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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27806612 - 06/05/22 06:30 AM (1 year, 7 months ago)

Thanks for the report Sage! Your description of the alien worlds sounds extremely cool and actually gave me shivers! I imagine it was very dream-like?

I've only had a decent Bisporus dose once, and they sat for a while so they weren't at prime potency, but the smoothness of it is hard to really put into words. I knew I was coming up but I was doubting it at the same time due to the lack of any discomfort.


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OfflineYoshiTrainer
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 6
    #27806773 - 06/05/22 09:26 AM (1 year, 7 months ago)

Harvested the first tub of Pan cyan 'Alabama' last night. For the first pan harvest of the year, not too bad, a good starting point.





Second tub will go tonight.



I'll let them try for a 2nd flush just to see what they look like then outside they go.


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InvisibleSwabMarleyS
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] * 1
    #27806846 - 06/05/22 10:54 AM (1 year, 7 months ago)

Nice looking haul there buddy :thumbup:


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] * 1
    #27806900 - 06/05/22 11:43 AM (1 year, 7 months ago)

Quote:

YoshiTrainer said:
Harvested the first tub of Pan cyan 'Alabama' last night. For the first pan harvest of the year, not too bad, a good starting point.







Nice!!


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Offline0t0lerance
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27807002 - 06/05/22 01:14 PM (1 year, 7 months ago)



2 days progress of Estero.

I have a tendency to start over from spores as I'm seeing the cottony tomentose growth.. not the wispy type of growth :frown:

I do have 6 plates of agar going as I first made some tranfers just in case something got contaminated. However all of the plates seem to display tomentose growth.. I'll wait a bit longer.. if it's not fast I'll toss it all and start over again.


Edited by 0t0lerance (06/05/22 01:21 PM)


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: 0t0lerance]
    #27807009 - 06/05/22 01:20 PM (1 year, 7 months ago)

Nothing wrong with that growth. For example, Bisporus always looks like shit on grains but after spawning it takes off with beautiful growth.


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Offline0t0lerance
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27807014 - 06/05/22 01:24 PM (1 year, 7 months ago)

Quote:

Adas said:
Nothing wrong with that growth. For example, Bisporus always looks like shit on grains but after spawning it takes off with beautiful growth.




Hm, yes, although I could remember something Asura wrote about tomentose not being worth the risk.. and I don't want to mess up my first grow. Hmm.. I'll make some more bags of substrate just in case my first try fails, as least I have some backup then (oh btw did you notice my pm?)


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: 0t0lerance]
    #27807061 - 06/05/22 01:59 PM (1 year, 7 months ago)

Holy shit mate, thanks for notifying me! The PM icon is not blinking so I was completely oblivious!

What is the difference between "cottony" and "tomentose" growth? Arent they the same thing? Im only aware of tomentose/rhizo.


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OfflineJXAllen
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27807312 - 06/05/22 05:49 PM (1 year, 7 months ago)

I've got a couple pan cyan peace river plates I started a couple weeks ago. One has a lot of bacteria, but also OK looking mycelium in spots I think I can transfer away from the bacteria. The other plate only has two spots growing. Both are along the steak, but I'm not sure it's good mycelium. It took about a week longer to start growing. I've never grown a pan, all of my other cultures have been psilocybe(cube and tamp). I'm looking for some feedback on if any of this looks transferable?


--------------------
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OfflineMr.Giggles
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Re: Official Copelandia/Panaeolus Thread [Re: JXAllen] * 1
    #27807600 - 06/05/22 09:34 PM (1 year, 7 months ago)

These pan cincts "Warren Co, PA" weren't forming pearls on agar or grains really but it's quickly formed them in the substrate. They're roughly pea-sized. And I remembered incorrectly, they don't exactly form thick white ropes of mycelium that shoot up to the fruiting body, it's more like whispy mycelial lightning that strikes the surface and harnesses the power of the heavens into a small mushroom. I think the substrate was too moist or compressed too tightly, and this is a bit on the thick side for pans, ill use half as much sub next time. It was starting to get slightly anaerobic towards the bottom of the bin but I sat the shoebox on top of a canning jar lid to deform the bottom and allow some air to penetrate the sides and bottom. It took off after that.







And this is a jar of the cincts i found growing literally in the backyard of the local police station πŸ˜† I'll spawn these soon to a clear shoebox so I can get pics of the pearls. I have some in a couple black food containers I'm trying to fruit, may need more FAE, they've been in fruiting conditions for about a week and no pins yet.





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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: JXAllen]
    #27807670 - 06/05/22 10:53 PM (1 year, 7 months ago)

Quote:

JXAllen said:
I've got a couple pan cyan peace river plates I started a couple weeks ago. One has a lot of bacteria, but also OK looking mycelium in spots I think I can transfer away from the bacteria. The other plate only has two spots growing. Both are along the steak, but I'm not sure it's good mycelium. It took about a week longer to start growing. I've never grown a pan, all of my other cultures have been psilocybe(cube and tamp). I'm looking for some feedback on if any of this looks transferable?





6:00,on the first plate and 12:00 on the second is what I’d suggest. Any reason those plates look so contaminated? Looks like more than just bacterial contams.  Was this from a syringe or print?


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OfflineJXAllen
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27807687 - 06/05/22 11:10 PM (1 year, 7 months ago)

Quote:

Hindsight said:
6:00,on the first plate and 12:00 on the second is what I’d suggest. Any reason those plates look so contaminated? Looks like more than just bacterial contams.  Was this from a syringe or print?




Thanks for the reply! I'll take my transfers from those spots. They are from a print. I used an inoculation loop, which was flame sterilized(dab torch) between plates and cooled in the receiving plate before scraping the print and making a zig-zag streak on the plate. I made three plates. The first is the bacterial plate, the second never grew anything and the third is the second pictured plate. All were made at the same time, but the one without the bacteria first showed visible growth about a week after the bacterial one. I'm not sure if the bacteria are from my technique of the print.

Here they are a week ago. You can still see the streak on the second picture. The denser spot is the starting point of the streak.



EDIT...

I went ahead and took transfers tonight. I ended up taking three transfers from both plates. Two each from the ones recommended and one additional from elsewhere.

The bacterial colonies in the first plate are being overgrown by the mycelium. I took two transfers from the mycelium culture at 6 o'clock(from the post above) and one from the culture near the large bacterial colony.



On the second plate I took two transfers from the 12:00 culture and one from the smaller, denser one.


--------------------
There are only 10 types of people in the world, those that understand binary and those that do not.
Cultures I'm workin on


Edited by JXAllen (06/06/22 05:21 AM)


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: 0t0lerance] * 1
    #27807989 - 06/06/22 07:25 AM (1 year, 7 months ago)

Quote:

0t0lerance said:


2 days progress of Estero.





I'm convinced some shroomery people are adding a couple tablespoons of meth to their grain spawn jars because in all my grows I have never once had that kind of jump-off in anything close to two days lol. After 6 or 7 days, I usually see jump-off that extends to about 3 grain-widths away from the wedge, max. And at 2 days, usually all I see is some fuzz extending out less than 1mm from the agar wedge. This is on oats and rye. I always shake at day 7 regardless of what I see in the jars, and they take off from there.... the growth is an exponential curve. My jars almost always finish at around 14 days which I know is good/average, but I still get jealous when I see jump-off like shown above and wonder if there's something I could do to speed it up.

What do you guys see, on average, in terms of jump-off and total colonization time?


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Offline0t0lerance
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27808012 - 06/06/22 07:45 AM (1 year, 7 months ago)

Quote:

Hindsight said:
Quote:

0t0lerance said:


2 days progress of Estero.





I'm convinced some shroomery people are adding a couple tablespoons of meth to their grain spawn jars because in all my grows I have never once had that kind of jump-off in anything close to two days lol. After 6 or 7 days, I usually see jump-off that extends to about 3 grain-widths away from the wedge, max. And at 2 days, usually all I see is some fuzz extending out less than 1mm from the agar wedge. This is on oats and rye. I always shake at day 7 regardless of what I see in the jars, and they take off from there.... the growth is an exponential curve. My jars almost always finish at around 14 days which I know is good/average, but I still get jealous when I see jump-off like shown above and wonder if there's something I could do to speed it up.

What do you guys see, on average, in terms of jump-off and total colonization time?




I followed Spitball's Quick Rye Prep (contains some calcium sulphate). Use rye intended for human consumption. Apart from that the agar is LME with some soy peptone. The agar and grains were prepared a few days before I used them.. thats it.. I didn't do nor added anything fancy


Edited by 0t0lerance (06/06/22 07:46 AM)


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OfflineSirPsycho
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Re: Official Copelandia/Panaeolus Thread [Re: 0t0lerance] * 2
    #27808025 - 06/06/22 08:07 AM (1 year, 7 months ago)



Pan cyan going to rye and straw pellet bottles


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27808178 - 06/06/22 10:12 AM (1 year, 7 months ago)

Quote:

Hindsight said:
Quote:

0t0lerance said:


2 days progress of Estero.





I'm convinced some shroomery people are adding a couple tablespoons of meth to their grain spawn jars because in all my grows I have never once had that kind of jump-off in anything close to two days lol. After 6 or 7 days, I usually see jump-off that extends to about 3 grain-widths away from the wedge, max. And at 2 days, usually all I see is some fuzz extending out less than 1mm from the agar wedge. This is on oats and rye. I always shake at day 7 regardless of what I see in the jars, and they take off from there.... the growth is an exponential curve. My jars almost always finish at around 14 days which I know is good/average, but I still get jealous when I see jump-off like shown above and wonder if there's something I could do to speed it up.

What do you guys see, on average, in terms of jump-off and total colonization time?




Same here brother. My absolutely fastest culture was this Aussie Cyan culture (forgot the exact name lol) and even that wasnt as fast as what many folks claim they get. God knows what causes this, but seems to me it's purely genetic.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #27808187 - 06/06/22 10:16 AM (1 year, 7 months ago)

That's what I've been thinking too but I'd think I would have stumbled across some fast genetics at least ONCE by now haha. The important thing is that the jars are finishing in a normal amount of time, but it's still a curious thing.


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Offline0t0lerance
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27808352 - 06/06/22 12:44 PM (1 year, 7 months ago)

Quote:

Hindsight said:
That's what I've been thinking too but I'd think I would have stumbled across some fast genetics at least ONCE by now haha. The important thing is that the jars are finishing in a normal amount of time, but it's still a curious thing.




Well, I'm a beginner and actually expect the mycelium jumping on grain the very next day.. I haven't seen it not do that to be honest. Moreover, I thought panaeolus is a bit faster than cubensis and woodlovers, so I was a bit disappointed it "just" moved there in 2 days. Maybe the altitude? (below sea level here) can't be my skill.. I'm a noob with a SAB.


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Re: Official Copelandia/Panaeolus Thread [Re: 0t0lerance] * 3
    #27808457 - 06/06/22 02:32 PM (1 year, 7 months ago)

Temperature plays a major factor. Some people's houses are 65F, some are 85F.

There are even outliers beyond those but that will be rare.

Also gas exchange hole size. I recently started using a larger drill bit after noticing my filter sticker lids were colonizing way slower than tyvek lids. That made a difference.



--------------------

Panaeolus Bisporus


Edited by karri0n (06/06/22 02:34 PM)


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: karri0n] * 1
    #27808500 - 06/06/22 03:03 PM (1 year, 7 months ago)

Good point on the GE hole. I size mine based on how much moisture I'm losing to evaporation. Kind of like setting up and adjusting a monotub. I find that with the SFDs I use, I can only have a 1/8" hole in the lid or my grains start getting dry and growth stalls out. I'll have to play around with it a bit more though - it's been a while. I used to do a 1/4" hole. My temps are 74F in the area I let my jars colonize.


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