|
JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
|
Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#28117493 - 12/30/22 12:57 PM (1 year, 30 days ago) |
|
|
How do you clone mature pans? Taking tissue from the thin ass stem seems difficult. Maybe taking a pin clone is the way?
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
|
Re: Official Copelandia/Panaeolus Thread [Re: JHOVA] 1
#28117496 - 12/30/22 12:58 PM (1 year, 30 days ago) |
|
|
Quote:
JHOVA said: How do you clone mature pans? Taking tissue from the thin ass stem seems difficult. Maybe taking a pin clone is the way?
This might help: Simple Cloning TEK by Adas
Edited by Shrimps (12/30/22 01:04 PM)
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 15 minutes, 26 seconds
|
Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] 2
#28117498 - 12/30/22 01:00 PM (1 year, 30 days ago) |
|
|
Thanks for linking my tek mate
|
Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
|
Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#28117499 - 12/30/22 01:00 PM (1 year, 30 days ago) |
|
|
Just linking what you have given us <3 Thanks alot man, really appreciate all the work you've done for us!
|
JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
|
Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] 1
#28117521 - 12/30/22 01:31 PM (1 year, 30 days ago) |
|
|
Man splitting a pan stem evenly sounds hard as fuck.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
|
Re: Official Copelandia/Panaeolus Thread [Re: JHOVA]
#28117524 - 12/30/22 01:33 PM (1 year, 30 days ago) |
|
|
Or what about just poke with a syringe with some steril water inside through the stem and squirt it in a petridish? (haven't clone pans myself yet^^)
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 15 minutes, 26 seconds
|
Re: Official Copelandia/Panaeolus Thread [Re: Shrimps]
#28117535 - 12/30/22 01:42 PM (1 year, 30 days ago) |
|
|
Don't have to split it evenly. That's the good part
|
SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
|
Re: Official Copelandia/Panaeolus Thread [Re: JHOVA] 1
#28117539 - 12/30/22 01:43 PM (1 year, 30 days ago) |
|
|
Quote:
JHOVA said: How do you clone mature pans? Taking tissue from the thin ass stem seems difficult. Maybe taking a pin clone is the way?

…Also Adas’ tek!
|
mushroom_gnome
Stranger


Registered: 06/30/22
Posts: 82
Last seen: 4 hours, 2 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: JHOVA]
#28117556 - 12/30/22 01:55 PM (1 year, 30 days ago) |
|
|
Quote:
JHOVA said: How do you clone mature pans? Taking tissue from the thin ass stem seems difficult. Maybe taking a pin clone is the way?
Carefully with a sharp knife after splitting or a little wipe of alcohol on the outside of the stipe followed by a needle biopsy. Both have worked, just be vigilant with transferring away from any contams that hop on for the ride.
-------------------- Mystery Mushroom
|
FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
|
Re: Official Copelandia/Panaeolus Thread [Re: mushroom_gnome] 3
#28117574 - 12/30/22 02:12 PM (1 year, 30 days ago) |
|
|
i usually just remove the cap (because of spores) and throw the whole stem on agar make sure the stem doesn't tumble and roll around on the plate or else it will for sure contaminate. it works for me about 80-90% of the time.
-------------------- (Yes, the egg is real)
How to post pics
|
JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
|
Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg]
#28117608 - 12/30/22 02:43 PM (1 year, 30 days ago) |
|
|
Yea that sounds easier FriedEgg
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 15 minutes, 26 seconds
|
Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg] 1
#28117647 - 12/30/22 03:20 PM (1 year, 30 days ago) |
|
|
Quote:
FriedEgg said: i usually just remove the cap (because of spores) and throw the whole stem on agar make sure the stem doesn't tumble and roll around on the plate or else it will for sure contaminate. it works for me about 80-90% of the time.
That's definitely a pretty good option I feel. Often simpler is better, depending on situation.
|
elpico
Stranger

Registered: 11/28/16
Posts: 315
|
Re: Official Copelandia/Panaeolus Thread [Re: 99.99] 2
#28117811 - 12/30/22 05:46 PM (1 year, 29 days ago) |
|
|
Whatever works for you
Edited by elpico (01/01/23 04:21 PM)
|
KingCloyster
Forest Mushroom 🍄



Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
Last seen: 1 year, 18 days
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#28117984 - 12/30/22 08:22 PM (1 year, 29 days ago) |
|
|
Quote:
Hindsight said: I find caps bleaching lighter than normal is usually due to dry conditions in the tent. Your pics look dry to me - both the fruits and the casing. You mention you're having trouble keeping the casing moist after seeing pins - that confirms you aren't getting enough moisture into the tent.
I set my tent cycles almost exclusively based on casing moisture. I have a lot of info on cycles here: https://www.shroomery.org/forums/showflat.php/Number/27993602
It's very important to try to stick with the same total cycle time, and hopefully also the same vent time, and then only adjust your humidity time. It's too hard to narrow in on the exact cycles you need if you change more than one thing at a time. 85% RH for your dry rest cycle is great - that's what I get too. So you need to dump more moisture into the tent to keep the casing moisture ideal. I have found if you keep the casing moisture ideal, your fruits will also be happy. It seems like you have your intake fan and your humidifier on separate cycles/systems/timers/controllers. I am not going to say you shouldn't do that, but it is just more variables you have to juggle. The way I have it, the intake fan kicks on when the fogger runs. Fogger runs for 2 - 3 mins, then the vent fan immediately runs for 1 min, then the tent rests for like 9 or 10 mins or something like that I forget. The only thing I adjust is the duration of the fog cycle.
In your tek it was 26 minutes rest instead of 10 so I’m sticking with that. The humidity hits right around 80 once they kick on now, since I adjusted my fan setting according to your tek. I had all of my fans on high speed and switched them both to low, which seems to help maintain that humidity level much longer and hopefully stop drying out my casings.
The dual timers in sync makes way better sense than the ink bird controller to get everything synced too. Those inkbird rH controllers have been ass for me. Watched mine get stuck at 94rH on the downslope for about 30 minutes once, then started climbing once it got unstuck just to get stuck again! SMH
I do appreciate the help and knowledge hindsight. Your tek is great and so far has been the gold standard from what I’ve seen when it comes to Martha teks for pans. Very well done
Edited by KingCloyster (12/30/22 09:49 PM)
|
tregar
Stranger


Registered: 08/30/04
Posts: 1,049
Last seen: 8 hours, 31 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: KingCloyster]
#28118409 - 12/31/22 06:15 AM (1 year, 29 days ago) |
|
|
Compilation of panaeolus cyanescens or pan cyan experiences & combo with THH or tetrahydroharmine, like organic psilocin LSD, extremely visual, energizing like mescaline, music enhancing & super euphoric:
https://www.shroomery.org/forums/showflat.php/Number/28108398
|
Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
|
Re: Official Copelandia/Panaeolus Thread [Re: KingCloyster] 2
#28118427 - 12/31/22 06:41 AM (1 year, 29 days ago) |
|
|
Quote:
KingCloyster said: In your tek it was 26 minutes rest instead of 10 so I’m sticking with that. The humidity hits right around 80 once they kick on now, since I adjusted my fan setting according to your tek. I had all of my fans on high speed and switched them both to low, which seems to help maintain that humidity level much longer and hopefully stop drying out my casings.
The dual timers in sync makes way better sense than the ink bird controller to get everything synced too. Those inkbird rH controllers have been ass for me. Watched mine get stuck at 94rH on the downslope for about 30 minutes once, then started climbing once it got unstuck just to get stuck again! SMH
I do appreciate the help and knowledge hindsight. Your tek is great and so far has been the gold standard from what I’ve seen when it comes to Martha teks for pans. Very well done
Thanks for the kind words. Yeah I do notice my write-up shows 26 mins off. I actually started out with about half that, and grew that way for over a year, then I decided to try longer fog and longer rest cycles just to see if the fruits liked it better. It worked ok but I wasn't as happy with it so I recently went back to the shorter cycles which are what I run now. I'll update my write-up. I keep a piece of paper tacked to the wall next to the tent and I write down my cycle times there. Every time I need to make an adjustment (too wet or too dry), I write it down on a new line on that paper. This keeps it easy to track and easy to go back. I just looked at my chart and right now I'm running 3m on, 1 vent, 10 rest. So long as heat and humidity in the grow room stays constant (via my heater on an inkbird and my room humidifier on a built-in humidistat), I rarely need to change it and if I do, it's never more than adjusting the on-cycle time up or down 15, maybe 30 seconds max. From memory, I am generally running at 2:30 fog cycle. But there are too man variables for you to run exactly the same - however, I do suggest going down to a shorter cycle at some point since that is what I have had the most success with.
Yeah screw those Inkbird humidity controllers. They are handy for a reference point to check things occasionally (like to see how dry your tent gets at the very end of the rest) but they do get stuck and they do not perform well above 90% as they get water-logged. They also just don't measure change quick enough. It would be fine if you were running a huge fruiting chamber the size of a bedroom where things change very, very slowly, but definitely not for a tent. And even then they still don't like living at > 90% RH. I do use the Inkbird temp controller for my electric space heater in the grow closet though. Works great for that - but again, it's heating a large closet and not a small tent so it works well.
|
odissey
Psychonaut


Registered: 01/19/21
Posts: 487
Loc: Europe
Last seen: 13 days, 8 hours
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#28118498 - 12/31/22 08:05 AM (1 year, 29 days ago) |
|
|
Quote:
tregar said: Compilation of panaeolus cyanescens or pan cyan experiences & combo with THH or tetrahydroharmine, like organic psilocin LSD, extremely visual, energizing like mescaline, music enhancing & super euphoric:
https://www.shroomery.org/forums/showflat.php/Number/28108398
This is interesting. I want to try vaporizing/smoking pure harmalas or b.cappi leaf during a trip soon.
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 15 minutes, 26 seconds
|
Re: Official Copelandia/Panaeolus Thread [Re: odissey] 12
#28118923 - 12/31/22 02:54 PM (1 year, 29 days ago) |
|
|
Not sure if anyone's tested this combo yet, but today I did my traditional new year's fasting + hike + trip. This time 1g Tamps (CPX) and 0.5g Bisporus. Didnt feel much until it started hitting me almost an hour in. It was pretty damn potent, colors, lights, cool visuals and amazing body vibrations (from the Bisporus specifically I think). The visuals were not as cool or clear as with 1g Bisporus, but still pretty damn godly. It's one of these experiences where you have trouble recollecting it all, it was kinda chaotic but in a good way.
Lots of contemplating death and dying, life and living, what it means etc.. having recently lost a loved one and it still hurts. So lots of processing but after all this I'm a wiser and gentler being. I still have a lot of work to do on my neuroses and things like this, but I know I can improve with time.
I wish you all the very best in 2023. One day we came to this planet and one day we will leave, but let's leave a damn good trail behind us. Love y'all 
|
Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
|
Re: Official Copelandia/Panaeolus Thread [Re: Adas] 4
#28118937 - 12/31/22 03:06 PM (1 year, 29 days ago) |
|
|
Quote:
Adas said: Not sure if anyone's tested this combo yet, but today I did my traditional new year's fasting + hike + trip. This time 1g Tamps (CPX) and 0.5g Bisporus. Didnt feel much until it started hitting me almost an hour in. It was pretty damn potent, colors, lights, cool visuals and amazing body vibrations (from the Bisporus specifically I think). The visuals were not as cool or clear as with 1g Bisporus, but still pretty damn godly. It's one of these experiences where you have trouble recollecting it all, it was kinda chaotic but in a good way.
Lots of contemplating death and dying, life and living, what it means etc.. having recently lost a loved one and it still hurts. So lots of processing but after all this I'm a wiser and gentler being. I still have a lot of work to do on my neuroses and things like this, but I know I can improve with time.
I wish you all the very best in 2023. One day we came to this planet and one day we will leave, but let's leave a damn good trail behind us. Love y'all  
Thanks for sharing man! Glad you had a great time with working through all that! I can kinda feel with you, since I also have lost loved ones and still in recovery from the trauma. Haven't tript yet, going to wait until I am off my meds, but hearing you write about your trip and your recent loss (which hurts me deeply and I wish you alot of energy!) gives me strength that I can have similar experiences in the futur!
I wish you and all the others aswell all the best, a huge pile of luck and all the love in the world for your upcoming year 2023!
Mush love your friendly crustacean
Shrimps
Edit: Forgot to you all!
Edited by Shrimps (12/31/22 03:08 PM)
|
Scotay
The Mad Mush Guy

Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 14 hours
|
Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] 1
#28119290 - 12/31/22 08:12 PM (1 year, 28 days ago) |
|
|
Happy new year all you filthy panheads out there!!! Everyone have a blast a be safe! Hopefully next year is filled with mushiness for all of y'all!!! Thanks for answering my silly newbie questions and trying to help an old dog learn new tricks!! I appreciate your kindness from the bottom of my mycelium!!! 😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
|
|