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InvisibleBaba Yaga
♥ coir grower

Registered: 09/13/20
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Re: Official Copelandia/Panaeolus Thread [Re: Shroomguy101]
    #27772132 - 05/11/22 01:18 AM (1 year, 8 months ago)

Yeah I can see the pins. Your casing is loamy soil right? How would you describe the current moisture saturation of your casing? It does look
dry. IMO it is important to give lots of moisture/water in the beginning of the grow.

The point of watering your substrate is to provide water for your fruits. Mushrooms are like sponges and most of the cells that will make
up the grown out fruit are already present in the little pins. Mushroom fruits bodies do therefore mainly grow by pumping up those cells with
water. That is the reason why mushrooms can appear overnight.

If you water with a syringe than you mainly water the substrate surface and more so closer and around the pins you want to promote in growth.

What kind of fruiting chamber is this? Shoebox?


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InvisibleShroomguy101
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27772137 - 05/11/22 01:31 AM (1 year, 8 months ago)

It is loamy soil. And yes it is a shoebox. The casing is not too dry currently but it could use a little moisture. If I water with a syringe, i should water around the pins, but not let the water touch the caps - correct?


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OfflineBra
grow panaeolus, it's easy


Registered: 07/12/19
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #27772141 - 05/11/22 01:43 AM (1 year, 8 months ago)

Quote:

Guerrilla said:
Anybody have a spare print they are confident in that they wouldn't mind sharing? Would be greatly appreciated. Tried a couple different prints..bacterial disaster. Haven't had the pleasure of trying pans yet.



I've sent you a PM. Check this out.


Quote:

Baba Yaga said:
Quote:

Bra said:
Thanks.
I inoculate horse poo with straw 1:1 just in jars. Than make casing layer (peat based substate for flowers) on it and under it.
(but I gonna try your casing layer recipe now, peat+verm with 1 teaspoon of lime, ready to case it right now with tek I usually do but use your casing layer recipe).

Than I let it consolidate for 1-2 days.
And put into fruiting chamber with temperature 25-28C

It grows like that










Looking good Bra, you do have great success there.
Your jars look a lot like angams hay manure substrate which he does with jars as well.

Looking at these gorgeous trays in the tub. Have you got a fogger feeding into the tub or anything? I and probably a few other would be
interested to hear about your fruiting method.

Thanks for the information, now I know what you are doing, good stuff :thumbup:



The funny thing is that is not angam's method, he just popularized it here.
He started to grow shrooms in 2019.
This tek is more older. I've tried to grow pans in 2012 with this tek.
(and I would like to know who discovered this tek, what shroomer, I don't know who was first? whom this tek belongs to?)
I just typed in the search "how to grow panaeolus" and this was the first TEK I found.
And I love it more than tek with grains, to be honest. The mycelium looks very cute in the jars with manure-straw and I love that there is very high successful rate. Manure is quite resilient to contams I would say.


--------------------
Bra's spores giveaway


Edited by Bra (05/11/22 02:12 AM)


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Offlinebunsie
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Re: Official Copelandia/Panaeolus Thread [Re: Bra] * 1
    #27772332 - 05/11/22 07:18 AM (1 year, 8 months ago)

Recent harvest and grow on coir/manure pellets.
The stems aren't as long as I see here on the sub.
Anything I can improve?



Edited by bunsie (05/11/22 07:24 AM)


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: bunsie]
    #27772349 - 05/11/22 07:42 AM (1 year, 8 months ago)

Nice grow! Short stems can be due to genetics (some varieties are just shorter than others), or lack of FAE, in my experience.


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Offlineshr00mFer
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27772353 - 05/11/22 07:45 AM (1 year, 8 months ago)

Are these ready for picking? If interested there is pic in my gallery (and post here also) to compare it to picture from 2-3 days ago. It seems fruits are not growing anymore.



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OfflineBra
grow panaeolus, it's easy


Registered: 07/12/19
Posts: 344
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Re: Official Copelandia/Panaeolus Thread [Re: shr00mFer]
    #27772379 - 05/11/22 08:26 AM (1 year, 8 months ago)

Quote:

shr00mFer said:
Are these ready for picking? If interested there is pic in my gallery (and post here also) to compare it to picture from 2-3 days ago. It seems fruits are not growing anymore.





I would harvest now.


--------------------
Bra's spores giveaway


Edited by Bra (05/11/22 08:36 AM)


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OfflineRoscoepan
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27772576 - 05/11/22 11:55 AM (1 year, 8 months ago)

Ok, I must have just taken too little. It's been challenging to figure out dosage for the different varieties and I thought Necd was closer to 1gr. But after searching and reading again I see 3-4gr is appropriate for them. I doubt I had white based on the quantity of spores they were dropping.

My question about white Necd because my first trip was very light and I barely noticed it versus the GT or Cambodian. My time is limited and after a long grow I really hope it pays off with a good journey.

I have plenty of prints (Peace River, Hawaiian, Necd, Jam) to work with so I'll get back to it.


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Offlinechefnickwbig10
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Premixes [Re: Roscoepan]
    #27772646 - 05/11/22 01:05 PM (1 year, 8 months ago)

I’m a new grower who has failed twice at growing pan cyans. I’m trying to do this as simple as possible.I actually successfully inoculated rye grain bags but got trich after spawning them with substrate I bought online.
I wanted to to know if anyone has come across any premixed substrates and casing that is pre-pasteurized that will work for cyans.
I have no idea how to use a pressure cooker and am willing to pay the extra. I’ve grown cubes 3x’s w/out issue in a Martha tent and am itching for cyans. Any help would be appreciated. Thanks


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Offlinejomanda1990
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Re: Premixes [Re: chefnickwbig10]
    #27772696 - 05/11/22 01:48 PM (1 year, 8 months ago)

Quote:

chefnickwbig10 said:
I’m a new grower who has failed twice at growing pan cyans. I’m trying to do this as simple as possible.I actually successfully inoculated rye grain bags but got trich after spawning them with substrate I bought online.
I wanted to to know if anyone has come across any premixed substrates and casing that is pre-pasteurized that will work for cyans.
I have no idea how to use a pressure cooker and am willing to pay the extra. I’ve grown cubes 3x’s w/out issue in a Martha tent and am itching for cyans. Any help would be appreciated. Thanks




Sorry I don't know about premixed substrates, but maybe it's helpful if I clarify that you don't need a pressure cooker to pasteurize. You need a few glass jars, a large pot and a thermometer with a submersible probe. The idea is to heat the water on the stove so that, in turn, it heats the substrate in a controlled manner. You keep a close eye on the temps as they rise; you want to cut the heat in such a way that the substrate climbs to about 60+ degree Celsius but stays below 75 Celsius. Check Frank Horrigan's guide for more details. In short, you just need to pay less than ten bucks for a digital thermometer and learn a rather straigtfoward process, it may be worth it for you in the short and long run. For manure-based substrates, however, you should probably extend the pasteurization time well beyond just one hour


--------------------


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Offlinechefnickwbig10
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Re: Premixes [Re: jomanda1990]
    #27772714 - 05/11/22 02:08 PM (1 year, 8 months ago)

Ok I appreciate the help. I’m reading over all these posts and nearly everyone is making their own substrates and such so I figure I’m probably gonna have to figure out how to do it eventually. Cyans just seem like such a complicated endeavor right now. But thanks again!


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OfflineShinyWartortle
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I'm a teapot Unread Journal

Registered: 04/14/22
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Re: Premixes [Re: chefnickwbig10]
    #27772742 - 05/11/22 02:33 PM (1 year, 8 months ago)

Quote:

chefnickwbig10 said:
Ok I appreciate the help. I’m reading over all these posts and nearly everyone is making their own substrates and such so I figure I’m probably gonna have to figure out how to do it eventually. Cyans just seem like such a complicated endeavor right now. But thanks again!





I haven't successfully had them fruit yet but so far I've got no contams. I just pasteurized cow manure the same way you do with coir. (boiled water, poured it over it in a container with the lid on and let it sit for 4 or so hours.) After that I spawned my pans to the bulk and it successfully colonized the manure/coir and I'm waiting for fruits. I didn't think it would work and the leftover manure did end up molding but my shoebox hasn't. Maybe it will fruit and maybe it won't so I won't tell you to do it the way I did but so far it's working. Lol


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OfflineCrackatoa
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Re: Premixes [Re: ShinyWartortle] * 2
    #27772747 - 05/11/22 02:39 PM (1 year, 8 months ago)

A sous vide is the easiest way to pasteurize everything


--------------------

Crack's Tek's and Shit



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Offlinekarri0n
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Re: Premixes [Re: Crackatoa] * 1
    #27772990 - 05/11/22 06:23 PM (1 year, 8 months ago)

A crock pot/slow cooker also works almost as well as a sous vide. I don't remember whose Tek it is, but one of the top pan teks on here utilizes a crock pot on the warm setting for 12 hours.

I've done it several times with different sized bags at this point. I stopped using the thermometer, because it is always perfect 150F, and the risk to poking a hole in the bag with the probe is about 1000 times higher than the temp being off for me at this point.

You will need to test your own crock pot to be sure.

Bonus fact: Ziploc bags are good to 212F, so you don't always need to use a myco bag if you aren't pressure cooking. Ziploc actually supports using them for sous vide.


--------------------

Panaeolus Bisporus


Edited by karri0n (05/11/22 06:27 PM)


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InvisibleWall.E
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Registered: 06/05/20
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Re: Premixes [Re: karri0n]
    #27773119 - 05/11/22 08:00 PM (1 year, 8 months ago)

That’s how I pasteurize too. Gallon ziploc fits my casing and my instant pot fits my gallon bag. Keep warm setting overnight and bada bing bada boom. Still haven’t had any issues with that method.


--------------------
Life’s shit, but I’m loving it



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OfflineHindsight
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Re: Premixes [Re: Wall.E]
    #27773616 - 05/12/22 08:07 AM (1 year, 8 months ago)

I like Instapot pasteurization the best. Load the sub into a 2.5 gallon ziplock bag, put it into the instapot, top off with water, set to keep warm mode on 12 hour timer and done. The only problem I have run into is sometimes for whatever reason, if you don't have enough water touching the bottom and sides of the pan (because the substrate bag is pressed up against it instead), the pot won't heat up to the full target temp. I've noticed this just by feeling the outside of the lid of the instapot after a few hours and finding it cooler than it normally is. I've done some checks with an infrared thermometer and found that the outside top of the pot should be 130F (correlating to an internal temp of around 150F). If it isn't, you know you have an issue so you open the pot, shift the bag around a bit, top up with more water, etc, and try again. The proof that the pasteurization didn't work is when you get ink caps or other mushrooms growing in your trays (not to mention contamination though there are multiple things that could cause that instead of pasteurization failure and not pasteurizing at all isn't a guarantee that your grow will contaminate).

I tried the sous vide method before going with the Instapot method. The issues I had with it were that the water bath would evaporate quickly (needing to be topped off a lot) and too much heat was escaping from the top of the sub, preventing it from reaching and holding target temps. I know this could be fixed by not using an open-pot water bath and instead modifying a plastic cooler with a lid on it so you could put your sous vide down in it, and also weighting your substrate bags so they sink to the bottom of the bath. I also had issues with the narrow range of water depth that the sous vide device can work with. Again that could be solved by modifying a cooler. I just went instapot because it was easier and more set-and-forget - I just leave it run overnight. You are limited to about 9qts of substrate using the Instapot method Sous vide would allow for a lot more than that.


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OfflineMycoplex
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Re: Premixes [Re: Hindsight] * 4
    #27774007 - 05/12/22 12:51 PM (1 year, 8 months ago)

An update on cloning the panaeolus cyanescens pseudo-stones from earlier in the thread.

I cut cross-sections of the pseudo-stones and cloned them to 10 plates.  The plates are beginning to show the same pattern that the donor plate showed before producing the structures.

The general mycelium growth here appears to be tomentose with a sort of secondary wave/fin of mycelium.  Right above the areas where the pseudo-stones are expected to pop up, I see droplets of water (does not appear to be metabolites).  There also happens to be condensation on the plates, separate from the droplets I'm describing.  I expect the area where the droplets form to eventually produce the same structures just like the donor plate.



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OfflineMoopers
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Registered: 05/08/20
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Re: Premixes [Re: Mycoplex] * 2
    #27774123 - 05/12/22 02:05 PM (1 year, 8 months ago)

Not a great pic, but I'm excited for my first PHV pins!



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Invisiblehummingbird

Registered: 06/29/14
Posts: 2,134
Re: Premixes [Re: Mycoplex] * 1
    #27774127 - 05/12/22 02:06 PM (1 year, 8 months ago)

That's cool! Whenever I have had plate stones show up with stone producers they make little water droplets like that where they form.

I'm interested to see what happens when you put some of this to grains, those pseudo-stones you had were crazy looking. Would be awesome to see a culture that put out a decent amount of pan stones like that in a jar...though it would be intimidating to try them.


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OfflineMycoplex
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Re: Premixes [Re: hummingbird]
    #27774140 - 05/12/22 02:23 PM (1 year, 8 months ago)

Quote:

hummingbird said:
That's cool! Whenever I have had plate stones show up with stone producers they make little water droplets like that where they form.

I'm interested to see what happens when you put some of this to grains, those pseudo-stones you had were crazy looking. Would be awesome to see a culture that put out a decent amount of pan stones like that in a jar...though it would be intimidating to try them.




Thanks for checking it out hummingbird.  There are a couple more pics I just took that
show a more developed version of the structures on the new plates.

In both of these plates, the masses that are developing outward from the center are all new structures, the donor psueduo-stone was placed in the center.



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