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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 3
    #28133782 - 01/10/23 02:03 PM (1 year, 19 days ago)

If I manage to fruit the Ambrose culture again, I'd definitely like to share some spores around so that you guys can test them or send them for testing. It still blows my fuckin mind how something can be so potent.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28133820 - 01/10/23 02:30 PM (1 year, 19 days ago)

Quote:

Adas said:
Ambrose





First time I'm hearing this name, what is it and where is it from?


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InvisibleGuerrilla
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 3
    #28133827 - 01/10/23 02:34 PM (1 year, 19 days ago)

Quote:

Baba Yaga said:
Quote:

Adas said:
Ambrose





First time I'm hearing this name, what is it and where is it from?




Ambrose is a G.

https://www.shroomery.org/forums/showprofile.php?User=489972


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Being pissed on does not make you a real man.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Guerrilla]
    #28133839 - 01/10/23 02:39 PM (1 year, 19 days ago)

:whathesaid:


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OfflineFunnyFungiName
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28133847 - 01/10/23 02:46 PM (1 year, 19 days ago)

Anyone tried to germ on brf pucks? What were your results? I saw it mentioned in the Josex Biopsy thread that these work well for pans.

Ive tried several times with pans while I was still struggling with cubes and never got anything to grow so now that Im a bit more experienced Im making another attempt to germ some pans on agar and also some brf pucks.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: FunnyFungiName] * 2
    #28133859 - 01/10/23 02:54 PM (1 year, 19 days ago)

Sure I do it all the time to clean stuff up. Think about it - if pan mycelium will grow on GWA or MYA, why wouldn't it grow on a BRF cake?

What is your source? Spore syringe? How old is it?


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: FunnyFungiName] * 1
    #28133862 - 01/10/23 02:57 PM (1 year, 19 days ago)

I think What Adas mean is Ambrose's cultur of bvi.

Tempting..


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InvisibleGuerrilla
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #28133866 - 01/10/23 03:01 PM (1 year, 19 days ago)

Quote:

THE MYCOSMITH said:
I think What Adas mean is Ambrose's cultur of bvi.

Tempting..






You don't say?

Sorry, sorry. :rofl: Couldn't help myself.


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Guerrilla] * 3
    #28133885 - 01/10/23 03:18 PM (1 year, 19 days ago)

No. I mean Ambrose's culture of Subtrops.


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InvisibleGuerrilla
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 2
    #28133904 - 01/10/23 03:31 PM (1 year, 19 days ago)

I missed that. I knew what I meant :rofl:

Adas I'm literally just about to streak the CPX and Bispo you gifted me.

Hopefully I didn't leave it too long.


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Offlineodissey
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Re: Official Copelandia/Panaeolus Thread [Re: Guerrilla] * 2
    #28133910 - 01/10/23 03:36 PM (1 year, 19 days ago)

Quote:

Guerrilla said:
I missed that. I knew what I meant :rofl:

Adas I'm literally just about to streak the CPX and Bispo you gifted me.

Hopefully I didn't leave it too long.



Just did that a few hours ago as well haha both cpx and bisporus from Adas.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28133913 - 01/10/23 03:37 PM (1 year, 19 days ago)

:dancingshroom: Next year I'd like to try suptrops :naughty:


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Offlineodissey
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #28133923 - 01/10/23 03:44 PM (1 year, 19 days ago)

Quote:

Baba Yaga said:
:dancingshroom: Next year I'd like to try suptrops :naughty:



Do you have a print? I still have a few reserved. Never returned your awesome pan cambo offer. Still need to revisit those, they were super fast but contamed on me and then life hit me :shrug:


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: odissey] * 2
    #28133960 - 01/10/23 04:08 PM (1 year, 19 days ago)

Thanks, I might hit you up on this later this year.


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OfflineFunnyFungiName
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #28134004 - 01/10/23 04:33 PM (1 year, 19 days ago)

Quote:

Hindsight said:
Sure I do it all the time to clean stuff up. Think about it - if pan mycelium will grow on GWA or MYA, why wouldn't it grow on a BRF cake?

What is your source? Spore syringe? How old is it?




Im using a print that is over a year old. Ive only tried lme agar so far.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: FunnyFungiName] * 2
    #28134201 - 01/10/23 06:32 PM (1 year, 19 days ago)

Oh gotcha sorry I re-read your post and between your first and second post, I am now understanding that you just can't get the pans to grow from spore - it's not that you are getting tons of bacteria instead - it's just that the spores won't germinate right? If they won't germ on agar, I don't think there is any better chance of them germing on a BRF cake. We usually use cakes on bacterial spore syringes as a way to be able to transfer away from the bacteria.

If my understanding of your situation is correct - that you aren't getting bacteria on your plates - you just aren't getting germination, you have a few options:
1. Make softer agar. Use 16g of agar per 1000ml water instead of the usual 20. You can add peptone too - some feel it helps but I'm on the fence.
2. Mame a spore syringe and let the spores sit in it for 24 hours before squirting a drop of it onto a plate
3. Use the syringe from #2 above to squirt some of the spore solution (like 1ml of it) onto a BRF cake

If none of the above works, your spores may simply no longer be viable. That's not uncommon. Different spores expire faster and slower than others, and some of it also has to do with where/how you stored them. If you need a pan print, I'd be happy to send you a fresh one to get you back going again.


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Offlinemushroom_gnome
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28134241 - 01/10/23 07:04 PM (1 year, 19 days ago)

Are BVI actually more potent than other pans or is that just going around because of the one fruit that tested very high in the cup not too long ago?


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OfflineFunnyFungiName
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #28134283 - 01/10/23 07:24 PM (1 year, 19 days ago)

Thank you Hindsight, much appreciated!

I have a couple different prints to try now, although I think the issue is most likely the thickness of my agar.


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Invisiblecooleko
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Re: Official Copelandia/Panaeolus Thread [Re: mushroom_gnome]
    #28134379 - 01/10/23 08:14 PM (1 year, 18 days ago)

Quote:

mushroom_gnome said:
Are BVI actually more potent than other pans or is that just going around because of the one fruit that tested very high in the cup not too long ago?




If you filter google searches to only articles before the date that was released you will see that no one ever implies BVIs are the most potent of the pan cyans before then. So I think it's because of the cup.

I think BVIs are the easiest pan cyans to get. Seems everyone has them right now.


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InvisibleImatryin
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox] * 2
    #28134434 - 01/10/23 08:53 PM (1 year, 18 days ago)

Is there hope for this BVI clone attempt?  I pulled these from stipes.  Nine additional plates were  bacterial, moldy or both.





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