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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: PanMax] * 1
    #28014221 - 10/24/22 03:01 PM (1 year, 3 months ago)

Doing your own sub and grain might solve your problem. Pasteurization is easy but you might need to have a few goes at it to get it down. Have you decided what substrate to use yet? I assume that you are germinating and cleaning up your cultures on agar.


You can put the next batch in the tent, no problem and you can harvest fruits. Just keep em separate and make a tea when you are dosing and all will be fine. If you are feeling too uncomfortable consuming those then chuck em in the bin. Nothing worse than starting to freak out over whether the fruits were safe to consume or not when tripping. Should be good though.

How long to keep them around for is totally up to you. Take em out when they have stopped growing but if you decide to not harvest any then you might get rid of them now :shrug:. I personally would put those trays in a mono and keep them around just to see if and when they start turning green...to gather some data so to say.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: PanMax] * 2
    #28014246 - 10/24/22 03:15 PM (1 year, 3 months ago)

Quote:

PanMax said:


So as time goes on, I think you are absolutely correct. Which is a real shame because this would have been an amazing harvest.






What I forgot to say is that I find the density of the pin set alone very suspicious. It never ended well when I had fruits coming in as packed clusters like this.

Here a few of my examples that never made it to maturity and aborted or stalled:



Edited by Baba Yaga (10/25/22 07:10 PM)


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OfflinePanMax
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28014272 - 10/24/22 03:27 PM (1 year, 3 months ago)

And what did you do with those pin sets/ mini fruits?

And my sub mixture is 1 part hpoo, 1 part Vern, 1/2 part straw, and 1/2 part coir.  I put it in a 165 degree for 4 hours total. It takes about 1.5 hours for the center to reach temp. Then I let it pasteurize for another2.5 hours.

My grain is now all white millet sterilized in a PC for 90 minutes.


--------------------
Forgive me, I’m just learning


Edited by PanMax (10/24/22 03:33 PM)


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28014274 - 10/24/22 03:28 PM (1 year, 3 months ago)

:takingnotes:


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: PanMax] * 1
    #28014423 - 10/24/22 04:35 PM (1 year, 3 months ago)

Quote:

PanMax said:
And what did you do with those pin sets/ mini fruits?







Already have too much and quite a bit of what I grow goes on the compost pile :dead:


Quote:

And my sub mixture is 1 part hpoo, 1 part Vern, 1/2 part straw, and 1/2 part coir.  I put it in a 165 degree for 4 hours total. It takes about 1.5 hours for the center to reach temp. Then I let it pasteurize for another2.5 hours.

My grain is now all white millet sterilized in a PC for 90 minutes.





Sounds like a solid prep. Are you cleaning your cultures up on agar?


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OfflinePanMax
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28014441 - 10/24/22 04:41 PM (1 year, 3 months ago)

I am....now.  unfortunately, the set I just put to fruit was the last of my mixed culture, AND using the vendors grain.  I just put some single culture grain to sub.

As an aside, I had two bags of their grain left over.  One of them started to turn grey and humid IN THE BAG.  I threw them both away.  Won't be buying anything from them again.  Besides, better off making it myself.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: PanMax] * 2
    #28014458 - 10/24/22 04:49 PM (1 year, 3 months ago)

That makes it very likely that contam is causing this.

Hope your agar work and home made substrate is going to improve your grows in the future. 

DIY FTW


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 3
    #28014677 - 10/24/22 06:27 PM (1 year, 3 months ago)

Today I spawned RDU to coir/compost. Not too sure about this culture but I had to give it a go.

Just finished setting up the SAB to get some clone LC on the way.



These two clones on 1% LME agar will be dropped to 0.1% LME broth. Left Nec-D, right Bunnell. Clones are looking so much more organized on agar than MS.





The bottle with a baggy on top is chugging along. After the first flush only a couple of pans popped so I call this flush 2.5.



Edited by Baba Yaga (10/24/22 11:52 PM)


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28014835 - 10/24/22 07:38 PM (1 year, 3 months ago)

Sweet! I like the looks of your agar containers, I believe I'm going to have to source some of those for myself. I'm over using jars, for so many reasons!


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 2
    #28015177 - 10/24/22 10:04 PM (1 year, 3 months ago)

I love those 2oz condiment cups and use them as no pours. 300ml of agar is plenty for 25 cups which is the amount that I can sterilize in my 8 liter prestige high dome PC. Running them in a smaller cooker is super fast compared to a 23qt presto.

The cups are only 60mm in diameter but I find them big enough for my needs, probably because I rarely inoculate with wedges. They work just as good for me as regular petris, no increase in contam rate. I do wipe them clean before going into the SAB which is a bit more work but they don't need to be wrapped. I bought 600 of these but they last a long time if you get good quality, sturdy ones and I not even have used 100 in the last couple of years. I mean I don't grow all year but if I would I'd say 100 a year is plenty.


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OfflineSillypj
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox] * 2
    #28015673 - 10/25/22 09:50 AM (1 year, 3 months ago)

Automated pasteurizer is a game changer..

I learned a lot from building this thing.  One, the core temp of a decent bag of substrate takes about 6 hours in 150deg water to reach a core temp of 150deg.  I have a wireless meat thermometer set up with two probes.  I can remotely monitor the temps of them at any time.  However after multiple runs I have found that 8hrs is pretty much a sure thing to reach 150deg overall temp without monitoring. The best part is that the temp never exceeds 150deg throughout, so no harm is done to the "friendlies", and you can use it right away after cooling (which decreases time waiting to regrow those "friendlies")

The set up was super simple and not too expensive. Once it reaches temp it only "tops off" with heat every half hour or so for a couple mins, its pretty thermally efficient.

1 - Penn A421 Controller $85 (Comes with accurate temp probe)
1 - Bucket Heater $25
1 - Extension Cord - $15 (cut one end off)
1 - Cooler (any size)
1 - Wire Rack $15

Fill it with water, throw in the bags, set it to 150deg, and wait 8hrs..



--------------------



Edited by Sillypj (10/25/22 10:21 AM)


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OnlineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Sillypj]
    #28015677 - 10/25/22 10:02 AM (1 year, 3 months ago)

150F is 65C. That doesn't sound sufficient to me. I tend to get gray mold when I under-pasteurize. Though it's true that gray molds eventually appear anyway if you let the sub sit long enough.


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OfflineSillypj
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #28015682 - 10/25/22 10:11 AM (1 year, 3 months ago)

Quote:

Adas said:
150F is 65C. That doesn't sound sufficient to me. I tend to get gray mold when I under-pasteurize. Though it's true that gray molds eventually appear anyway if you let the sub sit long enough.




I think the gold standard is 140F to 160F(Max).  I have been running it to turn on at 145F (or lower) and turn off at 150F.  You can easily set that higher with a quick toggle of the button.  I have not had any issues getting multiple flushes out of cakes using the 150F setting, that photo was taken in April of this year, so I would estimate 5 batches ran using 150F as the setting.


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OnlineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Sillypj]
    #28015685 - 10/25/22 10:15 AM (1 year, 3 months ago)

I'm not talking about flushes. Flushes are a function of your species and conditions and what not. I'm talking about storage of pasteurized sub.


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OfflineSillypj
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #28015688 - 10/25/22 10:17 AM (1 year, 3 months ago)

Quote:

Adas said:
I'm not talking about flushes. Flushes are a function of your species and conditions and what not. I'm talking about storage of pasteurized sub.




Ahh.. I get you.  What temp do you find best for longevity while maintaining good "friendly" count?


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Re: Official Copelandia/Panaeolus Thread [Re: Sillypj]
    #28015700 - 10/25/22 10:22 AM (1 year, 3 months ago)

I'm not super exact with my pasteurization but around 72Β°C seems best. Not sure if we can talk about "friendly" bacteria here, because if that was relevant, I'd imagine they would protect the sub from mold contamination, which they obviously don't. :shrug:


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OfflinePanMax
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 8
    #28015716 - 10/25/22 10:31 AM (1 year, 3 months ago)



Well, these are the final pictures. I decided to go ahead and cut these off and dry them, then chuck it. I didn’t see any obvious contamination, and someone else said it may have been a bad genome. This batch came from a spore syringe, so it wasn’t a single culture. 

On another note, since I wasn’t worried about another flush, I just kind of went at it to get what fruits I could, but this thing would have been a nightmare to harvest if I was trying to be gentle and get more flushes. What have you guys found to be the best way to harvest when you get a β€œcanopy”-like coverage.

Thoughts? Think the fruits are still safe?


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Sillypj] * 3
    #28015729 - 10/25/22 10:38 AM (1 year, 3 months ago)

Quote:

Sillypj said:
Quote:

Adas said:
I'm not talking about flushes. Flushes are a function of your species and conditions and what not. I'm talking about storage of pasteurized sub.




Ahh.. I get you.  What temp do you find best for longevity while maintaining good "friendly" count?




I went from 145 to 165 and the difference was night and day. 160-165F is all I do anymore. I build a similar setup as you but used steam instead of a water bath. Link in my sig.


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Offlinemoonwatcher
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 2
    #28015741 - 10/25/22 10:55 AM (1 year, 3 months ago)



Check out my mighty BVI flush. Time to get the dehydrator ready!


Edited by moonwatcher (10/25/22 10:58 AM)


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OfflineSillypj
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28015747 - 10/25/22 11:01 AM (1 year, 3 months ago)

Quote:

Hindsight said:
Quote:

Sillypj said:
Quote:

Adas said:
I'm not talking about flushes. Flushes are a function of your species and conditions and what not. I'm talking about storage of pasteurized sub.




Ahh.. I get you.  What temp do you find best for longevity while maintaining good "friendly" count?




I went from 145 to 165 and the difference was night and day. 160-165F is all I do anymore. I build a similar setup as you but used steam instead of a water bath. Link in my sig.




Looks like a trial is in my future.  I will bump it up a bit in my next run.


--------------------



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