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Bernard
Cultvr - Not Necessarily Trusted



Registered: 05/08/16
Posts: 226
Last seen: 2 years, 7 months
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Re: Official Copelandia/Panaeolus Thread [Re: Peteyboy]
#23931594 - 12/16/16 06:20 AM (7 years, 1 month ago) |
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I've lost count of the number of transfers, but it's in the neighborhood of 5 or 6.
I know I'm asking dumb questions, but I thought the idea of transferring from agar plate to agar plate was to ensure there's no other bacteria or anything else. Sure, I saw some nasty stuff when I first dropped from the syringe, but I haven't seen a contamination in the last 3 or 4 transfers. But they still grow non-uniform and a little "funny".
I have some cube samples that are perfect with rhizo growth and perfectly round etc. I know this is a different creature, but maybe the problem is I just don't know exactly what I'm looking for.
I tried to noc some PF style cakes when I first got the syringe and everything went to contamination. So, I'm thinking there must be something else in that syringe (and yes it came from a sponsor).
What does pan cam like better as growing medium? Potato or malt dextrose? Does it matter?
Thanks for all of the help. You guys have been very patient. I'm getting frustrated and you are keeping me grounded.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 6 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Bernard]
#23932100 - 12/16/16 10:54 AM (7 years, 1 month ago) |
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Quote:
I know I'm asking dumb questions, but I thought the idea of transferring from agar plate to agar plate was to ensure there's no other bacteria or anything else. Sure, I saw some nasty stuff when I first dropped from the syringe, but I haven't seen a contamination in the last 3 or 4 transfers. But they still grow non-uniform and a little "funny".
this "funny" look is mold. pan spores are hard to keep clean, if you see any break in growth it's not pan myc. the last three you posted look clean but they could still be hiding something. I would lower your nutes so it's easier to see rhizomorphs. with your dirty plates you likely just transferred mold and it outgrew your pans. throw out anything that isn't organized.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#23934895 - 12/17/16 09:31 AM (7 years, 1 month ago) |
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I transferred from these plates yesterday (Goliath)
This is pan jam, really weird growth, not so fluffly 
This is a jar i transferred one of the pan goliath, seemed clean on agar, as it grew on grain it changed, even the mycelium looks super weird 
This is the test tub i was talking about, going green, yuck
I never had the greeny meanie in any of my grows, my thoughts are that it was intertwined with the pan mycelium. (Even though i cleaned it up for a while now)
And pans always bruise blue for me, does it happen to you guys too? (especially when i keep them on agar for a while.

Such a pain in the ass lol, these are like on their 15th transfer or something.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 6 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#23935463 - 12/17/16 01:36 PM (7 years, 1 month ago) |
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those last two look like mold
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#23935542 - 12/17/16 02:05 PM (7 years, 1 month ago) |
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It does now, but it looked like Pan mycelium before it started bruising and looking like that
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#23935547 - 12/17/16 02:06 PM (7 years, 1 month ago) |
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It does look like kind of powdery, splotchy and uneven...I had that look going on, on some cinctulus plates I was working on, then it disappeared, but one I dropped into grain it took over quick...
Edited by Peteyboy (12/17/16 02:07 PM)
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: Peteyboy]
#23935554 - 12/17/16 02:10 PM (7 years, 1 month ago) |
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It only morphs like that when it nearly fully colonises the plate.
It looked the the first pictures at first, then it becomes like the last one.
I guess ill just have to keep cleaning and cleaning, its over 15 transfers right now
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 5 hours, 34 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#23935567 - 12/17/16 02:18 PM (7 years, 1 month ago) |
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15? Is that from a Clone or spores? Hopefully from a clone or your just playing with it.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: wtfcrazymofo]
#23935590 - 12/17/16 02:26 PM (7 years, 1 month ago) |
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Its spore, its better to germinate on agar, and clean away till you get a uniform growth.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
Edited by Teemo 6T3 (12/17/16 02:27 PM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 6 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#23935709 - 12/17/16 03:10 PM (7 years, 1 month ago) |
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pans seem like such a pain in the ass. all of my jars I g2g'd to are growing mold... none of the colonised grain took hold but it started growing white irregularly... I wish they weren't so intertwined with contams
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#23935735 - 12/17/16 03:17 PM (7 years, 1 month ago) |
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Its funny how other noobs get so lucky and just drop a couple of drops on grain, and within 3 weeks, they already have fruits, while we're fucking stuck on agar cleaning these fuckers
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 6 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#23935744 - 12/17/16 03:20 PM (7 years, 1 month ago) |
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right? I don't get it... I do also live in a house pretty much made of mold. but that's no excuse! I'm going back to cubes for a minute. but for now, we wait. good luck to you!
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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moricz
Inky Cap Cultivator



Registered: 07/14/13
Posts: 969
Loc: EU
Last seen: 3 months, 22 days
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#23939760 - 12/19/16 06:26 AM (7 years, 1 month ago) |
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: Official Copelandia/Panaeolus Thread [Re: moricz]
#23940214 - 12/19/16 12:24 PM (7 years, 1 month ago) |
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Throwing bisporus wedges to grain tonight. Sure hope I get some printable fruits for once, or fruits at all!!!! This species has been my bane.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: d0urd3n]
#23940234 - 12/19/16 12:33 PM (7 years, 1 month ago) |
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Good luck bro, may the shroom lords be with you
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Hekate
Probiotic

Registered: 12/23/12
Posts: 68
Last seen: 6 years, 10 months
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#23942323 - 12/20/16 02:37 AM (7 years, 1 month ago) |
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What agar recipe is recommended for germination? How about for transfers? How many transfers would you expect necessary to get a good culture? Can the mycelium realistically run faster than its competitor bacteria can spread?
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Pinpapa
Stranger than most


Registered: 09/01/12
Posts: 259
Loc: Algonquin park
Last seen: 3 years, 10 months
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Re: Official Copelandia/Panaeolus Thread [Re: Hekate]
#23942519 - 12/20/16 06:56 AM (7 years, 1 month ago) |
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This Jam. was cased 6 days ago. no contams yet. One little spot is colonizing the case.daytime 69 f each night it goes to 50 in a cold room but no signs yet.
-------------------- "This isn't the right thing to do , so let's go"
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mary fairchild
Pantheist

Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: d0urd3n]
#23942631 - 12/20/16 08:03 AM (7 years, 1 month ago) |
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Quote:
d0urd3n said: Throwing bisporus wedges to grain tonight. Sure hope I get some printable fruits for once, or fruits at all!!!! This species has been my bane.
I tried a kinda Pancake style Bisporus grow where I dropped wedges on to a couple of 1/2pint jars of horse manure, wheat bran based media. This method worked for Sandos- sort of- but I got no fruiting with Bisporus.
Best wishes for your grow!
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Thanks Mary, they sure have been tricky for me.
I'll keep you guys updated.
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Shrms
Stranger
Registered: 06/18/16
Posts: 42
Last seen: 6 years, 8 months
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Re: Official Copelandia/Panaeolus Thread [Re: d0urd3n]
#23945044 - 12/21/16 12:47 AM (7 years, 1 month ago) |
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Hey guys,
because I did not geht any response to my latest question in my original thread I move here where all of you experts romp around 
I am very new to pans, my last (and first pan grow) produced some shrooms but it was not ideal at all.
Now after a little more research I have a try referring to MudaFuka's Bottle Tek and spawning to trays, according mary fairchild's tek.
I inoculated the jar 5 days ago with liquid culture of pan. tropicalis. It is a mixture of hpoo, vermiculite, BRF and gypsum with a top layer of pure dry vermiculite. I did only one jar for testing, because it was my first liquid culture syringe ever and I did not want to ruin the hole bunch.

You can see some mycelium growing in the substrate, I wonder how it is doing the next days.
Also I made two trays 5 days ago (also pan trop.),WBS spawned to a substrate (1:2) consisting of hpoo,vermiculite,gypsum (tray 1). For tray 2 I added some straw pellets to see if it makes any difference. Both substrates were pasteurized in a bag for one hour.
Tray 1 (hpoo:vermiculite:gypsum, 10:2:0.5)
 
Tray 2 (hpoo:straw pellets:vermiculite:gypsum, 10:3:2:0.5)
 
The trays were in the incubator (80°) for 5 days now, what do you think of the progress? The surface is looking a little bit dry to me, but on the sides and bottom there are plenty of water drops, also the aluminum foil on top is little bit moist. Do I have to rehydrate in any form or just wait until full colonization and apply the casing layer? I have read (do not have the reference) that someone puts a wet towel ontop (instead of aluminum) and mists if it is getting too dry.
How should I proceed now? Thanks in advance
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