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jcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 5
#27589239 - 12/21/21 08:40 AM (2 years, 1 month ago) |
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sup yall just wanted to pop in and wish all my fellow pan heads a merry xmas๐๐ im finally all the way done with all the estate shit from my brother and im just now able to have a lil down time and let it soak in and relax a bit. i will b on vacation for 2 weeks after tom thank god lol but after the 1st of the year il b firing sum pans back up. i hope u all are doin well๐๐
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smalltalk_canceled
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
#27589266 - 12/21/21 09:17 AM (2 years, 1 month ago) |
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This is a cube right,not a pan?
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (12/21/21 09:17 AM)
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Hindsight
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Too soon to tell
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 2
#27589305 - 12/21/21 10:10 AM (2 years, 1 month ago) |
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Thatโs a cube
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] 1
#27589306 - 12/21/21 10:11 AM (2 years, 1 month ago) |
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I bet a print
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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] 3
#27589420 - 12/21/21 12:18 PM (2 years, 1 month ago) |
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Quote:
smalltalk_canceled said: This is a cube right,not a pan?

How come you are getting repeatedly confused about what's growing in your jars?
Cube.
Quote:
Hindsight said: Mediocre first flush of a clone culture. Bigger fruits than last time I think, but not nearly as full of a flush.

They are really pretty man, the caps don't seem as light as before but looks like they carried over the low spore production.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27589458 - 12/21/21 12:47 PM (2 years, 1 month ago) |
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Thanks Baba, yeah they looked a lot darker a couple days ago but lightened up even more since then. Not QUITE as light as before but close - bit more spores which makes the gills darker. Printing them now to see if I can get visible prints off them this time.
I dunno how you guys can tell that little pin is a cube so soon. You have better eyes than me!
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Drboomer
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27589462 - 12/21/21 12:49 PM (2 years, 1 month ago) |
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Get super stoned during sab work? Mislabels have happened for that reason.
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Mycoplex
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#27589672 - 12/21/21 04:01 PM (2 years, 1 month ago) |
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Quote:
Baba Yaga said:
Quote:
Mycoplex said: That agar looks good razero. Good luck!
In another thread I wrote about difficulties I had with fruiting pans using the MacMerdin Simple Pan Cakes tek. I was about to give up on these little containers today after 7 days without any sign of pins, but I noticed some tiny pins popping out.
Admittedly I am not very proficient with pans so I know I screwed something up here, including probably over-watering these, not giving them enough FAE, probably fruiting them too early, and a combination of all of the above. I see there is overlay on the casing layer too which is another bad sign.
I live in a climate that makes fruiting from cakes really difficult which is why I almost always spawn cakes to bulk. Using a SGFC is not practical for me because of how dry the ambient air is even when accounting for manual humidification. I think most people see pins with this tek in 3 days or so but it took me over twice that long.

I wouldn't call this overlay yet.
From what I hear dry climate can be a real bummer. IDK never did much pan cakes or fruited them in a humidity chamber.
Just shooting from the hip, but I would try a couple of cakes and recase them once primordia are showing, to cover them up with something light and fluffy to keep a microclimate till they are a bit bigger, not sure about how moist to keep this additional casing, would also try different materials as well like coir or verm or a mix or stick with 50/50. That's a matter of trial and error.
A.k.a is a desert dweller IIRC maybe he can share some experience.
Earlier in the thread I was posting about issues with getting Pans to pin with these MacMerdin cakes (basically a PF-tek-type grow style for Pans).
Well I took the hint from Baba and others, and basically addressed the problem by kicking up BOTH humidity and FAE, at the same time, all the time. I basically went back to how I used to grow cubes before misting and fanning became 'old tek.' Multiple times per day I visited these things, misted the living heck out of them (almost like watering a plant), and aired them out.
Without some automated way of doing this (like a greenhouse or something) I think this species is pretty labor intensive and requires special attention. I'm glad I at least see some fruits. I also think I spawned the cakes way too early as well.
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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Mycoplex]
#27589711 - 12/21/21 04:34 PM (2 years, 1 month ago) |
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You got fruits, great!
How are you fruiting your cakes? Is this a greenhouse with a few trays in it?
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Mycoplex
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27589786 - 12/21/21 05:59 PM (2 years, 1 month ago) |
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Yup Baba that is exactly what it is. In the next iteration I have broken up the cakes into a bulk substrate and am going to case it. I hope to have better luck with that than the cakes which at this point have been in fruiting for about 10 days.
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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Mycoplex] 1
#27589820 - 12/21/21 06:24 PM (2 years, 1 month ago) |
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If you don't have anything hooked up to the greenhouse like a fogger and a couple of fans then I'm afraid that the little wet coir you having in your trays is not enough to provide ample conditions for a big volume like this and it will end up too dry, making constant misting as you are doing now absolutely necessary.
When fruiting pan cakes you should have them in an SGFC like MacMerdin or use a humidity chamber like this one by Mateja
https://www.shroomery.org/forums/showflat.php/Number/25339094#25339094
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mistamonsta
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27591090 - 12/22/21 07:12 PM (2 years, 1 month ago) |
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Hey guys, great thread! I will definitely be trying to read through all this info in the future. I've just started my first panaeolus attempt and right now I need to ask for a little guidance...
Here's my four PDA (Y)(P) plates I inoculated with pan cyan spores on the 12th
   
I'm going to be completely honest and admit I don't know what I'm looking at here, it's nothing like cubensis mycelium. Is any of this healthy? Which bits do I want to select transfers from?
Here's what the plates looked like three days ago, please forgive the light reflection on the second and third photos
  
Any help is appreciated
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Moseph
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Re: Official Copelandia/Panaeolus Thread [Re: mistamonsta]
#27591112 - 12/22/21 07:33 PM (2 years, 1 month ago) |
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Hey all. Is this where I should've posted looking for opinions on copelandia spawn jars?
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Moseph] 1
#27591134 - 12/22/21 07:53 PM (2 years, 1 month ago) |
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@Moseph, sure post them up. Let us know what specific variety you are growing.
@Mistamonsta - Yeah Pan Cyan mycelium can look different from cube mycelium but I have grown cubes that looked about the same as pans. It can get a LITTLE rhizo but it's not too common. I have found that compared to cubes, pans are very sensitive to agar formula and small changes cause big changes in growth pattern.
Having said that, it looks like you have plenty of great places to transfer from, and a few areas I would run away from. Going by your plate numbers:
1. Too much going on in the middle. Take transfers from the edges where there is uncolonized agar. So the edge of the growth at 12:00, edge of the growth at 5:00, edge of the growth at 8:00. Overall this plate looks good and typical to me. 2. 12:00, 5:00, and 7:00 is what I'd do (again on the edges) 3. The only thing I like on this plate are the top two round growths. I'd transfer from them, taking the transfer from the top away from the rest of the plate. Though the two growths at 7:30 that are joined together might be ok too. 4. I don't like any of that. It could be fine but I have found that when my plates get that diamond pattern, they never fruit. Same for the chalky pattern at 2:00.
Here is what I mean by diamond pattern:

Maybe diamond pattern isn't the best way to describe it but compare that to this (which is a textbook, clean pan mycelium pattern IMHO):
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mistamonsta
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27591264 - 12/22/21 09:53 PM (2 years, 1 month ago) |
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Thanks heaps Hindsight, thats a great explanation and pics to demonstrate 
It is very subtle but I can see by your two pics the difference in mycelium quality. As to those chalky/scabby looking colonies, they had me thinking bacteria or a mould that I hadn't seen before. This is exactly the info I needed for now 
I'll post back with results from my transfers in a few days
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: mistamonsta] 3
#27591387 - 12/23/21 02:26 AM (2 years, 1 month ago) |
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Hereโs some more pics I pulled from camera
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Drboomer
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27591462 - 12/23/21 05:28 AM (2 years, 1 month ago) |
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The humidity chamber likely won't have enough fae. It left my cubes with fuzzy feet. You'll have to drill holes like a mono to get it.
A sgfc and burying the containers in coir instead of perilite could work. I'm ripping off babas mono and lining it with coir to bury my pan bottles in when I try it. I'll post results.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Drboomer]
#27591486 - 12/23/21 06:14 AM (2 years, 1 month ago) |
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JOC what variety are those short chunky ones?
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Moseph
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27591696 - 12/23/21 10:10 AM (2 years, 1 month ago) |
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Alright. The first few are Cyanesces.



The next few are bisporus.



The temp finally dropped down here so I think they might've stalled. They should get a little more active if I warm them back up right?
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