|
EnDelfisE
Rider On The Storm


Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
Last seen: 8 hours, 18 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27588232 - 12/20/21 12:07 PM (2 years, 1 month ago) |
|
|
Guys any idea why this happened

I really though I has some nice sectors from this plate :
|
Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
|
Re: Official Copelandia/Panaeolus Thread [Re: EnDelfisE] 1
#27588247 - 12/20/21 12:20 PM (2 years, 1 month ago) |
|
|
I don't necessarily see anything wrong in that plate. It looks to me like your mycelium has eaten through the food coloring or dye you put in your agar. Sometimes they bleach it out completely and other times they eat certain components of the dye but leaving others in place which causes the dye to shift in color. The mycelium does look a bit funky though - but not necessarily. What agar formula are you using and what water source?
|
Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27588269 - 12/20/21 12:36 PM (2 years, 1 month ago) |
|
|

how may transfers from germ plate is this? might need a couple more.
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 2 hours, 19 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#27588279 - 12/20/21 12:48 PM (2 years, 1 month ago) |
|
|
To me it looks like the myc is struggling. The food coloring may be inhibiting it. Try to do a transfer to some normal agar without additives and go from there.
|
EnDelfisE
Rider On The Storm


Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
Last seen: 8 hours, 18 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27588355 - 12/20/21 01:49 PM (2 years, 1 month ago) |
|
|
Quote:
Hindsight said: I don't necessarily see anything wrong in that plate. It looks to me like your mycelium has eaten through the food coloring or dye you put in your agar. Sometimes they bleach it out completely and other times they eat certain components of the dye but leaving others in place which causes the dye to shift in color. The mycelium does look a bit funky though - but not necessarily. What agar formula are you using and what water source?
I mean it has just stuck there in the middle and doesn't want to grow outwards this happens to a second plate too, I have only had that happen with the pans my cubes grow as Normal The agar was, barley - oat - brown rice and tap water, 1 part gw to 4 parts water The lc I made from a transfer from that plate it grew fast and its thick, I don't understand why the mycelium doesn't want to spread!
|
EnDelfisE
Rider On The Storm


Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
Last seen: 8 hours, 18 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#27588356 - 12/20/21 01:50 PM (2 years, 1 month ago) |
|
|
Quote:
Adas said: To me it looks like the myc is struggling. The food coloring may be inhibiting it. Try to do a transfer to some normal agar without additives and go from there.
Hmmm I think maybe you that's a good idea! Thanks! Damn food coloring!
|
Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
|
Re: Official Copelandia/Panaeolus Thread [Re: EnDelfisE]
#27588358 - 12/20/21 01:54 PM (2 years, 1 month ago) |
|
|
Yes I second the advice on food coloring - just skip it. I too use GWA and have great results on it. I'm at about the same ratio as you - somewhere around 1/4 to 1/3 grain water and the rest regular water. I don't use tap water though - I use reverse osmosis or filtered water. I take it you are using the standard amount of agar (10g to 500ml)?
|
EnDelfisE
Rider On The Storm


Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
Last seen: 8 hours, 18 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27588422 - 12/20/21 02:52 PM (2 years, 1 month ago) |
|
|
Quote:
Hindsight said: Yes I second the advice on food coloring - just skip it. I too use GWA and have great results on it. I'm at about the same ratio as you - somewhere around 1/4 to 1/3 grain water and the rest regular water. I don't use tap water though - I use reverse osmosis or filtered water. I take it you are using the standard amount of agar (10g to 500ml)?
Yes I think there is just something in there that confuses pans, actually now I remember that this time I used the water that my dehydrator picked up and I also added the brown rice water plus the coloring, normally I just use the barley-oat water since I use that for Spawn, you are right 500 ml to 10 g agar, I'm going to make some simple agar and update you )
|
EnDelfisE
Rider On The Storm


Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
Last seen: 8 hours, 18 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27588427 - 12/20/21 02:55 PM (2 years, 1 month ago) |
|
|
Quote:
Baba Yaga said:

how may transfers from germ plate is this? might need a couple more.
This was the first transfer )
|
Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
|
Re: Official Copelandia/Panaeolus Thread [Re: EnDelfisE]
#27588438 - 12/20/21 03:01 PM (2 years, 1 month ago) |
|
|
When you say dehydrator, do you mean dehumidifier? If so, I wouldn't use the water that comes out of that. It's going to be loaded with molds and bacteria. Granted, it should be killed during PC cycle but still.... better safe than sorry.
|
Mycoplex
Sporocarp


Registered: 10/09/21
Posts: 818
Last seen: 5 hours, 19 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27588444 - 12/20/21 03:06 PM (2 years, 1 month ago) |
|
|
@EnDelfisE One thing you may want to check if whether or not the food dye you used (the specific color or combination of colors that you used) contains Red #3. This specific red food coloring inhibits fungal growth, another shroomery member (D3_Myc) showed this with an experiment: The Potential Negative Effects of Erythrosine (Red#3) on Mycelium. A Comparison of Growth on Assorted Dyed Agar Mediums
You can cut out the food coloring entirely like others said, but if you happen to notice that you are using one that contains Red#3, just know that could be a factor specifically.
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 2 hours, 19 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27588499 - 12/20/21 04:02 PM (2 years, 1 month ago) |
|
|
Quote:
Hindsight said: When you say dehydrator, do you mean dehumidifier? If so, I wouldn't use the water that comes out of that. It's going to be loaded with molds and bacteria. Granted, it should be killed during PC cycle but still.... better safe than sorry.
I don't see how that would be an issue. Grains that we sterilize are full of spores and endospores and it works.
|
Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
|
Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27588523 - 12/20/21 04:13 PM (2 years, 1 month ago) |
|
|
I hear you but I subscribe to the "don't push your luck club". I see evidence all the time that sterilization may not be as black and white as we sometimes think.
Here is an example from p9hu7: https://www.shroomery.org/forums/showflat.php/Number/27587577#27587577
He claims if he doesn't rinse his grains really well before he PC's them, he will get bacterial contamination routinely. The black and white outlook on that is that it shouldn't matter because anything in there will be killed by a 90 minute PC.
It's really easy to use clean or distilled water so my opinion is, why not use it and be on the safer side, vs using water that has fallen through a moldy/bacterial dehumidifier evaporator and came from the house air which is likely loaded with contaminates.
I do wish it were black and white though, and maybe it is and the kind of thing p9hu7 posted above is caused by something else and the correlation he is making is incorrect. Who knows.
|
Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27588740 - 12/20/21 07:47 PM (2 years, 1 month ago) |
|
|
Ditched all the tubs today cause they weren't going anywhere .... ....as my uncle always said: "you can't turn shit into chocolate".
 
Moving on and cooking up some grain tonight to re-run the clone I fucked up in a few more tubs.
|
EnDelfisE
Rider On The Storm


Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
Last seen: 8 hours, 18 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27588990 - 12/20/21 11:49 PM (2 years, 1 month ago) |
|
|
Quote:
Hindsight said: When you say dehydrator, do you mean dehumidifier? If so, I wouldn't use the water that comes out of that. It's going to be loaded with molds and bacteria. Granted, it should be killed during PC cycle but still.... better safe than sorry.
Agh yes I'm sorry my brain failed me :P I used the dihumidifier water, I thought because its fairly new and it has a function that activates UV light sterilization inside the machine that the water should better than tap since its just pure water from the air, but maybe you are right , if I put some of that water on agar do you think I can see how contaminated it is? but actually, possibly what I do is overkill.. Studies have shown the quality of tap water in Greece is supposed to be one the very best in Europe
Quote:
Mycoplex said: @EnDelfisE One thing you may want to check if whether or not the food dye you used (the specific color or combination of colors that you used) contains Red #3. This specific red food coloring inhibits fungal growth, another shroomery member (D3_Myc) showed this with an experiment: The Potential Negative Effects of Erythrosine (Red#3) on Mycelium. A Comparison of Growth on Assorted Dyed Agar Mediums
You can cut out the food coloring entirely like others said, but if you happen to notice that you are using one that contains Red#3, just know that could be a factor specifically.
Agh thank you for this info I've run out bit ill make sure to check at the supermarket next time!
Edited by EnDelfisE (12/20/21 11:55 PM)
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 2 hours, 19 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#27589056 - 12/21/21 03:00 AM (2 years, 1 month ago) |
|
|
I haven't personally had endogenous bacterial contam since I've started to use a pressure cooker some 3 years ago. I'm using Wheat but I'm not sure that matters.
Quote:
Baba Yaga said: Ditched all the tubs today cause they weren't going anywhere .... ....as my uncle always said: "you can't turn shit into chocolate".
 
Moving on and cooking up some grain tonight to re-run the clone I fucked up in a few more tubs. 
Wow.. Did they just stop growing like that?
|
Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
|
Re: Official Copelandia/Panaeolus Thread [Re: Adas] 3
#27589066 - 12/21/21 03:33 AM (2 years, 1 month ago) |
|
|
They were slow, the subs basically pinned 360o. Too many pins at the same time I guess. Will spawn new clones to 6 tubs on Thursday morning before I leave.
Edited by Baba Yaga (12/21/21 03:44 AM)
|
cambocyan

Registered: 06/24/20
Posts: 74
|
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 4
#27589116 - 12/21/21 06:09 AM (2 years, 1 month ago) |
|
|
copelandia Bunnel Florida Thanks to Adas for sharing this genetic.
--------------------
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 2 hours, 19 minutes
|
Re: Official Copelandia/Panaeolus Thread [Re: cambocyan] 1
#27589121 - 12/21/21 06:19 AM (2 years, 1 month ago) |
|
|
Hell yea buddy! I love the ragged caps on them, I've noticed most available Pan varieties don't have that. They're really special. Glad you enjoy!
|
Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 5 days
|
Re: Official Copelandia/Panaeolus Thread [Re: Adas] 3
#27589207 - 12/21/21 08:02 AM (2 years, 1 month ago) |
|
|
Mediocre first flush of a clone culture. Bigger fruits than last time I think, but not nearly as full of a flush.
|
|