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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss]
    #27889244 - 08/04/22 03:53 AM (1 year, 5 months ago)

Quote:

Gorguss said:
YEa people say pans are not that much harder than cubes but i  feel like im chasing bigfoot here. I am struggling. I cant even read the myc in any meaningful way. Anyone have a link to a collage of pan myc and what it should and shouldnt look like




With the first few transfers from multispore I still find it difficult as well to see whats going on. In my experience it becomes clearer once you are taking clones.
Lowering the nutrient content can help as well. I'm currently on 1% but start thinking about going down to 0.5% for plates.


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InvisibleWall.E
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss]
    #27889310 - 08/04/22 06:29 AM (1 year, 5 months ago)

Quote:

Gorguss said:
YEa people say pans are not that much harder than cubes but i  feel like im chasing bigfoot here. I am struggling. I cant even read the myc in any meaningful way. Anyone have a link to a collage of pan myc and what it should and shouldnt look like




Jcm has a few pics


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: myco.bay]
    #27889364 - 08/04/22 07:27 AM (1 year, 5 months ago)

Quote:

myco.bay said:
I really need to find a better way to pasteurize my substrate. I've been using an instantpot but I don't think it's doing the trick. My pan myc grows out a bit from the spawn but then seems like it's stalling every time. Interestingly my subtropicalis grows just fine in the same substrate.




I loved the idea of the Instapot and used it to successfully grow pans for over a year but results were more hit-and-miss than I would have liked. I would often get seedlings growing in my substrate during colonization and with proper pasteurization that should never happen. So I built a steam sterilizer. So far I'm having much more consistent success with my trays - lower contamination rates and higher success rates. I still get trich here and there as we all do, but it's even less common now. You can see the steam pasteurizer setup in my journal. Very easy and cheap to build. Alternative, you can use the tried-and-true stovetop method that Gorguss mentioned though there are different variations and I would advise going to 160F internal.


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InvisibleTheDoobskerS
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 3
    #27889667 - 08/04/22 11:37 AM (1 year, 5 months ago)

Put these 4 T2s to grain yesterday

I think they look a little more fluffy than desired, but my eyes are not very trained for pans :shrug:

It also may have to do with my agar recipe which includes nutritional yeast



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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: TheDoobsker] * 1
    #27889672 - 08/04/22 11:44 AM (1 year, 5 months ago)

Those look great. They have good organization lines moving outward from the center. Yes a bit fluffy and very dense - but as you said, that's likely due to your agar recipe. I quit using peptones, yeast, and LME and went to just straight GWA and find much more consistent results with mycelium that is better defined and easier to see what's going on.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Imatryin]
    #27889734 - 08/04/22 12:35 PM (1 year, 5 months ago)

Quote:

Imatryin said:
Thanks for sharing your experience.  I have a 6 jars. I will leave some out and refrigerate others. Update in about a month.
And I will keep my thumbs off the thread titles πŸ‘





Have a look at this freshly posted thread where Ryuu has spawned pan grain after 4 weeks in the fridge.

https://www.shroomery.org/forums/showflat.php/Number/27889429/gonew/1#UNREAD


Edited by Baba Yaga (08/04/22 03:13 PM)


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InvisibleTheDoobskerS
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27889737 - 08/04/22 12:38 PM (1 year, 5 months ago)

Quote:

Hindsight said:
Those look great.



:fuckyeah:

Quote:

Hindsight said:
I quit using peptones, yeast, and LME and went to just straight GWA and find much more consistent results with mycelium that is better defined and easier to see what's going on.



Cool good to know! I prefer no yeast in my agar for pans, but I like the results I see with cubes. Out of laziness, I don't pour two separate recipes. GWA sounds nice and easy I think I'll give that a try in the future


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: TheDoobsker] * 1
    #27889778 - 08/04/22 01:10 PM (1 year, 5 months ago)

I do 700ml of distilled water plus 300ml of grain water (the water used for my grain prep - should be fairly dark).


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InvisibleTheDoobskerS
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27889817 - 08/04/22 01:35 PM (1 year, 5 months ago)

Awesome thanks Hindsight :thumbup:

I'm definitely gonna give this a try


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InvisibleHILLBILLY OUTLAWS
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss]
    #27889820 - 08/04/22 01:38 PM (1 year, 5 months ago)

Quote:

Gorguss said:
Quote:

myco.bay said:
I really need to find a better way to pasteurize my substrate. I've been using an instantpot but I don't think it's doing the trick. My pan myc grows out a bit from the spawn but then seems like it's stalling every time. Interestingly my subtropicalis grows just fine in the same substrate.




Easiest way is to use large pots and a gas burner. Bring the temp of the water to 150Β°F and then find the sweet spot on the gas burner to keep it right there. after 3 hrs you should be good to go. dump or pull the bags and let cool for 24hrs before use.



This is how I pasteurize my casing mixes.


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Offlinemickeydee
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Re: Official Copelandia/Panaeolus Thread [Re: HILLBILLY OUTLAW] * 4
    #27891894 - 08/05/22 09:58 PM (1 year, 5 months ago)

Alright Shroomery wizards, every time I master a new skill, I end up needing more tools for solving the puzzle. Here are some gorgeous new finds from the wild.

The beautiful mushroom giving rainclouds.

Now instead of excitedly eating them, I took spore prints this time. Since they are wild spore prints, I knew they would be contaminated but I gave them a shot anyways, and now all my plates have turned out very bacterial. The contam is certainly from the spores as my unused plates have no growth and my inoculation tool was always flame sterilized before use. Is it still possible i could get a transfer from these plates?

Other options I have read about: 1. Streaking the spores in a Z to create more spread of spores and contam 2. Making diy antibacterial agar 3. Mixing the spores with water and using a very small droplet to put use as few spores and contam as possible 4. Try to get the smallest speck of spores to agar instead of dropping larger visible pieces on the plate 5. Dont even bother and use a professional print with way less contam.

Any experience on what would work? I'd love to cultivate from my own wild finds.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: mickeydee]
    #27892083 - 08/06/22 04:13 AM (1 year, 5 months ago)

Those are beauties indeed.

I would let the plates sit for a while, sometimes mycelium can grow out of the mess but the
alternatives you have mentioned could work as well. Streaking in a zigzag pattern an diluting
spores are good ways. The only antibacterial agar I used is tea agar which doesn't work for
germinating spores though. Never used antibiotic agar.

A good method imo is to germinate on small BRF/verm pucks like Josex describes in this thread

https://www.shroomery.org/forums/showflat.php/Number/24806569#24806569

it is working really well to get rid of bacteria.


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OnlineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #27892464 - 08/06/22 11:50 AM (1 year, 5 months ago)

I haven't really had bacterial problems with any spore prints, which tells me it must be your technique. However, sometimes it helps to let the spore prints sit for a while before using them. A lot of bacteria won't survive that.


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Invisiblekirkeng
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 6
    #27892482 - 08/06/22 12:05 PM (1 year, 5 months ago)

Been working some more bisporus in unmodified, first pins littered the sides so initially I was bumming, but the top layers have been slowly filling out nicely, not as even as I’d like but I’m gunna take what I can get. Fingers crossed I can get some good flushes out of these guys. Cloned culture from the first grow I did recently, already seeing some improvement. This culture hates grain though so I’ve completely accepted using sterile manure spawn which has been working much better. Generally I use field dried nuggets but the folks I got it from have started refusing me so got a new person but there’s a lot more wood shavings and some composted mixed in, glad it’s not having any I’ll effects. Also recently found a cintulus nearby my home, no pics, but got it printed and sending to agar soon. Cambos on grain excited to see how those compare to bisporus once they’re ready. Gotta say though bisporus has been real good to me lately.



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OnlineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
    #27892486 - 08/06/22 12:07 PM (1 year, 5 months ago)

Hell yeah man, another amazing Bisporus grow! Congrats!


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Invisiblekirkeng
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 2
    #27892491 - 08/06/22 12:12 PM (1 year, 5 months ago)

Thanks man, forgot to add, in the first grow I had my window open the whole time, this time no open window or fan going in the room. Just passive air from the rather stagnant room. So seems the air exchange requirements are less than I previously thought. Think I’m going to keep trying to restrict that in the future and see how far I can push it this winter.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
    #27892622 - 08/06/22 02:44 PM (1 year, 5 months ago)

Cool kirkeng, those tubs are looking great.

will be interesting to see how the clone is going to perform.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27892633 - 08/06/22 02:58 PM (1 year, 5 months ago)

Quote:

Adas said:
I haven't really had bacterial problems with any spore prints, which tells me it must be your technique. However, sometimes it helps to let the spore prints sit for a while before using them. A lot of bacteria won't survive that.





Good point, technique might be the culprit here.


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OfflineYoshiTrainer
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 6
    #27893087 - 08/06/22 10:47 PM (1 year, 5 months ago)

I wasn't going to let them go another flush but they made me an offer I could not refuse.



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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] * 7
    #27893150 - 08/07/22 12:41 AM (1 year, 5 months ago)

Nec D


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