|
Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 4 days
|
Re: Official Copelandia/Panaeolus Thread [Re: brshroomer] 2
#28210671 - 03/02/23 07:38 AM (10 months, 23 days ago) |
|
|
Quote:
brshroomer said: Just some pics of my first Pan cyan grow ever.
Wild print Spore syringe to wheat (wanted to select from the largest pheno pool)

Spawned to cowpoo/coir 50/50 at 1:2

Planning on casing soon...impressed that these spores were injected day 14...jars are 500ml, but I thought it was pretty fast
Something about that jar looks odd - like there is perhaps some yeast contamination in there. Note the browning/yellowing in the center that goes upward a bit. Other areas of the jar look ok. Maybe it's just the lighting.
RE questions about substrate and sterilization vs pasteurization: I've used man subs but keep coming back to 1:1:1 Manure/Straw/Verm. Coir/Manure is another good option (I would do 1:3 manure/coir or even 1:4 manure coir), but I still prefer straw to coir for pans.
As for sterilization, you CAN, but pasteurization is known to cause less issues with contamination during your fruiting stage because you are preserving beneficial bacteria that help fight off contamination. Sterilization works but most everyone who does it is pairing it with a growing method that allows 100% colonization of that sterile substrate prior to exposing it to the open air. In other words, you grow it like a PF cake - inoculate the sterile cake using sterile procedures and keep it sealed until 100% colonization. This is different from the typical agar to grain spawn to substrate process where you are mixing sterile grain spawn with a substrate in open air not using sterile conditions, and exposing the tub/substrate to open air during colonization phase. That is the risky part. You can do it and it can work but is known to have higher chance of contamination.
|
abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#28210880 - 03/02/23 11:02 AM (10 months, 23 days ago) |
|
|
I took a couple transfers from my pan rainbow plate, it was almost a fully colonized small petri,and also put a couple little wedges to wbs. The sab work was a little shaky while cutting and trying to get it off the blade. I guess I'll see what happens.
|
rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 2 hours
|
Re: Official Copelandia/Panaeolus Thread [Re: abductee]
#28210907 - 03/02/23 11:23 AM (10 months, 23 days ago) |
|
|
Hey pan peeps. Agar keeps showing these grey/blue raised circular spots. Seen it on 4 diff pans now. I figured it was contam at first, but now I'm wondering if I'm pearling.
Is pearls a pan cinct only thing? My pans are in the mid 50s and did freeze at one point. Leading me down the sclerotia path.
 Not the greatest image. They start off as white balls then turn blue/grey.
|
Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 4 days
|
Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
#28210996 - 03/02/23 12:23 PM (10 months, 23 days ago) |
|
|
I've never seen that on a plate Rocky, however, I also don't look at old plates that have been around for over a month because all cultures can do really weird shit after being left in a plate for a really long time. I very commonly see little white spots of concentrated mycelium in pan grain jars, but not on plates. I wouldn't use that plate - personally.
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 8 minutes, 49 seconds
|
Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
#28211018 - 03/02/23 12:42 PM (10 months, 23 days ago) |
|
|
Quote:
rockyfungus said: Hey pan peeps. Agar keeps showing these grey/blue raised circular spots. Seen it on 4 diff pans now. I figured it was contam at first, but now I'm wondering if I'm pearling.
Is pearls a pan cinct only thing? My pans are in the mid 50s and did freeze at one point. Leading me down the sclerotia path.
 Not the greatest image. They start off as white balls then turn blue/grey.
It happens sometimes. To me it tended to happen at high temps in summer. Even Bisporus did it in the heat.
|
TheDoobsker
Amateur


Registered: 05/22/20
Posts: 799
|
Re: Official Copelandia/Panaeolus Thread [Re: Adas] 2
#28211060 - 03/02/23 01:15 PM (10 months, 23 days ago) |
|
|

well god damnit...
|
brshroomer
Moss bear hunter



Registered: 12/10/06
Posts: 970
Last seen: 9 months, 27 days
|
Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
#28211080 - 03/02/23 01:35 PM (10 months, 23 days ago) |
|
|
Quote:
rockyfungus said: Hey pan peeps. Agar keeps showing these grey/blue raised circular spots. Seen it on 4 diff pans now. I figured it was contam at first, but now I'm wondering if I'm pearling.
Is pearls a pan cinct only thing? My pans are in the mid 50s and did freeze at one point. Leading me down the sclerotia path.
 Not the greatest image. They start off as white balls then turn blue/grey.
I'm getting those as well on one culture
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 8 minutes, 49 seconds
|
Re: Official Copelandia/Panaeolus Thread [Re: TheDoobsker]
#28211105 - 03/02/23 01:58 PM (10 months, 23 days ago) |
|
|
Quote:
TheDoobsker said:

well god damnit...
Bruh, your clouds are escaping
|
TheDoobsker
Amateur


Registered: 05/22/20
Posts: 799
|
Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#28211135 - 03/02/23 02:26 PM (10 months, 23 days ago) |
|
|
These aren't mushrooms?
|
Seeker604


Registered: 11/30/20
Posts: 331
Loc: United States
|
Re: Official Copelandia/Panaeolus Thread [Re: uninc4life2010]
#28211136 - 03/02/23 02:29 PM (10 months, 23 days ago) |
|
|
Quote:
uninc4life2010 said: Can someone recommend a good Pan bulk substrate for loading into spawn bags? I plan on using Asura's method, but it's very manure heavy, and manure isn't as easy for me to get ahold of. I'd also like to avoid using vermiculite due to cost reasons. Plus, I don't like having an addition ingredient that I need to keep stock of. I was considering the following recipes for spawn bags:
50% horse manure 25% coir 25% hydrated oats Handful of gypsum
or
33% horse manure 33% coir 33% hydrated oats Handful of gypsum
Would either of these work for sterilized spawn bags?
Pans are a grass loving species, not manure loving. You should dial back the manure and add straw (not hay).
According to JOC, his best results are when he uses grass fed bovine manure that’s field aged, and he gives this ratio by volume: 65% straw 30% manure 5% vermiculite
Hope that helps.
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 8 minutes, 49 seconds
|
Re: Official Copelandia/Panaeolus Thread [Re: Seeker604] 2
#28211138 - 03/02/23 02:31 PM (10 months, 23 days ago) |
|
|
Where did you get the idea that Pans are not dung-loving? Cyans grow straight out of it.
|
uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
|
Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#28211456 - 03/02/23 06:37 PM (10 months, 22 days ago) |
|
|
Quote:
Adas said: Where did you get the idea that Pans are not dung-loving? Cyans grow straight out of it.
I'm wondering about this as well. I though that people just supplemented with straw and coir to fluff up the manure. IME, it can turn to mud really easily. Plus, field-aged manure is just hard to come by for some. I ordered a box of it off ebay, lol.
|
ruawakeyet

Registered: 05/03/21
Posts: 1,862
|
Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#28211526 - 03/02/23 07:09 PM (10 months, 22 days ago) |
|
|
Quote:
Adas said: Where did you get the idea that Pans are not dung-loving? Cyans grow straight out of it.
JakeonCid has said he thinks they are really more grass-loving than dung-loving.
|
uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
|
Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#28211807 - 03/02/23 10:20 PM (10 months, 22 days ago) |
|
|
Does anyone have experience with the Alabama strain of Pan Cyanescens? Is it indiscernible from other Pans? I have a print on order. I guessed that since it's MS, the genetics vary widely until a single strain is isolated.
|
Seeker604


Registered: 11/30/20
Posts: 331
Loc: United States
|
Re: Official Copelandia/Panaeolus Thread [Re: uninc4life2010]
#28211870 - 03/02/23 11:17 PM (10 months, 22 days ago) |
|
|
Quote:
uninc4life2010 said: Does anyone have experience with the Alabama strain of Pan Cyanescens? Is it indiscernible from other Pans? I have a print on order. I guessed that since it's MS, the genetics vary widely until a single strain is isolated.
You could reach out to Jake Oncid. He has experience with that variety.
🍄💙🍄
|
cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
|
Re: Official Copelandia/Panaeolus Thread [Re: uninc4life2010] 2
#28211880 - 03/02/23 11:29 PM (10 months, 22 days ago) |
|
|
I have some AL growing and it acted same as BVI, TX, and SA.
|
uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
|
Re: Official Copelandia/Panaeolus Thread [Re: cooleko]
#28211916 - 03/03/23 12:24 AM (10 months, 22 days ago) |
|
|
|
Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 4 days
|
Re: Official Copelandia/Panaeolus Thread [Re: cooleko]
#28212082 - 03/03/23 07:01 AM (10 months, 22 days ago) |
|
|
Quote:
cooleko said: I have some AL growing and it acted same as BVI, TX, and SA.
I had the same experience. It wasn't the best producer for me though. It looked and grew about the same as the rest but produced less impressive flushes. I've seen JOC grow some killer flushes. I probably just didn't grow out enough cultures of it.
|
YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,208
Loc: Castles made of sand
Last seen: 18 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 2
#28212156 - 03/03/23 08:04 AM (10 months, 22 days ago) |
|
|
|
Rad Aghast



Registered: 12/07/18
Posts: 133
Loc: Minnesota
Last seen: 4 months, 29 days
|
Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 7
#28212229 - 03/03/23 09:03 AM (10 months, 22 days ago) |
|
|
Quote:
ruawakeyet said:
Quote:
Adas said: Where did you get the idea that Pans are not dung-loving? Cyans grow straight out of it.
JakeonCid has said he thinks they are really more grass-loving than dung-loving.
Not an either/or guy but it kinda makes sense to me actually. They typically inhabit grassland ecosystems disturbed by ungulate species - dung being processed grass.
On a lighter note - I have a deep spiritual connection to owls. I almost lost my shit when I looked at this T1 of Nec-D the other day.
|
|