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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
Loc: संसार
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#27642593 - 02/02/22 02:54 PM (1 year, 11 months ago) |
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Quote:
Baba Yaga said: Not sure which write up you have found, I did a few tests with mixed results but Fahtster does it on a regular basis.
https://www.shroomery.org/forums/showflat.php/Number/6886717#6886717 https://www.shroomery.org/forums/showflat.php/Number/27562340#27562340
OK I'm am officially retiring from Pan cult for the next 6-7 month. Thought about doing another run as temps are still good but got too much work on and have to go out of town on a regular basis.
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ModularMind
M.P.F.



Registered: 02/09/10
Posts: 7,902
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles]
#27642667 - 02/02/22 04:04 PM (1 year, 11 months ago) |
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Quote:
Mr.Giggles said: I haven't been doing agar very long but… I might just break some up over the agar and see if anything starts growing if I can't get a clean sample.
Try lightly tapping a cap over a dish and start from spores. Maybe use a sterilized tool to lightly dislodge spores over another dish. Then see if you could put interior material on the next plate. Finally, pulverize the entire dried fruiting body over the last and report back.
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PsiloPsychIn
PsiloPsychIn



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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] 1
#27642916 - 02/02/22 06:46 PM (1 year, 11 months ago) |
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Quote:
THE MYCOSMITH said: Yes i belive the only way you can wake a fully out dry mushroom is from sporres.but i Will not exclude that im just are stupid.
I have only done it with oyster mushroom mycelium but I resurrected mycelium dried to the consistency of a brittle potato chip on agar out. I have a thread somewhere…
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26290777&page=3&vc=1#26290777
My efforts to do the same with dried Cubensis mycelium was not as successful- but I think that was complicated by bacteria.
Somewhere on the Shroomery is a detailed tech for reviving dried mycelium from a soak in sterile water then transfer to agar.
This is not that tek - but down toward the bottom of the first page here RR himself states he has brought dried mycelium back to life many times:
https://www.shroomery.org/forums/showflat.php/Number/2390329/fpart/all/vc/1
By no means a reliable or easy method and may be very species dependent
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
Edited by PsiloPsychIn (02/02/22 07:09 PM)
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ModularMind
M.P.F.



Registered: 02/09/10
Posts: 7,902
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
#27642943 - 02/02/22 07:08 PM (1 year, 11 months ago) |
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Calls into question ideas of death and resurrection, no?
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Mr.Giggles
Time bandit



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Re: Official Copelandia/Panaeolus Thread [Re: ModularMind] 1
#27643149 - 02/02/22 09:59 PM (1 year, 11 months ago) |
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I think the mycelium just goes dormant. The dried samples I've used on agar so far usually grow fuzz all over within a few days so I think it's the mycelium reviving. I think I remember hearing somewhere that a honey mushroom rhizomorph, the bootlace as it's referred, was once revived after being dried up for like 60 years. And that humongous honey fungus is estimated to be at least 1500 yrs old, up to 11,000 when the glaciers disappeared.
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ModularMind
M.P.F.



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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles]
#27643177 - 02/02/22 10:21 PM (1 year, 11 months ago) |
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I got a clone on agar now. We’ll see who sees tomorrow.
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PsiloPsychIn
PsiloPsychIn



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Re: Official Copelandia/Panaeolus Thread [Re: ModularMind] 5
#27643256 - 02/03/22 12:08 AM (1 year, 11 months ago) |
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Second flush of the Bunnell coming in nicely…

My “Jambos” are still struggling. I think I spotted more trich - I suspect they are battling it out in the substrate but I’m hoping for even 5 grams or so dried salvage fruits
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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THE MYCOSMITH
Weekend mycologist


Registered: 01/24/22
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
#27643899 - 02/03/22 12:24 PM (1 year, 11 months ago) |
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Hey psilo Thanks for you answer and for making that thread to share you Eksperiment. You jambos look to be finish in a day or to If that trich holds back. And with you low expectations i dont think you Will be Disappointed. Modular mimd When i said dead, totally dry for a month or 2 is good Enough for me. What have you put on agar.?
And again Mr giggles what are that to half dead body's you try to wake alive.? Is it pan. Cint. Like on you pic above.?
Grim11311 How old is that transfer.? If it is New ther Will not be popping New growh up All over the Petri dish but it Will grow out from the transfor. I Will not clean it up i Will go back to the old agar plate. But that allso look comtamt to me. Try to take a very littel peace of pure mycelium and transfer to New dish. Do that 5-10 times If you got a flowhood and antibiotic agar. If you dont have a floowhood or antibioticagar do it 20 times and Hope you Will end up with atleast 1 clean.
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Grim11311
Supporter


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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
#27644072 - 02/03/22 02:36 PM (1 year, 11 months ago) |
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Quote:
THE MYCOSMITH said: Hey psilo Thanks for you answer and for making that thread to share you Eksperiment. You jambos look to be finish in a day or to If that trich holds back. And with you low expectations i dont think you Will be Disappointed. Modular mimd When i said dead, totally dry for a month or 2 is good Enough for me. What have you put on agar.?
And again Mr giggles what are that to half dead body's you try to wake alive.? Is it pan. Cint. Like on you pic above.?
Grim11311 How old is that transfer.? If it is New ther Will not be popping New growh up All over the Petri dish but it Will grow out from the transfor. I Will not clean it up i Will go back to the old agar plate. But that allso look comtamt to me. Try to take a very littel peace of pure mycelium and transfer to New dish. Do that 5-10 times If you got a flowhood and antibiotic agar. If you dont have a floowhood or antibioticagar do it 20 times and Hope you Will end up with atleast 1 clean.
Yes... I guess i should just start over... Begin from spore to agar and ask for help transferring clean mycelium.
What agar repcipe do you guys recommend using these material... Bottled water, honey, maple syrup, wbs grain water or potato water and agar... What percentage or measurements of what?
And also i cant find peat moss anywhere near me and customs dont allow it into country... What else can i use? I have coco coir, perlite, vermiculite, palm peat... Nothing else
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Baba Yaga
♥ coir grower

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Re: Official Copelandia/Panaeolus Thread [Re: Grim11311] 1
#27644207 - 02/03/22 04:15 PM (1 year, 11 months ago) |
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Use what hindsight suggested:
potato or wbs boiling water diluted with water 1:3 and agar as needed. When you use potato water then you can add a little bit of maple syrup, like 1g per agar load.
If you can't find peat moss then try 100% vermiculite which worked just fine when I used it or try using it with coir 50/50. It's not a deal breaker if you can't find peat moss.
I think palm peat and coco coir is the same thing.
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PsiloPsychIn
PsiloPsychIn



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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 5
#27644677 - 02/03/22 10:56 PM (1 year, 11 months ago) |
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The Bunnell second flush did ok. Some bigger fruits Pretty solid flush with a bald spot or two. Considering I cut some trich out of this at the start I’m happy. Very happy I may even get a third flush. Adas should I eat .5 gram dry to test the waters?
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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PsiloPsychIn
PsiloPsychIn



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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
#27644752 - 02/04/22 12:12 AM (1 year, 11 months ago) |
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MycoSmith I wish I could share your optimism about the Jambos. I’m beginning to feel more certain that what happened to them and to the wild coast - now pushing up another twisted whacky forest…
I accidentally sprayed (not once but twice!) spinosad solution on them. I had a sprayer with that in it (looks like water) because I was fighting thrips on some cannabis. Spinosad is a bacteria that does a number on bugs but doesn’t hurt plants. I’ve also read that it can be used as an organic pesticide at some points in mushroom cultivation… however I’ve also done a fair bit of reading of scientific studies of primordia formation and it turns out the bacteria in the casing soil can affect that. I figure the flood of spinosad bacteria messed up the primordia formation. In the case of the wild coast they are coming up but all misformed. I. The case of the Jambos- I’ve had a few on the very edges come up looking pretty normal but the rest are groups of very short fat and a bit twisted pins. Maybe after I get my few salvage fruits out of this flush, I’ll scrape off the casing, rinse and put a fresh casing on - hold the spinosad! 🤣
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 1
#27644867 - 02/04/22 02:11 AM (1 year, 11 months ago) |
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Nocd up 5 wild coast red spore LCs yesterday

Pretty excited as the myc looked nicer than the last time I tried this print.
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PsiloPsychIn
PsiloPsychIn



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Re: Official Copelandia/Panaeolus Thread [Re: coversall] 2
#27644924 - 02/04/22 03:51 AM (1 year, 11 months ago) |
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About a year or so ago in this thread someone said that combining Tamps and Pans in a trip was their favorite... Who was that?
I'm kind of thinking of trying it and I could not recall if it was just a 50 50 split or some other ratio.
So many flavors...
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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angam
Ad astra per stercoris...

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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 5
#27644950 - 02/04/22 04:44 AM (1 year, 11 months ago) |
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Quote:
PsiloPsychIn said: About a year or so ago in this thread someone said that combining Tamps and Pans in a trip was their favorite... Who was that?
I'm kind of thinking of trying it and I could not recall if it was just a 50 50 split or some other ratio.
So many flavors...

Mix Pan and Tampanensis the ratio of 1:3 is very interesting.I've been doing this for a year. Pan accelerates you to the first cosmic speed, and Tamp supports you with its radiance in orbit.
Edited by angam (02/04/22 04:46 AM)
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: angam]
#27645065 - 02/04/22 07:33 AM (1 year, 11 months ago) |
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I want to try that mix as a microdose as well! I heard they have a stimulant type effect. Tamps are going to be in my next spore print purchase for sure!
Psilo, nice job on the Bunnells. I love the look of the caps on those, I can't wait for warmer weather to give the Peace River Floridas a run in real conditions. I hope they exhibit the same dimpled appearance.
Edited by ruawakeyet (02/04/22 07:56 AM)
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Panny
Voyager



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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#27645368 - 02/04/22 12:03 PM (1 year, 11 months ago) |
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Wild bird seed should work for Pans right? I tried using WBS once and the mycelium stalled. There was no moisture outside of the grains, so maybe it was too dry?
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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
#27645372 - 02/04/22 12:06 PM (1 year, 11 months ago) |
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Quote:
PsiloPsychIn said: The Bunnell second flush did ok. Some bigger fruits Pretty solid flush with a bald spot or two. Considering I cut some trich out of this at the start I’m happy. Very happy I may even get a third flush. Adas should I eat .5 gram dry to test the waters?

Beautiful mate! This flush looks exactly like they do for me! Nah, go for a full gram.
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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] 2
#27645375 - 02/04/22 12:08 PM (1 year, 11 months ago) |
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Quote:
PsiloPsychIn said: About a year or so ago in this thread someone said that combining Tamps and Pans in a trip was their favorite... Who was that?
I'm kind of thinking of trying it and I could not recall if it was just a 50 50 split or some other ratio.
So many flavors...

A couple of people love it, but I am one of them. Certainly one of the best combos out there, haha. I like 1g Tamp + 0.5g Pan
Edited by Adas (02/04/22 12:08 PM)
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Panny]
#27645525 - 02/04/22 01:33 PM (1 year, 11 months ago) |
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Quote:
Panny said: Wild bird seed should work for Pans right? I tried using WBS once and the mycelium stalled. There was no moisture outside of the grains, so maybe it was too dry?
That's what I use, but I am OCD about removing the black sunflower seeds, and anything that floats after several hours of soaking gets drained off. You could be correct about the moisture level, my first WBS runs were too dry. I now prefer a 36 hour soak in cold water. I've soaked for as long as 48 hours, but it didn't make much difference. Maybe there were more burst grains when I soaked for 48, but they still worked.
I drain and rinse until the water is clear, and do this several times during the soak. If anyone has sprouted seeds for eating or juicing, treat the grains as if you were going to sprout them.
Once the soak time is up, I strain and drain any excess water, but I don't get the grain dry before loading into jars. It's still wet and sticks to your hand, the canning funnel, measuring cups, everything. Then I sterilize for 60 minutes at 15 psi, with a slow warm up.
This has been providing the ideal moisture level for me. Hope this helps and good luck!
Edited by ruawakeyet (02/04/22 01:35 PM)
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