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Offlinerockyfungus
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 2
    #27617312 - 01/13/22 11:51 AM (2 years, 16 days ago)

Wild coast just hit grain again. Can't wait to smell them.
About to transfer some burnells and possibly hit grain too.


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OfflineElric
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus] * 2
    #27617319 - 01/13/22 11:53 AM (2 years, 16 days ago)

My favorite part of tray recovery is waiting to smell them hahaha


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~Elric~



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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Elric] * 2
    #27617587 - 01/13/22 03:38 PM (2 years, 15 days ago)

Here are some of my recent failures.

Bisporus tub infested by mites, which invited Trich. And a lonely pin. At least I've learned something though - this is the first Bisporus box ever that I've just cased and closed it. It seems like they don't need a lot of evaporation to start pinning, just constant conditions. But it's also just 1 pin so take it with a tablespoon of salt. It brings me closer to growing them in a monotub though.


Bunnell Cyan which refused to produce any reasonable pinset. The mother culture was my most uniform and beautiful culture at first, but after just 2 transfers turned into this.


For comparison, this was it before the 2 transfers. Makes me think it got attacked by a myco virus or something. Scary shite.


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OfflineKingCloyster
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27617723 - 01/13/22 06:00 PM (2 years, 15 days ago)

Can someone help me figure out where I should be transferring from on this Spore Print>Standard MEA Agar? It all looks the same and can’t tell what is rhizomorphic or tomentose. Should I just target the fastest/thickest/healthiest growth? I have two plates pictured below


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: KingCloyster] * 3
    #27617768 - 01/13/22 07:03 PM (2 years, 15 days ago)

Left plate, it all looks great except for the over-run spot from 11:00-1:00. I would take from the leading edge of any of that. 10:00 looks to be the fastest but I'd take like 4 or 5 from different spots on that plate (except that 11-1 area).

Right plate: 3:00 is looking great, and I would also do 7:00 and 8:00. Skip the over-run area at the top of the plate.

In both plates, you're going for the growth that has outward reaching lines. There is TONS of that on both your plates so you are looking good.


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Invisiblerazero
I'm a teapot


Registered: 12/23/08
Posts: 96
Loc: Jamaica
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27618031 - 01/14/22 01:49 AM (2 years, 15 days ago)

a


--------------------
He who does not research has nothing to teach.


Edited by razero (05/27/23 04:49 AM)


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OfflineLeftSideDriver
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Re: Official Copelandia/Panaeolus Thread [Re: razero]
    #27618403 - 01/14/22 11:00 AM (2 years, 15 days ago)

Speaking of pan myc and smells, in one of my recent failures, I was spawning to bulk with what I thought was a super zippy fast pan clone and I noticed the grain smelled like alcohol or formaldehyde.  Not like fermented grain but like some kind of distillate.  Very weird.  I should have pitched it, but naturally I forged ahead and wasted a bunch of sub on it.  it colonized and starting eating the casing but never fruited.  When I pitched it, it smelled vaguely earthy and fungus like but definitely not like pans.  Thinking that i had just waited too long to spawn to bulk, I made a liquid culture to try again but it did not look right so i pitched that too.  Whats interesting is that it had the same distillate / alcohol / formaldehyde smell.

Does this sound familiar to anyone?


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Invisiblebigfootscreepyuncl
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Re: Official Copelandia/Panaeolus Thread [Re: LeftSideDriver]
    #27618417 - 01/14/22 11:07 AM (2 years, 15 days ago)

That sounds like you had a wild yeast or lactobacillus infection that actually caused fermentation (alcohol production), which then was exposed to O2 and some bacteria which caused acetic acid (vinegar/nail polish remover smell) to develop. This is just a guess based 100% off my experience brewing beer so if I'm wrong I would appreciate someone else correcting me


--------------------



I :heart: 5318008


NOT a virgin!


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: LeftSideDriver] * 1
    #27618418 - 01/14/22 11:08 AM (2 years, 15 days ago)

That sounds like Yeast. Happened to me before. While Yeast is not the biggest problem, it can set your culture back. It's an unnecesary competition after all.


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OfflineLeftSideDriver
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27619114 - 01/14/22 08:23 PM (2 years, 14 days ago)

Well I am getting closer to dialing in my martha's parameters to get these pans growing proper but I have a little ways to go it seems.  I had a dip in my temps to 74ΒΊ F so I added a heater on day 6 of fruiting and I finally got some Nec-D pins on Day 7 of fruiting conditions with a temp of 82ΒΊ F. I have another tray of Nec-D (same clone) that is 2 days behind.  The pin sets are not glorious but the one that is 2 days behind seems to have benefitted from the higher temps and the pin set is noticeably better.  They are are currently on day 9 and 7 respectively.

Day 9:



Day 7:



I am concerned that the day 9 trays will not produce much and the fruit is not growing much.  Those pins are 3 days old now. :-( In another recent mostly failed attempt, my Mezcala Jalisco pins also never really grew up and I barely managed to get a print.

Any ideas what the issue could be here? 

I have always ozonated the water in my mister reservoir and I am wondering if that is related so I shut off the ozone generator last night. 

Can a lack of FAE cause small fruits and/or aborts? 

I have a separate tent that will be held at 94%-95% RH for the rest of the fruit out and I am wondering when to move these trays over to that tent.

There is clearly a lot of potential for these trays still and I wish I could figure out the magic bullet to make them pop!

I know its not the genetics because the clones are from large really meaty looking fruit a friend grew.


Edited by LeftSideDriver (01/14/22 10:08 PM)


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: LeftSideDriver]
    #27619181 - 01/14/22 09:29 PM (2 years, 14 days ago)

I've never used nor heard of people using ozonated water. I know excess ozone is harmful to us, I don't know how fungus could be affected. I have used a little bit of hydrogen peroxide in the fogger water safely. That helps keep the fogger reservoir clean and provides a small amount of extra oxygen.

By mister do you mean fogger? Do you have a fan to exchange the air in your martha? Are you cycling the two?

Lack of FAE can cause small fruits and aborts.

The pictures aren't showing up for me. You might need to upload them again, then we can take a look.

Edit: The pictures show up now. I haven't grown Nec-D yet, but PHV is sometimes slow on the first flush. The first pins can be sluggish for a couple of days, then they get going after that.


Edited by ruawakeyet (01/14/22 10:21 PM)


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OfflineLeftSideDriver
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #27619220 - 01/14/22 10:21 PM (2 years, 14 days ago)

Quote:

ruawakeyet said:
I've never used nor heard of people using ozonated water. I know excess ozone is harmful to us, I don't know how fungus could be affected. I have used a little bit of hydrogen peroxide in the fogger water safely. That helps keep the fogger reservoir clean and provides a small amount of extra oxygen.




Yeah i got the ozone generator tip from a friend and it works just fine with cubes and might not be the culprit but I need to remove variables so its off for now.  I will try either a little peroxide or maybe even an aquarium UV filter unless thats somehow a bad idea.

Quote:


By mister do you mean fogger? Do you have a fan to exchange the air in your martha? Are you cycling the two?




Yes I meant fogger, sorry.  6 head ultrasonic fountain fogger in a box with a fan and ducting to each of the three shelves of the martha. There is input fan with a filter in front of it pushing air in the top right of the martha (it a grow tent like you would use for cannabis so it has ducting holes) and an exhaust fan at the bottom in the back.  They are coupled together and run on a cycle timer and I think its 2 mins on and 13 mins off to create a 15 min cycle.

The fogger is conncted to a controller and the range set to 90% to 98% humidity.

Quote:


Lack of FAE can cause small fruits and aborts.

The pictures aren't showing up for me. You might need to upload them again, then we can take a look.




Pics should be fixed now.  I will crank up the speed of the exhaust fan and perhgaps the duration so I can really pull the air out and provide more FAE

I am also considering putting a bin of water with some aquarium bubblers in it and essentially make it a hybrid JCM / Jartha.  I reckon this will bring in more FAE and if I can get it to provide a steady amount of humdity it might make it easy to switch it between pinning and fruiting mode


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Invisibleekabpils
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Re: Official Copelandia/Panaeolus Thread [Re: LeftSideDriver] * 7
    #27619279 - 01/15/22 12:09 AM (2 years, 14 days ago)

So this is the first wave of NectD:





250 gr


--------------------


Edited by ekabpils (01/15/22 12:10 AM)


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InvisibleBaba Yaga
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Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
    #27619288 - 01/15/22 12:19 AM (2 years, 14 days ago)

Nice trays mate!

How are you fruiting those?


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OfflineLeftSideDriver
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Re: Official Copelandia/Panaeolus Thread [Re: LeftSideDriver]
    #27619293 - 01/15/22 12:26 AM (2 years, 14 days ago)

Quote:

ruawakeyet said:

Edit: The pictures show up now. I haven't grown Nec-D yet, but PHV is sometimes slow on the first flush. The first pins can be sluggish for a couple of days, then they get going after that.




I need to get a print of that HPV!  Assuming I can figure out how to grow these pans right! lol

When would you recommend switching from "pinning mode" to "fruit out" mode, if you will.  Or are you one of the smart people that just uses a JCM and doesn't worry about that? :-) A JCMartha set it and forget it kind of thing would be cool.  The pics I see of the Jambos with 0 effort are inspiring but I like the scale of a Martha


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: LeftSideDriver] * 4
    #27619342 - 01/15/22 01:54 AM (2 years, 14 days ago)



Edited by Baba Yaga (01/15/22 02:03 AM)


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 3
    #27619367 - 01/15/22 02:43 AM (2 years, 14 days ago)

Of the 4 1/2 varieties I spawned very early in the morning on the 13th… the Jambo:



And Wild Coast:



Look to be moving the fastest. I figure they will be cased and in the fruiting camber by next weekend.
Excited to be working with Pans again.

:awedance:


--------------------
What are they saying? Listen carefully, it might be something you need to hear...





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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: LeftSideDriver]
    #27619614 - 01/15/22 09:27 AM (2 years, 14 days ago)

When would you recommend switching from "pinning mode" to "fruit out" mode, if you will.  Or are you one of the smart people that just uses a JCM and doesn't worry about that? :-) A JCMartha set it and forget it kind of thing would be cool.  The pics I see of the Jambos with 0 effort are inspiring but I like the scale of a Martha

I am using a unique setup, but it does the same thing as a Jartha, (JOC Tek). I alternate between the fog cycle and a warm air cycle inside a grow tent. I don't change the conditions for pinning to fruiting, I just place the trays in it once cased.


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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #27619620 - 01/15/22 09:33 AM (2 years, 14 days ago)

I usually give it 2-3 days after full colonization to case and fruit.


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LAGM2020


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #27619659 - 01/15/22 10:06 AM (2 years, 14 days ago)

I usually wait 1 or 2 days before casing. I haven't tried giving the trays 12 to 24 hours after casing like some do, but I will try it eventually.


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