?  P9 pseudo casing tek


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OfflineEugene Gesuale
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Re: Official Copelandia/Panaeolus Thread [Re: old_fart_out_west] * 2
    #28266913 - 04/07/23 01:03 PM (9 months, 18 days ago)

Quote:

old_fart_out_west said:
This jar (T2 TBVI) has been fully colonized for about 2 weeks. Are all of the small white dots knots? And are those some pins I see being pushed up against the glass?

If so, think it’s too late to mix with substrate or should I go for it?





These look very unhealthy


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.


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OfflineGenome794
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Registered: 07/20/22
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Re: Official Copelandia/Panaeolus Thread [Re: Eugene Gesuale]
    #28266918 - 04/07/23 01:12 PM (9 months, 18 days ago)

Quote:

Eugene Gesuale said:
Quote:

old_fart_out_west said:
This jar (T2 TBVI) has been fully colonized for about 2 weeks. Are all of the small white dots knots? And are those some pins I see being pushed up against the glass?

If so, think it’s too late to mix with substrate or should I go for it?





These look very unhealthy



Yeah...:whathesaid:
Too uneven and too much liquid. It looks like a few grains aren't colonised which AFAIK means theres some bacteria in there too. If they were mine, I would dump 'em, sorry.

But wait for a more experienced Pan grower to weigh in.


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OfflineMurphy07
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Re: Official Copelandia/Panaeolus Thread [Re: Genome794] * 10
    #28267089 - 04/07/23 03:17 PM (9 months, 18 days ago)

Pin city Boys



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OfflineFred_feuerstein69
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
    #28267920 - 04/07/23 10:33 PM (9 months, 17 days ago)

Quote:

YoshiTrainer said:
Don't tell anybody but I sterilize my subs and casings for all my grows, except outdoors. :smile:





dont bust the myth about keeping the healthy bacteria alive.. lol

never seen any difference between  past or a sterile sub..just less work and  lesser chance of the nasties with the sterile option..


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InvisibleMr Piggy
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Re: Official Copelandia/Panaeolus Thread [Re: Fred_feuerstein69] * 4
    #28267935 - 04/07/23 10:47 PM (9 months, 17 days ago)

Got any pics of those grows?


--------------------
🅃🄴🄰🄼 🄵🄾🄸🄻


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OfflineLeftfield420
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #28267962 - 04/07/23 11:35 PM (9 months, 17 days ago)

nice Psilo!

my experimental tubs tanked on me... pretty sure it was because I rushed putting a clone to grain, I'll try it again with spawn that isn't questionable next time...

put some spores to grain tonight... nec-d, wild coast, and pan jam... 🤞🤞 I get some growth I can work with


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