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Egon_Spengler
Ever lurkin never workin



Registered: 04/02/22
Posts: 2,294
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Re: Official Copelandia/Panaeolus Thread [Re: Ratatoskr]
#28208368 - 02/28/23 04:59 PM (10 months, 25 days ago) |
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Quote:
Ratatoskr said:
if that is from my give away i had some weird growth on some plates for sure. i went for the wispy stuff, i usually do four plates at a time from a spore print. after about three sets of germination i had six plates set aside with some similar growth, not as universal on the plate but i did see some of that going on. the weird plates never contaminated but, still, weird looking.
They are, indeed! I’ll set up the SAB and pull some transfers. Thanks again.
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: craftymu]
#28208743 - 02/28/23 09:04 PM (10 months, 24 days ago) |
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Quote:
craftymu said: I am thinking this is metabolite but may be bad, any insight? These are Pan Cyan cased about 6 days ago.
 
I have never seen such a dense growth cross the bottom surface like that before. Doesn't look good IMO. The yellow liquid could be metabolites or just color leaching from the substrate but looking at that growth I would lean towards metabolites. It's bad looking really.
What does the surface look like? What substrate? Have you used agar?
Wait, is that green in on the right photo along the right side?
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: ReverendMyc] 2
#28208765 - 02/28/23 09:17 PM (10 months, 24 days ago) |
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Quote:
ReverendMyc said: Trying to keep my post count under 1000 until my international and non-machinable stamps arrive for a market place give away. But I would like some input on these T1s.
 BVI and Bisporus. First time with pans and planning to try a tub grow a la Baba Yaga. Anything to look for or try to avoid in pan myc selection? I see fast and whispy in the post above. These look pretty uniform in speed for each variety, but different from each other, and not necessarily whispy.
Agar recipe: 7g lme, 8g agar, 400 distilled water.
Those are all great looking plates. I would send all of those.
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Scotay
The Mad Mush Guy

Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 17 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#28208862 - 02/28/23 11:05 PM (10 months, 24 days ago) |
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Happy hump day friends and neighbors! I like all these people piling in here and sharing their dealings with growing the ancient fruit. I hope everybody is having a great week!! Peace to all you panheads!! 😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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karri0n
Mind Traveller



Registered: 08/29/20
Posts: 698
Last seen: 16 days, 4 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28208911 - 03/01/23 12:02 AM (10 months, 24 days ago) |
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Quote:
Baba Yaga said:
Quote:
craftymu said: I am thinking this is metabolite but may be bad, any insight? These are Pan Cyan cased about 6 days ago.
 
I have never seen such a dense growth cross the bottom surface like that before. Doesn't look good IMO. The yellow liquid could be metabolites or just color leaching from the substrate but looking at that growth I would lean towards metabolites. It's bad looking really.
What does the surface look like? What substrate? Have you used agar?
Wait, is that green in on the right photo along the right side?
It looks like green all over the place on both tubs to me.
Very dark green toward the edges. I think this is all mold, sorry to say.
--------------------
Panaeolus Bisporus
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craftymu
Stranger

Registered: 02/13/23
Posts: 16
Last seen: 7 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28209128 - 03/01/23 07:55 AM (10 months, 24 days ago) |
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Quote:
Baba Yaga said:
Quote:
craftymu said: I am thinking this is metabolite but may be bad, any insight? These are Pan Cyan cased about 6 days ago.
 
Quote:
I have never seen such a dense growth cross the bottom surface like that before. Doesn't look good IMO. The yellow liquid could be metabolites or just color leaching from the substrate but looking at that growth I would lean towards metabolites. It's bad looking really.
Me either thought this was the sign of strong growth in the beginning I was really hoping this time would be magical. I grew some treasure coast and Malabar and both of the cube grows were weird, the Malabar grow produced a crap ton of fruits and most of them were mutated. Treasure Cost had a bout of cobweb mold and produced few fruits that were very stunted. I am going to break down and scrub my grow area.
Quote:
What does the surface look like?
 
I think this is major overlay
Quote:
What substrate?
I am using a mix of some stuff I bought from Midwest Grow Kits, grass lovers mixed with their hpoo and bulk substrate. The casing is the peat moss they send with the grass lovers. I have been reading your tek though and will be switching to this next time for the pans and making my own cube substrate moving forward.
Quote:
Have you used agar?
No I have had little luck with agar my plates contaminate, I have tried everything from SAB and I am super clean I work on a super clean environment that is scrubbed clean and use a ton of iso gloves over those plastic gloves that you use to shove your arm in cows etc. lol!!
I do have a flow hood now and my neighbor is a professor in organic chemistry and has agreed to show me how to do agar properly. I have been buying empty plates off of Amazon and she thinks that they are probably in need some quality time in the autoclave and wants to look at my technique.
To answer your question, I have had a lot of success using LC I have heard it is best to go from agar to LC or at least do LC to agar then spawn but I have been doing this successfully for about a year now and have healthy LC by the pint for most of my MSS. Golden Teachers to APE.
I do want to work with plates though and my investment over time I am hoping will pay out. I am actually more interested in agar because I can grow and not have to eat the fruits, as it is I am having a hard time eating all them damn shrooms on my own. lol If I have anymore fruits I am going to have to start dosing unsuspecting people just so I do not waste them. This is of course a joke I would not dose anyone without them knowing...
Quote:
Wait, is that green in on the right photo along the right side?
No it is the lighting I see what you are saying but in person it is yellow. That said I am done and moving on to my next grow. looking at the JCM tek and moving my mushies out of my grow tent so I can plant some cannabis for summer.
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craftymu
Stranger

Registered: 02/13/23
Posts: 16
Last seen: 7 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: karri0n]
#28209141 - 03/01/23 08:04 AM (10 months, 24 days ago) |
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Yeah I see it in the picture but in person it is yellow, I am giving up and moving on to new trays.
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: craftymu]
#28209262 - 03/01/23 09:37 AM (10 months, 24 days ago) |
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I bought some glass petri dishes off Amazon, but I mostly use glad mini round containers. There's an awesome tek pasty whites easy agar. There are also those small condiment containers with little snap on lids, they can be put into the pressure cooker too. That tek pasty whites easy agar shows a no poor method. I like to start them off in that usually and than transfer to my nice glass plates.
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brshroomer
Moss bear hunter



Registered: 12/10/06
Posts: 970
Last seen: 9 months, 27 days
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Re: Official Copelandia/Panaeolus Thread [Re: craftymu]
#28209392 - 03/01/23 11:48 AM (10 months, 24 days ago) |
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If your agar is getting contam and the tub looks weird but your technique is on point I would check your sterilization procedure.
You are pc'ing agar for how long? Do the plates come sealed in the sleeves?
But also
Are you letting the SAB sit for a while to settle contams before using? Like half an hour?
Are you using a face mask?
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: craftymu] 1
#28209396 - 03/01/23 11:51 AM (10 months, 24 days ago) |
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Quote:
craftymu said: I grew some treasure coast and Malabar and both of the cube grows were weird, the Malabar grow produced a crap ton of fruits and most of them were mutated. Treasure Cost had a bout of cobweb mold and produced few fruits that were very stunted. I am going to break down and scrub my grow area.
[...] I have had little luck with agar my plates contaminate, I have tried everything from SAB and I am super clean I work on a super clean environment that is scrubbed clean and use a ton of iso gloves over those plastic gloves that you use to shove your arm in cows etc. lol!!
I do have a flow hood now and my neighbor is a professor in organic chemistry and has agreed to show me how to do agar properly. I have been buying empty plates off of Amazon and she thinks that they are probably in need some quality time in the autoclave and wants to look at my technique.
To answer your question, I have had a lot of success using LC I have heard it is best to go from agar to LC or at least do LC to agar then spawn but I have been doing this successfully for about a year now and have healthy LC by the pint for most of my MSS. Golden Teachers to APE.
I do want to work with plates though and my investment over time I am hoping will pay out. I am actually more interested in agar because I can grow and not have to eat the fruits, as it is I am having a hard time eating all them damn shrooms on my own. lol If I have anymore fruits I am going to have to start dosing unsuspecting people just so I do not waste them. This is of course a joke I would not dose anyone without them knowing...
So you are having some success with MSS and some trouble with it as well which is the nature of this practice and as good as it can get. Scrubbing your grow area and using all the ISO in thew world won't improve much if you keep sticking to using MSS to LC or spawn. It's great that you are going to learn agar properly because if have bought a flow hood and continue to use MSS to LC I would have to tell you that you are throwing your money at the wrong things like a moron. I am sure it will click soon and you will realize that agar is actually easy to work with. Just be careful what you tell and show that professor. Don't let anyone know you are growing actives.
Get a box of sterile plates. I use condiment cups which are awesome and cheap. Keep doing agar
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brshroomer
Moss bear hunter



Registered: 12/10/06
Posts: 970
Last seen: 9 months, 27 days
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 2
#28209545 - 03/01/23 01:45 PM (10 months, 24 days ago) |
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Just some pics of my first Pan cyan grow ever.
Wild print Spore syringe to wheat (wanted to select from the largest pheno pool)

Spawned to cowpoo/coir 50/50 at 1:2

Planning on casing soon...impressed that these spores were injected day 14...jars are 500ml, but I thought it was pretty fast
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craftymu
Stranger

Registered: 02/13/23
Posts: 16
Last seen: 7 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28209831 - 03/01/23 05:01 PM (10 months, 24 days ago) |
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LMAO
Quote:
Baba Yaga said: if have bought a flow hood and continue to use MSS to LC I would have to tell you that you are throwing your money at the wrong things like a moron
Well said!! I am picking up what you are putting down!! 
I have had about 95% success with MSS/Spawn and MSS/LC/Spawn in regards to cubes. I think that has more to do with how forgiving cubes are than me. Also the grows are simple and done using teks learned from here. I am just a student who has gotten lucky.
I have used the cups also as I like the idea of using something that is not as obvious.
Quote:
Baba Yaga said: Just be careful what you tell and show that professor
I live in a unique place where these are no longer illegal. I think I will revisit agar now that I have an autoclave and flow hood and give it a go without outside help.
Thank you Baba Yaga and all of shroomery for your time and input.
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uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
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Re: Official Copelandia/Panaeolus Thread [Re: craftymu]
#28209905 - 03/01/23 05:36 PM (10 months, 24 days ago) |
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Can someone recommend a good Pan bulk substrate for loading into spawn bags? I plan on using Asura's method, but it's very manure heavy, and manure isn't as easy for me to get ahold of. I'd also like to avoid using vermiculite due to cost reasons. Plus, I don't like having an addition ingredient that I need to keep stock of. I was considering the following recipes for spawn bags:
50% horse manure 25% coir 25% hydrated oats Handful of gypsum
or
33% horse manure 33% coir 33% hydrated oats Handful of gypsum
Would either of these work for sterilized spawn bags?
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: uninc4life2010]
#28210308 - 03/01/23 09:27 PM (10 months, 23 days ago) |
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My understanding is that you don't want to sterilize but pasturize by keeping it at a constant temp for 2hrs between 150 to 160 or 160 to 170, the guy in the video quoted both.lol. there is a tek where you use a heavy duty bin, a thermometer on the lid that the needle or spike goes through. A high temp hose that goes from the PC to the lid, bulk sub is in a heavy duty garbage bag tied off, lid goes on and needle goes into substrate giving temp. You can YouTube diy bulk pasteurization.
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uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
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Re: Official Copelandia/Panaeolus Thread [Re: abductee]
#28210346 - 03/01/23 09:53 PM (10 months, 23 days ago) |
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Quote:
abductee said: My understanding is that you don't want to sterilize but pasturize by keeping it at a constant temp for 2hrs between 150 to 160 or 160 to 170, the guy in the video quoted both.lol. there is a tek where you use a heavy duty bin, a thermometer on the lid that the needle or spike goes through. A high temp hose that goes from the PC to the lid, bulk sub is in a heavy duty garbage bag tied off, lid goes on and needle goes into substrate giving temp. You can YouTube diy bulk pasteurization.
You absolutely can sterilize bulk substrate. You PC it just like grain spawn and inoculate just like grain spawn. Then, you wait for it to colonize, break it up into trays or monos, let the colonized bulk substrate recover for a few days, then case with pasteurized medium and fruit as normal. This removes the need to pasteurize, cool, add colonized spawn, wait for colonization, then case and fruit.
Just check out Asura's detailed write-up and it'll make more sense.
https://www.shroomery.org/forums/showflat.php/Number/25987049
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dowodenum
Noob Pope



Registered: 08/05/19
Posts: 1,268
Loc: 🇨🇦
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Re: Official Copelandia/Panaeolus Thread [Re: uninc4life2010]
#28210361 - 03/01/23 10:11 PM (10 months, 23 days ago) |
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MudaFuka's bottle tek also involves sterilization of grains+bulksub. 8 years old but people still make it work, and well. Good reading!
--------------------
 🅃 🄴 🄰 🄼 🄲 🅄 🅁 🄸 🄾 🅄 🅂
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karri0n
Mind Traveller



Registered: 08/29/20
Posts: 698
Last seen: 16 days, 4 hours
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Re: Official Copelandia/Panaeolus Thread [Re: uninc4life2010] 2
#28210364 - 03/01/23 10:12 PM (10 months, 23 days ago) |
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Quote:
uninc4life2010 said: Can someone recommend a good Pan bulk substrate for loading into spawn bags? I plan on using Asura's method, but it's very manure heavy, and manure isn't as easy for me to get ahold of. I'd also like to avoid using vermiculite due to cost reasons. Plus, I don't like having an addition ingredient that I need to keep stock of. I was considering the following recipes for spawn bags:
50% horse manure 25% coir 25% hydrated oats Handful of gypsum
or
33% horse manure 33% coir 33% hydrated oats Handful of gypsum
Would either of these work for sterilized spawn bags?
I'd leave out the coir and use straw or hay if you are going to be sterilizing the whole thing.
10-15% grain is also probably better for panaeolus than 25%.
If you are pasteurizing, you can't use any grain in the mix and you will need to spawn colonized grain to the pasteurized substrate.
As for manure, I've had success using Scott's brand manure/humus blend for gardens. Black Kow never worked out for me but the Scotts stuff has worked well.
--------------------
Panaeolus Bisporus
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Scotay
The Mad Mush Guy

Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 17 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28210379 - 03/01/23 10:24 PM (10 months, 23 days ago) |
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Quote:
Baba Yaga said:
Quote:
craftymu said: I grew some treasure coast and Malabar and both of the cube grows were weird, the Malabar grow produced a crap ton of fruits and most of them were mutated. Treasure Cost had a bout of cobweb mold and produced few fruits that were very stunted. I am going to break down and scrub my grow area.
[...] I have had little luck with agar my plates contaminate, I have tried everything from SAB and I am super clean I work on a super clean environment that is scrubbed clean and use a ton of iso gloves over those plastic gloves that you use to shove your arm in cows etc. lol!!
I do have a flow hood now and my neighbor is a professor in organic chemistry and has agreed to show me how to do agar properly. I have been buying empty plates off of Amazon and she thinks that they are probably in need some quality time in the autoclave and wants to look at my technique.
To answer your question, I have had a lot of success using LC I have heard it is best to go from agar to LC or at least do LC to agar then spawn but I have been doing this successfully for about a year now and have healthy LC by the pint for most of my MSS. Golden Teachers to APE.
I do want to work with plates though and my investment over time I am hoping will pay out. I am actually more interested in agar because I can grow and not have to eat the fruits, as it is I am having a hard time eating all them damn shrooms on my own. lol If I have anymore fruits I am going to have to start dosing unsuspecting people just so I do not waste them. This is of course a joke I would not dose anyone without them knowing...
So you are having some success with MSS and some trouble with it as well which is the nature of this practice and as good as it can get. Scrubbing your grow area and using all the ISO in thew world won't improve much if you keep sticking to using MSS to LC or spawn. It's great that you are going to learn agar properly because if have bought a flow hood and continue to use MSS to LC I would have to tell you that you are throwing your money at the wrong things like a moron. I am sure it will click soon and you will realize that agar is actually easy to work with. Just be careful what you tell and show that professor. Don't let anyone know you are growing actives.
Get a box of sterile plates. I use condiment cups which are awesome and cheap. Keep doing agar 
Baba..... I took use condiment cups!! They are cheap and if they contam they just whole hog get tossed out. They've been sterile out of the package for me. I use a SAB for pouring plates and/or doin grain jars. It's served me ok. Hopefully I'll have some pans of my own in the near future!! Happy Thursday ya panheads!! 😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
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Re: Official Copelandia/Panaeolus Thread [Re: karri0n]
#28210394 - 03/01/23 10:38 PM (10 months, 23 days ago) |
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Quote:
karri0n said:
Quote:
uninc4life2010 said: Can someone recommend a good Pan bulk substrate for loading into spawn bags? I plan on using Asura's method, but it's very manure heavy, and manure isn't as easy for me to get ahold of. I'd also like to avoid using vermiculite due to cost reasons. Plus, I don't like having an addition ingredient that I need to keep stock of. I was considering the following recipes for spawn bags:
50% horse manure 25% coir 25% hydrated oats Handful of gypsum
or
33% horse manure 33% coir 33% hydrated oats Handful of gypsum
Would either of these work for sterilized spawn bags?
I'd leave out the coir and use straw or hay if you are going to be sterilizing the whole thing.
10-15% grain is also probably better for panaeolus than 25%.
If you are pasteurizing, you can't use any grain in the mix and you will need to spawn colonized grain to the pasteurized substrate.
As for manure, I've had success using Scott's brand manure/humus blend for gardens. Black Kow never worked out for me but the Scotts stuff has worked well.
Question: Why use straw if there is already a lot of it still in the horse manure? Is it to aerate the manure better? Does P. Cyanescens use the straw efficiently as a food source?
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: uninc4life2010]
#28210600 - 03/02/23 06:00 AM (10 months, 23 days ago) |
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Thanks, that sounds good, I'll check it out.
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