Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: < First | < Back | 1428 | 1429 | 1430 | 1431 | 1432 | 1433 | 1434 | 1435 | 1436 | 1437 | 1438 | 1439 | 1440 | 1441 | 1442 | 1443 | 1444 | 1445 | 1446 | 1447 | 1448 | Next > | Last >
InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: mushroom_gnome] * 1
    #28193139 - 02/18/23 02:20 PM (11 months, 4 days ago)

Relative many estero grows are showing these weird deformed caps. 

here is just a couple:

https://www.shroomery.org/forums/showflat.php/Number/27793722#27793722
https://www.shroomery.org/forums/showflat.php/Number/28042510#28042510


This particular phenotype seems just to happen with estero, haven't seen it any other pan yet.

The darkening stems and caps more likely to be caused by additional contamination in my opinion rather than also by a genetic factor. IIRC the spawn for these was still inoculated with a syringe? So chances are high that contam is causing this.


Quote:

mushroom_gnome said:
Contam or something different? These pancakes colonized fantastic with no seeming signs of contam. Fruits in multiple jars are expressing in this way.









That's contam IMO. Not sure what exactly causes this but you could try keeping them dryer and see if new fruits are looking happier. See, if you can track it back in your cultures or if other grows from other plates/LC are doing the same.


Extras: Filter Print Post Top
Offlinemushroom_gnome
Stranger
 User Gallery

Registered: 06/30/22
Posts: 82
Last seen: 4 hours, 30 minutes
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28193177 - 02/18/23 02:42 PM (11 months, 4 days ago)

Hmmm.. I suspect my casing material. Time to re-pasteurize.


--------------------
Mystery Mushroom


Extras: Filter Print Post Top
Offlineold_fart_out_west
Stranger
 User Gallery

Registered: 01/27/23
Posts: 49
Last seen: 45 minutes, 1 second
Re: Official Copelandia/Panaeolus Thread [Re: mushroom_gnome]
    #28193209 - 02/18/23 02:53 PM (11 months, 4 days ago)

Thank you Adas and Baba Yaga! Really helpful information and context.

The two sources of fresh air in my martha are 1) the humidifier (placed outside the tent) and 2) slits cut in the tent. I'm going to experiment with a small computer fan, attached near the top, that pushes fresh air in, to see if that improves my fruits.


Extras: Filter Print Post Top
OfflineScotay
The Mad Mush Guy
Male User Gallery
Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 15 hours
Re: Official Copelandia/Panaeolus Thread [Re: old_fart_out_west]
    #28193845 - 02/18/23 10:24 PM (11 months, 3 days ago)

Quote:

old_fart_out_west said:
Thank you Adas and Baba Yaga! Really helpful information and context.

The two sources of fresh air in my martha are 1) the humidifier (placed outside the tent) and 2) slits cut in the tent. I'm going to experiment with a small computer fan, attached near the top, that pushes fresh air in, to see if that improves my fruits.



I'll be watching old fart!! I'm just glad there's people cool enough on here to share their knowledge and experiences.  Happy Sunday you panheads!! Peace and chicken grease!! 😎✌️


--------------------
Just chillin like a Villain and talking to like minded individuals!!
             


              Mush Love!!
                😎✌️


Extras: Filter Print Post Top
OnlineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,907
Loc: Rent free in your head
Last seen: 15 seconds
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Scotay] * 7
    #28194127 - 02/19/23 06:28 AM (11 months, 3 days ago)



Cyan huasteca RDU and bispo in rye and straw bottles. Cased with sterilezed peat/verm, no pH adjustment.

Cyan was g2g using colonized millet, took 6 days to colonize bottles. Bispo was A2G and took upwards of 20. They've both been consolidating for about a week now

Plus bispo in rye and coir bottles, A2G a week ago and shaken on day 4.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
OfflineTimestop413
Stranger
 User Gallery


Registered: 06/17/20
Posts: 294
Last seen: 30 days, 8 hours
Re: Official Copelandia/Panaeolus Thread [Re: SirPsycho] * 1
    #28194383 - 02/19/23 11:22 AM (11 months, 3 days ago)

Do these jars have too much condensation inside of them?  They still have a day or two left of colonizing.  Recovered within 24hours of shaking but I am concerned of bacteria or spreading bacteria if I spawn bad jars.  Pan Cyan Estero on rye inoculated with small ketchup cup (from spore print) on 2-2(17 days ago).



Edited by Timestop413 (02/19/23 11:26 AM)


Extras: Filter Print Post Top
InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Timestop413] * 1
    #28194448 - 02/19/23 12:16 PM (11 months, 3 days ago)

I would spawn this and keep a record in which tray or tub this went.

What I have found is that if you shake a jar which is close to full colonization then it can produce a lot of condensation from all the mycelium recovering all at once. Especially with pans where it is happening fast.

I don't see any bacterial wet spots in this jar. If it looked alright beforehand then it should be still alright.


Found an old post about this.

Quote:

Baba Yaga said:
Final update on the poorly recovered jars:

So just to recap I spawned these two jars which wouldn't recover growing out nice and fluffy but looked like this which often happens
for me when shaking a jar that is almost done.

Two weeks after last shake:



I was throwing jars like this out at the beginning but after a few of them I questioned whether this was actually contamination as there were
no signs of any contam before the shake, no mold, no creamy dense mycelium, no syrupy wet kernels or suspect looking bald spots. Yes they
do recover poorly and they look wet after the shake but there is a difference to bacterial wet and condensation wet IMO. The final push to
give them a go was reading a statement of member conicr saying that this happens sometimes but its no deal breaker. Now I spawn jars like
this whenever I get them and have no problems. Also sitting in the jar for 2 weeks doesn't seem to make a difference. I still do throw jars
out though just not when totally fine almost done jars recover poorly after a shake.

Both tubs received FAE during colonization and were put into full fruiting condition one day after full colonization. Temps plummeted
for a couple of nights while fruiting and went down to 68F in the room, I've got the heater going now.

First clone C4T3 which was a day behind but turned out to be an OK tub. This is also the least productive clone out of the 6 I got going.

236g wet. 1 myco quart @ a spawn ratio of ~1:2.5




Second clone C3T3, pretty good and I used the other style tub for this which I like only downside is you can't see through the plastic.

327g wet. 1 myco quart @ a spawn ratio of ~1:3.5



If you have a jar like this and there was no sign of contamination before the shake then try spawning it. You might be surprised
and I sincerely hope it will be in a good way.

Edit: update photo and weight




Extras: Filter Print Post Top
OfflineTimestop413
Stranger
 User Gallery


Registered: 06/17/20
Posts: 294
Last seen: 30 days, 8 hours
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28194484 - 02/19/23 12:44 PM (11 months, 3 days ago)

Quote:

Baba Yaga said:
I would spawn this and keep a record in which tray or tub this went.

What I have found is that if you shake a jar which is close to full colonization then it can produce a lot of condensation from all the mycelium recovering all at once. Especially with pans where it is happening fast.

I don't see any bacterial wet spots in this jar. If it looked alright beforehand then it should be still alright.





Ok thanks, I will send it.

I'm also going to make some water agar plates.  I see Hindsight mentioning using PF jars to clean things up, would water agar basically do the same thing without the added step of having to make pf jars?


Edited by Timestop413 (02/19/23 12:46 PM)


Extras: Filter Print Post Top
InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Timestop413]
    #28194493 - 02/19/23 12:56 PM (11 months, 3 days ago)

Water agar is working really well. I like to let the sample grow out a bit more like 30mm in diameter and then go back to regular. I have poked straight to LC too but doing the extra step of checking on regular agar feels better.


Extras: Filter Print Post Top
Offlinepsilocybist
All is One
Male User Gallery


Registered: 05/22/14
Posts: 528
Loc: Far
Last seen: 1 day, 11 hours
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 4
    #28194496 - 02/19/23 12:57 PM (11 months, 3 days ago)


Same panaelous cyanescens goliath, germinated from 8yr old spore print.
Now cleaned on agar.
Will make a reliable LC out of this plate.

Also, just not to waste other germinated spores (which were directly injected into nutrient lc media, and showed fast growing mycelium in a week, no off smells, and liquid was clearest with floating mycelium) last night I inoculated 25 buckets of properly pasteurized coco+peat+urea+straw substrate, using those LC bottles.
I like this gamble. No big loss, but surely the win will be big. (Each bucket holds around half liter substrate.)


Extras: Filter Print Post Top
OfflineAdas
Lonely Dreamer
Male User Gallery


Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 43 minutes, 40 seconds
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: psilocybist] * 1
    #28194594 - 02/19/23 02:18 PM (11 months, 3 days ago)

8 years.. holy cow :ohwow:


Extras: Filter Print Post Top
Offlinemikeybox
Stranger
 User Gallery

Registered: 04/19/20
Posts: 34
Last seen: 10 months, 25 days
Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #28194618 - 02/19/23 02:33 PM (11 months, 3 days ago)

So I'm currently fruiting pan cambo Goliath in a humidity tent in aluminum cake tins.

They're growing the second flush now, and the substrate has shrunk significantly.  I'm thinking it could use some water.

Is bottom watering safe to do with pans?  Can I dump water in the tin so that the substrate is floating in water for a few hours, to rehydrate it?

Or is that a risky move?


Extras: Filter Print Post Top
OfflineAdas
Lonely Dreamer
Male User Gallery


Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 43 minutes, 40 seconds
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: mikeybox]
    #28194636 - 02/19/23 02:37 PM (11 months, 3 days ago)

Bottom watering is fine but I'd personally focus more on keeping the casing wet.


Extras: Filter Print Post Top
OfflineTimestop413
Stranger
 User Gallery


Registered: 06/17/20
Posts: 294
Last seen: 30 days, 8 hours
Re: Official Copelandia/Panaeolus Thread [Re: mikeybox]
    #28194783 - 02/19/23 04:13 PM (11 months, 3 days ago)

I noticed my trays would shrink quite a bit as well, wasn't sure how to keep them from doing it.

Also, Baba Yaga I know you just referenced some of those jars not recovering the best but if jars are recovering..do any of you grain to grain transfer with pans or do you individually inoculate each jar you are going to spawn with agar or LC?


Edited by Timestop413 (02/19/23 05:08 PM)


Extras: Filter Print Post Top
InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Timestop413]
    #28195280 - 02/19/23 08:58 PM (11 months, 2 days ago)

Did grain to grain a few times. It works like with any other mushroom.

I am running more smaller batches for the last years and use LC for everything. Have a lot of 100 and 250 ml jars and use those to inoculate 2-5 jars so I can run a variety of different genetics. Would only do g2g again if I wanted to produce a shitload of pans with good performing clones but that's not my goal. I am more into having fun with experiments and throw out most of the stuff I grow.


Extras: Filter Print Post Top
InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: mikeybox] * 1
    #28195290 - 02/19/23 09:03 PM (11 months, 2 days ago)

Quote:

mikeybox said:
So I'm currently fruiting pan cambo Goliath in a humidity tent in aluminum cake tins.

They're growing the second flush now, and the substrate has shrunk significantly.  I'm thinking it could use some water.

Is bottom watering safe to do with pans?  Can I dump water in the tin so that the substrate is floating in water for a few hours, to rehydrate it?

Or is that a risky move?




I bottom water but only if a good pin set is present and the fruits are already 2 inch or so tall, that is when they really start taking off and suck up a lot of water.




Quote:

psilocybist said:

Same panaelous cyanescens goliath, germinated from 8yr old spore print.
Now cleaned on agar.
Will make a reliable LC out of this plate.

Also, just not to waste other germinated spores (which were directly injected into nutrient lc media, and showed fast growing mycelium in a week, no off smells, and liquid was clearest with floating mycelium) last night I inoculated 25 buckets of properly pasteurized coco+peat+urea+straw substrate, using those LC bottles.
I like this gamble. No big loss, but surely the win will be big. (Each bucket holds around half liter substrate.)





What is the urea for? That is the first time I'm hearing about it being used. Nitrogen source?


Extras: Filter Print Post Top
Offlinepsilocybist
All is One
Male User Gallery


Registered: 05/22/14
Posts: 528
Loc: Far
Last seen: 1 day, 11 hours
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28195377 - 02/19/23 10:34 PM (11 months, 2 days ago)

Yes, a natural source for nitrogen.
It doesn't shift pH of media, and all my agar preparations contain a sub-percent amount of urea.
Plus, since pancyans are dung-loving species, urea wouldn't be toxic to them at all.
Stamet lists urea as a ingredient for substrates as well, (composted)


Extras: Filter Print Post Top
OfflineScotay
The Mad Mush Guy
Male User Gallery
Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 15 hours
Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
    #28195402 - 02/19/23 10:59 PM (11 months, 2 days ago)

Quote:

psilocybist said:

Same panaelous cyanescens goliath, germinated from 8yr old spore print.
Now cleaned on agar.
Will make a reliable LC out of this plate.

Also, just not to waste other germinated spores (which were directly injected into nutrient lc media, and showed fast growing mycelium in a week, no off smells, and liquid was clearest with floating mycelium) last night I inoculated 25 buckets of properly pasteurized coco+peat+urea+straw substrate, using those LC bottles.
I like this gamble. No big loss, but surely the win will be big. (Each bucket holds around half liter substrate.)



Wowww brother....8 years.... That's amazing!!! Just goes to show how resilient these little beasts are right!?!? Makes me hopeful for some of the prints I have. I do have a Goliath print. I'll just be happy when I get my first pansπŸ’― I hope everybody had a pantastic weekend. Happy Monday to y'all friends and neighbors. 😎✌️


--------------------
Just chillin like a Villain and talking to like minded individuals!!
             


              Mush Love!!
                😎✌️


Extras: Filter Print Post Top
Offlinepsilocybist
All is One
Male User Gallery


Registered: 05/22/14
Posts: 528
Loc: Far
Last seen: 1 day, 11 hours
Re: Official Copelandia/Panaeolus Thread [Re: Scotay] * 2
    #28195581 - 02/20/23 04:06 AM (11 months, 2 days ago)

Well I wasn't so hopeful about this spore prints too. They're certainly older than 8 yrs, cuz I received them in a trade here, in 2015. God knows how old they already were back then.

Also have a Semperviva mycelium going, this also sat in my fridge for 8 years, but not as a spore print. It was a spore syringe, which when I took out of fridge, inside the syringe, the spores were turned into a small dark floating globe :shrug:
So I dumped out almost all the water, and then using a needle poked into the little globe, it kinda fell apart immediately. Then I poked the spore-bearing needle into agar. First petri was clean AF. Though I transferred twice.
This is T2:


That little colony @ 6 o'clock is bacillius. But I've seen Semperviva mycelium running over such colonies like tanks go over toy cars
:takeout:


Extras: Filter Print Post Top
Invisibleekabpils
Exploring Exotics
 User Gallery


Registered: 10/20/21
Posts: 167
Re: Official Copelandia/Panaeolus Thread [Re: Timestop413]
    #28195615 - 02/20/23 05:21 AM (11 months, 2 days ago)

Hi Baba,

the main drawback of placing it just after the spawn is that mushrooms grow on the side a lot. Some of them will stay small and will never grow further. This is the waste of substrate nutritious resourses. I see it on your first picture and had the same in mine. I never had this when used the more common approach to wait until the substrate will taken and only then place it for fruiting.


--------------------


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 1428 | 1429 | 1430 | 1431 | 1432 | 1433 | 1434 | 1435 | 1436 | 1437 | 1438 | 1439 | 1440 | 1441 | 1442 | 1443 | 1444 | 1445 | 1446 | 1447 | 1448 | Next > | Last >

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* The official Psilocybe natalensis thread
( 1 2 3 4 ... 515 516 )
Hillbillyjohn 328,218 10,313 01/29/24 04:10 PM
by Smellit
* Official Misc EXOTIC Thread
( 1 2 3 4 ... 132 133 )
Jakeoncid419 110,077 2,649 01/29/24 03:16 PM
by SirPsycho
* Rye-Z-Morph Bag for Panaeolus (Copelandia) Cyanescens? rob_531 4,313 12 01/10/05 12:33 PM
by agar
* Copelandia "Pan" Cyanescens sancho 4,962 6 09/07/07 10:00 AM
by suave
* Re: look at these copelandia cyanescens they are beautiful Anonymous 5,161 2 12/25/99 07:16 AM
by sean123
* which tek for copelandia cyanescens (spore print)? FfF 7,484 10 08/26/04 05:44 AM
by hawksapprentice
* Hawaiian Copelandia Grow Kit - how much more difficult are they? GabrielKnight 8,125 5 01/03/05 04:59 PM
by ticktock
* Preparing jars for Copelandia? PawPaw 1,195 14 04/02/05 01:11 PM
by PawPaw

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
924,103 topic views. 26 members, 213 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.042 seconds spending 0.021 seconds on 15 queries.