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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] 1
#28123750 - 01/04/23 12:19 AM (1 year, 25 days ago) |
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Quote:
Shrimps said: Edit: But QQ, Doctors use ships on bottles all the time when I get some Inoculations. Why arn't they such concered about steril technique there? Does the body fight off those "contams"?
That's a great question, one that I have thought about before.
A) Those inoculations contain ingredients that help to kill any contaminants that are introduced.
B) Just like Rusty said, our bodies have an immune system that can usually deal with a minute amount of pathogens, where as a jar of LC or grain has no immune system at all. 100% colonized grain is provided an immune system of sorts thanks to the mycelium, and bulk substrate has an immune system of sorts thanks to the friendly bacteria leftover after pasteurization.
I find it amusing that amateur mycologists have to be MORE sterile than medical workers, with the exception of surgeons performing deep surgeries, like bone or organ, etc.
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Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 3
#28123751 - 01/04/23 12:22 AM (1 year, 25 days ago) |
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Yeah well, they just sometimes forget siccors or clamps in the body
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
Edited by Shrimps (01/04/23 12:22 AM)
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cambocyan

Registered: 06/24/20
Posts: 74
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] 10
#28123965 - 01/04/23 07:54 AM (1 year, 25 days ago) |
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Copelandia Cambodginiensis FSE
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,208
Loc: Castles made of sand
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Re: Official Copelandia/Panaeolus Thread [Re: cambocyan] 1
#28124058 - 01/04/23 09:22 AM (1 year, 25 days ago) |
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Those are beautiful Cambo! What type of sub are you using? Do you run a tent?
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cambocyan

Registered: 06/24/20
Posts: 74
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 1
#28124135 - 01/04/23 10:10 AM (1 year, 25 days ago) |
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Thanks Yoshi this substrate is wbs to pasteurized manure cased with peat/verm 50/50 this was in a jcm chamber but id perfer a martha as others have mentioned
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Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
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Re: Official Copelandia/Panaeolus Thread [Re: cambocyan]
#28124140 - 01/04/23 10:17 AM (1 year, 25 days ago) |
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For what's the FSE in "Copelandia Cambodginiensis FSE".
Is it something FSE "made"?
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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donrjuan
On the Grind


Registered: 10/20/10
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Re: Official Copelandia/Panaeolus Thread [Re: cambocyan]
#28124146 - 01/04/23 10:19 AM (1 year, 25 days ago) |
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Hey all, QQ. Can this species be stored on slants in the fridge like most others or will it kill them? I remember something about that from years ago and haven't found the answer in the search.
In the middle of transferring several plates of them now.
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cambocyan

Registered: 06/24/20
Posts: 74
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Re: Official Copelandia/Panaeolus Thread [Re: donrjuan] 2
#28124166 - 01/04/23 10:35 AM (1 year, 25 days ago) |
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FSE is where it was originally donated it states in the description -origen ThailandΓ©s another uncommon cambodginiensis variety.
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
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Re: Official Copelandia/Panaeolus Thread [Re: donrjuan] 1
#28124207 - 01/04/23 11:20 AM (1 year, 25 days ago) |
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Quote:
donrjuan said: Hey all, QQ. Can this species be stored on slants in the fridge like most others or will it kill them? I remember something about that from years ago and haven't found the answer in the search.
In the middle of transferring several plates of them now.
Some have had good success with that. I think Adas even did a thing where he froze pan mycelium IIRC but it was like a year ago so my memory is fading. I personally haven't had a ton of luck with pan slants in the fridge, but I haven't tried too much. These days I just do long-term storage via room temp sterile distilled water vials. Just take a small piece of colonized agar and drop it into a sterile vial of water, then store it in a dark place. It has worked well for me. Recently revived my 12-month old BVI clone culture from one of those vials. Just took a drop of it out with a syringe and injected that into a fresh jar of LC. 7 days later, I had LC ready to go to grain.
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BlueAndOrange
Psychedelic success storyβ¦



Registered: 01/11/22
Posts: 1,076
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 2
#28124242 - 01/04/23 11:50 AM (1 year, 25 days ago) |
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Quote:
Hindsight said:
Quote:
donrjuan said: Hey all, QQ. Can this species be stored on slants in the fridge like most others or will it kill them? I remember something about that from years ago and haven't found the answer in the search.
In the middle of transferring several plates of them now.
Some have had good success with that. I think Adas even did a thing where he froze pan mycelium IIRC but it was like a year ago so my memory is fading. I personally haven't had a ton of luck with pan slants in the fridge, but I haven't tried too much. These days I just do long-term storage via room temp sterile distilled water vials. Just take a small piece of colonized agar and drop it into a sterile vial of water, then store it in a dark place. It has worked well for me. Recently revived my 12-month old BVI clone culture from one of those vials. Just took a drop of it out with a syringe and injected that into a fresh jar of LC. 7 days later, I had LC ready to go to grain.
Hindsight, I like the way you work and the way you write about it. Iβm basing my pan experiments based on your methods. Iβm also loving the idea of moving into LC as well this year.
Do you do SSLC ever?
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange] 2
#28124306 - 01/04/23 12:37 PM (1 year, 25 days ago) |
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Thanks for the kind words.
Yes actually, I started using SSLC a few months ago and really like it but am now going back to regular LC for a while. The problem with SSLC is that it is cloudy so you can't easily tell if the SSLC is contaminated. I have had instances where I didn't detect some incredibly sneaky bacteria on a plate, but it clouded up my regular LC when I put a transfer from the plate to the LC. I like this aspect of traditional LC, as another tool to ensuring my culture is perfectly clean.
SSLC does seem to colonize a bit faster than regular LC but I have played around with regular LC nutrient strengths and found that 0.8% LME produces fast growth and complete colonization, so that would be 4grams of LME for 500ml of water.
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Baba Yaga
β₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange] 5
#28124342 - 01/04/23 01:05 PM (1 year, 25 days ago) |
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Not trying to prove anything with this but I thought it is something interesting about bad looking spawn.
The jar below is a MS Jambo culture on whole barley which I was trying out as potential new grain but would consistently turn out like this. I had a few of those and thought I give one a try. Gave it a good shake and split half the content of the jar between those 2 liter bottles in the last photo below. Spawned to H-manure coir substrate closer to a 1:1 ratio IIRC.


After full colonization and consolidation it looked like this. Wasn't too concerned about the yellow cause I get this quite a bit when consolidating cambodginiensis varieties, have to admit though that the yellowing in the right photo is one of the stronger reactions I have seen so far.

After casing I had put the lids on askew. Happy to say that both bottles have fruited. Photos below were taken 16 days after spawn. The bottle that is doing better is actually the one with the stronger yellowing.

Not the best looking spawn and not the best containers to fruit but still got some fruits. Not many though I admit. Will keep those around till they tam out.
Edited by Baba Yaga (01/04/23 01:15 PM)
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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 2
#28124377 - 01/04/23 01:50 PM (1 year, 25 days ago) |
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Quote:
Hindsight said:
Quote:
donrjuan said: Hey all, QQ. Can this species be stored on slants in the fridge like most others or will it kill them? I remember something about that from years ago and haven't found the answer in the search.
In the middle of transferring several plates of them now.
Some have had good success with that. I think Adas even did a thing where he froze pan mycelium IIRC but it was like a year ago so my memory is fading. I personally haven't had a ton of luck with pan slants in the fridge, but I haven't tried too much. These days I just do long-term storage via room temp sterile distilled water vials. Just take a small piece of colonized agar and drop it into a sterile vial of water, then store it in a dark place. It has worked well for me. Recently revived my 12-month old BVI clone culture from one of those vials. Just took a drop of it out with a syringe and injected that into a fresh jar of LC. 7 days later, I had LC ready to go to grain.
Yes, and I have that documented in my journal. At first the Bunnell grew like nothing happened after spending 2 months of winter outside, but after just 1 transfer stalled and basically "died". The Tamps, however, are still growing like nothing ever happened (and I'm about to spawn them, almost a year later).
Bisporus is a different animal and it survived a winter with lowest temp of -15C IN VIVO. Not on an agar plate - in a flowerpot. Bisporus is a temperate to subtropical Pan.
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KingCloyster
Forest Mushroom π



Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#28124652 - 01/04/23 05:12 PM (1 year, 24 days ago) |
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Do you guys soak the casing between flushes with pan cyan or do you just leave it if the casing looks moist? How many flushes can I expect?
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mushroom_gnome
Stranger


Registered: 06/30/22
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Re: Official Copelandia/Panaeolus Thread [Re: KingCloyster] 3
#28124661 - 01/04/23 05:23 PM (1 year, 24 days ago) |
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Still no idea what these things are... I guess I should stop posting about them here since I'm pretty positive they ain't Pans.

-------------------- Mystery Mushroom
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Baba Yaga
β₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: KingCloyster] 1
#28124796 - 01/04/23 06:23 PM (1 year, 24 days ago) |
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Quote:
KingCloyster said: Do you guys soak the casing between flushes with pan cyan or do you just leave it if the casing looks moist? How many flushes can I expect?
Since the casing and sub is kept at a relatively high moisture level by misting or other methods like a fogger throughout the grow it is not necessary to soak or do anything extra. I just keep misting as needed. Flushes are less defined IME, fruits are popping up constantly.
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Ratatoskr
Squirrel


Registered: 07/12/13
Posts: 230
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 3
#28124838 - 01/04/23 06:43 PM (1 year, 24 days ago) |
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my biggest issue is cutting the ones down that still need more time while cutting the rest. kinda a constant flush...
-------------------- All is one, one is all, everything is.
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Baba Yaga
β₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Ratatoskr] 1
#28125088 - 01/04/23 09:57 PM (1 year, 24 days ago) |
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When I harvest I do cut as much of what is there as I can without spending too much time.
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Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28125313 - 01/05/23 05:48 AM (1 year, 24 days ago) |
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Is Bisporus a slow colonizer on grain? It seems to me mine is quite slow ^^
Sadly my Pan.camb. Goliath kinda didn't recover after the last shake going to wait two more days to see if something happens. But my Bispo is not yet shaken and looking good so far. And happy about my Nec-D, the two Jars seem almost fully colonized. Going to spawn them into tray's in a watertub. Just to save space for other grows and keep the loss small, in case they fail xD
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
Edited by Shrimps (01/05/23 05:50 AM)
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] 3
#28125367 - 01/05/23 07:28 AM (1 year, 24 days ago) |
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Bispo has been a small amount slower than average for me, but still within a fairly normal range. Sometimes instead of 2 weeks to fully colonize from an agar wedge, it might take 3.
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