Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: < First | < Back | 1394 | 1395 | 1396 | 1397 | 1398 | 1399 | 1400 | 1401 | 1402 | 1403 | 1404 | 1405 | 1406 | 1407 | 1408 | 1409 | 1410 | 1411 | 1412 | 1413 | 1414 | Next > | Last >
Invisibleruawakeyet
 User Gallery

Registered: 05/03/21
Posts: 1,862
Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28123750 - 01/04/23 12:19 AM (1 year, 25 days ago)

Quote:

Shrimps said:
Edit:
But QQ, Doctors use ships on bottles all the time when I get some Inoculations. Why arn't they such concered about steril technique there? Does the body fight off those "contams"?




That's a great question, one that I have thought about before.

A) Those inoculations contain ingredients that help to kill any contaminants that are introduced.

B) Just like Rusty said, our bodies have an immune system that can usually deal with a minute amount of pathogens, where as a jar of LC or grain has no immune system at all. 100% colonized grain is provided an immune system of sorts thanks to the mycelium, and bulk substrate has an immune system of sorts thanks to the friendly bacteria leftover after pasteurization.

I find it amusing that amateur mycologists have to be MORE sterile than medical workers, with the exception of surgeons performing deep surgeries, like bone or organ, etc.


Extras: Filter Print Post Top
InvisibleShrimps
Traveler
I'm a teapot


Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 3
    #28123751 - 01/04/23 12:22 AM (1 year, 25 days ago)

Yeah well, they just sometimes forget siccors or clamps in the body :lol:


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Edited by Shrimps (01/04/23 12:22 AM)


Extras: Filter Print Post Top
Invisiblecambocyan

Registered: 06/24/20
Posts: 74
Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 10
    #28123965 - 01/04/23 07:54 AM (1 year, 25 days ago)

Copelandia Cambodginiensis FSE



--------------------


Extras: Filter Print Post Top
OnlineYoshiTrainer
Onion tied to belt
 User Gallery


Registered: 04/30/22
Posts: 1,208
Loc: Castles made of sand
Last seen: 58 seconds
Re: Official Copelandia/Panaeolus Thread [Re: cambocyan] * 1
    #28124058 - 01/04/23 09:22 AM (1 year, 25 days ago)

Those are beautiful Cambo! What type of sub are you using? Do you run a tent?


Extras: Filter Print Post Top
Invisiblecambocyan

Registered: 06/24/20
Posts: 74
Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] * 1
    #28124135 - 01/04/23 10:10 AM (1 year, 25 days ago)

Thanks Yoshi this substrate is wbs to pasteurized manure cased with peat/verm 50/50 this was in a jcm chamber but id perfer a martha as others have mentioned


--------------------


Extras: Filter Print Post Top
InvisibleShrimps
Traveler
I'm a teapot


Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
Re: Official Copelandia/Panaeolus Thread [Re: cambocyan]
    #28124140 - 01/04/23 10:17 AM (1 year, 25 days ago)

For what's the FSE in "Copelandia Cambodginiensis FSE".

Is it something FSE "made"?


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Extras: Filter Print Post Top
Offlinedonrjuan
On the Grind


Registered: 10/20/10
Posts: 534
Loc: State of Boredom
Last seen: 4 hours, 56 minutes
Re: Official Copelandia/Panaeolus Thread [Re: cambocyan]
    #28124146 - 01/04/23 10:19 AM (1 year, 25 days ago)

Hey all, QQ. Can this species be stored on slants in the fridge like most others or will it kill them? I remember something about that from years ago and haven't found the answer in the search.

In the middle of transferring several plates of them now.


Extras: Filter Print Post Top
Invisiblecambocyan

Registered: 06/24/20
Posts: 74
Re: Official Copelandia/Panaeolus Thread [Re: donrjuan] * 2
    #28124166 - 01/04/23 10:35 AM (1 year, 25 days ago)

FSE is where it was originally donated
it states in the description
-origen ThailandΓ©s another uncommon cambodginiensis variety.


--------------------


Extras: Filter Print Post Top
OfflineHindsight
Mad Scientist
 User Gallery

Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: donrjuan] * 1
    #28124207 - 01/04/23 11:20 AM (1 year, 25 days ago)

Quote:

donrjuan said:
Hey all, QQ. Can this species be stored on slants in the fridge like most others or will it kill them? I remember something about that from years ago and haven't found the answer in the search.

In the middle of transferring several plates of them now.




Some have had good success with that. I think Adas even did a thing where he froze pan mycelium IIRC but it was like a year ago so my memory is fading. I personally haven't had a ton of luck with pan slants in the fridge, but I haven't tried too much. These days I just do long-term storage via room temp sterile distilled water vials. Just take a small piece of colonized agar and drop it into a sterile vial of water, then store it in a dark place. It has worked well for me. Recently revived my 12-month old BVI clone culture from one of those vials. Just took a drop of it out with a syringe and injected that into a fresh jar of LC. 7 days later, I had LC ready to go to grain.


Extras: Filter Print Post Top
OfflineBlueAndOrange
Psychedelic success story…
Male User Gallery


Registered: 01/11/22
Posts: 1,076
Last seen: 17 hours, 42 minutes
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 2
    #28124242 - 01/04/23 11:50 AM (1 year, 25 days ago)

Quote:

Hindsight said:
Quote:

donrjuan said:
Hey all, QQ. Can this species be stored on slants in the fridge like most others or will it kill them? I remember something about that from years ago and haven't found the answer in the search.

In the middle of transferring several plates of them now.




Some have had good success with that. I think Adas even did a thing where he froze pan mycelium IIRC but it was like a year ago so my memory is fading. I personally haven't had a ton of luck with pan slants in the fridge, but I haven't tried too much. These days I just do long-term storage via room temp sterile distilled water vials. Just take a small piece of colonized agar and drop it into a sterile vial of water, then store it in a dark place. It has worked well for me. Recently revived my 12-month old BVI clone culture from one of those vials. Just took a drop of it out with a syringe and injected that into a fresh jar of LC. 7 days later, I had LC ready to go to grain.




Hindsight, I like the way you work and the way you write about it. I’m basing my pan experiments based on your methods. I’m also loving the idea of moving into LC as well this year.

Do you do SSLC ever?


Extras: Filter Print Post Top
OfflineHindsight
Mad Scientist
 User Gallery

Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange] * 2
    #28124306 - 01/04/23 12:37 PM (1 year, 25 days ago)

Thanks for the kind words.

Yes actually, I started using SSLC a few months ago and really like it but am now going back to regular LC for a while. The problem with SSLC is that it is cloudy so you can't easily tell if the SSLC is contaminated. I have had instances where I didn't detect some incredibly sneaky bacteria on a plate, but it clouded up my regular LC when I put a transfer from the plate to the LC. I like this aspect of traditional LC, as another tool to ensuring my culture is perfectly clean.

SSLC does seem to colonize a bit faster than regular LC but I have played around with regular LC nutrient strengths and found that 0.8% LME produces fast growth and complete colonization, so that would be 4grams of LME for 500ml of water.


Extras: Filter Print Post Top
InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange] * 5
    #28124342 - 01/04/23 01:05 PM (1 year, 25 days ago)

Not trying to prove anything with this but I thought it is something interesting about bad looking spawn.

The jar below is a MS Jambo culture on whole barley which I was trying out as potential new grain but would consistently turn out like this. I had a few of those and thought I give one a try. Gave it a good shake and split half the content of the jar between those 2 liter bottles in the last photo below. Spawned to H-manure coir substrate closer to a 1:1 ratio IIRC.






After full colonization  and consolidation it looked like this. Wasn't too concerned about the yellow cause I get this quite a bit when consolidating cambodginiensis varieties, have to admit though that the yellowing in the right photo is one of the stronger reactions I have seen so far.




After casing I had put the lids on askew. Happy to say that both bottles have fruited. Photos below were taken 16 days after spawn. The bottle that is doing better is actually the one with the stronger yellowing.




Not the best looking spawn and not the best containers to fruit but still got some fruits. Not many though I admit.
Will keep those around till they tam out.


Edited by Baba Yaga (01/04/23 01:15 PM)


Extras: Filter Print Post Top
OfflineAdas
Lonely Dreamer
Male User Gallery


Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 37 minutes, 24 seconds
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 2
    #28124377 - 01/04/23 01:50 PM (1 year, 25 days ago)

Quote:

Hindsight said:
Quote:

donrjuan said:
Hey all, QQ. Can this species be stored on slants in the fridge like most others or will it kill them? I remember something about that from years ago and haven't found the answer in the search.

In the middle of transferring several plates of them now.




Some have had good success with that. I think Adas even did a thing where he froze pan mycelium IIRC but it was like a year ago so my memory is fading. I personally haven't had a ton of luck with pan slants in the fridge, but I haven't tried too much. These days I just do long-term storage via room temp sterile distilled water vials. Just take a small piece of colonized agar and drop it into a sterile vial of water, then store it in a dark place. It has worked well for me. Recently revived my 12-month old BVI clone culture from one of those vials. Just took a drop of it out with a syringe and injected that into a fresh jar of LC. 7 days later, I had LC ready to go to grain.




Yes, and I have that documented in my journal. At first the Bunnell grew like nothing happened after spending 2 months of winter outside, but after just 1 transfer stalled and basically "died". The Tamps, however, are still growing like nothing ever happened (and I'm about to spawn them, almost a year later).

Bisporus is a different animal and it survived a winter with lowest temp of -15C IN VIVO. Not on an agar plate - in a flowerpot. Bisporus is a temperate to subtropical Pan.


Extras: Filter Print Post Top
OfflineKingCloyster
Forest Mushroom πŸ„
Male User Gallery


Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
Last seen: 1 year, 18 days
Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #28124652 - 01/04/23 05:12 PM (1 year, 24 days ago)

Do you guys soak the casing between flushes with pan cyan or do you just leave it if the casing looks moist? How many flushes can I expect?


Extras: Filter Print Post Top
Offlinemushroom_gnome
Stranger
 User Gallery

Registered: 06/30/22
Posts: 82
Last seen: 4 hours, 24 minutes
Re: Official Copelandia/Panaeolus Thread [Re: KingCloyster] * 3
    #28124661 - 01/04/23 05:23 PM (1 year, 24 days ago)

Still no idea what these things are...
I guess I should stop posting about them here since I'm pretty positive they ain't Pans.





--------------------
Mystery Mushroom


Extras: Filter Print Post Top
InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: KingCloyster] * 1
    #28124796 - 01/04/23 06:23 PM (1 year, 24 days ago)

Quote:

KingCloyster said:
Do you guys soak the casing between flushes with pan cyan or do you just leave it if the casing looks moist? How many flushes can I expect?





Since the casing and sub is kept at a relatively high moisture level by misting or other methods like a fogger throughout the grow it is not necessary to soak or do anything extra. I just keep misting as needed. Flushes are less defined IME, fruits are popping up constantly.


Extras: Filter Print Post Top
OfflineRatatoskr
Squirrel


Registered: 07/12/13
Posts: 230
Loc: Roots of Yggdrasill
Last seen: 7 days, 10 hours
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 3
    #28124838 - 01/04/23 06:43 PM (1 year, 24 days ago)

my biggest issue is cutting the ones down that still need more time while cutting the rest. kinda a constant flush...


--------------------
All is one, one is all, everything is.


Extras: Filter Print Post Top
InvisibleBaba Yaga
β™₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Ratatoskr] * 1
    #28125088 - 01/04/23 09:57 PM (1 year, 24 days ago)

When I harvest I do cut as much of what is there as I can without spending too much time.


Extras: Filter Print Post Top
InvisibleShrimps
Traveler
I'm a teapot


Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28125313 - 01/05/23 05:48 AM (1 year, 24 days ago)

Is Bisporus a slow colonizer on grain? It seems to me mine is quite slow ^^

Sadly my Pan.camb. Goliath kinda didn't recover after the last shake :undecided: going to wait two more days to see if something happens. But my Bispo is not yet shaken and looking good so far. And happy about my Nec-D, the two Jars seem almost fully colonized. Going to spawn them into tray's in a watertub. Just to save space for other grows and keep the loss small, in case they fail xD


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Edited by Shrimps (01/05/23 05:50 AM)


Extras: Filter Print Post Top
OfflineHindsight
Mad Scientist
 User Gallery

Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 3
    #28125367 - 01/05/23 07:28 AM (1 year, 24 days ago)

Bispo has been a small amount slower than average for me, but still within a fairly normal range. Sometimes instead of 2 weeks to fully colonize from an agar wedge, it might take 3.


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 1394 | 1395 | 1396 | 1397 | 1398 | 1399 | 1400 | 1401 | 1402 | 1403 | 1404 | 1405 | 1406 | 1407 | 1408 | 1409 | 1410 | 1411 | 1412 | 1413 | 1414 | Next > | Last >

Shop: Mushroom-Hut Liquid Cultures   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* The official Psilocybe natalensis thread
( 1 2 3 4 ... 515 516 )
Hillbillyjohn 328,215 10,313 01/29/24 04:10 PM
by Smellit
* Official Misc EXOTIC Thread
( 1 2 3 4 ... 132 133 )
Jakeoncid419 110,077 2,649 01/29/24 03:16 PM
by SirPsycho
* Rye-Z-Morph Bag for Panaeolus (Copelandia) Cyanescens? rob_531 4,313 12 01/10/05 12:33 PM
by agar
* Copelandia "Pan" Cyanescens sancho 4,962 6 09/07/07 10:00 AM
by suave
* Re: look at these copelandia cyanescens they are beautiful Anonymous 5,161 2 12/25/99 07:16 AM
by sean123
* which tek for copelandia cyanescens (spore print)? FfF 7,484 10 08/26/04 05:44 AM
by hawksapprentice
* Hawaiian Copelandia Grow Kit - how much more difficult are they? GabrielKnight 8,125 5 01/03/05 04:59 PM
by ticktock
* Preparing jars for Copelandia? PawPaw 1,195 14 04/02/05 01:11 PM
by PawPaw

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
924,103 topic views. 28 members, 220 guests and 48 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.045 seconds spending 0.021 seconds on 15 queries.