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Offlinegoatboy666
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Re: Official Copelandia/Panaeolus Thread [Re: Egon_Spengler] * 1
    #28078991 - 12/02/22 01:30 AM (1 year, 1 month ago)

I've never noticed pans or tamps pin on agar.  Maybe someone out there has had this? Im sure its happened but I've never seen it.


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: goatboy666] * 2
    #28078993 - 12/02/22 01:34 AM (1 year, 1 month ago)



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Offlinegoatboy666
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps]
    #28079062 - 12/02/22 04:07 AM (1 year, 1 month ago)

Cool, had a feeling someone would have had pan pins on agar


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Offlinegoatboy666
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Re: Official Copelandia/Panaeolus Thread [Re: goatboy666] * 3
    #28079068 - 12/02/22 04:20 AM (1 year, 1 month ago)

Wild coast



BVI



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InvisibleEgon_Spengler
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps]
    #28079099 - 12/02/22 05:33 AM (1 year, 1 month ago)

Quote:

Shrimps said:
Look here: https://www.shroomery.org/forums/showflat.php/Number/28078087#28078087



Thanks, Shrimps!


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Egon_Spengler] * 1
    #28079157 - 12/02/22 06:58 AM (1 year, 1 month ago)

I have personally never had a pan cyan pin on agar. They are more picky about fruiting conditions. It CAN happen, but I would not make plate pins my primary strategy for obtaining clones. Save your plates after spawning to a substrate and keep an eye on them in case you get lucky.


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InvisibleEgon_Spengler
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28079161 - 12/02/22 07:01 AM (1 year, 1 month ago)

Quote:

Hindsight said:
I have personally never had a pan cyan pin on agar. They are more picky about fruiting conditions. It CAN happen, but I would not make plate pins my primary strategy for obtaining clones. Save your plates after spawning to a substrate and keep an eye on them in case you get lucky.




Thanks, I suspected it must be pretty rare, since I haven’t seen many folks post agar pins here the way i seem to see it pretty frequently in the cube cult threads.

I’ll hang on to them for a while just to see what happens. If nothing pins in vitro, no biggie. I was just wondering.

Thanks again!


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Egon_Spengler]
    #28079167 - 12/02/22 07:11 AM (1 year, 1 month ago)

So just a quick question, it seems (due to an kind of sour smell) and alot of visible metabolites in the other tub, both tub's are very probably contaminated by bacteria.

Also noticed some mistakes I've made, but cased them yesterday anyway(also just to get used to the procedure). I am not really expecting any pinning, but will still give it a shot. But should I say, like if there isn't any real progress in 1 week, should I toss it and hit some new rye up with the myc I've got left on agar and start over?

Not really sure, I have a bit lower temperatures as you know (22Β°C-24Β°C), so it could maybe take a bit longer than usual to see something, but is there like a point where you guys say it's a toss and start over again?


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I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 2
    #28079192 - 12/02/22 07:34 AM (1 year, 1 month ago)

I would wait minimum of 3 weeks and preferably 4 before giving up. Pans can take a long time to pin. For example, I have some bispos in my tent that are just now pinning after 18 days from spawning. And that's with a clone culture in ideal tent conditions. That's on the longer end of average for me but bottom line is to be patient and wait 4 weeks.


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28079227 - 12/02/22 08:16 AM (1 year, 1 month ago)

Thanks for your reply! Much appreciated <3


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I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 2
    #28079251 - 12/02/22 08:36 AM (1 year, 1 month ago)

Shrimps, I would already have fresh grain jars colonizing. No sense in waiting for the current grow to fail. (I'm not saying it definitely will, but I always try to have my next grow a couple of weeks behind at most.) If one round fails, I've got the next round already started.

Admittedly, that approach can turn into a problem if you don't have any contamination. I'm going to have more colonized grain than I can even use. I'm going to have to add a JCM chamber or make another fogging table and double my operation if I don't let any grain go to waste!


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #28079299 - 12/02/22 09:15 AM (1 year, 1 month ago)

Yeah, don't have any more space up at the moment. Maybe in 3-4 Weeks I can ditch my cube-tub for a new try with the pans :smile: Had to promise my GF I wouldn't stock up so much until we've got a bigger apartment.

But I have the mycelium on Agar, so wouldn't take much to hit up a new batch at some point. Wanted to keep that myc for any maybe failed attempts so I don't have to start from spore again ^^


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I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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OfflineSirPsycho
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 7
    #28079890 - 12/02/22 04:17 PM (1 year, 1 month ago)



Cyan huasteca RDU going to straw based bottles.


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Offlinegoatboy666
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Re: Official Copelandia/Panaeolus Thread [Re: SirPsycho]
    #28080066 - 12/02/22 05:54 PM (1 year, 1 month ago)

Only just found out about rdu (my friend is sending a print) but....could you explain the huastaeca part?  So it is pan.cyan? Or something different?


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: goatboy666] * 10
    #28080967 - 12/03/22 08:37 AM (1 year, 1 month ago)

Mystery South Africa is finally pinning:





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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28080969 - 12/03/22 08:38 AM (1 year, 1 month ago)

Nice :smile: Is that a ms culture?

Edit: maybe I should post my question here^^.


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Edited by Shrimps (12/03/22 09:04 AM)


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Invisiblestagger
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 2
    #28081223 - 12/03/22 11:53 AM (1 year, 1 month ago)

a 3rd flush for this box, does anyone have a possible explanation for the lack of spore production?  I've always been able to print these, even if most of them printed very lightly, but this is new for me.


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Offline0uroboro5
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Re: Official Copelandia/Panaeolus Thread [Re: stagger] * 5
    #28084477 - 12/05/22 09:13 AM (1 year, 1 month ago)

Talking about pans pinning on agar-
i just found a 3 month old Jambo ms petridish on my shelf.
I gonna try to clone that sweet one :smile:
If those genetics make it on agar they will fruit everywhere.



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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: stagger] * 1
    #28084499 - 12/05/22 09:31 AM (1 year, 1 month ago)

Quote:

stagger said:
a 3rd flush for this box, does anyone have a possible explanation for the lack of spore production?  I've always been able to print these, even if most of them printed very lightly, but this is new for me.





Genetics are the only explanation I can think of. I've seen various pan cultures do some strange things, from mutants to sporeless fruits.


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Offline99.99
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Re: Official Copelandia/Panaeolus Thread [Re: goatboy666] * 4
    #28084622 - 12/05/22 11:16 AM (1 year, 1 month ago)

Quote:

goatboy666 said:
I've never noticed pans or tamps pin on agar.  Maybe someone out there has had this? Im sure its happened but I've never seen it.




Yes i did and boy did they grow like crazy






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