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Baba Yaga
โฅ coir grower

Registered: 09/13/20
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Re: Official Copelandia/Panaeolus Thread [Re: coversall]
#27662230 - 02/17/22 07:08 AM (1 year, 11 months ago) |
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Quote:
coversall said: Hmm I'm about to mix up some pan-cake mix. But am a little low on brown rice potentially. Any reason why I shouldn't throw in some whole millet? Or even grind the millet into a flour if I need a bit extra to make the ratio up? Or should I make up the difference with manure/straw? So many possibilities!
If you are going to PC it then any flour will do, whole meal wheat flour works just as well.
Quote:
Drboomer said: LI I wish I had thrown it in an lc. I feel much more able to read an lc and tell if it's good or not. I don't have a good source for nice dry manure just composted.
Composted manure can work just as well though if the overall texture of the substrate is nice and fluffy.
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#27662252 - 02/17/22 07:44 AM (1 year, 11 months ago) |
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Quote:
Adas said:
Quote:
Hindsight said: I have used tap water, reverse osmosis filtered tap water, and bottled distilled water. I use the latter now just because it's an easy way for me to rule out any additional variables the could be responsible for something screwy going on. The fewer things I have to wonder about being the problem, the better, so I go distilled. Having said that, I've never really noticed any difference between the three water types, personally, but I do know that public water quality changes over time, and most bottled water has minerals or electrolytes added for taste (calcium, silica, etc) and I don't know how much effect these can have on mycelium.
I don't think it's "for taste", but literally so that people don't drink demineralized water. This would have bad health consequences and make you mineral deficient quickly.
Good point - I learned about that when I researched and then purchased a reverse osmosis water filter. Both R/O and distilled aren't good for people to drink due to the problem you mentioned. The R/O system I purchased has a remineralizing cartridge that adds calcium and magnesium back to the water to prevent that, but I have noticed that bottled water can have some other additives (that vary by brand) and in the ingredient list they say they are "added for taste". One example is Potassium Bicarbonate, though I have seen others. I don't know the impact (if any) that each of these added minerals would have on mycelium though, so I just choose to go with distilled since I know it works for me and others and it's one less thing I have to worry about. But again, sometimes I run out and use my R/O water that has cal/mag added back in and generally don't notice any difference so.....
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coversall
ุฅููู ุดูุงุกู ูฑููููู



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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27662255 - 02/17/22 07:46 AM (1 year, 11 months ago) |
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Quote:
Baba Yaga said:
Quote:
coversall said: Hmm I'm about to mix up some pan-cake mix. But am a little low on brown rice potentially. Any reason why I shouldn't throw in some whole millet? Or even grind the millet into a flour if I need a bit extra to make the ratio up? Or should I make up the difference with manure/straw? So many possibilities!
If you are going to PC it then any flour will do, whole meal wheat flour works just as well.
Quote:
Drboomer said: LI I wish I had thrown it in an lc. I feel much more able to read an lc and tell if it's good or not. I don't have a good source for nice dry manure just composted.
Composted manure can work just as well though if the overall texture of the substrate is nice and fluffy.
Rad, I ended up with just enough brown rice so I'll save other flours for another day!
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Baba Yaga
โฅ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: coversall]
#27662262 - 02/17/22 07:56 AM (1 year, 11 months ago) |
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Looks appetizing
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Panny
Voyager



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Re: Official Copelandia/Panaeolus Thread [Re: coversall]
#27662283 - 02/17/22 08:22 AM (1 year, 11 months ago) |
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Quote:
coversall said:
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Drboomer said: I love those little 250ml media bottles. They are perfect for small lc batches. I'm buying a ten pack at the end of the month and forgoing mason jars for lc.
My first batch of pan cakes has colonized pretty well. The only iffy parts are compost clumps I didn't break up well enough I'm going to try fruiting them anyway. Might shift my compost next run so I don't have little clumps of it.being partially frozen didn't help either. Hopefully I get at least a fruit. Dialing the Martha and my first run of cakes makes for a ton of variables up in the air at the same time.
My manure is almost entirely dry, so when preping the stuff for my pan cakes I rub it between my hands to break up any clumps!
What did you start the pancakes with? LI or LC?
Quote:
Panny said:
Hmmm, this makes sense. Anytime I see people posting pics of Pan mycelium on agar is looks a lot more dense and uniform that what I have. I also use bottled water for agar, could this also be a problem? I'm not sure if most of you use distilled water, or is tap water good enough most of the time?
I've always used tap water for everything mycological and had no trouble there. I'd start by ditching the food colouring.
What is your agar recipie?
Yeah, I bet that's it.
Current recipe: 300ml bottled water 6g agar 2g instant mashed potato flakes 1.5g light malt extract
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shr00mFer
Stranger


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Re: Official Copelandia/Panaeolus Thread [Re: Panny] 2
#27662295 - 02/17/22 08:38 AM (1 year, 11 months ago) |
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Finally my pan cyan showing some grow. Any advice on these ones guys? I know I am not supposed to mist the fruits directly and should increase FAE (I have opened all holes - one big on upper side and 4-5 small ones on lower - I have it on all sides of the tub).
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coversall
ุฅููู ุดูุงุกู ูฑููููู



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Re: Official Copelandia/Panaeolus Thread [Re: Panny] 1
#27662306 - 02/17/22 08:50 AM (1 year, 11 months ago) |
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Quote:
Panny said: Yeah, I bet that's it.
Current recipe: 300ml bottled water 6g agar 2g instant mashed potato flakes 1.5g light malt extract
What's the reason for running potato flakes and LME? Maybe try a really simple, uncoloured mix. 300ml water//6g agar//5-6g LME?
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: shr00mFer]
#27662307 - 02/17/22 08:50 AM (1 year, 11 months ago) |
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Baba is the resident tub growth expert so listen to him first, but my advice would be to get as much FAE as you can without sacrificing humidity. I know that sounds impossible since more FAE in a tub grow always means less humidity - but what I'm really trying to say is, try to keep the humidity really high in the tub (like 90%) - don't let it go below that for the sake of more FAE, if adding more FAE will cause humidity levels to drop below that. Looking at your photo, things look quite moist in there, and you are getting enough FAE and surface evaporation to get pins formed, so that being the case, I would leave the tub set to however it was set when the pins formed.
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shr00mFer
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27662360 - 02/17/22 09:29 AM (1 year, 11 months ago) |
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Quote:
Hindsight said: Baba is the resident tub growth expert so listen to him first, but my advice would be to get as much FAE as you can without sacrificing humidity. I know that sounds impossible since more FAE in a tub grow always means less humidity - but what I'm really trying to say is, try to keep the humidity really high in the tub (like 90%) - don't let it go below that for the sake of more FAE, if adding more FAE will cause humidity levels to drop below that. Looking at your photo, things look quite moist in there, and you are getting enough FAE and surface evaporation to get pins formed, so that being the case, I would leave the tub set to however it was set when the pins formed.
Thanks. I think I have just misted it. I mist 2 or 3 times a day, swinging/faning with lid to disperse co2 after it. Hope its enough. Can I mist fruit? I do it from far with fine mister. Hopefully it will be ok for fruits.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: shr00mFer] 1
#27662371 - 02/17/22 09:35 AM (1 year, 11 months ago) |
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Misting fruits is ok - it won't negatively impact them (as people used to believe), but if you have your tub/tent set properly, you shouldn't need to. But again, I'm not a tub guy so Baba is your best bet there.
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ekabpils
Exploring Exotics



Registered: 10/20/21
Posts: 167
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27662399 - 02/17/22 10:02 AM (1 year, 11 months ago) |
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Very nice looking mycelium of NecD clone - still needs to be tested with real grow...
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shr00mFer
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27662436 - 02/17/22 10:37 AM (1 year, 11 months ago) |
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Quote:
Hindsight said: Misting fruits is ok - it won't negatively impact them (as people used to believe), but if you have your tub/tent set properly, you shouldn't need to. But again, I'm not a tub guy so Baba is your best bet there.
I put three pancake trays in a tub (sort of like fruiting chamber). On the bottom of the tub there is some perlite. Hopefully it is enough. Just a few fruits for now on 2 of the 3.
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
#27662442 - 02/17/22 10:41 AM (1 year, 11 months ago) |
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Quote:
ekabpils said: Very nice looking mycelium of NecD clone - still needs to be tested with real grow...

That's a very good start. Welcome!
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KingCloyster
Forest Mushroom ๐



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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27662601 - 02/17/22 12:58 PM (1 year, 11 months ago) |
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Does anyone have any advice for placing four 6qt shoeboxes in a sgfc. My hpoo sub is 1.75โ, lined and I plan to:
1. Wait to place sub in the sgfc until pins just start to form in shoeboxes.
2. Fill sgfc bottom with 3โ wet perlite then take the liner and sub and place the lined bottom directly on top of the perlite.
3. Mist a few times a day or keep an automated humidistat and humidifier running until harvest
Any input on additions or modifications to this plan would help.
Edited by KingCloyster (02/17/22 01:05 PM)
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Moopers
Pan noob



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Re: Official Copelandia/Panaeolus Thread [Re: KingCloyster]
#27662688 - 02/17/22 01:51 PM (1 year, 11 months ago) |
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How long are pan prints good for, on average? I am about to attempt pans again after a hiatus. I have some prints from 2020. Should I use them now to make sure I can keep the genetics going? They're P. bisporus, NecD, PHV, P. cyan 'Goliath'. Got all of them from AR.
If anyone has any recs of which of them to put to agar first, I'd appreciate it.
If they're no good, I will go back to Jambo or Estero.
I finally got the JCM chamber to work well on a recent tamp grow, so I'm really keen on getting pans moving again!
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Baba Yaga
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Registered: 09/13/20
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Re: Official Copelandia/Panaeolus Thread [Re: shr00mFer]
#27662715 - 02/17/22 02:07 PM (1 year, 11 months ago) |
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Quote:
shr00mFer said: Finally my pan cyan showing some grow. Any advice on these ones guys? I know I am not supposed to mist the fruits directly and should increase FAE (I have opened all holes - one big on upper side and 4-5 small ones on lower - I have it on all sides of the tub).

Can you show a photo of your FC showing the whole thing incl. the hole set up?
Bottom holes completely open sounds fine but I would put 1 to 2 layers of micropore tape over the top holes, to restrict air flow at the top. That way you will reduce the chimney effect and have a reservoir of moist air hovering above your substrate.
Your surface moisture and caps are looking good, moist enough to allow misting by the sound of it, if they wouldn't like it then you'd know within a day.
Indirect misting once a day and no fanning to start with seems like a good middle ground. Then watch your surface and cap moisture for a while and adjust accordingly. I'm not doing trays on perlite but fully subbed monos and usually I mist for the first 3 days of fruiting and leave it be for a few days before giving more and it works for my situation but YMMV.
Don't worry about CO2 accumulating in your tub, FAE is taking care of it.
Have a look at the first link in my sig which is how I do tubs atm.
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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: Moopers]
#27662754 - 02/17/22 02:36 PM (1 year, 11 months ago) |
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Quote:
Moopers said: How long are pan prints good for, on average? I am about to attempt pans again after a hiatus. I have some prints from 2020. Should I use them now to make sure I can keep the genetics going? They're P. bisporus, NecD, PHV, P. cyan 'Goliath'. Got all of them from AR.
If anyone has any recs of which of them to put to agar first, I'd appreciate it.
If they're no good, I will go back to Jambo or Estero.
I finally got the JCM chamber to work well on a recent tamp grow, so I'm really keen on getting pans moving again!
Why wait? The logner you wait the worse. Place them on agar today, worry about them later once you have good mycelium. Although I doubt you'll have good mycelium from 2y.o. spores.
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sloug
Strangler

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27662757 - 02/17/22 02:38 PM (1 year, 11 months ago) |
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hell yea thanks for the responses hindsight and mycosmith! ill just keep my chamber dialed in and give it some more time.
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razero



Registered: 12/23/08
Posts: 96
Loc: Jamaica
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Re: Official Copelandia/Panaeolus Thread [Re: sloug] 1
#27662876 - 02/17/22 04:19 PM (1 year, 11 months ago) |
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a
Edited by razero (06/19/23 03:40 PM)
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rockyfungus
dirty


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Re: Official Copelandia/Panaeolus Thread [Re: razero]
#27662911 - 02/17/22 05:02 PM (1 year, 11 months ago) |
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Getting sick of agar to grain failing. Last of this batch of grain. Decided to go LC
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