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Invisibleruawakeyet
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Registered: 05/03/21
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Re: Official Copelandia/Panaeolus Thread [Re: PanMax]
    #28010149 - 10/22/22 12:54 AM (1 year, 3 months ago)

My eyesight isn't what it used to be. Congrats! I'm stoked for you!


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Invisibleekabpils
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 9
    #28010166 - 10/22/22 01:35 AM (1 year, 3 months ago)

First wave of Brooksville:



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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
    #28010183 - 10/22/22 02:54 AM (1 year, 3 months ago)

Quote:

ekabpils said:
First wave of Brooksville:






Very nice flush! :raisemyglass:


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OfflineVast218
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28010508 - 10/22/22 10:46 AM (1 year, 3 months ago)

Is this primordia?



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Offlinemoonwatcher
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
    #28010546 - 10/22/22 11:25 AM (1 year, 3 months ago)

Quote:

ekabpils said:
First wave of Brooksville:






Excellent as always. Is this just a bubbler in the water cup, I don't see a heater?


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Vast218]
    #28010551 - 10/22/22 11:29 AM (1 year, 3 months ago)

Quote:

Vast218 said:
Is this primordia?






I can't tell from the photo, but it could be mycelium / overlay, or contamination. Without a microscope, I think all you can do is keep a close eye on it at this point. Be ready to pitch it if any of it starts to turn green.


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OfflineVast218
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #28010560 - 10/22/22 11:36 AM (1 year, 3 months ago)

Thanks. No green so far but the blue-ish grey seems to be the normal mycelium color for this species


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Offlinemoonwatcher
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Re: Official Copelandia/Panaeolus Thread [Re: Vast218] * 1
    #28010571 - 10/22/22 11:46 AM (1 year, 3 months ago)

Quote:

Vast218 said:
Is this primordia?






Are you using some sort of coir casing? Or is that the uncased surface? In either case, doesn't look right to me for sure. Are you sure your spawn was clean and good?


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OfflineVast218
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Re: Official Copelandia/Panaeolus Thread [Re: moonwatcher]
    #28010598 - 10/22/22 12:04 PM (1 year, 3 months ago)

Quote:

moonwatcher said:
Quote:

Vast218 said:
Is this primordia?






Are you using some sort of coir casing? Or is that the uncased surface? In either case, doesn't look right to me for sure. Are you sure your spawn was clean and good?




Coir casing yes


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Vast218]
    #28010614 - 10/22/22 12:13 PM (1 year, 3 months ago)

I should have caught that, but good thing moonwatcher did.

Peat moss and verm (with some pickling lime) is typically used for the casing layer. Coir is avoided because it usually ends up colonized, which is what you might be seeing. Or it's still contamination...


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OfflineVast218
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #28010664 - 10/22/22 12:40 PM (1 year, 3 months ago)

Quote:

ruawakeyet said:
I should have caught that, but good thing moonwatcher did.

Peat moss and verm (with some pickling lime) is typically used for the casing layer. Coir is avoided because it usually ends up colonized, which is what you might be seeing. Or it's still contamination...




Ok, I need to improve my casing recipe. How much of the three ingredients would you use roughly? Like 50/50 with a bit of lime mixed in?

I’ll keep an eye on this growth anyway and see how it goes


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Vast218]
    #28010842 - 10/22/22 02:40 PM (1 year, 3 months ago)

Quote:

Vast218 said:Ok, I need to improve my casing recipe. How much of the three ingredients would you use roughly? Like 50/50 with a bit of lime mixed in?

I’ll keep an eye on this growth anyway and see how it goes




Yeah, 50/50 is commonly used. I recommend Hindsight's or Fahtster's casing recipe. Hindsight covered exactly what he does for casing a few pages back. I know Fahtster has a detailed write-up, he uses closer to 60/40, so a little less verm is used. Since I think my past casing recipe ended up with too much verm, I'm trying his recipe next. (Edit: I know Fahtster is a master cube grower, so his casing is intended for cubes, but I don't think Pans will mind one bit. The casing layer thickness should be thin for pans, like not even 100% coverage. More like 95% coverage, and as thin as possible.)

Welcome by the way! I'm stoked to see so many new Pan growers, I can't keep up with them all!


Edited by ruawakeyet (10/22/22 02:42 PM)


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OfflineVast218
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #28010958 - 10/22/22 04:04 PM (1 year, 3 months ago)

Quote:

ruawakeyet said:
Quote:

Vast218 said:Ok, I need to improve my casing recipe. How much of the three ingredients would you use roughly? Like 50/50 with a bit of lime mixed in?

I’ll keep an eye on this growth anyway and see how it goes




Yeah, 50/50 is commonly used. I recommend Hindsight's or Fahtster's casing recipe. Hindsight covered exactly what he does for casing a few pages back. I know Fahtster has a detailed write-up, he uses closer to 60/40, so a little less verm is used. Since I think my past casing recipe ended up with too much verm, I'm trying his recipe next. (Edit: I know Fahtster is a master cube grower, so his casing is intended for cubes, but I don't think Pans will mind one bit. The casing layer thickness should be thin for pans, like not even 100% coverage. More like 95% coverage, and as thin as possible.)

Welcome by the way! I'm stoked to see so many new Pan growers, I can't keep up with them all!





Thank you!

Though in fairness I can’t really call myself a pan grower, because I’ve never grown a single pan πŸ˜‚. But I found this pan in the wild a while back and I’ve become obsessed with the idea of breeding it, no luck so far but I’m not giving up.


Edited by Vast218 (10/22/22 04:07 PM)


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Vast218]
    #28010969 - 10/22/22 04:17 PM (1 year, 3 months ago)

You'll get there. I've heard starting from a wild print can be very challenging. I can see it being a challenge without any domestication at all, plus prints taken from wild fruits are probably dirty as hell compared to an indoor grown fruit. (Possibly, it all depends on a lot of variables.)

You're in the right place, just keep reading and you'll dial them in. If you want to share the steps you've taken so far, we can try and provide feedback or suggestions. I would offer you a print if I had any extras. (Future goals)


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OnlineSirPsycho
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 4
    #28011034 - 10/22/22 05:19 PM (1 year, 3 months ago)


Pan cyan huasteca. Inoculated in anger, which I believe aided with speedy germination.


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Offlinethe man
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Re: Official Copelandia/Panaeolus Thread [Re: SirPsycho]
    #28011221 - 10/22/22 07:17 PM (1 year, 3 months ago)

you folks find pan bisporus to need less babying? ie more of cube temps humidity and curious if it fruits with cube teks as doesnt grow from dung like cyans so may not need anything other than grains and coir. prob have to test it out but thought id see what u guys have found. cheers!


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: SirPsycho] * 1
    #28011611 - 10/22/22 10:35 PM (1 year, 3 months ago)

Quote:

SirPsycho said:Pan cyan huasteca. Inoculated in anger, which I believe aided with speedy germination.




LOL, I can relate!

I just transferred some Peace River, the last of the transfers were done in anger, so I hope it helps the mycelium speed up!

8 days ago, I swabbed 3 plates and didn't find anything fast at all. I remember Peace River looks different every time I've had it on agar, but I really don't like seeing aerial mycelium like I have. I wouldn't be as worried about it if it wasn't so freaking slow.

I also swabbed 3 plates of Alabama and got nothing. I can see some spores in places where I swabbed, but not even a single contaminant has germed. (At least I can use a SAB properly now.) Anyone got any tricks to try to get them to germ?

What really ticked me off tonight is that I absolutely hate my cheap ass methods of working agar. The transfers don't want to come off the tip of the knife, and using jars for plates don't allow you to get the angle you need. I've got some tool hunting to do in order to find a work around, because I don't like getting pissed off doing something I should enjoy. I'm also open to any tips anyone might have if they also use 1/2 pint wide mouth jars for agar plates. I might try a sterile syringe with some air inside and use the needle to barely stick the segment being transferred, then use the air to push it off the needle. (Edit: This was one of my worst ideas ever. The plug always shot off like a rocket. It's like one of those handheld potato plug shooters.) I've been playing with different methods since my first germ plate was too clustered to separate much, at least it's good for practice.


Edited by ruawakeyet (10/23/22 01:14 AM)


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OfflinePanMax
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 5
    #28011651 - 10/22/22 11:18 PM (1 year, 3 months ago)

How it’s going….



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OfflineScotay
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #28011669 - 10/22/22 11:28 PM (1 year, 3 months ago)

Happy Saturday/Sunday folks!! I've tried a couple different recipe for agar and I can't get the pans to start there. I made up a batch of ten plates and 7 I scratched the print directly onto the agar and nothing grew. The other 3 I tried a cube print and all 3 of them popped. I'm not actually going to put it to grain. Just wanted to see if I'm fuckin it up or is the pans being finicky? I've had more luck squirting syringe to grain. Not really sure what the deal is. I will get this figured out!!! Hope everyone is having a good weekend!!😎✌️


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] * 3
    #28011717 - 10/22/22 11:48 PM (1 year, 3 months ago)

The Man, I haven't grown bisporus, so I'm refraining from answering. I did want to say welcome! If you haven't searched this thread already, try that while waiting for a reply. Make sure you check out Adas's work with bisporus! (And plenty of others!)

Pan Max, that's looking killer. I'm still not used to seeing that trait in pans, but now I see how that white ring is more pronounced on only some of them. I'm looking forward to seeing it in another day or two!

Scotay, I am currently experiencing the same thing as you, check out my reply above if you want. I would rule out your agar as long as that cube is eating it up. I also have a pearl oyster on this same batch as agar, and it grew out really nice and quick. I know oyster is fast as shit, but it had no problem reaching the walls 8 days after transfer. I know my agar is good enough, I should have better results from my pan cultures. (This batch could also be better, 8 days is a little slow for oyster, IMO.)

I don't have any definite answers other than it seems pan spores have a limited shelf life. Sometimes they might just be dead by the time we get them on agar. I'm going to experiment a little tonight since I have 3 germ plates to play with. I'm going to warm one of them up and let it stay warm overnight in the dehydrator. I'm going to try around 90 degrees F, I'll give an update with any results.


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