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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 8
#27982990 - 10/05/22 03:15 PM (1 year, 3 months ago) |
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Alright here the last of the Cased/Uncased Wild Coast MS tub. No real difference that stands out much and since this is a multi spore grow I would call it even.
Wild Coast MS, coir/compost 50/50, 1:3 @ 2,5", fully colonized after 8 days, fruited uncased after 9 days, cased half the urface after 14 days.
Today after 20 days.

They are just about to drop spores so it's time to take some prints and clones. Much fatter than the Bunnell and Nec-D so far but I also had the sub moisture dialed in better meaning it wasn't too wet. Here a photo of how much side and bottom pins I got on this one. When fruiting an uncased tub next time I will try and restrict FAE quite a bit. There must be a sweet spot for getting a pin set on an uncased sub surface.

Wild Coast seems to be hardy. Those caps suffered from FAE burn but didn't abort when misted. Looks like the skin got blown right off their face.

Nec-D and Bunnell clones seem to have worked out although the donor fruits where spindly as. Looking forward to getting into result worthy testing with these.

Wild Coast spawned to straw/compost/leached sawdust colonized fine but has now a couple of bacterial spots.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 2
#27983017 - 10/05/22 03:36 PM (1 year, 3 months ago) |
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Amazing experiment! Don't think I've seen so many side/bottom pins with Pans yet 
That's absolutely nuts that they didn't abort. The wild coast really seem.. wild
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 4
#27983045 - 10/05/22 03:51 PM (1 year, 3 months ago) |
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Had this happening with Cambo Thai a few times, can't remember what I was trying back then.

I think this happens when blasting with FAE too much or at the wrong time. It rises O2 levels but lowers humidity at the surface. Just a big fat old theory but since substrates have no heads or tails breathing can happen on one end while pinning will be diverted to more moist areas/condition. Trying to get my focus down for when the clones are ready and then will try to explore only a couple of aspects in depth.
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Winston Smith
Little Brother


Registered: 09/03/22
Posts: 200
Loc: Over there!
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27983614 - 10/05/22 08:53 PM (1 year, 3 months ago) |
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Quote:
Baba Yaga said: Had this happening with Cambo Thai a few times, can't remember what I was trying back then.

I think this happens when blasting with FAE too much or at the wrong time. It rises O2 levels but lowers humidity at the surface. Just a big fat old theory but since substrates have no heads or tails breathing can happen on one end while pinning will be diverted to more moist areas/condition. Trying to get my focus down for when the clones are ready and then will try to explore only a couple of aspects in depth.

In this situation, I assume that the surface had few pins, is that right?
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Winston Smith
Little Brother


Registered: 09/03/22
Posts: 200
Loc: Over there!
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If anyone could share their opinion on the health of this plate, it'd be mighty righteous!
It's a Pan. cyan, T2 with the OG plate being a germinated swab.

Does the myc look right? This is my first go-round with the beautiful cyans and I'm still developing the eye for identifying it's unique characteristics.
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MacMerdin
Hunter



Registered: 04/26/12
Posts: 2,005
Loc: The Island of Misfit Toys
Last seen: 3 months, 17 days
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Re: Official Copelandia/Panaeolus Thread [Re: Winston Smith] 2
#27983772 - 10/05/22 10:17 PM (1 year, 3 months ago) |
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Looks good to me
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Winston Smith
Little Brother


Registered: 09/03/22
Posts: 200
Loc: Over there!
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Thank you! Stoked to hear that.
By the way, the recipe in your Pan-Cake Tek, does it scale up well? Store bought manure may be my only option, unfortunately. I've never grown these before, with quality/reliable manure source being my main deterrent. But if I could get some grains going with this plate and then s2b using that recipe in your tek, it would be a breeze. So would that be possible?
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MacMerdin
Hunter



Registered: 04/26/12
Posts: 2,005
Loc: The Island of Misfit Toys
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Re: Official Copelandia/Panaeolus Thread [Re: Winston Smith] 4
#27983822 - 10/05/22 10:50 PM (1 year, 3 months ago) |
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I used store bought manure so I think you should be fine as long as you keep everything sterile.
You can always make more than one slurry mix syringe with that plate as well. Let me know if you need directions to produce the syringes.
With multiple syringes you can expirement further.
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Winston Smith]
#27983839 - 10/05/22 11:08 PM (1 year, 3 months ago) |
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Quote:
Winston Smith said:
Quote:
Baba Yaga said: Had this happening with Cambo Thai a few times, can't remember what I was trying back then.

I think this happens when blasting with FAE too much or at the wrong time. It rises O2 levels but lowers humidity at the surface. Just a big fat old theory but since substrates have no heads or tails breathing can happen on one end while pinning will be diverted to more moist areas/condition. Trying to get my focus down for when the clones are ready and then will try to explore only a couple of aspects in depth.

In this situation, I assume that the surface had few pins, is that right?
Ha yeah, the photo below is the a shot of the surface and it looks like it was one of my first uncased experiments.
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Winston Smith
Little Brother


Registered: 09/03/22
Posts: 200
Loc: Over there!
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Re: Official Copelandia/Panaeolus Thread [Re: MacMerdin]
#27983856 - 10/05/22 11:29 PM (1 year, 3 months ago) |
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Quote:
MacMerdin said: I used store bought manure so I think you should be fine as long as you keep everything sterile.
You can always make more than one slurry mix syringe with that plate as well. Let me know if you need directions to produce the syringes.
With multiple syringes you can expirement further.
That's a relief, thank you.
You've got me seriously considering the slurry idea. I'm not too familiar with the concept, but I'm sure I could search my way around. One quick question about them, though. Is the entire plate used in the slurry-making process? I only ask because, if you look at the transfer in the pic I posted, there's some slight discoloration around the center. That'd be bacteria, yeah? If so, I'd want to avoid adding that part to a slurry, right?
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Winston Smith
Little Brother


Registered: 09/03/22
Posts: 200
Loc: Over there!
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27983858 - 10/05/22 11:31 PM (1 year, 3 months ago) |
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Quote:
Baba Yaga said:
Quote:
Winston Smith said:
Quote:
Baba Yaga said: Had this happening with Cambo Thai a few times, can't remember what I was trying back then.

I think this happens when blasting with FAE too much or at the wrong time. It rises O2 levels but lowers humidity at the surface. Just a big fat old theory but since substrates have no heads or tails breathing can happen on one end while pinning will be diverted to more moist areas/condition. Trying to get my focus down for when the clones are ready and then will try to explore only a couple of aspects in depth.

In this situation, I assume that the surface had few pins, is that right?
Ha yeah, the photo below is the a shot of the surface and it looks like it was one of my first uncased experiments.

Haha at least there was something up there. Keep in mind that success is relative. When my first Pan harvest comes in, I hope I'm able to get at least that! Haha
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MacMerdin
Hunter



Registered: 04/26/12
Posts: 2,005
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Re: Official Copelandia/Panaeolus Thread [Re: Winston Smith] 2
#27985047 - 10/06/22 05:40 PM (1 year, 3 months ago) |
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Quote:
Winston Smith said: Is the entire plate used in the slurry-making process? I only ask because, if you look at the transfer in the pic I posted, there's some slight discoloration around the center. That'd be bacteria, yeah? If so, I'd want to avoid adding that part to a slurry, right?
Yes, the entire plate is "used" as in the water will definitely touch everything on the plate. But you won't use up all the mycelium. So you want a clean plate to start with. If you are unsure with this plate, just make more. The more the merrier.
To make a syringe...start with a full sterile syringe of water. Heat the tip as usual. In a SAB, under a hood, or whatever you use to keep things clean open the plate slightly (I try not to open it as to let anything fall into it) and squirt the water onto the plate. Now is the tricky part as you have to swish the tip of the needle around to get some mycelium and agar and then suck it back into the syringe. It may look like you didn't get any but you did. The agar will dissolve giving the water nutrients for the mycelium to grow out in the syringe. Now you have a liquid culture without having to make a LC jar.
I usually limit my plates to 3 syringes. I just feel the more it's opened and the more it's touched the more odds of contamination.
Edited by MacMerdin (10/06/22 06:33 PM)
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Scotay
The Mad Mush Guy

Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 13 hours
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Re: Official Copelandia/Panaeolus Thread [Re: MacMerdin]
#27985388 - 10/06/22 07:59 PM (1 year, 3 months ago) |
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Damn Karri0n those B's look great!! Very well done Growmie!! I'm interested to see someone do pans without poop. Should be interesting!! It's just so cool to get opinions and examples from people's ups and downs!! Thanks for the info friends and neighbors!! Happy Thursday!! 😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] 1
#27985395 - 10/06/22 08:04 PM (1 year, 3 months ago) |
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Many have, and are currently growing pans without poop.
Personally, I love collecting, aging, and using horse poop.
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MycoBrainz
Mycotic



Registered: 03/14/15
Posts: 615
Loc: West Coast
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 2
#27985559 - 10/06/22 09:24 PM (1 year, 3 months ago) |
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I too Poop
--------------------
PFC x Creeper Lets Get Stoned
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
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Re: Official Copelandia/Panaeolus Thread [Re: MycoBrainz] 2
#27985876 - 10/07/22 05:57 AM (1 year, 3 months ago) |
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Pan ES 16 days from spawn in an EZ Dial uncased experiment a la Baba Yaga. Not sure what’s going on but surface is looking a little stressed fruiting sporadically though so I guess I’ll see what happens! Gunna case my next lot for sure!
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley]
#27986097 - 10/07/22 09:24 AM (1 year, 3 months ago) |
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I hope those fruits are going to mature since some of the caps are looking like they are damaged. Interesting though that the pins are only emerging from the areas with that dense overlay. I have one more uncased tub going at the moment and trying a different, more restricted hole setup with it. After that it's going back to casing for me as well. Will do more trials later.
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27986161 - 10/07/22 10:10 AM (1 year, 3 months ago) |
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Quote:
Baba Yaga said: I hope those fruits are going to mature since some of the caps are looking like they are damaged. Interesting though that the pins are only emerging from the areas with that dense overlay. I have one more uncased tub going at the moment and trying a different, more restricted hole setup with it. After that it's going back to casing for me as well. Will do more trials later.
Glad you chimed in bud. Yeah I’m of the same mindset, I think pans WILL fruit in a mono without a casing layer but the conditions are far from ideal.
In monotubs WITH a casing layer is a different story as you have proven with a plethora of evidence. I have a tub of Pan Jambo’s just about ready for casing and some casing that I’ve freshly pasteurised so will provide updates for that as and when both here and in Baba’s mono tub thread.
For those of you who haven’t had the chance to view this marvellous experiment then please head Here to view, contribute or theorise!
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,207
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 1
#27986361 - 10/07/22 12:39 PM (1 year, 3 months ago) |
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Are 'Hunter Valley' Australia cyans or tropicalis?
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 5
#27986491 - 10/07/22 02:09 PM (1 year, 3 months ago) |
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I've promised you guys something. HERE it is. The Bisporus MS flush in my new FC wasn't a fluke at all. This outdoor clone is loving the conditions as well (see the mountainous surface, Yoshi?)
I'll be updating my journal entry about easy AF Bisporus growing. As low-tek as it gets. No moving parts. Cased it and placed it into the FC, that's all I did until harvest (after that I misted the immature ones).
8cm tall Bisporus is not bad but I still feel they're tiny, compared to Cyans 😅 Which will also be fruited this way sometime soon.
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