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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 3
    #27591736 - 12/23/21 10:37 AM (2 years, 1 month ago)

Quote:

Hindsight said:
JOC what variety are those short chunky ones?



That’s the pan cam sandoze X pan trop Australia dwarf I’ve been stabilizing


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Moseph]
    #27591738 - 12/23/21 10:38 AM (2 years, 1 month ago)

I will let others chime in on the bispos - I just streaked some plates with a bispo spore print a few days ago so that's as far as I've gone with that variety.

But as for the cyan jars, the first thing I will say is that they look too wet and the grains look overhydrated to me. It can be ok like that but puts you at greater risk of bacterial contams. But as for the mycelium in the jars, they look pretty good to me. Only thing that caught my eye is the couple of spots in jar 2 that appear a bit overly dense like marshmallow cream. That can be a sign of bacterial contamination, but it's too early to tell and I wouldn't worry about it yet... wait another 5 or 7 days to see how it plays out. It's probably fine.

@JOC - nice, looking forward to when those are available. I like the different look to them.


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OfflineMoseph
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27591795 - 12/23/21 11:37 AM (2 years, 1 month ago)

Quote:

Hindsight said:
But as for the cyan jars, the first thing I will say is that they look too wet and the grains look overhydrated to me.

@JOC - nice, looking forward to when those are available. I like the different look to them.




I was thinking the same thing. Is there anyway to safely "dry out" a jar?

Maybe pop the jars in a box filled with desiccant, since it has a filter breather? Would that suck out some moisture?


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OfflineLand TroutM
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27591797 - 12/23/21 11:38 AM (2 years, 1 month ago)

Jake, for crossing pan species can you just go mono to mono?  Or is there some other kind of wizardry involved?


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Moseph]
    #27591820 - 12/23/21 12:21 PM (2 years, 1 month ago)

I wouldn't bother. They will dry over time if you have a good vent hole and SFD or other type of filter, but instead of focusing on these, just focus on your next attempts and hope these jars turn out ok, which they very well may.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 2
    #27592330 - 12/23/21 08:19 PM (2 years, 1 month ago)

So this is interesting.....

My coir/manure attempt is colonizing well, and it's easier to see the colonization than straw/manure just because coir is smoother, darker, and consistently colored. I have mostly been using disposable aluminum baking pans that come with snap-on plastic dome lids. The dome lids are nice humidity domes during colonization. I poke 4x 1/4" holes in the lids to allow some air flow, one at 12:00, 3:00, 6:00, and 9:00. If you look at the pic below, you can see the only spots with poor colonization are right in front of those holes. So the question is - why? Does the mycelium really not like the fresh air? Or maybe it doesn't like the lower humidity? The room swings between 50% and 60% RH and temps are a constant 78F. Or could the holes be serving to let airborne contamination in that is then competing with the mycelium? Top layer is just pure coir so I'm hesitant to think contamination but am open to opinion:






And here's another mystery. I used the 12-hour Instapot keep-warm pasteurization method for the coir+manure substrate in this tray and check it out - a little seed sprout. WTH? I can't imagine 12 hours at 163F not being enough to kill a seed!



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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 2
    #27592584 - 12/24/21 03:21 AM (2 years, 1 month ago)

Seeds can be hardy as fuck sometimes.

Florida Cyans ready to rock!



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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27592598 - 12/24/21 04:01 AM (2 years, 1 month ago)

At what level are you having the holes Hindsight? Put them at the top and you wont have this happening.

I have sprouts popping up after 12 hrs @ 168F for manure and I bucket tek the coir. Never knew for sure where the seeds are coming from.


Managed to spawn 6 tubs last morning, a couple were slightly borderline in terms of colonization status.


HAPPY HOLIDAYS EVERYONE


Edited by Baba Yaga (12/24/21 04:11 AM)


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Offlineangam
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 6
    #27592624 - 12/24/21 05:22 AM (2 years, 1 month ago)

Happy holidays, brothers! Some mushrooms for the mood! Nec D.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: angam]
    #27592650 - 12/24/21 06:34 AM (2 years, 1 month ago)

Looking good as always Angam.

@Baba, good to know you are getting sprouts too. I’m not really worried about it, more just shocked that any seed could survive 12 hours at 163F. As for the holes, yeah I had previously been poking holes near the top but switched to using a punch which can only get at the bottom of the lid. I’ll go back to the other method. I have been experimenting with switching from the disposable aluminum pans to reusable metal pans and those have no lids, so I’ve been using cling wrap or foil over them with some holes poked and noticed the same problem. Maybe I’ll just skip the holes completely on those.


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Invisibleelpico
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 4
    #27592884 - 12/24/21 10:48 AM (2 years, 1 month ago)

Beautiful trays, angam.  Love the red gills.

These bisporus jars keep pushing out fruits and have been surprisingly productive(ignore the old label, lol).  Took some clones for a scaled up grow.



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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: elpico] * 1
    #27593790 - 12/25/21 07:27 AM (2 years, 1 month ago)

Pan bispo coming through with the most even germination I think I've ever experienced from any mushroom variety. Usually I only get germination at the start of my streak and sometimes at the corners.



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OfflineAdrenaline_Drip
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Re: Official Copelandia/Panaeolus Thread [Re: Moseph]
    #27594589 - 12/26/21 02:21 AM (2 years, 1 month ago)

Store bought manure?  What brand do y’all recommend?


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Adrenaline_Drip] * 1
    #27594591 - 12/26/21 02:24 AM (2 years, 1 month ago)

Here’s my pre made substrate, The manure you use makes a big difference ime grass fed field aged sun bleached pile cooked cow or horse manure is optimal
https://inoculatetheworld.com/product/pan-bulk-substrate/


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Land Trout] * 2
    #27594592 - 12/26/21 02:28 AM (2 years, 1 month ago)

Quote:

Land Trout said:
Jake, for crossing pan species can you just go mono to mono?  Or is there some other kind of wizardry involved?



Yes I get my mono’s then attempt breeding with and without my venom reagent it’s The venom pair that usually works more often tho


--------------------
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online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
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EXOTICS
[


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] * 1
    #27595279 - 12/26/21 03:15 PM (2 years, 1 month ago)

Update on my coir/manure vs straw/manure test: The half-size trays went into the tent today, 48 hours after they had been cased. The top two trays are of one grain jar split between the two trays, and the bottom two trays are of another grain jar split between those two trays. In other words, same culture for each tray pair. The straw trays grew through their casing layer. I've never had that happen before. Any idea on the cause (cased too early?) or what I should do about it, if anything? re-case it? Leave it alone? Leaning toward the latter.

Oh and pay no mind to my nasty drip tray LOL. That's getting cleaned this week when I get some time. I do it monthly.



Edited by Hindsight (12/26/21 05:03 PM)


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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27595302 - 12/26/21 03:32 PM (2 years, 1 month ago)

Lonely Bisporus appeared a MONTH after casing. How depressing.



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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27595336 - 12/26/21 03:53 PM (2 years, 1 month ago)

So I'm thinking I'll move my P. Bisporus to "bulk" this week. I have a few questions before i do...

I can't find any vermiculite with in a 75 mile radius so I'm am going to do without. Should be fine right?

After reading @P9hu7's write up on top layer/casing I was thinking about using some bigger containers since the ones I'm using are only 3" deep. I was thinking of switching to these. Let me know what y'all think.


The smaller one with the black bottom is what I'm currently using. Also, should I put any holes at the 2 or 3 inch height to allow for CO2 to escape?


Edited by Moseph (12/26/21 03:54 PM)


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Invisiblemistamonsta
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27595390 - 12/26/21 04:27 PM (2 years, 1 month ago)

Quote:

Adas said:
Lonely Bisporus appeared a MONTH after casing. How depressing.







Well at least you have some fruiting genetics to clone :smile:


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: mistamonsta]
    #27595913 - 12/27/21 02:43 AM (2 years, 1 month ago)

Not really, I've done cloning before and it wasn't very useful. It's not genetics that's the problem, it's my techniques somehow.


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