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Mr.Giggles
Time bandit



Registered: 12/19/20
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Re: Official Copelandia/Panaeolus Thread [Re: arpeggiator]
#27741423 - 04/19/22 05:30 AM (1 year, 9 months ago) |
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Quote:
arpeggiator said: Hey everyone. This is the first time I'm growing Panaeolus cyanescens. The mycelium have little bumps on them? Is this normal?

Keep an eye on that and don't throw it out yet, it could be pins or sclerotia, they kinda start out looking like that with other species of pans.
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
#27741477 - 04/19/22 06:35 AM (1 year, 9 months ago) |
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Quote:
ekabpils said: Hi!
This is the first flush of BVI:

Great work! I love BVI.
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Timestop413
Stranger



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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles]
#27741586 - 04/19/22 08:13 AM (1 year, 9 months ago) |
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Quote:
Mr.Giggles said:
Quote:
arpeggiator said: Hey everyone. This is the first time I'm growing Panaeolus cyanescens. The mycelium have little bumps on them? Is this normal?

Keep an eye on that and don't throw it out yet, it could be pins or sclerotia, they kinda start out looking like that with other species of pans.
On my Pan Estora I see this on agar and it usually stalls out if im seeing it, but then I just transfer to grain and it grows well. I dont know what it is but it has not caused any issues in my setup.
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shr00mFer
Stranger



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Re: Official Copelandia/Panaeolus Thread [Re: Timestop413]
#27742115 - 04/19/22 02:08 PM (1 year, 9 months ago) |
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Hi guys. I pasteurized some straw/manure/verm 2 days ago. Used it but I have 2 jars extra. Is it still ok to use it? Waiting for some pan jambo grain to colonize fully (some 4-5 days more) before I was meaning to use them
How long can pasteurized substrate stay "on hold" before mixing it up with grain? Should I re-pasteurize it after some number of days?
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Baba Yaga
♥ coir grower

Registered: 09/13/20
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Re: Official Copelandia/Panaeolus Thread [Re: shr00mFer]
#27742145 - 04/19/22 02:28 PM (1 year, 9 months ago) |
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Letting substrate sit on a shelf for a week is what some people do on purpose to bring the microbe count up. The smell will intensify but that is nothing to worry about. I kept airtight containers with pasteurized substrate & casing in the fridge for a month and it worked fine, even when opened to use some and then put back in.
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Snazz
Polymath



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Posts: 1,584
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: shr00mFer]
#27742146 - 04/19/22 02:28 PM (1 year, 9 months ago) |
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Depending on how you pasteurize, it can best practice to let the thermophilic bacteria recolonize the outer substrate layer over the course of days anyways
(steam partially sterilizes the sub)
If you sous vide, it never goes above 140-180°F and don't have that issue
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Crackatoa
Stranger in a strange land



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Re: Official Copelandia/Panaeolus Thread [Re: Snazz]
#27742291 - 04/19/22 04:05 PM (1 year, 9 months ago) |
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Quote:
Snazz said: Depending on how you pasteurize, it can best practice to let the thermophilic bacteria recolonize the outer substrate layer over the course of days anyways
(steam partially sterilizes the sub)
If you sous vide, it never goes above 140-180°F and don't have that issue
Are you saying with the Sous Vide there is no need for the wait?
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Sagescruffy
CH



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Re: Official Copelandia/Panaeolus Thread [Re: Crackatoa]
#27742370 - 04/19/22 05:05 PM (1 year, 9 months ago) |
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Bisporus “pin porn” sort of haha:
-------------------- Love.  
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Snazz
Polymath



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Posts: 1,584
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: Crackatoa]
#27742383 - 04/19/22 05:10 PM (1 year, 9 months ago) |
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Quote:
Crackatoa said:
Quote:
Snazz said: Depending on how you pasteurize, it can best practice to let the thermophilic bacteria recolonize the outer substrate layer over the course of days anyways
(steam partially sterilizes the sub)
If you sous vide, it never goes above 140-180°F and don't have that issue
Are you saying with the Sous Vide there is no need for the wait?
Your bath never goes high enough to kill the outer "good bacteria" it just slowly heats everything until the middle reaches the bath temp. No need to recolonize
Steam nukes at high temp until it heats the core to pasteurization temp, so there's partial sterilization and risk of contam if S2B immediately
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Crackatoa
Stranger in a strange land



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Re: Official Copelandia/Panaeolus Thread [Re: Snazz]
#27742520 - 04/19/22 06:29 PM (1 year, 9 months ago) |
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Awesome, I use a sous and still giving it 5-7 days, and a 10 day consolidation time.
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Hongosamongus
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
#27742525 - 04/19/22 06:32 PM (1 year, 9 months ago) |
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Could you please explain the humidity system that you have installed in the lower photo? Is it just two lines from an aquarium pump? Airstones installed? Sorry if this has been explained elsewhere in the thread, but I couldn't find it. Also...what is bubble wrap tek and what's the theory behind it?
-------------------- I'm a stranger here myself.
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shr00mFer
Stranger



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Re: Official Copelandia/Panaeolus Thread [Re: Snazz]
#27742988 - 04/20/22 12:27 AM (1 year, 9 months ago) |
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Quote:
Snazz said: Depending on how you pasteurize, it can best practice to let the thermophilic bacteria recolonize the outer substrate layer over the course of days anyways
(steam partially sterilizes the sub)
If you sous vide, it never goes above 140-180°F and don't have that issue
Jars (with only substrate) submerged in water almost all the way on the stove. Heated until some temperature, then turn off. It was on 160-175 F for an hour or two. After that jars are put on the shelf. So I am ok to use it in a 3-4 days (7 in total)? Nice. Thank you all.
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: shr00mFer] 6
#27743284 - 04/20/22 08:37 AM (1 year, 9 months ago) |
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Harvested this tray of bispos today:

These are the first fruits I have harvested in over a month I think. For a while, all my cult time went into building my flow hood and that damn thing took over a month to finish and get sorted. I wasn't streaking plates or making spawn during that time so I ended up in a place where everything I had going fruited and I was left with empty shelves and an empty tent. Now I keep finding myself sitting on jars that are 100% consolidated for 1-2 weeks because I don't have time to pasteurize substrate or spawn trays. I know JOC has said he gets subpar results from spawning jars that were allowed to sit 1+ weeks past 100% colonization but am curious what your experiences have been with this? I know over on the stone producer thread, a lot of people are leaving their spawn jars for weeks or months before fruiting and still get great flushes. I realize this could be specifically because of the survival mechanism of stone producers, but I personally haven't noticed lack-luster fruiting of pans or cubes when letting my jars sit 1-3 weeks beyond full colonization. However, I haven't done any true experiments on it where I create a couple jars from the same clone and fruit one right at full colonization and fruit the other three weeks later and compare results.
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SwabMarley
Twisted Metal Head



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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27743293 - 04/20/22 09:02 AM (1 year, 9 months ago) |
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Curious to find out peoples results as well. From everything I’ve read on Pans it seems like it’s a constant race from germination to fruits. Select the fastest bit of myc then put to grain and don’t let it sit etc.
I’ve just G2G a culture that’s been sat for at least 2 weeks after full colonisation so I’ll report back what happens with those.
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elpico
Stranger

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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 2
#27743430 - 04/20/22 10:36 AM (1 year, 9 months ago) |
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That bisporus canopy is insane, dude. Two thumbs up.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27743447 - 04/20/22 10:56 AM (1 year, 9 months ago) |
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Quote:
Hindsight said: Harvested this tray of bispos today:

These are the first fruits I have harvested in over a month I think. For a while, all my cult time went into building my flow hood and that damn thing took over a month to finish and get sorted. I wasn't streaking plates or making spawn during that time so I ended up in a place where everything I had going fruited and I was left with empty shelves and an empty tent. Now I keep finding myself sitting on jars that are 100% consolidated for 1-2 weeks because I don't have time to pasteurize substrate or spawn trays. I know JOC has said he gets subpar results from spawning jars that were allowed to sit 1+ weeks past 100% colonization but am curious what your experiences have been with this? I know over on the stone producer thread, a lot of people are leaving their spawn jars for weeks or months before fruiting and still get great flushes. I realize this could be specifically because of the survival mechanism of stone producers, but I personally haven't noticed lack-luster fruiting of pans or cubes when letting my jars sit 1-3 weeks beyond full colonization. However, I haven't done any true experiments on it where I create a couple jars from the same clone and fruit one right at full colonization and fruit the other three weeks later and compare results.
Killng it again! This is a better Panopy than most of the Jake Oncid stuff with Bisporus! You just keep blowing my mind man. Unfortunately I can only give 1 upvote per post, and 1 rating. lol.
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THE MYCOSMITH
Weekend mycologist


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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#27743760 - 04/20/22 02:32 PM (1 year, 9 months ago) |
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Hey Hindsight Congratulations with (maybe) the best bisp flush to date.
I often make more spawn than i use. And I see vitality drop fast in pan's. I have used spawn after 1 month of full colonization with succes but often after 2-3 month the spawn almost look dead. Here is a good example but on pan cint. New colonized and 2 month old.
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] 2
#27743823 - 04/20/22 03:14 PM (1 year, 9 months ago) |
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@ Hindsight. Looking good. What substrate have you used in this one?
Letting spawn sit for 2-3 weeks didn't lower the performance/success rate noticeable in my experience.
Quote:
THE MYCOSMITH said: New colonized and 2 month old.

That growth looks very unusual, even the freshly colonized one. Are jars like this producing good results for you?
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Adas
Lonely Dreamer



Registered: 12/22/16
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#27743843 - 04/20/22 03:27 PM (1 year, 9 months ago) |
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Hmmm I let my Bunnell spawn sit for 2 weeks or so, and after putting it to bulk there is ZERO activity after 2 days. It's fucked.
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Sagescruffy
CH



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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#27743911 - 04/20/22 04:18 PM (1 year, 9 months ago) |
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Awesome Bisporus canopy! Have you sampled any yet?
-------------------- Love.  
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