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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 9,288
Loc: South Africa
Last seen: 4 hours, 36 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] 1
#27980567 - 10/04/22 02:50 AM (1 year, 3 months ago) |
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Well you uploaded a pdf so technically you still haven't uploaded a picture.
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay]
#27980595 - 10/04/22 04:12 AM (1 year, 3 months ago) |
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I'm not clicking that, and I don't see any pictures.
Edited by ruawakeyet (10/04/22 04:13 AM)
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0t0lerance


Registered: 05/01/21
Posts: 962
Loc: The Netherlands
Last seen: 4 days, 12 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay]
#27980613 - 10/04/22 04:45 AM (1 year, 3 months ago) |
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Quote:
Scotay said: Test picture
Google "how to upload pic shroomery" https://www.shroomery.org/forums/showflat.php/Number/27155585#27155585
Here you go, good luck.. I'm actually surprised how many threads there are about uploading a picture
Edited by 0t0lerance (10/04/22 04:47 AM)
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 36 minutes, 46 seconds
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Re: Official Copelandia/Panaeolus Thread [Re: 0t0lerance] 4
#27981086 - 10/04/22 11:53 AM (1 year, 3 months ago) |
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Sketchy ass mycelium. The t1 seems to be looking normal though.
--------------------
LAGM2020     
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 3 hours, 36 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a] 1
#27981134 - 10/04/22 12:35 PM (1 year, 3 months ago) |
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It happens. When I put my Cyans on the new experimental agar, it grew funky as fuck too. However I'm sure the culture is fine, just doing what Pans do sometimes.
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,209
Loc: Castles made of sand
Last seen: 48 minutes, 46 seconds
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27981380 - 10/04/22 03:09 PM (1 year, 3 months ago) |
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How necessary is it to have a perfectly flat substrate? I run my bulk in bags and preform them to fit in shoeboxes. I manage to get the surface fairly flat but not like you guys with the hand tools.
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Scotay
The Mad Mush Guy

Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 18 hours
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#27981427 - 10/04/22 03:26 PM (1 year, 3 months ago) |
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Any advantage or disadvantages using diaotmaceous earth product as a preventative measure? This has been a really crazy years for bugs and I don't wanna fuck this up. Cleanliness is paramount from the go here. Thanks in advance for your help friends and neighbors!! Happy Tuesday!! 😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 1
#27981434 - 10/04/22 03:31 PM (1 year, 3 months ago) |
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Yoshi, I never used anything other than a tablespoon, but I am planning on getting one of those grout trowels. I am hoping to save some time on the final leveling by using a trowel.
I don't think it is crucially important to have it perfectly flat. I think the goal is to avoid any significant high or low spots so that you don't have high and dry(er) spots, nor low spots that might pool with water.
Scotay, I don't know that DE would do much good in mycology, as the fruiting conditions are too wet to leave the DE as a powder. Once it gets wet, bugs can more easily avoid it.
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karri0n
Mind Traveller



Registered: 08/29/20
Posts: 698
Last seen: 16 days, 5 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 8
#27981534 - 10/04/22 04:23 PM (1 year, 3 months ago) |
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Here's my latest bisporus grow.
I prepared 2.5 quarts straw, 2.5 quarts Scott's manure/hummus, 1 quart verm, brought this to field capacity, and sealed it in a myco-bag.
I pasteurized this in a crock pot on warm for ehh 16 hours or so. The highest temp anywhere I could find with the digital meat thermometer probe goes to 165 with this method in my crock pot, but I'd always recommend thoroughly testing yours.
I let this sit on a shelf for five days.
I flamed sterilized some scissors, and in my SAB, cut open the bag, then inoculated the substrate with 1 quart of 50/50 oats/wbs spawn fully colonized with panaeolus bisporus.
I re-sealed the bag, still in my SAB, then lifted the SAB off and thoroughly mixed the grain throughout the sub.
I let this colonize, which took about 7 to 10 days, I don't recall.
I then laid this to two different trays - one shoebox, and one shorty tray(originally from some food packaging) to do a side by side in the JCM chamber.
I put foil over the shorty tray and the regular shoebox lid over the shoebox, and allowed this to colonize for 10 days.
After 10 days, I removed the covers and cased using jiffy mix brought to field capacity and pasteurized in a crockpot for 12 hours. I thoroughly misted the casing with distilled water.
I placed them into the JCM chamber which I have regulated to 75F using a temperature controller. This is a slight modification to the JCM design but allows me to fiddle with it less, to account for changes in room temp, and to get less, or more, water raining down onto the substrate from the lid.
On cooler days I've had this as low as 72, and on days the dehydrator is running in the lab, the ambient temp goes to 77, so the JCM gets set to 79. Generally, a 2 to 4 degree delta from ambient room temp seems ideal in my setup.
After about 15 days, I got the first flush. The shorty tray made smaller mushrooms and less weight, and just seemed less healthy overall with less vigorous mycelium.

Overall, the shoebox with the deeper substrate performed better than the shorty tray. I use twist and pull to harvest - sliding a knife over the edge of the tray just has never worked as cleanly or easily as I want - so the short sides don't provide me any real advantage other than these sweet side view shots:

From the two trays, I got 24g dried for the first flush. Not bad for pans and 1 quart of spawn.

On the second flush, you can really see how the shorty tray has run out of steam while the shoebox with the taller sub has some fight in it still:

On my next run, I will do three shoeboxes in this chamber.
It should be noted that this is the exact same culture that produced the 22g monster bisporus in my signature, and these bisporus are possibly *smaller* than average.
This proves to me even further that the previous result was due to environmental conditions, namely, less water in the casing and possibly less water in the substrate overall, at pinning.
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Panaeolus Bisporus
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 5 hours
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Re: Official Copelandia/Panaeolus Thread [Re: karri0n] 5
#27981536 - 10/04/22 04:25 PM (1 year, 3 months ago) |
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Cloned my first pan, ive had 8 fruits in a passive dubtub
i am now a pan grower in the school of baga yaga,
treating them as cubes, no manure, no mercy
hail satan pans also belong to me now
-------------------- Willpower is the one true virtue
  
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: karri0n] 1
#27981585 - 10/04/22 05:06 PM (1 year, 3 months ago) |
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karri0n, holy cow those are some beauties! I love seeing so many people pulling down SOLID flushes of bisporus. Not to mention that monster in your sig. That's the heaviest pan I've seen on here, and it was really well formed for being a giant!
smalltalk, congratulations! I think you'll really like fruiting your clone culture! I wouldn't mind some pictures, though...
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: karri0n] 1
#27981793 - 10/04/22 07:33 PM (1 year, 3 months ago) |
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Quote:
karri0n said: This proves to me even further that the previous result was due to environmental conditions, namely, less water in the casing and possibly less water in the substrate overall, at pinning.
Interesting you are saying this, I think that the mycelium colonizes the substrate much better when having it more on the dryer side which in return makes for a better infrastructure for water and nutrient transport later on. Still soaking my casings though but one can't argue with results.
Nonetheless great stuff karri0n, bisporus is just the most beautiful pan. It's those wavy caps which are giving me the butterflies.
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Quote:
smalltalk_canceled said: Cloned my first pan, ive had 8 fruits in a passive dubtub
i am now a pan grower in the school of baga yaga,
treating them as cubes, no manure, no mercy
hail satan pans also belong to me now
Make sure to post updates and photos when running no manure. Me wants to see.
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Scotay
The Mad Mush Guy

Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 18 hours
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Re: Official Copelandia/Panaeolus Thread [Re: DERRAYLD]
#27982121 - 10/04/22 10:52 PM (1 year, 3 months ago) |
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Quote:
DERRAYLD said: Well you uploaded a pdf so technically you still haven't uploaded a picture.
I am realizing that!!!! Shit!!!! Trial and error! Happy Tuesday/Wednesday!! 😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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Scotay
The Mad Mush Guy

Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 18 hours
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#27982128 - 10/04/22 10:58 PM (1 year, 3 months ago) |
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Quote:
ruawakeyet said: Yoshi, I never used anything other than a tablespoon, but I am planning on getting one of those grout trowels. I am hoping to save some time on the final leveling by using a trowel.
I don't think it is crucially important to have it perfectly flat. I think the goal is to avoid any significant high or low spots so that you don't have high and dry(er) spots, nor low spots that might pool with water.
Scotay, I don't know that DE would do much good in mycology, as the fruiting conditions are too wet to leave the DE as a powder. Once it gets wet, bugs can more easily avoid it.
Thanks so much for the heads up my friend! I appreciate that! Best not to get bugs in the first place. I only had an issue once with cube tub. Little crawly bastards got in and boom had to toss the tub. Really sucked because even though they were cubes they were looking good. Appreciate you taking the time to answer!! Peace and chicken grease!! 😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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Art_Vandelay.93


Registered: 08/13/22
Posts: 20
Loc: Michigan, USA
Last seen: 1 year, 3 months
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] 9
#27982244 - 10/05/22 03:40 AM (1 year, 3 months ago) |
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My beautiful Panaeolus Cyanescens! (Estero)✌️❤️🍄👽🛸



My only concern is that the mycelium ate through the aluminum “pans” and absorbed hydrogen peroxide water from underneath(heating tub underneath pans). Not only do I think I have extreme potency loss, I think they have a bitter taste? I’m not sure if Pan Cyans are supposed to taste this way but I know for sure the 6 grams I ate yesterday (throughout the day) should have made me at least see visuals. These are legit “Estero” genetics directly from GordoTEK. Him and I have been working together on this particular strain/variety. I have also told him my findings this morning , but am waiting for his input. I truly am curious to hear what you all think about this situation. Either way, I have more pans colonizing as we speak and am gonna drain the water from the tub to be safe either way. I plan on using plastic trays next time of course. Wish I would have this time. Anyways, any input is greatly appreciated and mush love ✌️❤️🍄👽
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 36 minutes, 46 seconds
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Those are insane man. Like pans with PE caps.
Cubes will eat aluminum also, I think that’s why people switched to plastic tubs.
What do you guys think about this, I’m a little rusty on my pan skills.
I’m liking the speed of the top colony, but kinda want to take the thicker side of the bottom one also. It looks more like what I used to grab when I grew pans but it was mostly jambo.
--------------------
LAGM2020     
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a] 3
#27982522 - 10/05/22 09:30 AM (1 year, 3 months ago) |
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Quote:
karri0n said: Here's my latest bisporus grow.
I prepared 2.5 quarts straw, 2.5 quarts Scott's manure/hummus, 1 quart verm, brought this to field capacity, and sealed it in a myco-bag.
I pasteurized this in a crock pot on warm for ehh 16 hours or so. The highest temp anywhere I could find with the digital meat thermometer probe goes to 165 with this method in my crock pot, but I'd always recommend thoroughly testing yours.
I let this sit on a shelf for five days.
I flamed sterilized some scissors, and in my SAB, cut open the bag, then inoculated the substrate with 1 quart of 50/50 oats/wbs spawn fully colonized with panaeolus bisporus.
I re-sealed the bag, still in my SAB, then lifted the SAB off and thoroughly mixed the grain throughout the sub.
I let this colonize, which took about 7 to 10 days, I don't recall.
I then laid this to two different trays - one shoebox, and one shorty tray(originally from some food packaging) to do a side by side in the JCM chamber.
I put foil over the shorty tray and the regular shoebox lid over the shoebox, and allowed this to colonize for 10 days.
After 10 days, I removed the covers and cased using jiffy mix brought to field capacity and pasteurized in a crockpot for 12 hours. I thoroughly misted the casing with distilled water.
I placed them into the JCM chamber which I have regulated to 75F using a temperature controller. This is a slight modification to the JCM design but allows me to fiddle with it less, to account for changes in room temp, and to get less, or more, water raining down onto the substrate from the lid.
On cooler days I've had this as low as 72, and on days the dehydrator is running in the lab, the ambient temp goes to 77, so the JCM gets set to 79. Generally, a 2 to 4 degree delta from ambient room temp seems ideal in my setup.
After about 15 days, I got the first flush. The shorty tray made smaller mushrooms and less weight, and just seemed less healthy overall with less vigorous mycelium.

Overall, the shoebox with the deeper substrate performed better than the shorty tray. I use twist and pull to harvest - sliding a knife over the edge of the tray just has never worked as cleanly or easily as I want - so the short sides don't provide me any real advantage other than these sweet side view shots:

From the two trays, I got 24g dried for the first flush. Not bad for pans and 1 quart of spawn.

On the second flush, you can really see how the shorty tray has run out of steam while the shoebox with the taller sub has some fight in it still:

On my next run, I will do three shoeboxes in this chamber.
It should be noted that this is the exact same culture that produced the 22g monster bisporus in my signature, and these bisporus are possibly *smaller* than average.
This proves to me even further that the previous result was due to environmental conditions, namely, less water in the casing and possibly less water in the substrate overall, at pinning.
Nice work! Those are excellent results. I love that you found a commercial manure product that produces good results because it will enable a lot of people who only have big box stores as an option to get manure.
Quote:
A.k.a said:

I like both of those - I agree with you and would take a transfer from the top culture, and also 9:00 on the bottom culture.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 3 hours, 36 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 4
#27982594 - 10/05/22 10:15 AM (1 year, 3 months ago) |
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Amazing work, Karrion and Vanderlay! Please note that mushrooms will absorb heavy metals from the environment. Like this you could be exposing yourself to unhealthy amounts of Aluminium, buddy. Pans are naturally bitter because they are so potent, but I'm sure the aluminium is not good for ya. 
Yoshi, Pans don't give a damn about flat substrate. If you saw the tub I have in fruiting now it would drive you crazy even if you have no OCD Pics coming soon, when they mature a little bit. I have them fruiting in the same FC with Subtrops. It fills me with immense joy having these two super rare exotics jsut casually growing in a tub side by side.
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,209
Loc: Castles made of sand
Last seen: 48 minutes, 46 seconds
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#27982727 - 10/05/22 12:16 PM (1 year, 3 months ago) |
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Quote:
Adas said: Yoshi, Pans don't give a damn about flat substrate. If you saw the tub I have in fruiting now it would drive you crazy even if you have no OCD Pics coming soon, when they mature a little bit. I have them fruiting in the same FC with Subtrops. It fills me with immense joy having these two super rare exotics jsut casually growing in a tub side by side.
Thank you Adas, i look forward to your pics. I'm always looking to improve my grows. In pics and reading, some very good pan growers are meticulous with flat/even subs. The thought occurred to me that the bottom of my bulk bags that are pressed against the shoebox are completely flat and smooth. I might flip a few subs over and give the OCD life a try. 
Edit: Thank you Ruawake for your response too!
Edited by YoshiTrainer (10/05/22 03:29 PM)
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