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Offlinemushroom_gnome
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #28122607 - 01/03/23 12:38 PM (1 year, 26 days ago)

also, they are trying to pin HARD inside the jars...





--------------------
Mystery Mushroom


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OfflineBlueAndOrange
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28122633 - 01/03/23 01:02 PM (1 year, 26 days ago)

Quote:

Baba Yaga said:
Nice, looking forward to hear about your findings Hindsight. I think I heard this being mentioned not too long ago. Have you discussed this recently in here or in the Nat thread?


Quote:

BlueAndOrange said:
From another thread:

Quote:

jcm4620 said:
cambos and cyans grow exactly the same and potency is the same accross the species blm if u ask me. im my oponion genetics play the biggest role in potency cuz ive had cambos that r stronger than cyans at times and other times ive had cyans more potent than cambos. genetics,environment,fruit size and really everything else that contributes to the overall health of the fruits is what determines potency. most of the time the differences can only be seen on a macro and microscopic level. however some of us can indeed tell the difference at times and at other times you cant. just pick a cyan or a cambo and start from there but make sure u get a print and not a syringe.




So why is a print recommended over syringe? Is this a pan specific recommendation? I have had very good results going from syringe to agar. Much more success than working with prints. But that has been cubes and nats. I haven’t tried pans yet.





There are a lot of posts in this thread where people have had problems to get pan spores to germinate on agar and the majority were using syringes.  It doesn't sound logical but the number of cases is quite high. I think that germinating on PF-Tek pucks might mitigate the issue.




Interesting. I ordered some spore syringes and plan to germinate on agar. If they don’t germinate, I guess I’ll be less surprised. Maybe I’ll need to germinate on PF pucks.

I’ve never done PF TEK. If I germinate on a PF puck can I then take that mycelium to agar, grow it out and then go to grain?

Also, is G2G viable with the pans? Once I get some good clones going, I’ll want to multiply them and get a small stockpile of dried fruits.


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Invisiblecooleko
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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange]
    #28122651 - 01/03/23 01:20 PM (1 year, 26 days ago)

If you germinate the PF puck (I prefer EF TEK). It's probably better to wait for a fruit and clone it, but you can transfer mycelium.

I don't know of any mushroom cultures where G2G isn't viable, but since I work with LC I never G2G.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange]
    #28122658 - 01/03/23 01:22 PM (1 year, 26 days ago)

Quote:

BlueAndOrange said:
I’ve never done PF TEK. If I germinate on a PF puck can I then take that mycelium to agar, grow it out and then go to grain?




Yes I do it all the time. Just grab some of the mycelium that is growing up into the air off the cake and put that on a plate.

Quote:

BlueAndOrange said:
Also, is G2G viable with the pans? Once I get some good clones going, I’ll want to multiply them and get a small stockpile of dried fruits.





Yes, no different than any other varieties in this regard. G2G has added risk of contamination though. I prefer LC. You can go from LC to having however many fully colonized jars you want in just 8 days. 100 jars? 8 days.


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: mushroom_gnome]
    #28122685 - 01/03/23 01:46 PM (1 year, 26 days ago)

Quote:

mushroom_gnome said:
also, they are trying to pin HARD inside the jars...








Whatever they are, they look great. Please recover the culture and document the type of growth on agar, grain etc. It may turn out to be a rare edible or something. It kinda reminds me of Hypsizygus a bit.


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Offlinemushroom_gnome
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28122708 - 01/03/23 02:01 PM (1 year, 26 days ago)

Quote:

Adas said:
Quote:

mushroom_gnome said:
also, they are trying to pin HARD inside the jars...








Whatever they are, they look great. Please recover the culture and document the type of growth on agar, grain etc. It may turn out to be a rare edible or something. It kinda reminds me of Hypsizygus a bit.





I'll get the culture back on agar. Here is a photo of the culture before I dumped it. The spore print that this came from is black and labeled as Cambo Jamaica



--------------------
Mystery Mushroom


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: mushroom_gnome]
    #28122827 - 01/03/23 02:54 PM (1 year, 25 days ago)

Quote:

mushroom_gnome said:
also, they are trying to pin HARD inside the jars...










Those caps should have come out in some shade of tan by now and the shape is not right either. Extract a few of those blobs and or fruits and cut them open. Never seen a pan not bruise dark blue so this should solve the question if they are pans or not or not or whether it is an active species at all. Or can you see any blue at all?

Could be a vendor mislabel.


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Offlinemushroom_gnome
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28122864 - 01/03/23 03:08 PM (1 year, 25 days ago)

Quote:

Baba Yaga said:
Quote:

mushroom_gnome said:
also, they are trying to pin HARD inside the jars...








Those caps should have come out in some shade of tan by now and the shape is not right either. Extract a few of those blobs and or fruits and cut them open. Never seen a pan not bruise dark blue so this should solve the question if they are pans or not or not or whether it is an active species at all. Or can you see any blue at all?

Could be a vendor mislabel.




Good idea. No bluing, hollow stem. That pretty much confirms: not a pan. Now the questions. What the fuck is it?






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Mystery Mushroom


Edited by mushroom_gnome (01/03/23 03:09 PM)


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: mushroom_gnome] * 1
    #28122881 - 01/03/23 03:15 PM (1 year, 25 days ago)

Quote:

mushroom_gnome said:

Good idea. No bluing, hollow stem. That pretty much confirms: not a pan. Now the questions. What the fuck is it?









Ok, that is a definite no for pan and probably active as well and yes, look at that hollow stem.

Probably too early to take it to the Hunting & ID forums, keep documenting though and take it there eventually if no one can crack that mystery over here.


Send me your addy and I'll send you a Jambo print.


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OfflineBlueAndOrange
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28123374 - 01/03/23 08:30 PM (1 year, 25 days ago)

Quote:

Hindsight said:
Quote:

BlueAndOrange said:
I’ve never done PF TEK. If I germinate on a PF puck can I then take that mycelium to agar, grow it out and then go to grain?




Yes I do it all the time. Just grab some of the mycelium that is growing up into the air off the cake and put that on a plate.

Quote:

BlueAndOrange said:
Also, is G2G viable with the pans? Once I get some good clones going, I’ll want to multiply them and get a small stockpile of dried fruits.





Yes, no different than any other varieties in this regard. G2G has added risk of contamination though. I prefer LC. You can go from LC to having however many fully colonized jars you want in just 8 days. 100 jars? 8 days.




Well, sounds like 2023 is the year to learn LC. I’ve done just over 100 jars g2g with 1 contam due to a malfunction lid seal. I work at a flow hood and have had good results, but it’s a lot slower than 8 days.


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OfflineExcess Taters
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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange]
    #28123603 - 01/03/23 10:25 PM (1 year, 25 days ago)

Quote:

BlueAndOrange said:
Quote:

Hindsight said:
Quote:

BlueAndOrange said:
I’ve never done PF TEK. If I germinate on a PF puck can I then take that mycelium to agar, grow it out and then go to grain?




Yes I do it all the time. Just grab some of the mycelium that is growing up into the air off the cake and put that on a plate.

Quote:

BlueAndOrange said:
Also, is G2G viable with the pans? Once I get some good clones going, I’ll want to multiply them and get a small stockpile of dried fruits.





Yes, no different than any other varieties in this regard. G2G has added risk of contamination though. I prefer LC. You can go from LC to having however many fully colonized jars you want in just 8 days. 100 jars? 8 days.




Well, sounds like 2023 is the year to learn LC. I’ve done just over 100 jars g2g with 1 contam due to a malfunction lid seal. I work at a flow hood and have had good results, but it’s a lot slower than 8 days.




Might I make a recommendation?  JOSEX POKE!

https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=24740168&PHPSESSID=&fpart=1#24740168


--------------------
If you've never grown mushrooms before, here's how you start.  First, follow the Updated PF Tek, put 4 holes in jar lids not 1, and use a water tub! 
The next move is Shoebox Tek. After that you move onto grains, agar, monotubs.  Agar is easy, just do it.
Other useful links - Picture guide for how things should look and proper surface conditions guide
Growing APE or PE?  P9 pseudo casing tek


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OfflineBlueAndOrange
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Re: Official Copelandia/Panaeolus Thread [Re: Excess Taters]
    #28123643 - 01/03/23 10:59 PM (1 year, 25 days ago)

I appreciate the guidance to the josex poke LC thread. Thanks!


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange]
    #28123669 - 01/03/23 11:19 PM (1 year, 25 days ago)

May I also add: if I ever start using LCs again, I will be adopting Fahtster's Steam Pen method to sterilize SHIPs on my LC lids.

https://www.shroomery.org/forums/showflat.php/Number/27586183


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #28123671 - 01/03/23 11:21 PM (1 year, 25 days ago)

Just curious about, would that also work just inoculating it under alcohol? Like filling a bottle with alcohol, and inoculating the whole thing submerged? My LC containers don't have a airfilter, only ship ^^(and I have never used LC, just made some for science and shits n giggles).


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28123679 - 01/03/23 11:25 PM (1 year, 25 days ago)

I would be worried about the alcohol killing my desired tissue, but I really couldn't say if it would work or not.

I should have added above that it would take a little adaptation to use the steam pen along with the Josex Poke, or else the same risk of killing the desired tissue could apply.


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #28123684 - 01/03/23 11:27 PM (1 year, 25 days ago)

Hmmm, didn't think about that... Still kinda prefer my SAB for this kind of work xD But then I just open the lid a crack. Would be nice though to be a bit more "free" in my work... and No, I don't have the cash for a FH... T.T

Edit:
But QQ, Doctors use ships on bottles all the time when I get some Inoculations. Why arn't they such concered about steril technique there? Does the body fight off those "contams"?


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Edited by Shrimps (01/03/23 11:32 PM)


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OfflineRusty2096
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 2
    #28123693 - 01/03/23 11:34 PM (1 year, 25 days ago)

I'd just be too much trouble to make every injections 100% sterile

So they live with 99.9%

Why do you think so many viruses and illnesses are transmitted inside hospitals.


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Rusty2096]
    #28123702 - 01/03/23 11:39 PM (1 year, 25 days ago)

well, tbf I never got anything from a hospital. When I get viruses or transmitted illnesses is because of crowded places like busses or trains ^^ But I get your point. Thanks Rusty :hug:


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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OfflineRusty2096
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps]
    #28123716 - 01/03/23 11:44 PM (1 year, 25 days ago)

:hug:

(you probably never got anything from the hospital because you are probably healthy and your system can fight off most nasties. People with compromised immune systems, cancer, etc. are more at risk)


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Rusty2096] * 3
    #28123720 - 01/03/23 11:46 PM (1 year, 25 days ago)

Had two time a tumor removed tbh and was like every month in the hospital as a kid. I had to climb up every where I was a very curious little baby shrimps ^^


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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