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OfflineSillypj
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Registered: 09/19/22
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Last seen: 5 months, 22 days
Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox] * 6
    #27999364 - 10/14/22 07:39 PM (1 year, 3 months ago)

Here's a BVI run in a smaller setup I'm running.  This looks nice, but is actually tiny as hell. 



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Invisibleruawakeyet
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Registered: 05/03/21
Posts: 1,862
Re: Official Copelandia/Panaeolus Thread [Re: Sillypj]
    #27999391 - 10/14/22 07:50 PM (1 year, 3 months ago)

Hopefully, those are the first of many pins to form. I like how they are near the center of the tray instead of the side, I think that's a good indicator that your conditions are close to ideal. When my conditions were lacking in the past, I would see more pins from the sides of the trays. That could just be anecdotal, but I'm excited to see what happens from here on!

Those BVIs are packed in there! I dread picking those tiny little fuckers, which is why I do everything I can to get bigger pan fruits. I'll automate, build, DIY, I'll do anything and everything I can afford to do in order to grow them as big as I can get them. (Other than stealing or some other dumb shit like that.)

Once I got past having nothing but tiny fruits, growing pans finally became worth it for me. I seriously questioned it for a bit, and it was touch and go there for a bit!


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OfflineSillypj
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Registered: 09/19/22
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #27999424 - 10/14/22 08:00 PM (1 year, 3 months ago)

Quote:

ruawakeyet said:
Hopefully, those are the first of many pins to form. I like how they are near the center of the tray instead of the side, I think that's a good indicator that your conditions are close to ideal. When my conditions were lacking in the past, I would see more pins from the sides of the trays. That could just be anecdotal, but I'm excited to see what happens from here on!

Those BVIs are packed in there! I dread picking those tiny little fuckers, which is why I do everything I can to get bigger pan fruits. I'll automate, build, DIY, I'll do anything and everything I can afford to do in order to grow them as big as I can get them. (Other than stealing or some other dumb shit like that.)

Once I got past having nothing but tiny fruits, growing pans finally became worth it for me. I seriously questioned it for a bit, and it was touch and go there for a bit!





Cool! I'll update again as things (hopefully) develop.  These things are well worth the extra effort for sure. Night and day with cubensis.


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Invisibleruawakeyet
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Registered: 05/03/21
Posts: 1,862
Re: Official Copelandia/Panaeolus Thread [Re: Sillypj] * 1
    #27999439 - 10/14/22 08:09 PM (1 year, 3 months ago)

Quote:

Sillypj said:These things are well worth the extra effort for sure. Night and day with cubensis.




Yes they are! I honestly can't wait for cubensis to take the back seat in active mycology.


Hey Ghia, I'm sorry our discussion got so fucked today. I apologize to you and everyone else that ignored the entire thing.

Let's get back to cultivation. It looks like you're running straight verm for your casing layer. Have you tried 50/50 casing mix? It's something to consider for your next run.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Sillypj] * 3
    #27999515 - 10/14/22 08:56 PM (1 year, 3 months ago)

Quote:

Sillypj said:
Here's a BVI run in a smaller setup I'm running.  This looks nice, but is actually tiny as hell. 






BVI is always tiny in my experience. 2nd and 3rd flushes are larger than the first but still small when compared to other pans. They pack a punch though. Right up there with NecD.


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OfflinePanMax
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Registered: 09/14/22
Posts: 53
Last seen: 8 days, 17 hours
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27999549 - 10/14/22 09:13 PM (1 year, 3 months ago)

Quote:

Hindsight said:
I do the same, though I go to agar instead of grain to revive - just to ensure cleanliness but I'm probably going to stop doing that because I haven't had a single issue yet. I have created quite a library of clone cultures and it keeps growing.






OK, what is this??  So just agar or grain to sterile water in 2ml vials??  How do you sterilize the water in the vials?  Put the whole thing in the PC

More info please!


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Forgive me, I’m just learning


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InvisibleBaba Yaga
♄ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: PanMax] * 2
    #27999772 - 10/15/22 12:42 AM (1 year, 3 months ago)

Quote:

PanMax said:
OK, what is this??  So just agar or grain to sterile water in 2ml vials??  How do you sterilize the water in the vials?  Put the whole thing in the PC

More info please!




Here is one write up about it:

https://www.shroomery.org/forums/showflat.php/Number/27778467


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Invisiblecambocyan

Registered: 06/24/20
Posts: 74
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 8
    #27999897 - 10/15/22 05:18 AM (1 year, 3 months ago)

Wild Coast
sunny side up ā˜€ļø



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OfflineRusty2096
rah rah raw in Lady gaga
I'm a teapot User Gallery


Registered: 08/23/22
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Re: Official Copelandia/Panaeolus Thread [Re: cambocyan] * 1
    #27999900 - 10/15/22 05:20 AM (1 year, 3 months ago)

Quote:

cambocyan said:
Wild Coast
sunny side up ā˜€ļø






Gonna have 3 sunny side up with bacon šŸ„“ for breakfast? šŸ˜‹


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: PanMax] * 3
    #27999960 - 10/15/22 06:45 AM (1 year, 3 months ago)

Quote:

PanMax said:
Quote:

Hindsight said:
I do the same, though I go to agar instead of grain to revive - just to ensure cleanliness but I'm probably going to stop doing that because I haven't had a single issue yet. I have created quite a library of clone cultures and it keeps growing.






OK, what is this??  So just agar or grain to sterile water in 2ml vials??  How do you sterilize the water in the vials?  Put the whole thing in the PC

More info please!




Yeah the link Baba posted is perfect. Only thing I can add to it is:
1. Here is the vial holder I use: https://www.amazon.com/gp/product/B07D2ZV3Q2
2. Here are the vials I like: https://www.amazon.com/gp/product/B06XHNT1XK
3. Some people have said you should only put mycelium in the vial with the water (IE they say to scrape some mycelium off the top of the agar plate and put that in). I don't do that. I just cut a little piece off the plate like I would when doing any old transfer, though I tent to make it a smaller than normal piece.
4. When ready to pull some of you culture from the vial, I like to shake it vigorously which helps break up the mycelium and distribute it throughout the distilled water. From here, you can simply dip a sterilized loop into it and then swipe it on a plate, or you can pull some up with a clean syringe. I like the loop method the best because it uses the least amount of your source material.

Beautiful tray Cambocyan! Those are huge caps.


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OfflinePanMax
FNG

Registered: 09/14/22
Posts: 53
Last seen: 8 days, 17 hours
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27999996 - 10/15/22 07:24 AM (1 year, 3 months ago)

Thanks Baba and Hindsight.

Are the caps on the vial loose when PC’ing them?

Do you use one of those little lab tools to fill them?


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: PanMax] * 3
    #27999999 - 10/15/22 07:29 AM (1 year, 3 months ago)

I have left the caps a little loose and also tightened them fully and don't notice a difference so now I just tighten them. You should also check them for tightness after the PC cycle because the caps tend to loosen a bit. I just use a syringe to fill them. Pour some distilled water into a cup, then pull it up into a syringe and start squirting it into the vials while they are sitting open in the holder. It doesn't have to be exact - just eyeball it. I do it like a bartender pours a row of shots on the bar - just get the water everywhere lol. Whatever gets it done fastest. Then cap the vials, and set the whole holder into the pressure cooker.


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OfflinePanMax
FNG

Registered: 09/14/22
Posts: 53
Last seen: 8 days, 17 hours
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28000003 - 10/15/22 07:32 AM (1 year, 3 months ago)

Can you do a bunch and just have them ready to use, or just prepare the ones you plan to use right away?


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OfflineRusty2096
rah rah raw in Lady gaga
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Registered: 08/23/22
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Re: Official Copelandia/Panaeolus Thread [Re: PanMax] * 1
    #28000011 - 10/15/22 07:51 AM (1 year, 3 months ago)

Quote:

PanMax said:
Can you do a bunch and just have them ready to use, or just prepare the ones you plan to use right away?




This may sound rude to you but trust me it isn't : the fact that you asked that question clearly shows that you are not ready to start long time storage of worthy cultures.

Read the basic threads ands teks. Cult some mushrooms. Cult some more. Learn agar, learn to clone and to select that close. Grow these clones.

Expand your collection.

Then and only then, IMO, come back to ask more long term storage questions if you still have any (but I bet you won't).

Again, I'm not giving you shit here but you legit asked 3 basic questions in a row about something that will, at the moment, be useless to you. Learn to walk before you learn to run.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)


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OfflineFridgedoor
Psssssst!
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Registered: 09/13/20
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Re: Official Copelandia/Panaeolus Thread [Re: Rusty2096]
    #28000037 - 10/15/22 08:35 AM (1 year, 3 months ago)

Hi everyone, some time ago I tried my hands on growing pans. I got everything right accept for the pasteurization of hpoo and lost everything to trich. So I  concentrated on species that do not require hpoo as part of the substrate...

Now I have some good looking pan spawn available which should be enough for 3 mini monos (I like Baba Yaga's way of doing this).

I just need to get the pasteurization procedure right. Looking at Franks tek , I might go that route. I really would like to get at least one of the 3 tubs to be a success.

There are a couple of questions on my mind, but one is: can I pasteurize the hpoo only and do the coir/verm separately via the bucket tek? Or is it best to mix everything and pasteurize everything together? I am asking as I can only process a little in one go and it would help if it's ok to prepare coir/verm and hpoo separately.


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Hokus Pokus Fidibus!


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: PanMax] * 4
    #28000040 - 10/15/22 08:38 AM (1 year, 3 months ago)

Quote:

PanMax said:
Can you do a bunch and just have them ready to use, or just prepare the ones you plan to use right away?




I store them on the side of my desk of my flow hood, sitting in the holder. They sit there for months, getting used as I need them. When I pop one open for long term storage, after it is capped and labeled, I put it into a different holder that I have sitting in a desk drawer (cool dark place). I also use the vials when I need sterile water for any purpose. One example is when I put spores from a foil spore print to agar - I will open a vial, dip a sterile loop into the sterile water, then rub that on my foil print. It makes more spores stick to the loop. Another example is making spore solution from spore swabs - I use Fahtster's method: I will open 2 vials, put a clean swab and a spore swab together into the first vial and rub them together twice as a rinse, then I will move the swabs to the second vial and rub them together until there is no more color on the spore swab. What is left in the vial is now the spore solution. I discard the contents of the first vial and use the contents of the second as the spore solution, either by just pouring it onto a plate, pulling it up into a syringe, or dipping a loop into it and streaking the loop on a plate.


@FridgeDoor: What you are asking will work fine. I suggest 160F core temp for 2 hours. Take a look at my steam pasteurizer in my journal.


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OfflineFridgedoor
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28000063 - 10/15/22 08:57 AM (1 year, 3 months ago)

Awesome thanks, Hindsight! That's good to hear. I will check out your steam sterilizer now :smile:.


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Hokus Pokus Fidibus!


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: cambocyan]
    #28000066 - 10/15/22 08:58 AM (1 year, 3 months ago)

Quote:

cambocyan said:
Wild Coast
sunny side up ā˜€ļø






That sub looks really shallow, is that an optical illusion? If it is, you still pulled a great flush! Are you chasing the best BE possible?


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OfflineYoshiTrainer
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 10
    #28000665 - 10/15/22 05:08 PM (1 year, 3 months ago)

More 'Hunter Valley' Australia.



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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
    #28000696 - 10/15/22 05:28 PM (1 year, 3 months ago)

Darn, that's not all I see there...

Those Hunter Valleys look great, and I like the drip irrigation you have for it.


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