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Rad Aghast



Registered: 12/07/18
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Re: Official Copelandia/Panaeolus Thread [Re: SilverSumm]
#27882508 - 07/30/22 06:15 AM (1 year, 5 months ago) |
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Hey all - its been a while. Wondering what is the latest consensus on the best agar recipe for pans? I have typically used MEA but its been a while. thx.
RA
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MasterChipmunk
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
#27882765 - 07/30/22 11:06 AM (1 year, 5 months ago) |
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MEA should work just fine. That's what I use and I get plenty of quick growth.
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
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Re: Official Copelandia/Panaeolus Thread [Re: MasterChipmunk] 9
#27883111 - 07/30/22 04:27 PM (1 year, 5 months ago) |
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Recovering from my screw up, this is flush 1.5-2 of Ho'okipa.
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Tweeq
Tweeq of Nature


Registered: 06/07/18
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 1
#27883144 - 07/30/22 04:54 PM (1 year, 5 months ago) |
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Quote:
YoshiTrainer said: Recovering from my screw up, this is flush 1.5-2 of Ho'okipa.

Nice! In a mono?
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
#27883148 - 07/30/22 05:03 PM (1 year, 5 months ago) |
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Thank you Tweeq, it is a dub with an aquarium pump/bubbler set up.
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Gorguss
Chaotic sums


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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 1
#27883301 - 07/30/22 06:54 PM (1 year, 5 months ago) |
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any good teks for an aquarium pump/bubbler set up?
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss] 2
#27883564 - 07/30/22 10:16 PM (1 year, 5 months ago) |
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Not sure of a TEK but for two shoeboxes, I use an 150 gallon air pump that has a bubble stone attached to it. The bubble stone is in the bottom of a 1/2 gallon Mason jar that is 1/2 full of water with about 10% of that being hydrogen peroxide. From there it flows into a small mouth quart jar with 1/4" salt in the bottom. From that jar it splits into two tubes, one for each shoebox. At the shoebox, the tube splits again for two entry points into the shoebox. The hoses enter into the upper dub about mid wall, spaced evenly. Make sure the lids you are using for your Mason jars have silicon seals. When you drill the holes in the lids and tubs, drill them slightly small for tight seals.

This is what my dubs look like, the upper left and right hole would have the Polyfil replaced with hoses.
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THE MYCOSMITH
Weekend mycologist


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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 1
#27883653 - 07/31/22 12:25 AM (1 year, 5 months ago) |
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Hey Yoshi trainer Can you please explain whats the idea with the salt.?
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Nobler Hino
a dojo and a forge?!


Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
#27883729 - 07/31/22 06:12 AM (1 year, 5 months ago) |
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I love all the pan agar porn. Makes me want to swab some bama
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   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.β β Maria Sabina
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Rad Aghast



Registered: 12/07/18
Posts: 133
Loc: Minnesota
Last seen: 4 months, 29 days
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Quote:
MasterChipmunk said: MEA should work just fine. That's what I use and I get plenty of quick growth.
thx!
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] 1
#27883897 - 07/31/22 09:39 AM (1 year, 5 months ago) |
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Quote:
THE MYCOSMITH said: Hey Yoshi trainer Can you please explain whats the idea with the salt.?
The smaller jar removes some of the humidity and the salt in the bottom is to prevent mold in the jar or at least slow it.
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THE MYCOSMITH
Weekend mycologist


Registered: 01/24/22
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 2
#27883950 - 07/31/22 10:45 AM (1 year, 5 months ago) |
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Ok Yoshi trainer i see now a water drop collector with mold an bacteria kriller at the bottom. Cool.
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YoshiTrainer
Onion tied to belt



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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] 1
#27883979 - 07/31/22 11:20 AM (1 year, 5 months ago) |
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It sounds so much better when you put it that way, Mycosmith!
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mickeydee
Stranger


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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#27885157 - 08/01/22 09:48 AM (1 year, 5 months ago) |
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I am having trouble starting pan spores on agar. 12 plates and no growth at all after a week. Recipe used was 5g agar, 5g LME, and 250 mL distilled water per batch. I believe the only thing i grew was a lonely mold spot on one plate.
The print i got had been sitting for 8 months in my drawer room temperature. I wonder if its just not a viable print for some reason. I've already ordered another pan print from another source. Any advice on what to do different in the next attempt? Also including pic from my plate just to verify it is mold.
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Baba Yaga
β₯ coir grower

Registered: 09/13/20
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Re: Official Copelandia/Panaeolus Thread [Re: mickeydee]
#27885329 - 08/01/22 12:43 PM (1 year, 5 months ago) |
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Yes, this is mold unfortunately. I would wait for another 1-2 weeks before totally giving up on these plates. Your agar recipe is OK and an 8 month old print should germinate something good. Well, you got a new print on the way which might work better/faster.
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Vast218
Learning


Registered: 04/24/22
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27885393 - 08/01/22 01:39 PM (1 year, 5 months ago) |
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Ignoring the pieces I cut out, can anyone tell me if these look more like mold or mycelium? Iβm posting this here and not in the agar thread because Iβm dealing with spores of a wild panaeolus, so not only am I getting all sort of weird growth from the wild sample, but Iβm not sure anyone know what the mycelium is even supposed to look like lol
Itβs definitely panaeolus sp tho. Any help appreciated
1

2

3
Edited by Vast218 (08/02/22 05:37 AM)
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Baba Yaga
β₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Vast218]
#27886164 - 08/01/22 09:31 PM (1 year, 5 months ago) |
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The mycelium on these plates is looking messy. I don't know if the discolorations are from the colored pieces of agar and just bleeding out or if the mycelium is producing these colors like the red in picture 1. I think I see some blue on the second plate but can't tell what color is showing in the third plate.
The growth pattern of all of these cultures is off IMO. I would take a few transfers from the second overgrown plate and the third one if this is all I had to work with. Take smaller transfers if possible like just plucking a bit of mycelium off the plate. If you have more spores then start a couple new germination plates, just in case that all of this is mold.
What is your agar recipe, type and percentage of your nutrients?
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 5
#27886254 - 08/01/22 11:35 PM (1 year, 5 months ago) |
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Ho'okipa recovering nicely from getting too dry.
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Vast218
Learning


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27886413 - 08/02/22 05:45 AM (1 year, 5 months ago) |
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Quote:
Baba Yaga said: The mycelium on these plates is looking messy. I don't know if the discolorations are from the colored pieces of agar and just bleeding out or if the mycelium is producing these colors like the red in picture 1. I think I see some blue on the second plate but can't tell what color is showing in the third plate.
The growth pattern of all of these cultures is off IMO. I would take a few transfers from the second overgrown plate and the third one if this is all I had to work with. Take smaller transfers if possible like just plucking a bit of mycelium off the plate. If you have more spores then start a couple new germination plates, just in case that all of this is mold.
What is your agar recipe, type and percentage of your nutrients?
Thanks you. I will try to start a few new cultures just in case.
Plate 1 has that pink reddish color, quite beautiful really. The second plate is white, plate 3 is gray. Really weird! Maybe I stumbled upon some really cool pan species that makes funky mycelium 
The plates are pre pored, purchased from a store. One thing I did that may have been wrong was that I dropped pieces of the foil I used for the print directly in the agar plate and left it there, so I had pieces of foil and thick black spore prints on the plate. I should probably have done just a quick swipe, not sure is it matter but the presence of foil and the high spore density may have an impact on the growth.
Plates in the pics are second transfers and still have the same color patterns though.
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: Vast218] 7
#27886712 - 08/02/22 09:51 AM (1 year, 5 months ago) |
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Some updates from my end....
I grew out BabaYaga's Pan Cambos and they are solid:

On another note, I have a handful of bispo trays about to fruit. All of these trays have been pasteurized via my new steam pasteurizer which is more reliable than Instapot - I am not finding any sprouting seeds in my trays, as one indicator of successful and complete pasteurization. But, I noticed something that just confirms my years long fear of seeds in straw. My fear being that these are vectors for contamination. You effectively have uncolonized grain spawn in your trays sitting out in open air and in contact with things not sterilized. As far as I know, the current consensus when growing any actives (cubes, pans, whatever) is to use a top layer of substrate to protect the grains, and we do that even though grain spawn is already fully colonized and best-able to defend itself. So it stands to reason that having wet grains that aren't colonized just sitting out is high-risk. I have heard people say the grains will be ok because they will be so well-colonized by bacteria (after pasteurization) that the bacteria will defend against molds and pathogenic bacteria but I haven't been convinced about that.
So take a look at this Bispo tray. I did use a top layer. This is straw/verm/manure pasteurized to 165F internal and held there 2 hours, then aged in the bag 5-7 days to let the bacteria really re-colonize. That spot in the center is a straw grain, not my rye grain spawn. Note how the mycelium has avoided it. This makes me want to continue testing with shredded hemp in place of straw. I have had good success with the hemp but I haven't done enough side-by-sides to be able to conclusively say how it compares to straw. I do worry the PH may be different, and the nutrient profile as well. I need to be more scientific with the tests and now that I have eliminated pasteurization inconsistency (by switching from Instapot to my home made steamer), I can now do that. I'll post the results.
Here is the tray with the straw seed in it:

Another bispo tray that didn't happen to have any straw grains on the surface:
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