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Invisibleruawakeyet
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Registered: 05/03/21
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #27642149 - 02/02/22 09:50 AM (1 year, 11 months ago)

Quote:

Baba Yaga said:
How successful are you with keeping a good culture long term?




Not very! :smile: I just looked back at my pictures to see when I took the clone, it was about 8 months ago. Every time I took it to grain, I also transferred to a new plate to use as inoculant for the next round. I was also pretty bad about letting the culture set on agar for too long between transfers, but that extended the time a lot. This last round really suffered in terms of yield, but it was worthwhile for around 6 months.

When I start back up in the spring, I will be trying LC again, and I also plan on trying to use G2G, both as methods to extend a culture's life expectancy.

I think some genetic information is getting lost every time a small segment is transferred to agar, so I'm hoping transferring more mycelium will keep it intact longer.


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: Drboomer]
    #27642151 - 02/02/22 09:57 AM (1 year, 11 months ago)

Hey Mr giggles

You Can not clone a dry fruit. If you relly want som out of it you Can try to take som of the sporre under the cap and put on agar.  Here Less is more...!


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles]
    #27642156 - 02/02/22 09:59 AM (1 year, 11 months ago)

Quote:

Mr.Giggles said:
Noob question: which part of these pans should I take a tissue sample from to clone on agar? I think they're cinctulus or olivaceus.




I think your best bet is in the middle of the fattest part of the stem, under the cap. Tear the stem in half on the orange line, that's the tissue you want. It looks pretty dry for cloning, but there's a chance it will work. The fruit on the left looks too dry.



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InvisibleE_Dubbya
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #27642158 - 02/02/22 10:00 AM (1 year, 11 months ago)

Quote:

THE MYCOSMITH said:
Hey Mr giggles

You Can not clone a dry fruit. If you relly want som out of it you Can try to take som of the sporre under the cap and put on agar.  Here Less is more...!




It's been done before, not the easiest thing to do but not impossible


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Invisiblecambocyan

Registered: 06/24/20
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Re: Official Copelandia/Panaeolus Thread [Re: E_Dubbya]
    #27642163 - 02/02/22 10:06 AM (1 year, 11 months ago)

yes sir ive been enjoying this genetic very much I appreciate you :mushroom2:


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
    #27642176 - 02/02/22 10:17 AM (1 year, 11 months ago)

Hey E dubbys

I find it very Hard to belive that you Can clone dry tissur/myc.


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #27642185 - 02/02/22 10:27 AM (1 year, 11 months ago)

Hey ruawakeyet

I have bin running the same pan trop. Cultur for 14 months G2G by GLC tec. And have the same cambodian cube cultur the same way for almost 10 years. Seams to be fine


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InvisibleE_Dubbya
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
    #27642187 - 02/02/22 10:27 AM (1 year, 11 months ago)

Quote:

THE MYCOSMITH said:
Hey E dubbys

I find it very Hard to belive that you Can clone dry tissur/myc.




Like I said, it has been done on here. One of the theories is that it's actually dried spores on the tissue that are growing and not the clone, but who knows for sure.


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: E_Dubbya]
    #27642212 - 02/02/22 10:41 AM (1 year, 11 months ago)

Yes i belive the only way you can wake a fully out dry mushroom is from sporres.but i Will not exclude that im just are stupid.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 3
    #27642231 - 02/02/22 10:51 AM (1 year, 11 months ago)

Dried tissue is a low cost/low tech way of storing genetics. There are a few papers on long term storage methods listing dried tissue as viable method.


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27642256 - 02/02/22 11:04 AM (1 year, 11 months ago)

Thank you baba for pointing that out:blush:
Can a hooby grower wake dead tissur up. Is it just to put it on light agar. Nevermind i have found the thread and it seams that simpel.


Edited by THE MYCOSMITH (02/02/22 11:30 AM)


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #27642391 - 02/02/22 12:08 PM (1 year, 11 months ago)

Not sure which write up you have found, I did a few tests with mixed results but Fahtster does it on a regular basis.

https://www.shroomery.org/forums/showflat.php/Number/6886717#6886717
https://www.shroomery.org/forums/showflat.php/Number/27562340#27562340

OK I'm am officially retiring from Pan cult for the next 6-7 month. Thought about doing another run as temps are still good but got too
much work on and have to go out of town on a regular basis.


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InvisibleModularMind
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #27642409 - 02/02/22 12:20 PM (1 year, 11 months ago)

Quote:

Baba Yaga said:
OK I'm am officially retiring from Pan cult for the next 6-7 month.



:nodofunderstanding:

Much success in your alternate endeavors.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: ModularMind] * 1
    #27642437 - 02/02/22 12:45 PM (1 year, 11 months ago)

Thanks brother, still got a semilanceata outdoor project going :naughty:


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27642490 - 02/02/22 01:32 PM (1 year, 11 months ago)

No i was looking at this.
https://www.shroomery.org/forums/showflat.php/Number/4360062

And RR said that it is normal by commerciel shroom farms. But I Will just Hope i never need to do it. The next Thing i put to agar is this Stones i got a friend to order for me. It is tamps and atlantis

It's gonna be in the weekend.
Baba i Hope you gonna miss you grows for i think we are many others that Will. Good luck.


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OfflineGrim11311
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
    #27642509 - 02/02/22 01:47 PM (1 year, 11 months ago)

Does any of these look like mycelium to you guys...?

The light blue agar is 30% potato water/ 70% bottled water. 1g honey, 1g maple syrup and 7.5 g agar powder. Water is 700ml



This is from older agar. Does this look like mycelium?



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OfflineMr.Giggles
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
    #27642526 - 02/02/22 02:01 PM (1 year, 11 months ago)

I haven't been doing agar very long but I think all I've done is use tissue from dried samples so far. The gym I used was dried out for awhile. I just fruited some cubes from a sample I had. I had a filthy shriveled up 2 yr old gym that I got to grow on agar, at least before I neglected it and contam took over lol. It'll be difficult to get tissue from a thin pan. I might just break some up over the agar and see if anything starts growing if I can't get a clean sample.


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: Grim11311]
    #27642533 - 02/02/22 02:05 PM (1 year, 11 months ago)

If the squar pice on pic 6 and 7 is you transfer i guss All the white spots are contamination of bad things. But If you have run a sporre swab All over it, it May be ok


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OfflineTHE MYCOSMITH
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #27642582 - 02/02/22 02:44 PM (1 year, 11 months ago)

Yes yes Mr giggles i swear i Will never doubt the raisin of a dead shroom. But tell me what are this zombies pan cint.?


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OfflineGrim11311
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
    #27642592 - 02/02/22 02:53 PM (1 year, 11 months ago)

Quote:

THE MYCOSMITH said:
If the squar pice on pic 6 and 7 is you transfer i guss All the white spots are contamination of bad things. But If you have run a sporre swab All over it, it May be ok[/quote


Any suggestions on how to clean it up or where to transfer from?

No from agar wedges


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Edited by Grim11311 (02/02/22 02:54 PM)


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