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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#28076045 - 11/30/22 12:21 PM (1 year, 1 month ago) |
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Quote:
Mycolorado said: What’s the consensus on millet or rye/wheat/oats for pans?
I suppose what ever works best for you is the grain of choice. Don't think that pans do care much.
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado] 3
#28076050 - 11/30/22 12:24 PM (1 year, 1 month ago) |
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Quote:
Mycolorado said: What’s the consensus on millet or rye/wheat/oats for pans?
I have used all of them successfully for pans. Even blends - like 25% rye and 75% millet etc. I don't notice any of them perform any better or worse, but they can act a bit differently. I feel that millet colonizes substrate faster due to more inoculation points, but feel that rye is easier to evaluate cleanliness and hydration percentage. Both fruit well.
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Mycolorado
Hobbyist


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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#28076070 - 11/30/22 12:35 PM (1 year, 1 month ago) |
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Right on; thanks, all!
Edited by Mycolorado (11/30/22 12:37 PM)
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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28076105 - 11/30/22 01:06 PM (1 year, 1 month ago) |
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Quote:
kirkeng said: Could just be a string of bad luck, at least that’s what I’ve been telling myself when it happens, perseverance is your best friend! Also this may sound weird and non scientific but I’ve noticed a correlation between my success with the RDU and my relative stress, they seem more sensitive to outside factors like that. Probably the discouragement of the failure gets passed down through subconscious issues and mistakes. Have you tried going back to the generation that went well? Maybe that could definitively signal bad luck or give more insight to when the issue started presenting.
It's impossible because I havent saved any of the OG cultures and the spore prints will long be dead now.
Quote:
Baba Yaga said: Generally with other pans there are some lines of genetics that don't seem to fruit as happily as others. Not that I really took notes about this but for me it tends to be either a wave of good grows or a wave of under-performing depending on what LC was used to inoculte. This is probably just a good example how genetic selection from MS plates can play tricks on us as it is often overlooked as cause of failed grows and then something else is thought out to be the culprit.
I have to admit though that one year of failure it a long time but if they have been performing well to start with then I have a hard time to believe that all goes down after only a few generations.
Troubleshooting is a bitch in this hobby.
It could be, but it's not like I've changed my mycelium selection techniques much, if at all in terms of Pans (or maybe I have without realizing it entirely).
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Mycolorado
Hobbyist


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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 2
#28076154 - 11/30/22 01:43 PM (1 year, 1 month ago) |
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I just got germ off some prints from ‘18, so there’s always hope. I’m rehydrating some as well and will report on those.
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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado]
#28076169 - 11/30/22 01:53 PM (1 year, 1 month ago) |
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That's very interesting! But from my experience even Cube prints don't survive 2.5 years, while some people claim 10+
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Mycolorado
Hobbyist


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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#28076211 - 11/30/22 02:15 PM (1 year, 1 month ago) |
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I hear ya. I just germ’d some cube prints (TYC) that are around the same age or older whereas my RW prints won’t germ after a few months without rehydration.
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Shrimps
Traveler



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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#28076235 - 11/30/22 02:27 PM (1 year, 1 month ago) |
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Quote:
ruawakeyet said:
Quote:
Shrimps said: I repost my question again, kinda got lost 
Quote:
Shrimps said: Here a quick update on my two (Cop.cyan. "Jamaica") tubes:
11L Shoebox with my wpoo "experiment" spawned 14.11 (following babayagas Thread:

31L Monotube with normal hpoo spawned 21.11 following asuras Thread:

I think due to the low temperatures (21°C-23°C) the colonization is taking a bit longer. Also my Wpoo experiment had a bit a thick toplayer, so I scratched a bit of on the 21.11 and since then its recovering quite well. I think my Wpoo thingi was maybe a little bit too wet, what also seems to be adding to the longer colonization time 
I think I will wait a couple of days longer until I apply the casing layer, or what do you pan-veterans think about that?
Since that was like 2 days ago (and they don't look much different), I pasteurized now 50/50 peat/verm for the casinglayer. Only going to apply it on my mono, I think my Wpoo Shoebox might be bacterial, has a little bit a sour smell to it. But I will just leave that one for now and look what happens in a week or two. I think it's a loss ^^
But aside of that, just making those two made me realize how I could do it better next time Still got some myc on agar though.
The temperature range should be fine for colonization, but I did usually colonize the bulk in fruiting temps, so in the upper 70s.
It's hard to tell from photos, but the Wpoo tub looked dry to me, but like I said, I can't really tell. (I saw the walls of the tub didn't look dry, only the substrate.) The other tub looks kind of off, but all you can do is case it and see.
Thanks for your reply! Is it a big problem if the temperature during fruiting stays at around 72 - 75 fahrenheit? Or will they just be a bit slower similar to cubes?
Tbh, it's my first try with pans and I noticed alot that I would do different next time, so even if they won't fruit, I won't be that much disapointed, learnd alot. Will just hit up the next grains with that what I've got left on agar and do it better ;-)
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps]
#28076248 - 11/30/22 02:34 PM (1 year, 1 month ago) |
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72 to 75 should be ok, but I've never attempted at that range. I always kept a room a little warmer, around 77 or 78.
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Shrimps
Traveler



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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#28076261 - 11/30/22 02:39 PM (1 year, 1 month ago) |
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Yeah I would if I had a separate room to grow in ^^ My monos are standing in the livingroom xD
But hopefully we find a new apartment in spring, then I will just keep the room there at 77. But now it would be alot of energy waste and atm that is a bit of thing here where I am.
Thanks alot again for all the friendly help! I really appreciate you taking the time to answer my questions.
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
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I just realized... Pan cyans are also called blue meanies right?
Nm that I Googled it
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] 1
#28076487 - 11/30/22 04:36 PM (1 year, 1 month ago) |
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Quote:
Shrimps said:
Quote:
ruawakeyet said:
Quote:
Shrimps said: I repost my question again, kinda got lost 
Quote:
Shrimps said: Here a quick update on my two (Cop.cyan. "Jamaica") tubes:
11L Shoebox with my wpoo "experiment" spawned 14.11 (following babayagas Thread:

31L Monotube with normal hpoo spawned 21.11 following asuras Thread:

I think due to the low temperatures (21°C-23°C) the colonization is taking a bit longer. Also my Wpoo experiment had a bit a thick toplayer, so I scratched a bit of on the 21.11 and since then its recovering quite well. I think my Wpoo thingi was maybe a little bit too wet, what also seems to be adding to the longer colonization time 
I think I will wait a couple of days longer until I apply the casing layer, or what do you pan-veterans think about that?
Since that was like 2 days ago (and they don't look much different), I pasteurized now 50/50 peat/verm for the casinglayer. Only going to apply it on my mono, I think my Wpoo Shoebox might be bacterial, has a little bit a sour smell to it. But I will just leave that one for now and look what happens in a week or two. I think it's a loss ^^
But aside of that, just making those two made me realize how I could do it better next time Still got some myc on agar though.
The temperature range should be fine for colonization, but I did usually colonize the bulk in fruiting temps, so in the upper 70s.
It's hard to tell from photos, but the Wpoo tub looked dry to me, but like I said, I can't really tell. (I saw the walls of the tub didn't look dry, only the substrate.) The other tub looks kind of off, but all you can do is case it and see.
Thanks for your reply! Is it a big problem if the temperature during fruiting stays at around 72 - 75 fahrenheit? Or will they just be a bit slower similar to cubes?
Tbh, it's my first try with pans and I noticed alot that I would do different next time, so even if they won't fruit, I won't be that much disapointed, learnd alot. Will just hit up the next grains with that what I've got left on agar and do it better ;-)
Look at this post I made earlier this year.
Quote:
Baba Yaga said: First run with Nec-D fruited@spawn and uncased
coir/compost 50/50, 1:3 ratio @ 2-2.5 inch, temps 17C-22C with a couple of nights going down to 13-14C cause I forgot to switch the heater on. 130g wet which is a bit underwhelming but could have been in the 200s with a bit of grooming during colonization I suppose.

Happy as tbh. Since I only grew one pan cambo variety for years I was always curious how other pans would perform in a tub and this one seems to like it as well even F@S and not being cased didn't get in the way except from where the sub surface dried out in front of the bottom holes which I didn't mist during colonization. I am absolutely positive that this can be avoided by either dialing in air flow and/or misting. Those areas are actually not dead but are starting to pin now. Not sure if I wait for those to mature though.
Next tubs to be cased will be Wild Coast on a straw/compost/leached saw dust substrate. Colonized first but have given it full blast FAE for 2 days before casing.
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cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28076537 - 11/30/22 04:56 PM (1 year, 1 month ago) |
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Baby Yaga, I have been trying to figure out which pans yield those silvery caps so I can target them. Is that a Nec-D phenotype or am I hunting a silvery ghost caused by spore drops?
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#28076601 - 11/30/22 05:14 PM (1 year, 1 month ago) |
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The only thing that comes to mind when hearing silvery caps is Panealous bisporus.
Like in the photos hindsight posted a while back
Quote:
Baba Yaga said:
Quote:
Hindsight said: 

With bisporus I often think they look like they are made out of wax. Definitely my favorite to look at. Nice work.
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THE MYCOSMITH
Weekend mycologist


Registered: 01/24/22
Posts: 171
Last seen: 14 hours, 4 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28077286 - 11/30/22 11:02 PM (1 year, 1 month ago) |
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Ups sorry. Carry on and good Day.
Edited by THE MYCOSMITH (11/30/22 11:19 PM)
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goatboy666
Stranger



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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28078221 - 12/01/22 02:25 PM (1 year, 1 month ago) |
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I was looking at the pics in your profile and was going to ask.....what are those white pans? Look amazing. Never even heard of nec d.
Have a P.bisporus print that I've never got around to growing, you've inspired me to have a go of that. Same sub as pans? Heard people growing them on coir but i could be wrong?
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Edited by goatboy666 (12/01/22 02:28 PM)
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: goatboy666]
#28078304 - 12/01/22 03:08 PM (1 year, 1 month ago) |
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This would be Hindsight who is growing those leucistic Nec-D.
As far as I know Pan Bisp. is growing on the same stuff as other pans do.
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28078316 - 12/01/22 03:19 PM (1 year, 1 month ago) |
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Yes I'm growing those white NecD, though I am still trying to stabilize them and have not been having the best go of it. Having germination issues, along with cross-contamination by bisporus spores. Am going back to F1 now. Still keeping at it though.
Yes Bispos grow on same subs as pan cyans. I use horse manure / verm / straw in a 1:1:1 ratio..
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goatboy666
Stranger



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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#28078360 - 12/01/22 03:49 PM (1 year, 1 month ago) |
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Thanks, thats useful info.
Will keep my eye out for some spores. They looks really unusual. What issues are you having with stablizing?
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Egon_Spengler
Ever lurkin never workin



Registered: 04/02/22
Posts: 2,294
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Re: Official Copelandia/Panaeolus Thread [Re: goatboy666]
#28078847 - 12/01/22 09:42 PM (1 year, 1 month ago) |
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Evening, friends!
QQ: are Panaeolus cultures as prone to in vitro pinning on agar as cubensis cultures are, if left long enough?
Nearly every single one of my cube plates and nat plates will pin eventually. Is this as common with Pans?
My only bisporus cultures are pretty young at the moment, I’m mostly asking out of idle curiosity about what I may be able to expect down the line
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