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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27584991 - 12/17/21 02:37 PM (2 years, 1 month ago)

Thanks Adas and JOC.

Hey JOC one question for you back to the previous topic of substrate - do you find seeds in your straw and if so, do you find it's ok to use that straw so long as it's pasteurized? I mentioned it a couple pages back - the seeds in the straw are very easy to miss when dry but after pasteurization hydrates them, they look a lot like oats and I worry they are a vector for contams like mold or pathogenic bacteria.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27585080 - 12/17/21 03:56 PM (2 years, 1 month ago)

I don’t have seeds in mine so I honestly can answer whether it would hurt u or not


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] * 2
    #27585267 - 12/17/21 06:23 PM (2 years, 1 month ago)

So I really had to slow down here and take a breather before and remind me to focus on what I'm doing when I'm doing it and to actually
look at the things that are in front of my eyes.

I posted earlier that my currently running clone tubs are developing a lot of side pins again and I think I know now why that happens and
I hope I can avoid this with the next run of tubs although I am hard pressed to get the tubs set up before I leave for the Christmas
family meeting.......I might have to run a few more tubs if timing doesn't work out..........Hurry up you jars!

Ok, now that I got that out of the system I will elaborate on what I think is the culprit here.


With the last tubs I misted too much too early, I remember now that I saw this light white fluff on top of the substrates and I knocked
it completely back with a hard aggressive mist as I thought it would help to prevent overlay and left a really wet surface behind in the
process. I'm sure I destroyed the fruiting surface and condition by doing so.

Pans are moving fast and IMO there is only a narrow time window to get it right and now I'm left with happy side pins and almost
nothing on top of the substrate.




Supporting this theory is what I can see on the smaller coir only tubs. With them I wasn't so aggressive at the beginning and only
applied a light mist and kind of forgot them for a day.

What I can see is that surface pinning happens where this light fluff pokes through.




After noticing this I left the lid off of one of the tubs with extreme side pins to let the surface dry up so the mycelium has a
easier time to recover and it helped but I guess it's too late for those tubs to develop a good pin set.




I'm pretty confident that this is what caused the extreme side pinning as it happened to 3 different clones in 4 tubs which I
all treated the same and two of these clones performed well in a couple of previous tubs which I treated more "gently" a the beginning.

This shows how important it is to keep focused, I almost forgot that I did mist differently with these tubs cause I was on autopilot.
Have to keep this in mind for the future.


Edited by Baba Yaga (12/17/21 10:16 PM)


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #27585270 - 12/17/21 06:23 PM (2 years, 1 month ago)

Update on the C1T5 clone which was fruited @ spawn, h-manure/coir @ 50/50,spawn ratio 1:3, 1.5-2" main substrate + 0.5" top layer,
cased and increased FAE after 6 days at full colonization, first pin appeared 1 day after casing.

Only minimal amount of side pins, maybe 30-40 or so.




Cased at full colonization while having a double layer of micropore tape on the bottom holes. FAE increased straight after casing by
removing the micropore tape on the bottom holes.

As mentioned in a previous post, knots where forming on the interface of main substrate and the top layer and above.




Not many new knots formed directly on the surface, instead pins emerged from deeper down.




Resulting pins are fewer and not so much condensed into clusters but the pins are roughly twice as big/fat as when fruited
after a consolidation period.




It seems with this approach of fruiting that the substrate starts earlier with knot formation when exposed to FAE during colonization.
Kind of like the mycelium already gets an idea of where the action needs to start while colonizing and consolidating before fruiting and
then blasting FAE seems to be more like "oh wow it's happening guys hurry up we need to get this show on the road...go...GO!..GO!..GO!

Day 3 and day 4 of fruiting



Edited by Baba Yaga (12/17/21 10:23 PM)


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #27585271 - 12/17/21 06:24 PM (2 years, 1 month ago)

Update on the 100% coir tubs:

Clone C2T4, 100% coir, spawn ratio 1:2, fully colonized after 6 days, cased straight after full colonization, photos @ 4 days in fruiting conditions




Clone C1T5, 100% coir, spawn ratio 1:2, fully colonized after 7 days, cased straight after full colonization, photos @ 4 days in fruiting conditions




Clone C5T4, 100% coir, spawn ratio 1:2, fully colonized after 7 days, cased straight after full colonization, photos @ 4 days in fruiting conditions



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InvisibleMr Piggy
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27585306 - 12/17/21 06:50 PM (2 years, 1 month ago)

Baba I wish I could +5 you again.  Your highly detailed posts that often include the steps you took are incredibly informative.  Thank you.


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OfflineEnDelfisE
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27585456 - 12/17/21 08:33 PM (2 years, 1 month ago)

Quote:

Hindsight said:
You sure that's foam and not mycelium? Foam would be bad. Mycelium would be good. I use a magnetic stir bar to stir the culture daily and prevent growth on the surface. That surface growth really just gets in the way of things for me.




Oh yes,  I'm sorry my bad, I was meaning to say mycelium foam

I think it'd time to get a magnet stirrer,  thank you :smile:)


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27585695 - 12/18/21 01:40 AM (2 years, 1 month ago)

Quote:

It seems with this approach of fruiting that the substrate starts earlier with knot formation when exposed to FAE during colonization.




It's what I've noticed with Bisporus too! They seem to form legit knots in some places while colonizing. But then no matter what I do, I can't get them to pin. Knots form, but they never progress to pins, which is very strange.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27586259 - 12/18/21 01:54 PM (2 years, 1 month ago)

You are colonizing them while applying some FAE? What do you change after full colonization/consolidation to kick off fruiting?


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27586332 - 12/18/21 03:20 PM (2 years, 1 month ago)

Here’s what germinated from an ancient syringe I had laying around (guessing 3+ years old).



Is this what early pan cambo myc looks like or am I growing mold?

This is the general vicinity I put the drop  so the location is ok. Also this particular agar recipe is shit so there’s that too…

It’s hard to tell in the pic, but the discoloration looks to be due to the myc consuming it, not sporulation or something else.


Edited by Scrimshaw (12/18/21 03:22 PM)


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Invisiblerazero
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Re: Official Copelandia/Panaeolus Thread [Re: Scrimshaw] * 1
    #27586346 - 12/18/21 03:38 PM (2 years, 1 month ago)

Another set of transfers and I am ready for pans :mushroom2:



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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27586348 - 12/18/21 03:41 PM (2 years, 1 month ago)

Quote:

Baba Yaga said:
You are colonizing them while applying some FAE? What do you change after full colonization/consolidation to kick off fruiting?




Yes, I have a plastic sheet over the flowerpot, but it's bent so there's plenty FAE too. With the smaller tub I am trying to fruit now, I've just cased it and misted sometimes, but the misting seems to have damaged any forming promordia. With Cyans, the primordia are not hurt by misting. I'm kinda clueless.


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OfflineMycoplex
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Re: Official Copelandia/Panaeolus Thread [Re: razero]
    #27586356 - 12/18/21 03:49 PM (2 years, 1 month ago)

That agar looks good razero.  Good luck!

In another thread I wrote about difficulties I had with fruiting pans using the MacMerdin Simple Pan Cakes tek.  I was about to give up on these little containers today after 7 days without any sign of pins, but I noticed some tiny pins popping out.

Admittedly I am not very proficient with pans so I know I screwed something up here, including probably over-watering these, not giving them enough FAE, probably fruiting them too early, and a combination of all of the above.  I see there is overlay on the casing layer too which is another bad sign.

I live in a climate that makes fruiting from cakes really difficult which is why I almost always spawn cakes to bulk.  Using a SGFC is not practical for me because of how dry the ambient air is even when accounting for manual humidification.  I think most people see pins with this tek in 3 days or so but it took me over twice that long.



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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Scrimshaw]
    #27586359 - 12/18/21 03:51 PM (2 years, 1 month ago)

Quote:

Scrimshaw said:
Here’s what germinated from an ancient syringe I had laying around (guessing 3+ years old).



Is this what early pan cambo myc looks like or am I growing mold?

This is the general vicinity I put the drop  so the location is ok. Also this particular agar recipe is shit so there’s that too…

It’s hard to tell in the pic, but the discoloration looks to be due to the myc consuming it, not sporulation or something else.





If you would show me these plates without context then I'd say this is mold.

The discoloration is looking suspect and the myc has this grainy powdery look from what I can see in the pics.

Hold it up to a lamp and see what this looks like, molds like trich blocking out light more than mushroom mycelium IME.

But either way, it will show itself soom, if this hasen't turned green in three days then post some more photos and maybe it's good enough to
take some transfers.



@razero: :thumbup:


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InvisibleScrimshaw
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27586363 - 12/18/21 03:54 PM (2 years, 1 month ago)

Thanks Baba Yaga. My gut tells me it’s mold too but I’ll let it ride out for a few more days and try the light idea too.


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Invisiblerazero
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Re: Official Copelandia/Panaeolus Thread [Re: Scrimshaw]
    #27586365 - 12/18/21 03:56 PM (2 years, 1 month ago)

Myco, Baba - Thanks :thumbup:

Scrimshaw - looks like you have mold spores on your plate, should turn green/grey in a few days.

edit: mold not dust


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Edited by razero (12/18/21 04:13 PM)


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InvisibleScrimshaw
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Re: Official Copelandia/Panaeolus Thread [Re: razero]
    #27586366 - 12/18/21 03:58 PM (2 years, 1 month ago)

Quote:

razero said:

Scrimshaw - looks like you have dust spores on your plate, should turn green/grey in a few days.




There is definitely dust all over the lid, but none on the plate itself. It’s so hard to take quality pics of a plate sometimes.


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Invisiblerazero
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Re: Official Copelandia/Panaeolus Thread [Re: Scrimshaw]
    #27586372 - 12/18/21 04:02 PM (2 years, 1 month ago)

Quote:

Scrimshaw said:
Quote:

razero said:

Scrimshaw - looks like you have dust spores on your plate, should turn green/grey in a few days.




There is definitely dust all over the lid, but none on the plate itself. It’s so hard to take quality pics of a plate sometimes.



I meant mold spores not dust, my bad. I had the same thing happen to me not long ago.


Edited by razero (12/18/21 04:19 PM)


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27586377 - 12/18/21 04:10 PM (2 years, 1 month ago)

Quote:

Adas said:
Quote:

Baba Yaga said:
You are colonizing them while applying some FAE? What do you change after full colonization/consolidation to kick off fruiting?




Yes, I have a plastic sheet over the flowerpot, but it's bent so there's plenty FAE too. With the smaller tub I am trying to fruit now, I've just cased it and misted sometimes, but the misting seems to have damaged any forming promordia. With Cyans, the primordia are not hurt by misting. I'm kinda clueless.





Ok, hmmm I thought they were more forgiving. I would try upping consolidation time and mist up until primordia are showing up, then restrict FAE a bit and bottom water or use a syringe.

Hard to say without being able to stick my nose in that tub.

Not many grow logs around I guess.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Mycoplex] * 1
    #27586388 - 12/18/21 04:19 PM (2 years, 1 month ago)

Quote:

Mycoplex said:
That agar looks good razero.  Good luck!

In another thread I wrote about difficulties I had with fruiting pans using the MacMerdin Simple Pan Cakes tek.  I was about to give up on these little containers today after 7 days without any sign of pins, but I noticed some tiny pins popping out.

Admittedly I am not very proficient with pans so I know I screwed something up here, including probably over-watering these, not giving them enough FAE, probably fruiting them too early, and a combination of all of the above.  I see there is overlay on the casing layer too which is another bad sign.

I live in a climate that makes fruiting from cakes really difficult which is why I almost always spawn cakes to bulk.  Using a SGFC is not practical for me because of how dry the ambient air is even when accounting for manual humidification.  I think most people see pins with this tek in 3 days or so but it took me over twice that long.







I wouldn't call this overlay yet.

From what I hear dry climate can be a real bummer. IDK never did much pan cakes or fruited them in a humidity chamber.

Just shooting from the hip, but I would try a couple of cakes and recase them once primordia are showing, to cover them up with
something light and fluffy to keep a microclimate till they are a bit bigger, not sure about how moist to keep this additional casing, would
also try different materials as well like coir or verm or a mix or stick with 50/50. That's a matter of trial and error.


A.k.a is a desert dweller IIRC maybe he can share some experience.


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