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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #27576082 - 12/10/21 12:28 PM (2 years, 1 month ago)

Quote:

Baba Yaga said:
The first couple of clone tubs are moving fast. Colonized in 5 days at my usual spawn ratio of 1:2.5.
I think they are ready to be cased as they are pushing out metabolites already.



Got more tubs following so I might just give it a go.





Casing after 5 days wasn't enough...got lots of knotting on the sides and no pins after 3 days of fruiting which is unusual for me,
especially with the current temps of 76-80oF.

This is what happens when you go against your own advice :lol:

The other two tubs following (same clones) are at the 5 day mark now and fully colonized as well, will leave those to consolidate for a few
more days.

Those clones just got me too excited  :omgawesome:


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 7
    #27576931 - 12/11/21 05:56 AM (2 years, 1 month ago)

🍄❤️


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
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InvisibleDrboomer
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27577011 - 12/11/21 07:49 AM (2 years, 1 month ago)

Took a poke biopsy of pan cyan estero to lc wish me luck. Gonna drop the plate to grain this evening. Incase the lc dosnt turn out I should atleast be able to spawn something soon.

Would it be to hard to break up sub and grain mixed in a jar in an all in one Tek like asura uses? I'm out of bags. Maybe I'll just cook up a batch in half pint widemouths so I can just plop em out and break up by hand.


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OfflineElric
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Re: Official Copelandia/Panaeolus Thread [Re: Drboomer] * 6
    #27577050 - 12/11/21 08:33 AM (2 years, 1 month ago)

Popping off again and some squatty clusters 🍄





--------------------
~Elric~



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OfflineElric
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27577051 - 12/11/21 08:33 AM (2 years, 1 month ago)

Beautiful🍄🍄❤️


--------------------
~Elric~



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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Elric]
    #27577212 - 12/11/21 11:18 AM (2 years, 1 month ago)

@Monoid, I figured you would take it to agar first, just checking. I saw that you tried agar first, I just didn't want you to assume the LC is clean based on looks alone.

@Baba, I think trying the early casing was worth the experiment. Testing is the only way I learn what I'm doing right vs. what I'm doing wrong.

@Jake, Nice! You know someone like me will ask what variety those are unless you include that in the post. :smile: I'm not experienced enough to be able to tell by looks.

@DrBoomer, Good luck with your LC. I'm not experienced enough to say regarding sub and grain in a jar. Your idea of using WM half pints can definitely work though. I have only tried one pan LC, but it never grew. I should try it again...

@Elric, that's another beautiful flush coming in! Congrats!

Edit (Feb. 4th, 2022): Please see updated information towards bottom of post.

I may have found a solution to my cycle timer issue I've vented about before. It figures, it was something I already had on hand, so I could have saved about $50 by not buying the 2 timers. [Update: I could have saved about $25 by not buying 1 timer.] (The 2 timers were a few seconds off from each other, so one would catch up with the other within 24 hours, which made me have to reset one of them roughly every 12 hours.)

I had an old-school 10 minute cycle timer. It can operate as a dual throw switch, so it is operating both my fogger/fan AND my heater/fan. I can now see where the cycle is at a glance, AND it is now easy to manually skip it forward to either fog or heat. When I was using the 2 timers, it took too long to skip because I would have to wait for the correct time to start the other timer.

(The outlet has the connecting tabs cut, so the top is normally open, while the bottom is normally closed. An outlet would not work this way without that minor modification.)



My ideal schedule used to be 5 minutes of fog; 2 1/2 minutes of still air (nothing was on); 7 1/2 minutes of heat/fan; then the fog started again. (This was also dictated by the cycle timers, as the overall duration needed to be the same or as close as possible. I originally tried for 13 minutes instead of 15, but it was impossible.)

I'm still dialing in the new intervals, but I'm currently at 3 minutes 10 seconds of fog, and 6 minutes 50 seconds of heat/fan. (The temp controller runs heat until it reaches the set temperature, then only the fan runs for the rest of the duration.) [Update: This was too much airflow.]

I don't have anything in fruiting right now, so I will continue to dial in the intervals once I have trays in it. I used to use the 2 1/2 minutes of still air to allow the fog to settle on the trays/fruits. I won't be able to have any duration of still air, but I think it will be ok. [Update: It was not ideal.] As long as it works, this will make my life a bit easier not having to re-sync every morning and night.

Now I need to add a reservoir to maintain the water level in my fogger, and my setup could go for a few days without any maintenance, other than harvesting.

[Update: I eliminated my temperature controller because it beeped every ten minutes when the power came back on, and it was getting really annoying. I used one of the infinite cycle timers in place of the temp controller, so the fan no longer runs after the heat cycle. The heat and fan are once again forced to start and stop together, so I didn't have to rewire anything on the heater.

The heater and fan run for the amount of time determined by the infinite cycle timer, then shuts off. This allows for some still air, and the fruits are happier for this.

I also split up my alternating cycle timer into 2 normally open cycles and 2 normally closed cycles every 10 minutes. This means the fogger runs twice every 10 minutes, as does the heater.

My new cycle schedule looks like this: Fogger runs for 1 minute 38 seconds. Heater and fan come on as soon as the fogger turns off, and runs for 2 minute 32 seconds. The infinite cycle timer turns off the heat and fan, and everything is off for around 50 seconds. The fogger starts and the cycle repeats. This is where I was at when the room was cold. Once warmer temperatures return, this schedule will change a lot.

I have also raised the temperature of the water in the fogger reservoir to help warm the fog cycle air temperature. I have my aquarium heater running pretty hard, keeping the water around 90 to 91 degrees F. This is dependent on the room temperature, and is not needed to be so high when the room is warmer.

I have been meaning to edit this post for a while, but I also wanted to test the changes first. Now that I had some decent results using those parameters, and I have shut down for the winter, I had the time to update.]


Edited by ruawakeyet (02/04/22 02:43 PM)


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 2
    #27577223 - 12/11/21 11:30 AM (2 years, 1 month ago)

@ Drboomer - angam in our thread here uses jars in the way you are asking about. Check out the links in his signature for descriptions of how he manages it.


--------------------
What are they saying? Listen carefully, it might be something you need to hear...





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InvisibleBaba Yaga
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Registered: 09/13/20
Posts: 3,958
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #27577344 - 12/11/21 01:14 PM (2 years, 1 month ago)

Quote:

ruawakeyet said:
@Baba, I think trying the early casing was worth the experiment. Testing is the only way I learn what I'm doing right vs. what I'm doing wrong.






So true, testing things is what keeps this hobby interesting for me and now that I have the clones going it is even more fun as results
are not as vague as running MS.

But my pan growing season comes to an end soon, a couple more month minus a 2 week holiday. So I won't be doing much testing before the
end but running the 6 clones with my usual method will be a test in itself anyway. Other than that I will be taking prints for next time. 
Grow, clone, print, repeat.


Now on the morning of day 4 the pin set decided to come in.



Could have done with a bit less surface myc but the main thing is that they get to a reasonable size cause all the clones were taken from
pins, no idea what this is going to be like. :popcorn:


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27577370 - 12/11/21 01:35 PM (2 years, 1 month ago)

Impressive pinset! There are knots everywhere, I can't wait to see this one!


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Invisibleelpico
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Posts: 315
Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 3
    #27577666 - 12/11/21 04:43 PM (2 years, 1 month ago)

Some nice pinsets happenin' on this page.  I've got test jars of P. bisporus starting to show some love.  I've been wanting to grow this species for a long time, so I'm very pleased.



Thanks to the member here who hooked me up!


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Invisibleruawakeyet
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Posts: 1,862
Re: Official Copelandia/Panaeolus Thread [Re: elpico]
    #27577863 - 12/11/21 07:29 PM (2 years, 1 month ago)

Nice Bisporus. I have wanted to grow them ever since I started reading this thread. I will someday.

Your signature inspires me to do more. :smile:


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #27577866 - 12/11/21 07:36 PM (2 years, 1 month ago)

Quote:

ruawakeyet said:
@Monoid, I figured you would take it to agar first, just checking. I saw that you tried agar first, I just didn't want you to assume the LC is clean based on looks alone.

@Baba, I think trying the early casing was worth the experiment. Testing is the only way I learn what I'm doing right vs. what I'm doing wrong.

@Jake, Nice! You know someone like me will ask what variety those are unless you include that in the post. :smile: I'm not experienced enough to be able to tell by looks.

@DrBoomer, Good luck with your LC. I'm not experienced enough to say regarding sub and grain in a jar. Your idea of using WM half pints can definitely work though. I have only tried one pan LC, but it never grew. I should try it again...

@Elric, that's another beautiful flush coming in! Congrats!


I may have found a solution to my cycle timer issue I've vented about before. It figures, it was something I already had on hand, so I could have saved about $50 by not buying the 2 timers. (The 2 timers were a few seconds off from each other, so one would catch up with the other within 24 hours, which made me have to reset one of them roughly every 12 hours.)

I had an old-school 10 minute cycle timer. It can operate as a dual throw switch, so it is operating both my fogger/fan AND my heater/fan. I can now see where the cycle is at a glance, AND it is now easy to manually skip it forward to either fog or heat. When I was using the 2 timers, it took too long to skip because I would have to wait for the correct time to start the other timer.

(The outlet has the connecting tabs cut, so the top is normally open, while the bottom is normally closed. An outlet would not work this way without that minor modification.)



My ideal schedule used to be 5 minutes of fog; 2 1/2 minutes of still air (nothing was on); 7 1/2 minutes of heat/fan; then the fog started again. (This was also dictated by the cycle timers, as the overall duration needed to be the same or as close as possible. I originally tried for 13 minutes instead of 15, but it was impossible.)

I'm still dialing in the new intervals, but I'm currently at 3 minutes 10 seconds of fog, and 6 minutes 50 seconds of heat/fan. (The temp controller runs heat until it reaches the set temperature, then only the fan runs for the rest of the duration.)

I don't have anything in fruiting right now, so I will continue to dial in the intervals once I have trays in it. I used to use the 2 1/2 minutes of still air to allow the fog to settle on the trays/fruits. I won't be able to have any duration of still air, but I think it will be ok. As long as it works, this will make my life a bit easier not having to re-sync every morning and night.

Now I need to add a reservoir to maintain the water level in my fogger, and my setup could go for a few days without any maintenance, other than harvesting.




Pan cyan Alabama (left)
Pan cam Jamaica (right)


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] * 1
    #27577885 - 12/11/21 07:45 PM (2 years, 1 month ago)

@ Babayaga … since you mentioned running with clones over time and the rest of you with experience relating to this question …

How do you maintain a prized pan culture over the long haul (meaning 4-6-12 months)?.

In my experience just keeping them on a plate doesn’t work very well because eventually it seems to go dormant or die after a couple of months. Slowing it down by popping it in the refrigerator doesn’t seem to be an option because I’ve noticed less than 40-45 degrees seems to kill my cultures.

Do you just keep transferring from one plate to the next every 4-6 weeks? Will a pan culture keep longer in a low nutrient density LC? 

I’m guessing you pan experts have a tried and true strategy fir keeping your best cultures going indefinitely…


--------------------
What are they saying? Listen carefully, it might be something you need to hear...





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Invisibleelpico
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] * 5
    #27577891 - 12/11/21 07:51 PM (2 years, 1 month ago)

Colonized agar plugs in distilled water at room temperature works great.  I plated out a 2-3 year old Pan cyan clone culture recently, and it was still vigorous.  I've switched over to this after sharing your experience of losing plates and slants in the fridge after less than six months.


Edited by elpico (12/11/21 07:55 PM)


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] * 1
    #27577922 - 12/11/21 08:05 PM (2 years, 1 month ago)

Quote:

PsiloPsychIn said:
@ Babayaga … since you mentioned running with clones over time and the rest of you with experience relating to this question …

How do you maintain a prized pan culture over the long haul (meaning 4-6-12 months)?.

In my experience just keeping them on a plate doesn’t work very well because eventually it seems to go dormant or die after a couple of months. Slowing it down by popping it in the refrigerator doesn’t seem to be an option because I’ve noticed less than 40-45 degrees seems to kill my cultures.

Do you just keep transferring from one plate to the next every 4-6 weeks? Will a pan culture keep longer in a low nutrient density LC? 

I’m guessing you pan experts have a tried and true strategy fir keeping your best cultures going indefinitely…





These are my first clones and I am working on stabilizing genetics that will do well in mono tubs if that is even possible. I only grow
for about 6 month a year so my goal is simply to grow out a print form a previous clone, clone again and take prints from that for the next
round.

I never had much luck with long term storage on plates, that is why I played around with dried grain spawn and now I have made quite a few
LCs for that purpose with a nutrient content of 0.1% LME. I also have some agar wedges in distilled water and will have to see what the
survival rate is with these two methods.

Maybe a PF type substrate will do better in the fridge.

Still have to figure this one out myself so thanks for asking will be interested in answers as well.


Edit: yeah great elpico, you inspired me to try the agar wedge in distilled water method :thumbup:


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: elpico]
    #27577926 - 12/11/21 08:07 PM (2 years, 1 month ago)

Psilo, I'm glad you asked. I asked the same about a month ago, because of the same issue on agar. I was up to a T4 and I let them sit on the plates longer than needed each time.

I'm going to try switching to G2G, both for inoculation and to keep the culture off of agar and on grain instead. I figure if I use a little spawn to start 1 or 2 grain jars, let those colonize, then use them to spawn the next round of grain jars, the timing should work out well. I'm thinking this will give me a good reason to get some rye berries and finally try them for the first time. I might alternate between that and my usual milo and millet.

Elpico, was that culture stored at room temperature? I like the distilled water idea a lot, and will be trying that as well. Thanks!


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #27578117 - 12/11/21 10:50 PM (2 years, 1 month ago)

Thank you elpico
From what I’ve heard sterilized water with next to zero nutrients is a good long term storage for fungal cultures in general. Stametes recommends an agar slant with gas exchange and cold (refrigerated) storage. He says that can work for up to five years, but suggests keeping multiple back ups and reslanting or or two of them once per year. He doesn’t mention pans but he does note that paddy straw mushroom mycelium cannot be chilled.


--------------------
What are they saying? Listen carefully, it might be something you need to hear...





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InvisibleDrboomer
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #27578235 - 12/12/21 04:03 AM (2 years, 1 month ago)

Thanks psilo that's just what I was looking for.


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: elpico]
    #27578247 - 12/12/21 04:34 AM (2 years, 1 month ago)

Quote:

elpico said:
Some nice pinsets happenin' on this page.  I've got test jars of P. bisporus starting to show some love.  I've been wanting to grow this species for a long time, so I'm very pleased.



Thanks to the member here who hooked me up!




Very cool! I wonder why they just never pin for me. It's been nearly a month in my setup and nothing happened. Only some white primordia-like things formed, and then nothing. Misting didn't help wither.


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Invisibleelpico
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 2
    #27578339 - 12/12/21 07:56 AM (2 years, 1 month ago)

Adas, based on your previous posts, I think we have the same set of genetics.  For me, these guys are trickier than pan cyans.  I have four MS jars, and they overlaid pretty badly.  I left them for weeks in the FC, and barely got a few anemic fruits in one jar.  So I scraped the casing off, dunked(bottom-water only), and kept the jars covered a few days until the surface mycelium reovered.  Re-cased and put back into my jcm-stlye FC.  They still wanted to overlay.  I misted very heavily morning and evening, and now all four jars are fruiting.  The pins are mainly around the edges where the overlay/drying was less of a problem.  I'm sure you'll nail it soon!


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