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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#27619683 - 01/15/22 10:25 AM (2 years, 14 days ago) |
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Ozone kills everything so yes, it's harmful to fungus.
Good luck Psilo! Cant wait for the results!
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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 5
#27619866 - 01/15/22 01:47 PM (2 years, 14 days ago) |
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Final update on the poorly recovered jars:
So just to recap I spawned these two jars which wouldn't recover growing out nice and fluffy but looked like this which often happens for me when shaking a jar that is almost done.
Two weeks after last shake:

I was throwing jars like this out at the beginning but after a few of them I questioned whether this was actually contamination as there were no signs of any contam before the shake, no mold, no creamy dense mycelium, no syrupy wet kernels or suspect looking bald spots. Yes they do recover poorly and they look wet after the shake but there is a difference to bacterial wet and condensation wet IMO. The final push to give them a go was reading a statement of member conicr saying that this happens sometimes but its no deal breaker. Now I spawn jars like this whenever I get them and have no problems. Also sitting in the jar for 2 weeks doesn't seem to make a difference. I still do throw jars out though just not when totally fine almost done jars recover poorly after a shake.
Both tubs received FAE during colonization and were put into full fruiting condition one day after full colonization. Temps plummeted for a couple of nights while fruiting and went down to 68F in the room, I've got the heater going now.
First clone C4T3 which was a day behind but turned out to be an OK tub. This is also the least productive clone out of the 6 I got going.
236g wet. 1 myco quart @ a spawn ratio of ~1:2.5

Second clone C3T3, pretty good and I used the other style tub for this which I like only downside is you can't see through the plastic.
327g wet. 1 myco quart @ a spawn ratio of ~1:3.5

If you have a jar like this and there was no sign of contamination before the shake then try spawning it. You might be surprised and I sincerely hope it will be in a good way.
Edit: update photo and weight
Edited by Baba Yaga (01/16/22 04:27 PM)
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 3
#27619876 - 01/15/22 01:52 PM (2 years, 14 days ago) |
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Looking great Baba! I know from working with Bisporus that when you shake near full colonization the recovery looks weird, but it's nothing to lose sleep for.
Speaking of Bisporus, the genetics I have from VL should finish colonizing a jar soon, I may try a monotub Bisp grow for the first time. Should B fun.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 2
#27619983 - 01/15/22 03:30 PM (2 years, 13 days ago) |
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Baba I am very surprised they worked out but also very happy for you. Adas I agree - I try to shake my pans at around 50% and not any more for that exact reason - they always look weird. APE does the same thing for me. Oh and I'm right with you on the Bispos from VL. Have a handful on grain now.
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PsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#27620187 - 01/15/22 06:31 PM (2 years, 13 days ago) |
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Quote:
Adas said: Ozone kills everything so yes, it's harmful to fungus.
Good luck Psilo! Cant wait for the results!
Thanks Adas - I have my first tray of Bunnell not far behind. Excited to see what that’s all about. 😊
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District
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27620498 - 01/16/22 01:05 AM (2 years, 13 days ago) |
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Quote:
Adas said: Here are some of my recent failures.
Bisporus tub infested by mites, which invited Trich. And a lonely pin. At least I've learned something though - this is the first Bisporus box ever that I've just cased and closed it. It seems like they don't need a lot of evaporation to start pinning, just constant conditions. But it's also just 1 pin so take it with a tablespoon of salt. It brings me closer to growing them in a monotub though.

Bunnell Cyan which refused to produce any reasonable pinset. The mother culture was my most uniform and beautiful culture at first, but after just 2 transfers turned into this.

For comparison, this was it before the 2 transfers. Makes me think it got attacked by a myco virus or something. Scary shite.

That agar looks suspect to me with that muted inner ring. Ive gotten that before. A clean pan culture will not exhibit that inner ring.
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: District]
#27620597 - 01/16/22 06:03 AM (2 years, 13 days ago) |
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That muted inner ring is completely normal to many species and cultures. There's absolutely nothing wrong with that. Something else must have happened.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 4
#27621034 - 01/16/22 11:58 AM (2 years, 13 days ago) |
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Third flush BVI on manure/coir. These are a little smaller than the last flush because I completely scraped the casing layer off due to it being so mangled after the harvest of the 1st and 2nd flush, then re-cased it. It fruited really fast after that - 3 or 4 days I think it was when I saw pins. I didn't bottom water this flush which reduced the yield and size as well. These really like to be bottom watered and I'm going to experiment more with that in all my trays.

A couple mini NecD trays, 2nd or 3rd flush, I forget which:


I have three new trays in the tent, and four outside the tent consolidating. Beyond those, I've switched to 6qt shoeboxes for these - will treat just like I do trays (and put in tent with no lid once cased/consolidated), only make the sub 3" deep per JOC. I have two right now, one coir-based and one straw-based. The odd thing is that after 3 days, I see zero growth in either of them. Normally I'm used to seeing nearly full consolidation of the surface in 3-4 days on straw/manure. I'm worried the subs are too wet. Dunno what else it could be because I prepped and pasteurized the subs per normal (overnight instapot). I'll just be patient and make my next subs a bit more dry.
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27621036 - 01/16/22 12:05 PM (2 years, 13 days ago) |
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Really nice man! Yea lack of colonization usually comes down to oxygen starvation, but if the sub is far too wet it can happen as well.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27621108 - 01/16/22 12:54 PM (2 years, 13 days ago) |
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Thanks Adas - how much oxygen are you giving during consolidation? When I was using trays, I used snap on lids with some holes. With the new shoebox style trays, I'm just setting the lid down on top and it's not air tight so figured it would be enough.
I did just realize these two tubs are a Cambo and a Tamp. One is SVM and the other CVM. Both are my first attempt from MS. I have some more tamps on straight coir too. Tamps on agar have gone really slow for me so maybe that's the issue here - expecting them to colonize as fast as pans. I have no clue how Cambo should go.
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27621128 - 01/16/22 01:05 PM (2 years, 13 days ago) |
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Anything that's not airtight should work fine. Stalling can also happen if the manure wasn't leeched properly, but I don't think that's the case with your sub.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27621143 - 01/16/22 01:18 PM (2 years, 13 days ago) |
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Yeah it can't be the manure because it's the same kind I've used with success before. I have two kinds - one so composted it looks like dirt, and another that's field aged and leached and very grassy looking, but not composted. Both seem to work ok. Need to test the compost looking one more though.
I'm excited today because I finally pulled the trigger on a flow hood. 36x24 filter and a suitable blower are on the way. Have to build the box which won't be any problem. I'm over SAB work. Too inconsistent for me. As skill increases, patience decreases, and I think I'm just moving too fast for a SAB to work properly.
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27621161 - 01/16/22 01:34 PM (2 years, 13 days ago) |
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Hmmm I don't have better idea to why they are stalling :/ Maybe you could post a pic so we can see more of what's going on?
Flowhood is the way to go! SAB work is slow and sometimes painful, haha. I wish I could upgrade. Maybe one day.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27621176 - 01/16/22 01:40 PM (2 years, 13 days ago) |
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Here is the pan cambo from MS. First attempt. To my eyes, the only thing I see wrong is maybe too wet. But people are always talking about how wet pans like it. I don't feel like this is much wetter than my previous tray grows, but is more than twice as deep, and it's tough to compare wetness because these totes have clear sides while the metal trays I've exclusively grown in up to now obviously aren't see-through so they could be just as wet. The top layer is still quite thin as I always do - just enough to keep the grains covered. Formula is 1qt verm, 2qt straw, 1qt manure. Brought to field capacity, pasteurized, aged a few days, then some water added to bring it back up to field cap again.
It could just be the culture. It went well on grain though.

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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 2
#27621192 - 01/16/22 02:02 PM (2 years, 13 days ago) |
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If your sub prep and material hasn't changed then it might be your culture, hard to tell without poking ones nose in it but something is up with this one. If it doesn't smell off then it is most likely not oxygen deprivation, I'm sure you would smell if your sub goes anaerobe and the top or top half should be colonizing at least.
I had made my first compost based sub too wet and it wouldn't colonize properly and triched out in the end but it also started smelling a bit like a MacDonalds trash bin before it went down.
Having said this, I don't see the point of going 3" deep, will this help with later flushes?
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#27621198 - 01/16/22 02:07 PM (2 years, 12 days ago) |
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Quote:
Baba Yaga said: Having said this, I don't see the point of going 3" deep, will this help with later flushes?
JOC said just a few days ago he always does 3" deep now. Common wisdom used to be 1-1.5" or so which is what I was running. I'm going to give the 3" a shot and see what happens. If it doesn't work for me I can always go back to trays. God knows I have enough of them.
RE the smell, yeah it still smells just fine.
I did change up my sub prep a bit. I am now trying boiling my chopped straw prior to mixing with manure/verm and pasteurizing. I'm doing this in an effort to clean it up, let most of the straw seeds sink to the bottom of the pot (basically a seed-straining mechanism), and to fully hydrate the straw prior to pasteurization so I'm closer to field capacity going during that process. The manure isn't boiled so the microbes from that will then mix with the straw/verm during pasteurization.
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 2
#27621208 - 01/16/22 02:15 PM (2 years, 12 days ago) |
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They like it wet, but only on the casing layer and with plenty of fae.
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LeftSideDriver
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a] 3
#27621764 - 01/16/22 10:33 PM (2 years, 12 days ago) |
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Welp, it seems I have grossly underestimated how much FAE has been happening in my Marthas but the more they've been getting, the more normal my trays look! Contemplating completely removing the poly thats in the ducting holes at the top of my Martha. This tray of Rainbow Eucalyptus is the most normal looking pan tray I've had so far and I think I am going to harvest it shortly:

Now I just need this Nec-D tray to look like that and I will happy!

The trick right now seems to be how to how to maintain something like 77º F while allowing lots of FAE. I don't want to heat the room around the tent if I can avoid it.
I have my fruiting temp at about 77º F and my pinning temp at 82º - 83ºF. Any reason to up the fruiting temp?
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moonwatcher


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Re: Official Copelandia/Panaeolus Thread [Re: LeftSideDriver] 1
#27621779 - 01/16/22 10:54 PM (2 years, 12 days ago) |
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My first ever pan pin! However, I'm not sure he's gonna make it, grows a bit too slowly (I think) and is chubby + fuzzy. Will see. Experimenting with various substrates, this is potting soil (and the casing is potting soil too).

This should be a better pinset & not just 1 lonely pin, lots of knotting, which I'm not sure is very visible on a pic:

Having lots of trouble dialing in the humidity, I'm starting to think that primordia form most readily during a very dry phase but then the actual bodies start growing fast when the casing gets soggy. Makes sense when you consider how quickly some mushrooms emerge after a rain.
Edited by moonwatcher (01/16/22 11:11 PM)
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Baba Yaga
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Quote:
LeftSideDriver said: Welp, it seems I have grossly underestimated how much FAE has been happening in my Marthas but the more they've been getting, the more normal my trays look! Contemplating completely removing the poly thats in the ducting holes at the top of my Martha. This tray of Rainbow Eucalyptus is the most normal looking pan tray I've had so far and I think I am going to harvest it shortly:

Now I just need this Nec-D tray to look like that and I will happy!

The trick right now seems to be how to how to maintain something like 77º F while allowing lots of FAE. I don't want to heat the room around the tent if I can avoid it.
I have my fruiting temp at about 77º F and my pinning temp at 82º - 83ºF. Any reason to up the fruiting temp?
I think you need more moisture in there I bet they would go nuts with wet feet.
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