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Invisiblecambocyan

Registered: 06/24/20
Posts: 74
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 2
    #27886774 - 08/02/22 11:14 AM (1 year, 5 months ago)

nice job hindsight care to share your timimg cycles for your martha tent you seem to have it dialed in especially with those bisporus also how much water do you go through a day.
I'm also using a gh using a humidity controller but want to switch over to timers.


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27886781 - 08/02/22 11:18 AM (1 year, 5 months ago)

Yea these days I also cover everything with a top layer just to be sure. It also ends up looking better which is a nice bonus. :lol:

Beautiful Cambos! Finally some Pan porn here.


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Offline0t0lerance
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Re: Official Copelandia/Panaeolus Thread [Re: cambocyan]
    #27886782 - 08/02/22 11:18 AM (1 year, 5 months ago)

still Estero prints available :pm: if you're interested.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: cambocyan] * 1
    #27886889 - 08/02/22 12:34 PM (1 year, 5 months ago)

Quote:

cambocyan said:
nice job hindsight care to share your timimg cycles for your martha tent you seem to have it dialed in especially with those bisporus also how much water do you go through a day.
I'm also using a gh using a humidity controller but want to switch over to timers.




I started with a humidity controller. The issue is that they are slow to react, and at the humidity levels we need to run to go beyond humidification and actually ADD water to the substrate, the sensors flood out and stop working or take way too long to dry out in order to actually measure the RH.

I will give you my cycles but they won't really mean much since the cycles required are highly variable based on so many things like size of greenhouse, what fogger you are using, the size of the reservoir the fogger is in and the level of the water, size and length of ducting, size and strength and speed of fan (both the intake and exhaust fan), RH of the room, and temp of the room.

My cycle is: 2 mins of fog, 1 min of venting, and 11 mins of pause.

I have never measured how much water I am consuming but I would guess it to be 3 gallons every 4-5 days.

My room RH is 50% using an automated room humidifier and my temp is set to 77F.


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Invisiblecambocyan

Registered: 06/24/20
Posts: 74
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27886935 - 08/02/22 01:29 PM (1 year, 5 months ago)

That's exactly the information I was looking for I'm sure my humidity sensor is going bad on me as I'm having trouble dialing my humidity in this year since I took a small break also watering can be a chore on a daily basis.

Mine is a two-tier with a small air pump with a single disc fogger.
My RH and temperature seems to be similar to yours as I live in a desert climate

ill keep dialing it in thanks for the response.


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InvisiblePoison Drink
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27887509 - 08/02/22 09:53 PM (1 year, 5 months ago)

Quote:

Hindsight said:
But, I noticed something that just confirms my years long fear of seeds in straw. My fear being that these are vectors for contamination. You effectively have uncolonized grain spawn in your trays sitting out in open air and in contact with things not sterilized.




The straw I use also has quite a few grains left in it, and it has given me similar problems in the past. The mycelium would not colonize the pasteurized leftover grains and it would eventually contaminate. Due to this, I always sterilize straw now, which is a bit unwieldy. I have also been thinking about using hemp stalks, but I did not give it a try yet either.


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InvisibleBaba Yaga
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Registered: 09/13/20
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Re: Official Copelandia/Panaeolus Thread [Re: Poison Drink] * 2
    #27887523 - 08/02/22 10:14 PM (1 year, 5 months ago)

I started having similar concerns when using pea straw for growing oysters cause I was finding whole peas in there.
This is just another reason not to use straw in the bulk substrate. I guess this problem could be reduced with an
additional processing step but straw is already a bit too labor intensive for my taste. Still got 3 bales in the
garage so I will have some around for a while using it mainly for oysters.

Sterilizing straw manure bottles as we speak but I'm using these to do some clone hunting. Sterilized
substrates have never worked out in mono tubs which is why I'm using coir. Once I have a few cultures
ready I will try leached sawdust in my bulk subs instead of coir (Adas uses saw dust)...would be great if
this works out cause saw dust is available, cheap and fairly easy to prep.


Congrats on your steamer build Hindsight, sounds like its a winner and thanks for the update via PM. Those Cambos
are looking very happy.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #27887762 - 08/03/22 08:04 AM (1 year, 5 months ago)

Quote:

Baba Yaga said:
I started having similar concerns when using pea straw for growing oysters cause I was finding whole peas in there.
This is just another reason not to use straw in the bulk substrate. I guess this problem could be reduced with an
additional processing step but straw is already a bit too labor intensive for my taste. Still got 3 bales in the
garage so I will have some around for a while using it mainly for oysters.

Sterilizing straw manure bottles as we speak but I'm using these to do some clone hunting. Sterilized
substrates have never worked out in mono tubs which is why I'm using coir. Once I have a few cultures
ready I will try leached sawdust in my bulk subs instead of coir (Adas uses saw dust)...would be great if
this works out cause saw dust is available, cheap and fairly easy to prep.


Congrats on your steamer build Hindsight, sounds like its a winner and thanks for the update via PM. Those Cambos
are looking very happy.




Agree on extra straw processing time. I like the sawdust idea but seems like it would take extra prep time as well since you have to leach it. Has Adas given details on how he leaches it and for how long? I might give that a shot. I have to leach my horse manure anyway and it's a pretty passive process but takes months. I am hoping the shredded hemp is the answer because it is easy to get, not that expensive (not nearly as cheap as straw but how many of us are growing massive bulk grows of pans anyway) and requires no processing or work to use. Again, I have had good success with the hemp but also a few failures and I just need more time to test it.


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InvisibleImatryin
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Registered: 07/06/21
Posts: 412
Refrigerating spawn? [Re: Fantastic Mr. Fox]
    #27887829 - 08/03/22 08:43 AM (1 year, 5 months ago)

I see from posts that pan cyans can be refrigerated as LC. My current grain jars were inoculated with agar from once-refrigerated plates. I brought those plates back to room temp for 3 d, thinking it would give the myc enough time to start growing again. The questions: Will 100% colonized grain chill okay for 3 weeks?  The jars are ready but in 9 days I will be gone for 2 weeks. Also, each jar has a single 5/16” lid hole with 3 layers of Micropore tape. Should I close the holes more tightly to reduce drying out?
Thank you in advance for your help. This could be my first pan harvest!


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OfflineAdas
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Re: Refrigerating spawn? [Re: Imatryin] * 2
    #27888235 - 08/03/22 01:49 PM (1 year, 5 months ago)

I leech the sawdust by putting the bricks into a bucket that has 5 small holes at the bottom. I top the bucket with water and let it drain. Repeat. The holes get plugged occasionally, but that's alright, just poke the holes at the bottom with a nail and it flows again. You also sometimes need to stir it around because the sawdust that's in the center doesn't get leeched otherwise and will cause mold problems. Then you just flip the bucket and let the wet sawdust "cake" slide out onto an old shirt or something. Leave it in the air to drip and dry out a bit.


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InvisibleBaba Yaga
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Re: Refrigerating spawn? [Re: Adas]
    #27888330 - 08/03/22 02:45 PM (1 year, 5 months ago)

Wait, you don't let it sit for a few days before changing the water?
How many times or are you judging by the color of the water when it's done?


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OfflineAdas
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Re: Refrigerating spawn? [Re: Baba Yaga] * 2
    #27888343 - 08/03/22 02:54 PM (1 year, 5 months ago)

I usually change the water once or twice per day. Sometimes more if I have the time for it. I think it's done when there's minimal amounts of foam when you refill the bucket. It's honestly not easy to tell, so better let it go longer than face mold issues down the line. Then again, if you're gonna use it immediately, incomplete leeching should not cause huge issues in most cases.


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Offlineacapulcogold
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Re: Refrigerating spawn? [Re: Adas]
    #27888425 - 08/03/22 04:08 PM (1 year, 5 months ago)

Do you guys happen to have copelandia mycelium on agar pictures ?


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Imatryin] * 1
    #27888633 - 08/03/22 06:52 PM (1 year, 5 months ago)

Thanks Adas, will play around with this for a bit.


Quote:

acapulcogold said:
Do you guys happen to have copelandia mycelium on agar pictures ?




Not sure how recent taxonomy is distinguishing between copes and pans. They are just interchangeable names as far as I am
concerned. Therefore I would assume that copelandia mycelium does look more or less like panaeolus, surely not more different
than different varieties of panaeolus would compare to each other. I would also assume that if they are considered to be different
species then there will have a lot of mislabeling been going on. :shrug:

Any particular reason you are asking, got any photos of your plates?



Quote:

Imatryin said:
I see from posts that pan cyans can be refrigerated as LC. My current grain jars were inoculated with agar from once-refrigerated plates. I brought those plates back to room temp for 3 d, thinking it would give the myc enough time to start growing again. The questions: Will 100% colonized grain chill okay for 3 weeks?  The jars are ready but in 9 days I will be gone for 2 weeks. Also, each jar has a single 5/16” lid hole with 3 layers of Micropore tape. Should I close the holes more tightly to reduce drying out?
Thank you in advance for your help. This could be my first pan harvest!





All you can do is try, if your spawn is clean and your fruiting conditions are good then you should get something out of it.
I had spawn sitting on the shelf at room temp for a couple of weeks and it worked out alright.

Next time leave the thread title as is please.


Edited by Baba Yaga (08/03/22 07:05 PM)


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OfflineGorguss
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27888758 - 08/03/22 08:29 PM (1 year, 5 months ago)

Is it normal for Pan myc to bruise deep blue after taking wedges?


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Offlinethetryptkeeper
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Registered: 05/25/22
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss] * 6
    #27888826 - 08/03/22 09:13 PM (1 year, 5 months ago)



my first pan cyan pin :laugh: now hopefully more start popping up soon


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Spread the Love and Light as far as you can


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Offlinemyco.bay
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Re: Official Copelandia/Panaeolus Thread [Re: thetryptkeeper]
    #27888840 - 08/03/22 09:20 PM (1 year, 5 months ago)

I really need to find a better way to pasteurize my substrate. I've been using an instantpot but I don't think it's doing the trick. My pan myc grows out a bit from the spawn but then seems like it's stalling every time. Interestingly my subtropicalis grows just fine in the same substrate.


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OfflineGorguss
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Re: Official Copelandia/Panaeolus Thread [Re: myco.bay]
    #27888897 - 08/03/22 09:56 PM (1 year, 5 months ago)

YEa people say pans are not that much harder than cubes but i  feel like im chasing bigfoot here. I am struggling. I cant even read the myc in any meaningful way. Anyone have a link to a collage of pan myc and what it should and shouldnt look like


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OfflineGorguss
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Re: Official Copelandia/Panaeolus Thread [Re: myco.bay]
    #27888904 - 08/03/22 09:59 PM (1 year, 5 months ago)

Quote:

myco.bay said:
I really need to find a better way to pasteurize my substrate. I've been using an instantpot but I don't think it's doing the trick. My pan myc grows out a bit from the spawn but then seems like it's stalling every time. Interestingly my subtropicalis grows just fine in the same substrate.




Easiest way is to use large pots and a gas burner. Bring the temp of the water to 150°F and then find the sweet spot on the gas burner to keep it right there. after 3 hrs you should be good to go. dump or pull the bags and let cool for 24hrs before use.


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InvisibleImatryin
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27889079 - 08/04/22 12:15 AM (1 year, 5 months ago)

Thanks for sharing your experience.  I have a 6 jars. I will leave some out and refrigerate others. Update in about a month.
And I will keep my thumbs off the thread titles 👍


Edited by Imatryin (08/04/22 12:15 AM)


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