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InvisibleMycolorado
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Re: Official Copelandia/Panaeolus Thread [Re: Leftfield420] * 3
    #28220201 - 03/08/23 07:03 AM (10 months, 17 days ago)

Quote:

Leftfield420 said:
Anyone tried hydrating coir using cow shit compost tea for pans?



Baba: https://www.shroomery.org/forums/showflat.php/Number/28101427


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OfflineLeftfield420
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Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado]
    #28220331 - 03/08/23 08:28 AM (10 months, 17 days ago)

that's where I got the idea Mycolorado... but I'm gonna try just using poo water(compost tea) instead of the way BabaYaga makes that


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InvisibleMycolorado
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Re: Official Copelandia/Panaeolus Thread [Re: Leftfield420] * 1
    #28220378 - 03/08/23 08:56 AM (10 months, 17 days ago)

Pretty sure there was another write up a few years ago doing a similar approach but can’t recall who it was.


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Offlinebrshroomer
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Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado] * 3
    #28220393 - 03/08/23 09:09 AM (10 months, 17 days ago)

Pinset coming in looking good :awesome:

At least something I'd be proud of for a first try



There's one thicc boi in the right corner looking promising



They're mostly growing in clusters...

My substrate depth is also only like an inch, one quart per tray, 1:2 spawn ratio....as I understand most guys are running deeper substrates and that's what I'll try next time...

Figured that water in the sub and FAE look pretty important for these.


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Invisibleabductee
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Re: Official Copelandia/Panaeolus Thread [Re: brshroomer]
    #28220424 - 03/08/23 09:35 AM (10 months, 17 days ago)

Fantastic, I love clusters.


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Invisibleabductee
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Re: Official Copelandia/Panaeolus Thread [Re: abductee]
    #28220434 - 03/08/23 09:38 AM (10 months, 17 days ago)

Just thinking, clusters probably give more meat at the base for cloning thinner species, plus you get clusters in return.. in hindsight I've never fruited a cluster clone culture, I just remember reading that.


Edited by abductee (03/08/23 09:39 AM)


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InvisibleSeeker604
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Re: Official Copelandia/Panaeolus Thread [Re: the_prodigy]
    #28220479 - 03/08/23 10:09 AM (10 months, 17 days ago)

Quote:

the_prodigy said:
The tissue cloning of these pans is basically impossible, stipes are 1mm thick.




It’s totally possible. Follow these steps in front of your flow hood:

Take the cultivar you want to clone. Using a sterile scalpel, cut the stipe perpendicular to the cap at the top of the stipe; sterilize the blade; cut the stipe perpendicular to where it attaches to the substrate; sterilize blade; cut the stipe lengthwise exposing inner tissue. Using sterilized medical tweezers pull off a thin strip of tissue, making sure it is inner tissue only; no external stipe tissue. Plate immediately.

Finally, realize with Panaeolus just like other species, the fuller your first flush canopy, the thinner, more sparse all subsequent flushes will be.

Jake Oncid’s tek here on the site has pictures of healthy clone plates.

Enjoy the fruits of your labor!


πŸ„πŸ’™πŸ„


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Leftfield420] * 2
    #28220520 - 03/08/23 10:31 AM (10 months, 17 days ago)

Quote:

Leftfield420 said:
Anyone tried hydrating coir using cow shit compost tea for pans?




This is how I do mine, basically like tea only a bit thicker cause I pour the solids as well. Ending up with maybe 20% manure in the sub. Pasteurizing the tea and hydrating coir with it is super easy and practically no limit to how much you can prep at once.

Liquid Manure Pasteurization



EDIT: totally skipped the posts after yours Leftfield :facepalm::wink: Looking forward to your trials.


Edited by Baba Yaga (03/08/23 11:42 AM)


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Seeker604] * 4
    #28220535 - 03/08/23 10:40 AM (10 months, 17 days ago)

Quote:

the_prodigy said:
The tissue cloning of these pans is basically impossible, stipes are 1mm thick.





Try cloning to water agar. You can just cut up the stem and place it as is, no inner tissue necessary.



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OfflineRad Aghast
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Re: Official Copelandia/Panaeolus Thread [Re: the_prodigy]
    #28220597 - 03/08/23 11:30 AM (10 months, 17 days ago)

Quote:


Quote:

Rad Aghast said:
What is the empty tote in the top level of your martha for?



Nothing special, I have a tube through top of the tent where the misting go inside and the water drips from the tube into that empty tote.







Cool thx - I have heard of folks putting empty containers in their marthas to try to dial in the cycling. Was just wondering if that was still happening.


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OfflineBlueAndOrange
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
    #28220650 - 03/08/23 12:10 PM (10 months, 17 days ago)

I’ve read through a number of teks related to pans. I really want to get them going this spring. So my question:

What is the easiest path to substrate? I’m going to try both baba yaga’s and hindsight’s grow methods. But when it comes to substrate, what is my best path?

I’m not interested in working with raw horse manure (which I can get). I don’t want to wait to field age it myself. So any thoughts? Is there stuff I can buy and mix that doesn’t involve aging locally sourced poo that will deliver?


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Invisiblecooleko
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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange] * 2
    #28220680 - 03/08/23 12:29 PM (10 months, 17 days ago)

I had great success with Home Depot's manure compost mix with some straw and verm added in. I pasteurize about 8 quarts of the mix (4 manure compost, 2 straw, 2 verm) in my sous vide for 6 hours.


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InvisibleSeeker604
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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange]
    #28220798 - 03/08/23 01:42 PM (10 months, 17 days ago)

Quote:

BlueAndOrange said:
I’m not interested in working with raw horse manure (which I can get). I don’t want to wait to field age it myself. So any thoughts? Is there stuff I can buy and mix that doesn’t involve aging locally sourced poo that will deliver?




According to Jake in his Pans master class, this species is a grass lover more than dung lover. In his experience, the best results came from substrate with a lower manure ratio by volume. His recommended recipe (which I’ll be using for my runs) is by volume:  65% chopped straw (not hay) / 30% manure / 5% gypsum.

Several trusted vendors sell pre-aged, dehydrated manure on their websites (I use Shroom Supply’s). Jake said to make sure if you use store bought manure that it doesn’t have compost in it; should just be field aged, grass fed manure. You don’t use fresh.

I’m waiting until May to get my Pan grows going, but will document the recipes used in my LAGM journal.

Also, Jake sells pre-mixed substrate for Pans specifically if you don’t want to mix it yourself. I can send you the link if you’re interested.

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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange] * 3
    #28221205 - 03/08/23 05:52 PM (10 months, 16 days ago)

Quote:

BlueAndOrange said:
I’m not interested in working with raw horse manure (which I can get). I don’t want to wait to field age it myself. So any thoughts? Is there stuff I can buy and mix that doesn’t involve aging locally sourced poo that will deliver?





Buy the ready made stuff if you don't mind paying top dollar for a bag of straw and manure just pasteurize it yourself and don't get the pre pasteurized stuff. If you want easy and cheap then buy compost that contains manure and pasteurize that, mix with bucket-tek coir and you are done. Member 99.99 posted a successful grow using the homedepot brand steer blend in this thread, he didn't even pasteurize it:

https://www.shroomery.org/forums/showflat.php/Number/28081356#28081356

I personally would pasteurize it though to be on the safe side.


Bottom line is that a lot of things work besides just straw and manure and most of them do work well enough to not having to start a discussion about which one is better. The grass lover / dung lover discussion doesn't really leads anywhere and only limits possibilities for no reason. Your life will be easier without it.


Edited by Baba Yaga (03/08/23 06:12 PM)


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OfflineScotay
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28221505 - 03/08/23 09:23 PM (10 months, 16 days ago)

Prodigy..... Damn that's some sick mush growth!!! I wanna be your neighbor!!!! I'm jelly.
I know I say this often but I really do appreciate how everyone shares their information and teks!! It's great.
Baba..... I'm definitely gonna try the shit water πŸ’¦ combined with coir when I actually get past this grain step. Starting to wonder if my oats are bad. I've gotten the agar to work for me but after I put it on the grain it turns trich. I even tried PC on 15 for 2 hours to try to get it to work but same result. I'm gonna give this one more time and if not I'm going to try another grain. I am going to try another kind (probably P.Nat) on these grains too just to be sure it's not me just fuckin it up!! I'll try like 10 jars and 50/50. 5 pans and 5 pnat. Well see. I put 8 to agar. 4 a piece. Well see what happens!! Hurry up and wait!! Happy Wednesday you filthy panheads!!!
😎✌️


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] * 2
    #28221576 - 03/08/23 10:53 PM (10 months, 16 days ago)

Check your sterile technique, your agar work probably needs to be better or your jar lids are not up to specs.

You gonna start posting few photos soon?


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OfflineSingularFusion
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28221586 - 03/08/23 11:08 PM (10 months, 16 days ago)

yes just to add I have been running entirely strawless so far also fellas

mine is a bagged composted manure at about 30%, pasteurized

then added to standard bucket tek coir 70%

works alright and is easy prep

straw, straw pellets and hemp straw will also be trialed once I have a respectable clone, but for starters this produces decent results for us so far


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Invisibleekabpils
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Re: Official Copelandia/Panaeolus Thread [Re: Seeker604] * 4
    #28221634 - 03/08/23 11:59 PM (10 months, 16 days ago)

Currently I do experimentation with substrate components and structure.
The recent substrate is composed from:

1) Coco coir (https://www.biobizz.com/producto/cocomix/) (7 parts)
2) Dehydrated horse manure from the store (3 parts after hydrated)
3) Verm (2 parts)
4) Organic soil improver Biotabs Startex 60 gr (https://biotabs.nl/en/product/startrex/)

It was pasterized for 12 hours with 66-67C and then left to stay for a few days.

I have cased 4 different cultures (Jalisco, Texas, Bali and Africa) - 1 litre grain for 2.5 litre substrate.

The cakes were taken extremely fast by all strains for 4-5 days completely. And what is interesting - ΠΌycelium did not swirl around the grains, but immediately evenly distributed throughout the tray. Previously, only some isolated cultures of NecD showed such substrate colonization rates.

Yesterday I put it on fruit - we'll see what the harvest will be. Will keep updated.


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Edited by ekabpils (03/09/23 02:45 AM)


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OfflineSingularFusion
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
    #28221645 - 03/09/23 12:19 AM (10 months, 16 days ago)

interesting that you added startrex to that mix

is your current theory there that the added beneficial soil microbes play a role in protecting the pasteurized substrate?

I was gonna try something similar but using EM-1 and woodchips for woodlovers... wonder how mostly lacto strains of bacteria play with indoor things like Pans too now that I have seen you do this

please keep us posted


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: SingularFusion]
    #28221722 - 03/09/23 02:14 AM (10 months, 16 days ago)

You add the startrex before pasteurization? Will the added bacteria survive or is it for the fulvic acid?


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