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stagger



Registered: 05/05/19
Posts: 1,294
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Re: Official Copelandia/Panaeolus Thread [Re: MycoBrainz] 6
#28069018 - 11/25/22 03:23 PM (1 year, 2 months ago) |
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wow they move quick...first flush 110g dried to just under 7g
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Scotay
The Mad Mush Guy

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Re: Official Copelandia/Panaeolus Thread [Re: goatboy666]
#28069179 - 11/25/22 05:06 PM (1 year, 2 months ago) |
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Quote:
goatboy666 said: Pan.cyan 'Wild Coast. Grown on aged horse manure, straw. Popcorn spawn and a verm casing.


Heya Goat.... I like that little tote. What is the white material beneath it? Are you doing this with a smaller tub in a bigger one? Enquiring minds wanna know!! I hope all you panheads are doing well today!!!
Random question to whoever will chime in... When using grain water.... How much do you dilute it? I was thinking 50/50 with 1g agar per ml... Think that would be ok to pour in plates?
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay]
#28069225 - 11/25/22 05:40 PM (1 year, 2 months ago) |
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I've been using 1 part grain boil water to 2 parts water. I think that really depends on your grain water richness, and I am still experimenting with the ratios, but I also take the clarity and color of the solution into consideration. Going entirely by color alone is also a fine way to do it, since GW can change from batch to batch, and from grain to grain.
My first GWA attempt was whack. I was in need of germ plates, not prepped grain, so I did a quick simmer with a small amount of WBS, just for the the GW. It was very light in color, and made for great clarity. However there was such little nutrition in it that the mycelium was practically invisible and grew rather slow. I probably could have used that GW at 100% strength.
The next WBS grain prep gave me plenty of GW to work with, and I immediately noticed how much darker it was than the last attempt. After letting it cool, most of the sediment had settled. I poured 2 quarts through some paper towels, and it looked like common tea. (I have it frozen up in ice cubes waiting until I need to make more. Plus, now I have oat boil water in addition to the WBS boil water, so experiments shall continue.)
This resulted in a darker agar, but it's still translucent enough I don't mind. I really like how pan mycelium looks on it. It's so wispy that even I can spot contaminates if they are there. One of my pan bispo T2 plates was fully colonized in 8 days, which is a first for me.
As for the agar content, 1 gram per mL is nowhere close to right, but I assume you made a typo. 2% is pretty standard, and what I've currently been using. So if you are using 300 grams total water, (including grain water), multiply 300 by 0.02 = 6 grams agar.
Oh, and that white stuff is perlite.
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#28069227 - 11/25/22 05:43 PM (1 year, 2 months ago) |
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I run 3 parts grain water to 7 parts filtered water. Agar is 16 to 20 grams per liter. 20 for normal, 16 for spore germination.
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District
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#28069270 - 11/25/22 06:15 PM (1 year, 2 months ago) |
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hello fellas!
my first foray into a martha, with humidity and fae blown in from one tote. It´s a three rack size and only has one disc fogger, and cycles around 95% minus 5%.
Day 5. I thought i saw a knot from the side of the glass tray but it hasn't grown so I must be seeing things. Lol.
Concerned that maybe its the FAE. Right now the pc fan produces a very faint wind. If the pc speed is set higher the mist ceases to be mist_like but i´m concerned things would evaporate quickly. I have the trays down there so the mist falls gently on the casing.
It also doesn´t get that much outside air as I only have a window across the room open during the day, and the air quality is not ideal.
Anyways here it is. Thoughts?
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: District]
#28069311 - 11/25/22 06:51 PM (1 year, 2 months ago) |
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Looking pretty good overall! I do have some specific questions or points:
Do you have a catchpan under your martha? I think you're going to want one if you don't already have one.
I recommend cycle timers over humidity controllers, but others make it work.
Does your fogger tote lid seal well? You don't want any leaks there. It also looks like the water level is a little low. The more headspace you give it, the less fog you will get out the other end.
I also run a single disc fogger, but have found ways to optimize it's output.
Does your fogger fan come on with the fogger, or does it run constant? If you happen to have an aquarium heater that would fit in the fogger tote, I recommend using it and holding the water slightly warmer than your fruiting area. I aimed for 77 degrees or so for fruiting, but would keep my water around 80 to 82. That helped the single disc's output quite a bit, and I think it helps condense the fog on the fruits and casing layer, since it experiences a sudden temperature drop.
If you have a window even cracked a little bit, I think you'll have plenty of fresh air. I've ran with a nearby window open during summer, and with it shut during colder weather. I didn't see much difference in fruits that I could associate with the window.
I can't wait to see that full of fruits!
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Sillypj
Temporal Being

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Re: Official Copelandia/Panaeolus Thread [Re: District]
#28069361 - 11/25/22 07:24 PM (1 year, 2 months ago) |
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Quote:
District said: hello fellas!
my first foray into a martha, with humidity and fae blown in from one tote. It´s a three rack size and only has one disc fogger, and cycles around 95% minus 5%.
Day 5. I thought i saw a knot from the side of the glass tray but it hasn't grown so I must be seeing things. Lol.
Concerned that maybe its the FAE. Right now the pc fan produces a very faint wind. If the pc speed is set higher the mist ceases to be mist_like but i´m concerned things would evaporate quickly. I have the trays down there so the mist falls gently on the casing.
It also doesn´t get that much outside air as I only have a window across the room open during the day, and the air quality is not ideal.
Anyways here it is. Thoughts?

I would create another means of FAE in that tent. If not a bathroom fan, maybe build a vacuum tube at the bottom with another computer fan. Simply mount the fan on a section of that excess tubing and have it pull a vacuum of air out of the tent by having it at the bottom. A hepa filter from a vacuum cleaner could be added up top to allow fresh air to enter as the vacuum tube pulls out the humid air. That will get your humidity cycling and increase the fresh air.
Edited by Sillypj (11/25/22 07:25 PM)
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Sillypj]
#28069384 - 11/25/22 07:39 PM (1 year, 2 months ago) |
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If one wants to add a HEPA filter to a setup like this, I would suggest adding one to the intake of the fogger fan.
For one, it will actually help keep your fogger system clean much longer. It also will help keep your air and fog stream much cleaner than unfiltered room air. (Think about wanting to have super clean prints!)
If a HEPA is added to the tent itself with a fan pushing air out of the tent, air will take the path of least resistance and come in through the fogger system more readily than pass through a HEPA filter.
Even JakeonCid overlooked this design flaw on some of his earlier Jarthas. He mentioned a check valve that would prevent that from happening, but because of the direction of air flow, it wouldn't work that way.
Edited by ruawakeyet (11/25/22 07:44 PM)
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Sillypj
Temporal Being

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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#28069395 - 11/25/22 07:48 PM (1 year, 2 months ago) |
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Quote:
ruawakeyet said: If one wants to add a HEPA filter to a setup like this, I would suggest adding one to the intake of the fogger fan.
For one, it will actually help keep your fogger system clean much longer. It also will help keep your air and fog stream much cleaner than unfiltered room air. (Think about wanting to have super clean prints!)
If a HEPA is added to the tent itself with a fan pushing air out of the tent, air will take the path of least resistance and come in through the fogger system more readily than pass through a HEPA filter.
Even JakeonCid overlooked this design flaw on some of his earlier Jarthas. He mentioned a check valve that would prevent that from happening, but because of the direction of air flow, it wouldn't work that way.
Good idea. Maybe add the filter, then run the fan a bit more than just while the fogger is running.
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Sillypj] 3
#28069419 - 11/25/22 08:11 PM (1 year, 2 months ago) |
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A clean way I've found to add a HEPA filter to a fan intake, is to simply use a true HEPA air purifier as the filter and housing. The one pictured below worked perfectly.
I siliconed a 6" to 4" duct adapter to the housing, and ran 4" flex duct to a 4" inline booster fan with a fan speed controller to fine tune it. From there, it runs into the fogger of course. I think you can get adapters for those waterproof fans since you already have it. That would make connecting the flex duct to your waterproof fan clean and professional.
The HEPA filter kept my fogger system so clean I wouldn't have had to even clean it if it weren't for the steel parts rusting and contaminating the water. (I did add a little bit of hydrogen peroxide occasionally for sanitation and extra oxygen to the fruits.) I'm rebuilding my fogger system and making sure I don't use ANY steel parts anywhere, unlike my last build.
Even the steel wire inside dryer duct will rust. Steel screws will rust, etc. I'm switching all components to either plastic / PVC / silicone / or stainless steel. And I'm adding an auto-fill function with a larger reservoir, I'm going to permanently mount my fogger disc in the ideal location, and use some kind of foam to reduce the headspace as much as possible inside.

Edit: I don't have a picture of it because it got torn up in a move, but I even added an extra pre-filter for the air purifier housing.
To make one, you will need:
1x 14" x 14" x 12" box, available at WalMart (This specific box size could have been made for this exact purpose!)
1x 14" x 14" furnace filter (The quality is your choice. Since this is only a pre-filter, I went with a middle of the road quality filter.)
Packing tape
Duck tape
Silicone adhesive (Clear or red will work. The red adheres better, but the clear could probably be removed with a little bit of effort. That red silicone might be more permanent. Also your choice.)
To start, seal up all but one side of the box. Find one of the sides that measures 14" x 14", and cut off all but an inch or two of all four of the flaps. Fold the now shortened flaps inward. I'll come back to this part in a minute.
With the open 14" x 14" side facing toward you, (horizontally) determine the top and bottom of the box, and cut out a 6" hole directly in the middle of the top side. Angle your assembled air purifier through the hole. The adapter will now be sticking out the top, with the purifier inside the box. The adapter should be tight to the box. Seal that gap with silicone or hot glue. A little more silicone or hot glue on the bottom of the purifier will help it stay in place in the box. Just remember it will have to be disassembled someday to replace filters. (Maybe. The pre-filter works pretty well, and this air purifier is a 3 stage filter itself.)
Once you are satisfied with the fit of the purifier, it is now time to add the furnace filter. With those shortened flaps folded inward, the filter should fit snugly inside. You may have to wrap the outside edge of the furnace filter with duct tape until it fits snugly. It should take light pressure to press the filter in place. Once it is fit to the box, I seal that gap with packing tape. When it comes time to change out the filter, I'll cut the packing tape, replace the filter, and reseal it with tape again.
Make sure all the seams and corners of the box are sealed so dust doesn't bypass the filter. If you start off a brand new air purifier in a pre-filter like this, the expensive filters should last for years!
Edited by ruawakeyet (11/25/22 08:31 PM)
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District
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#28069549 - 11/25/22 10:24 PM (1 year, 2 months ago) |
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Thanks for the input! Adding heater now.
- the fan runs constant. Not sure if it’s enough for fae as it is in the lowest setting possible.
- I could increase that fan speed for more FAE but the mist coming out would be diluted with the wind as opposed to a fine jet of mist. Thoughts?
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District
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Re: Official Copelandia/Panaeolus Thread [Re: District]
#28069555 - 11/25/22 10:30 PM (1 year, 2 months ago) |
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Exhaust. The rack actually is too tall for the tent. So I added a plastic “skirt” (not seen) for it too reach the Floor.
I could add a ip67 PC fan on the Floor and cut out the skirt as exhaust opening and set to a timer. Not sure yet.
Thanks again.
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: District]
#28069560 - 11/25/22 10:35 PM (1 year, 2 months ago) |
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Quote:
District said: - the fan runs constant. Not sure if it’s enough for fae as it is in the lowest setting possible.
- I could increase that fan speed for more FAE but the mist coming out would be diluted with the wind as opposed to a fine jet of mist. Thoughts?
I never had constantly moving air, so I think you'll be ok. My system would run for about 5 minutes, gentle air flow with fog, then shut off. My heater would then kick on and raise the temp in the tent, but no fae occurred for approximately 7 to 10 minutes until the cycle repeats.
That is as much help as I can offer without knowing the answers to some other questions I asked.
My main question now is how air-tight is the fogger lid? You can get more fog without leaks, and by reducing that headspace by filling it up more.
You're also going to want to get some kind of base to catch all the condensation that will likely happen. I've seen most people use a washing machine tray.
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District
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#28069591 - 11/25/22 11:02 PM (1 year, 2 months ago) |
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Quote:
ruawakeyet said:
My main question now is how air-tight is the fogger lid? You can get more fog without leaks, and by reducing that headspace by filling it up more.
You're also going to want to get some kind of base to catch all the condensation that will likely happen. I've seen most people use a washing machine tray.
The tote is sealed with a weather strip for a gasket on the inside of the lid and it has clasp. Not perfect though as I manhandled this tote in its previous life.
As the water lever decreases, so too does the quantity of fog. Ill elevate it further a full 3-4 inches.
I have condesation on the faux wood floor. I was just going to wipe it down. Ill probably get aluminum tray and glue it together for the drip tray but that wont be until I go to the grocery or hardware store.
Thanks!
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goatboy666
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps]
#28069809 - 11/26/22 07:28 AM (1 year, 2 months ago) |
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Yeah, i have a few spare swabs if you want to have a look at them through a scope
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goatboy666
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay]
#28069813 - 11/26/22 07:33 AM (1 year, 2 months ago) |
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Its perlite bro, to keep the humidity up. So its just a little tub in a bigger one with the lid on. I open it daily and give if a waft for some fresh air.
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District
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Re: Official Copelandia/Panaeolus Thread [Re: goatboy666] 1
#28070698 - 11/26/22 08:04 PM (1 year, 2 months ago) |
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Well six days in and no primordia. Im going to increase the fan speed a couple notches for fae and supplement with spraying the casing daily.
I figured it is fae cuz I have an oyster that is fruiting (I grow in the balcony) so I put it in the Martha and saw this morning it was getting long necked.
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Scotay
The Mad Mush Guy

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Re: Official Copelandia/Panaeolus Thread [Re: goatboy666]
#28070738 - 11/26/22 08:34 PM (1 year, 2 months ago) |
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Quote:
goatboy666 said: Its perlite bro, to keep the humidity up. So its just a little tub in a bigger one with the lid on. I open it daily and give if a waft for some fresh air.
Thanks for taking the time to answer that goat!! Hope your weekend is great!!! Hello all you panheads friends and neighbors!! 😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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Shrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] 1
#28071011 - 11/27/22 01:19 AM (1 year, 2 months ago) |
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Here a quick update on my two (Cop.cyan. "Jamaica") tubes:
11L Shoebox with my wpoo "experiment" spawned 14.11 (following babayagas Thread:

31L Monotube with normal hpoo spawned 21.11 following asuras Thread:

I think due to the low temperatures (21°C-23°C) the colonization is taking a bit longer. Also my Wpoo experiment had a bit a thick toplayer, so I scratched a bit of on the 21.11 and since then its recovering quite well. I think my Wpoo thingi was maybe a little bit too wet, what also seems to be adding to the longer colonization time 
I think I will wait a couple of days longer until I apply the casing layer, or what do you pan-veterans think about that?
Edited by Shrimps (11/27/22 01:21 AM)
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goatboy666
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] 3
#28071023 - 11/27/22 01:36 AM (1 year, 2 months ago) |
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You are welcome, the perlite is also quite damp. Heres a better picture...
Have a great day 😀
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