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OfflineMr.Giggles
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
    #27649482 - 02/07/22 12:26 PM (1 year, 11 months ago)

Quote:

THE MYCOSMITH said:
Hey psilo
Thanks for you answer and for making that thread to share you Eksperiment. You jambos look to be finish in a day or to If that trich holds back. And with you low expectations i dont think you Will be Disappointed.

Modular mimd
When i said dead, totally dry for a month or 2 is good Enough for me. What have you put on agar.?

And again Mr giggles what are that to half dead body's you try to wake alive.? Is it pan. Cint. Like on you pic above.?

Grim11311
How old is that transfer.? If it is New ther Will not be popping New growh up All over the Petri dish but it Will grow out from the transfor. I Will not clean it up i Will go back to the old agar plate. But that allso look comtamt to me. Try to take a very littel peace of pure mycelium and transfer to New dish. Do that 5-10 times If you got a flowhood and antibiotic agar. If you dont have a floowhood or antibioticagar do it 20 times and Hope you Will end up with atleast 1 clean.




Those 2 dried up pans in the pic. I collected them this past summer, I think in July or August. I'm not sure if they're cintulus, olivaceus, or fimicola. I'm pretty sure I've got a mixed bag, a couple 1 gallon sized ones lol those 2 dried up ones were too dry to get a proper tissue sample from the inside of the stipe. I ended up breaking up pieces of the stem and cap/gills. Some of them I tossed due to contam but it's starting to grow from both the dishes with spores and stipes. I'm going to transfer some of the growth to water agar and agar made with a hint of grainwater and activated charcoal. I'll post pics in a bit.


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OfflineMr.Giggles
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles]
    #27649518 - 02/07/22 01:11 PM (1 year, 11 months ago)

I think this was the pan on the left in the pic. I don't think I have a pic of the one on the right when it was fresh.



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Offlinebongoman
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles]
    #27649737 - 02/07/22 04:27 PM (1 year, 11 months ago)

Thoughts on fresh vs dried pans in terms of the psychedelic experience? To what extent does drying panaeolus fruits degrade the experience?


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Invisiblerazero
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles] * 1
    #27649749 - 02/07/22 04:38 PM (1 year, 11 months ago)

a


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He who does not research has nothing to teach.


Edited by razero (06/19/23 03:40 PM)


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: bongoman] * 1
    #27649815 - 02/07/22 05:27 PM (1 year, 11 months ago)

Quote:

bongoman said:
Thoughts on fresh vs dried pans in terms of the psychedelic experience? To what extent does drying panaeolus fruits degrade the experience?




Some say that they are much stronger when fresh, but I have not experienced this. The same goes for cubes, back when I still ate them. (Accounting for the fresh to dry weight.)

I've weighed a bunch of fruits fresh, and then again when dry, and they are pretty consistent in terms of fresh vs. dry weight. My cyans are coming in around 6.5% of fresh weight, so around 93.5% water.

Having established that the fresh to dry weight is fairly consistent from batch to batch, dry fruits are more active for me compared to an equivalent weight of fresh fruits. This doesn't make sense on paper, considering the psilocin degrades with drying, so I don't know why this is.

I have definitely found some fruits to be a little stronger or weaker, and I know that is to be expected. The differences in day to day experience could definitely be chalked up to genetics of the fruits, and/or changes in my body, amount of food in stomach, etc.

I've taken fresh fruits enough times that they always feel weaker and come on slower compared to an equivalent dry amount. They do taste really good when fresh, so I sometimes opt for fresh. I microdose, (more like threshold to threshold+ dose), 4 to 5 days a week, so most of my perspective comes from this habit.

My first pan macrodose was 9 grams fresh, (which would have dried down to 0.6 grams dry), and while it was fun, it left me wanting more and ended sooner than I wanted it to. My next macrodose will be my largest pan fruit yet, 0.7 grams dry, so I will try to compare and contrast the experience.

I think that establishing a threshold dose might be one of the most accurate methods to determine how potent a species/variety/strain/culture of mushroom is. I think that once someone goes past a threshold dose, it becomes more subjective and difficult to rate the potency of a mushroom.

(When I first started MDing there were some days that I accidentally ended up with a minidose, but after around a month the effects changed a lot. I have just enough tolerance built up that I get really energetic from a threshold dose, almost like adderall but without the speedy high. I no longer accidentally minidose myself, even though I'm taking the same amount or a touch more. Minidoses are also more comfortable and enjoyable than when I first started, as I don't feel like I'm shutting down temporarily. I am really looking forward to my next trip to see if and how my experience might change other than changes in tolerance.)


Quote:

razero said:
Cloned a 5.3g pan trop monster today and realized that I probably harvested my first set of trays too early.




I did that at first too! I was sad because none of my caps were developing how they should, but then I decided to see what happens if I let them go longer. So far, I haven't experienced a loss of potency with letting them get as big as they can.


Edited by ruawakeyet (02/07/22 05:31 PM)


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OfflinePanny
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #27649838 - 02/07/22 05:45 PM (1 year, 11 months ago)

Quote:

PsiloPsychIn said:
Panny
I’m very new to this area of cultivation so definitely get a second opinion …
I’d select from your 7:30-8 area of that plate. It looks whispy, faster than the other areas, and it is a pretty clear organized sector.
My second choice would be 11-1 , that has similar qualities.




:bow2: thank you


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Panny]
    #27650109 - 02/07/22 09:29 PM (1 year, 11 months ago)

I don’t know I personally think fresh definitely hits harder but when I go for that I also eat a good bit of fresh because if I’m gonna do it I’m gonna do it but I do the same with dried and when I do it with fresh it’s a taller ride but I do get nauseous a bit but They are plenty strong dried I’ve only really done it because I’ve eaten them so many times and I was curious but I’m never disappointed with 4 triple O capsules of dried fruit


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #27650563 - 02/08/22 08:17 AM (1 year, 11 months ago)

Quote:

ruawakeyet said:
I think that establishing a threshold dose might be one of the most accurate methods to determine how potent a species/variety/strain/culture of mushroom is. I think that once someone goes past a threshold dose, it becomes more subjective and difficult to rate the potency of a mushroom.




I absolutely agree with this and it's pretty much the method I've been using.


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27650812 - 02/08/22 11:45 AM (1 year, 11 months ago)

It's a few weeks late, but congrats on the TC status! I just saw it a minute ago.

I would like to hear anything you have to add to the threshold evaluation method.


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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #27650818 - 02/08/22 11:56 AM (1 year, 11 months ago)

Thanks. I don't have that much to add because I rarely take threshold doses, except the fact that fruit maturity still needs to be taken into account. Because when taking threshold doses, you're comparing (often) single mushrooms, when you take a full dose there is more specimens so the potency averages out a lot more. So ideally you would want to compare fruits of similar maturity, which can be incredibly hard, if not impossible to do with some species.


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #27650836 - 02/08/22 12:11 PM (1 year, 11 months ago)

I completely agree.

Last week, I ground 3.5 grams to a fine powder in a coffee grinder, then made a bunch of small chocolates and added a very small scoop of powder to each one. (1/64th teaspoon) That ended up being too weak, so while it was a more homogenized dose, I had to eat too many chocolates.

Once I get another bag of chocolate chips, I will repeat the process but using a 1/32nd teaspoon measuring spoon. I hope I can get these dialed in, because I want a good way to homogenize the dose.


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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #27650887 - 02/08/22 12:53 PM (1 year, 11 months ago)

Yes homogenizing the dose is the only remotely scientific way of getting to know thresholds for various species.


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OfflineGrim11311
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27651031 - 02/08/22 02:22 PM (1 year, 11 months ago)

Started over with this recipe for agar and made this plate. Agar recipe 30% potato water and 70% bottled water with total volume of 700ml. 7g agar powder and 2g honey and some food colouring. This was done by using swab on spore print to agar on 03/02/22.8 days growing. I see some contam... The yellow spots. Do you guys with experience in agar see any good mycelium I can transfer for T1?



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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Grim11311]
    #27651056 - 02/08/22 02:44 PM (1 year, 11 months ago)

That is quite a lot of contam but the growth left from the center looks OK to transfer.
I would pick from the mycelium that is fanning out downwards.

On a side note, with this style of container I would put the hole on
the side of the cup part, make the hole 3mm and place it close to the
rim of the cup so you can still comfortably put MP tape over the hole.


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OfflineGrim11311
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27651075 - 02/08/22 02:57 PM (1 year, 11 months ago)

Quote:

Baba Yaga said:
That is quite a lot of contam but the growth left from the center looks OK to transfer.
I would pick from the mycelium that is fanning out downwards.

On a side note, with this style of container I would put the hole on
the side of the cup part, make the hole 3mm and place it close to the
rim of the cup so you can still comfortably put MP tape over the hole.





Thanks for the reply bro. Will take your advise and make my containers like that. Will probably transfer tomorrow. Dont want the contam to get hold of the mycelium.


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: Grim11311]
    #27651641 - 02/08/22 11:12 PM (1 year, 11 months ago)

Hey grim11311
Remember to do 5-10 new dishes of transfors and Hope to get a clean one. I Can strongly recommend agar with antibiotics it Will make it a lot Easier for you.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #27652189 - 02/09/22 11:34 AM (1 year, 11 months ago)

RE homogenizing - I process every tray of pans the same way now: Dry it, dump them into a coffee bean grinder, grind into powder and load into 00 sized gel caps using a capsule loader. You get exactly 1/3 of a gram into that size capsule (assuming you pack them well using the tool), and you get the whole tray blended together instead of single fruits. I then seal 20 capsules with a vacuum sealer into a vacuum bag (cut down to a small size) with a desiccant packet in each, label the packet with the exact culture number and then use it myself or give to a friend.

I want to get smaller capsule sizes though because 0.3g of good pans is above threshold for me.


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27652245 - 02/09/22 11:59 AM (1 year, 11 months ago)

Quote:

Hindsight said:
RE homogenizing - I process every tray of pans the same way now: Dry it, dump them into a coffee bean grinder, grind into powder and load into 00 sized gel caps using a capsule loader. You get exactly 1/3 of a gram into that size capsule (assuming you pack them well using the tool), and you get the whole tray blended together instead of single fruits. I then seal 20 capsules with a vacuum sealer into a vacuum bag (cut down to a small size) with a desiccant packet in each, label the packet with the exact culture number and then use it myself or give to a friend.

I want to get smaller capsule sizes though because 0.3g of good pans is above threshold for me.




I really like that entire protocol. I'm looking into doing something like this as well.

0.3 g is WAY above threshold for me! My threshold dose is somewhere around 0.05 g!

I got a bag of single 0 capsules, and I have only made a couple by hand, but I'm getting around a half gram per capsule. Maybe I'm over packing them since I'm not using a capsule filler?

I've considered buying some Lion's Mane and grinding it to powder. Then combine the LM with a Pan micro into a capsule. I've read niacin is another good addition.


Edited by ruawakeyet (02/09/22 12:02 PM)


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #27652250 - 02/09/22 12:04 PM (1 year, 11 months ago)

Yeah I would say 0.04ish is around threshold for me too - that’s the dose I take as a microdose. I could probably go even lower than that and still feel it.

It’s not pans but I sampled my first tamps the other day. I took a full capsule (0.3g)  and barely felt them, but it was a really bad first test because I was at a windy beach away from home with many new sights and sounds and sensations - the more of those you have, the harder it makes to really feel lower doses of actives. I need to try it when I get back home.


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27652300 - 02/09/22 12:38 PM (1 year, 11 months ago)

I haven't been following the Stone Producers Thread much, but I started reading it from the beginning a few months ago. Nice haul Hindsight! I'm excited to grow these as well, I've read some good things about combining Pans with Tamps. I'll post a question for you on that thread...


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