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Invisiblecooleko
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Re: Official Copelandia/Panaeolus Thread [Re: mikeybox]
    #28204837 - 02/26/23 12:41 AM (10 months, 27 days ago)

All my tubs and shoeboxes have been from spores and I've seen a lot of wall to wall flushes with my BVI. So I have no interest in cloning until that changes.


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Offlineminameisjake
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 4
    #28204866 - 02/26/23 01:44 AM (10 months, 27 days ago)

This is my first ever harvest, I underestimated how stringy these fruits would be after drying out. Do they look normal? I feel like they are going to be a pile of dust in no time. I’m now thinking it will be easier to blend them into a powder for storage. Is that a common way to deal with mushrooms this small/fragile?


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: minameisjake]
    #28204881 - 02/26/23 02:36 AM (10 months, 27 days ago)

Good job!

I just stuff dried fruits in a jar. Vacuum seal them for long long term storage.

How did you grow these?


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Invisiblecooleko
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Re: Official Copelandia/Panaeolus Thread [Re: minameisjake]
    #28204895 - 02/26/23 03:25 AM (10 months, 27 days ago)

Second Baby Yaga's advice. I just stuff them in jars. Don't worry about packing them densely, you will have enough that you need to push them down until they won't compact anymore.

I have made pills out of some and the people who tried them say they held their potency well.

Others who stored the powder have reported no discernable loss even after 5 years, so do whatever works for you!


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Offlinemikeybox
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Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg] * 1
    #28205090 - 02/26/23 08:37 AM (10 months, 27 days ago)

So do you think I should clone a large fruit even if it's not the biggest I've had, do you still think it's worth it? Or do you think I'm better off waiting for a more outstanding jumbo fruit and cloning that?

I had a really impressive jumbo fruit in my first flush that I tried to clone, but sadly the clone didn't take due to bacteria.  It was the fruit in my profile pic.  I wish I had been able to clone it, it was huge for a pan!  Next time I will try it with either water agar or antibiotics agar.

My dream would be a whole flush of pans that are big enough to be confused with cubes :evil:


Edited by mikeybox (02/26/23 09:06 AM)


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: mikeybox] * 6
    #28205182 - 02/26/23 10:04 AM (10 months, 27 days ago)

I think cloning your current best fruit is still worth it. Here's an anecdote from my limited experience:

From my first successful pan grow, (novelty grow), I cloned the largest fruit here:

Novelty grow- my first Pan Cyans ever!

That culture produced the closest I ever got to a panopy, even though the fruits were still rather small:

My first clone culture tray of PHV - small fruits but the closest I ever came to a canopy. (A lot of beginner's luck!)

I didn't keep cloning more fruits, but I did keep transferring to new plates to keep the culture running, and some of the culture was used for A2G. The culture started sectoring on agar, so I tried taking as many of them as I could manage for future A2G.

Some of those cultures started producing some large fruits, even though I never did get canopies. (IMO, I think my spawn wasn't clean enough to deliver canopies, as I lost a bunch of trays to trich.) Here are some examples of the fruits that came from that original clone culture, after several transfers:





My personal record for largest PHV

If I ever get up and running again, I will not take so many transfers. If I find a culture that produces some large fruits again, I will find another method to expand it at that point, and try to stop any further isolation.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 4
    #28205359 - 02/26/23 12:13 PM (10 months, 27 days ago)

Cloning is worth it IME. Often it will not give you an exact copy of the fruit you took tissue from but it will give you more uniform fruits and flushes with a tendency to grow similar to the mother fruit. But that is not the only reason, when growing out clones you take away a good deal of variability which enables you to experiment with better results especially when only doing small sample sizes. It helps with understanding a certain species cause it can show you what is genetic and what environmental when growing the same clone cultures repeatedly. It really depends on where you want to go and what floats your boat. I like cloning because I like to experiment, it gives me more control and adds consistency, another aspect of the hobby that keeps it interesting.


Edited by Baba Yaga (02/26/23 12:40 PM)


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InvisibleSeeker604
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 3
    #28205491 - 02/26/23 01:16 PM (10 months, 27 days ago)

For what it's worth, Jake Oncid said in his Pans class that the majority of success with Pans species rests on the culture selection you make. He said running a culture taken from a clone that has proven it can survive your local conditions and methods will give you better, more consistent results than growing from MS each time. He said unlike other species, Pans are very "wimpy" when it comes to competing with bacteria and other contaminations, so we should always choose the fastest/healthiest culture from our germ plates, run them, note which culture survived the grain colonization (some cultures he said will fail after the first shake), and then select clones from your best trays at the end of the grow.

Peace & Light,

Seeker604


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Seeker604] * 3
    #28205558 - 02/26/23 01:44 PM (10 months, 27 days ago)

I had read his write up years ago and forgot about most of it cause I do tubs, only thing that stuck with me was his pasteurization method. Now after growing mostly pans for another 3 years I came full circle to the same conclusion. To clone what is thriving in your conditions and produce clean cultures is key. Nothing new at all, it's just of higher importance when working with pans compared to a more robust species like cubes.

Not agreeing with the shake test though, that alone does not prove 100% that culture health is compromised IMO. Especially not the case when shaking close to or at full colonization.


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InvisibleSeeker604
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28206020 - 02/26/23 07:53 PM (10 months, 26 days ago)

Quote:

Baba Yaga said:
Not agreeing with the shake test though, that alone does not prove 100% that culture health is compromised IMO. Especially not the case when shaking close to or at full colonization.




To clarify, he said that is one indicator of the health of the culture - not the defining one. He was talking about first shake at 30%. He recommended in the class to do two shakes: 30% and again at 80%. Second shake being to just help take 1-2 days off total colonization time.

Once I'm ready to go I'll be using my gourmet Martha (I'll be adding an exhaust fan in how he does the tent build) but also going to attempt with monotubs as well since that's what I'm most accustomed to using with cubes. He stressed the point of being fluid and knowing how to monitor and tweak the fogging/evaopartion cycle in the tent regardless of how you do the build, and to make sure you dial in the time of the cycle to ensure not drying out your trays. He said that once the trays become dry, there's no way to bring it back, but that if your fogger is adding too much water we can just empty the trays and tweak fogger run time and how long the exhaust fan runs after fogger turns off.

Really looking forward to get going in two more months. <3


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Seeker604] * 2
    #28206332 - 02/27/23 03:02 AM (10 months, 26 days ago)

@seeker

This is Hindsights tent, he explains everything very well. It might help you: https://www.shroomery.org/forums/showflat.php/Number/27993602


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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OfflineSingularFusion
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Re: Official Copelandia/Panaeolus Thread [Re: Seeker604]
    #28206333 - 02/27/23 03:05 AM (10 months, 26 days ago)

That's interesting Jake also talking about the failing after first shake, I must've missed that while reading somehow... that is pretty much my interpretation of what I have seen on my Nec-D and Goliath spawn for LAGM

it is still going, but man is it slow now. May have to start again with those from spore possibly I guess


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Offlineminameisjake
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 5
    #28206338 - 02/27/23 03:24 AM (10 months, 26 days ago)

Thanks! It took a few tries to get a decent grow, but I’d say I've been learning along the way. This attempt I grew them in a tent following Jake Oncid's JOC tek basically. I’m attempting to clone a few so I’m excited to see how that will turn out. It was difficult trying to get a clean piece of tissue from such a small stem.


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Invisibleabductee
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Re: Official Copelandia/Panaeolus Thread [Re: minameisjake] * 3
    #28206465 - 02/27/23 07:56 AM (10 months, 26 days ago)

Hi everyone,first time growing pan rainbow eucalyptus, and getting some good info from the thread. I swiped some spores on a plate Feb 18th and got some really good growth already.
I've grown cubes and atl#7, both with wbs and cvg. I feel these pans are going to expand my knowledge in more ways than one.lol


Edited by abductee (02/27/23 08:18 AM)


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InvisibleSeeker604
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28206511 - 02/27/23 08:57 AM (10 months, 26 days ago)

Quote:

Shrimps said:
@seeker

This is Hindsights tent, he explains everything very well. It might help you: https://www.shroomery.org/forums/showflat.php/Number/27993602




Hey Shrimps!

Hindsight and I have chatted previously and he shared the thread. I’m actually mimicking his set up more than Jake’s due to the space issues that I have. But I am using some of the gear Jake recommended.

πŸ„πŸ’™πŸ„πŸ’™


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InvisibleTucky
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Re: Official Copelandia/Panaeolus Thread [Re: Seeker604] * 7
    #28206657 - 02/27/23 11:40 AM (10 months, 26 days ago)

This is my first attempt at Pan’s and I followed the Gordo tek exactly. For three weeks now I have been getting close to this amount every morning.  Never a defined flush but just constantly producing fruit.



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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Tucky] * 2
    #28206750 - 02/27/23 12:42 PM (10 months, 26 days ago)

Gorgeous Pans Tucky! Awesome results. What variety is this?


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InvisibleTheDoobskerS
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28206816 - 02/27/23 01:26 PM (10 months, 26 days ago)

Beautiful grows everyone! :bow2:
Planning to take some clones of this tray if it looks good


Cased 48 hours ago and put straight into the jcm, currently sitting at about 76F.



Wondering if it looks dry though. I misted it too heavily after casing and ended up dumping out some of the water pooled at the bottom of the tray a couple hours after misting


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Invisibleabductee
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Re: Official Copelandia/Panaeolus Thread [Re: Tucky]
    #28206821 - 02/27/23 01:28 PM (10 months, 26 days ago)

I'll have to check that tek out. Thick looking fruit.


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InvisibleTucky
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 2
    #28206840 - 02/27/23 01:46 PM (10 months, 26 days ago)

Quote:

Adas said:
Gorgeous Pans Tucky! Awesome results. What variety is this?



Estero. Thanks!


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