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Offlinenksfo5
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Registered: 12/19/12
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22323047 - 10/02/15 10:54 AM (8 years, 3 months ago)

Well my first attempt with pans has been a let down and they have subcumbed to a nasty field of tek

Better luck next time

Great thread FOX keep it up


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Official Copelandia/Panaeolus Thread [Re: nksfo5]
    #22323074 - 10/02/15 11:01 AM (8 years, 3 months ago)

Wot?!! Nuuu..

I'm sorry..
I wish you success in your next attempt.
Don't give up!

What do you mean by "nasty field tek"??


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Giving is all we have, for we're just grateful to be alive

:kratom:


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: Official Copelandia/Panaeolus Thread [Re: nksfo5]
    #22324957 - 10/02/15 05:59 PM (8 years, 3 months ago)

Quote:

nksfo5 said:
Well my first attempt with pans has been a let down and they have subcumbed to a nasty field of tek

Better luck next time

Great thread FOX keep it up



Did you pasteurize the poo properly? This seems to be where most people fail. That, and having clean spawn to work with.


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Invisibleeatyualive
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful] * 1
    #22328646 - 10/03/15 02:37 PM (8 years, 3 months ago)

I love unas old pan tek. Real easy. Soak straw overnight. Mix it with 50/50 verm coir and pc in spawn bag an hour. Spawn. Let colonize. Add casing. Pans fruit invitro really well also.

Una's Pan Cyan Tek



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Offlinenksfo5
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Registered: 12/19/12
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Re: Official Copelandia/Panaeolus Thread [Re: eatyualive]
    #22332502 - 10/04/15 12:33 PM (8 years, 3 months ago)

I meant a nasty field of trich

I pasteurized the horse shit but I only did a chemical pasteurization of the straw so that's where I messed up I forgot to throw my straw in jars it was just a misplaced memory for some reason or the other ill be giving it another go soon

The spawn was clean it was just a forgotten step that fucked me and fucked me good

I have no more pan cyan though so I'll be. Using my pan cam Goliath on my next go

This second run though I'm going to get a new tub and modify it specifically for pans and cut 6 1 inch holes at 2.25/2.5 inches for the bottom holes and 2 one  point five inch holes for the top holes and then I'll skip the straw and use 100 percent hpoo or I'll use a mix of 60/70 horse manure and 40/30 coir along with some gypsum and oyster shell thrown in


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InvisibleFantastic Mr. Fox
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Posts: 3,225
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Re: Official Copelandia/Panaeolus Thread [Re: nksfo5]
    #22332768 - 10/04/15 01:35 PM (8 years, 3 months ago)

:goodluck:

Panaeolus cyanescens ready for a 1/4" casing layer


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22332779 - 10/04/15 01:38 PM (8 years, 3 months ago)

Nicee!! Got my pan cyan spores on the way, gonna put a drop on agar using the "divot" method you mentioned before. :dancer:


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InvisibleFantastic Mr. Fox
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Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22332921 - 10/04/15 02:10 PM (8 years, 3 months ago)


Or you could sterilize a Q-tip, and place a drop of the spore solution on the swab, then swipe the dish.
That way you can avoid the divot all together..

Your call.

Either way :woot:


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Giving is all we have, for we're just grateful to be alive

:kratom:


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22333235 - 10/04/15 03:20 PM (8 years, 3 months ago)

Interesting..I might do that. I could PC a bunch of Q-tips while loading up some more agar dishes in there as well. Kills two birds with one stone :lol:

Do you think 30 minutes in the PC will be enough for both agar/Q-tips? I wanna try to keep my condensation down on this round of pasty's. And I'll be bringing the pressure down very slowly this time.


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OfflinetaGyo
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful] * 1
    #22333242 - 10/04/15 03:23 PM (8 years, 3 months ago)

I do 45 min with my agar and do swabs in a glass jar. 45 min is reg for agar so I just keep them in there for that. You're right, it's a great way to get away with more bang for your buck from a PC cycle.

This thread is awesome FF :rockon:


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Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Official Copelandia/Panaeolus Thread [Re: taGyo]
    #22333576 - 10/04/15 04:57 PM (8 years, 3 months ago)

:wave:

15mins is sufficient for swabs, and standard agar media.

Manure/straw/grain water agar needs 25-30min IME


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Giving is all we have, for we're just grateful to be alive

:kratom:


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Offlineaerow.thefox
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22333598 - 10/04/15 05:02 PM (8 years, 3 months ago)

yea every other pc load i do has a jar filled with qtips, syringes, or exacto blades.:lol:


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Official Copelandia/Panaeolus Thread [Re: aerow.thefox]
    #22333623 - 10/04/15 05:09 PM (8 years, 3 months ago)

Yupp,
My run consists of agar, swabs, & my lovely scalpel...
#3 handle w/#12 blade

Never jumped on the X-acto wagon..


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Giving is all we have, for we're just grateful to be alive

:kratom:


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22333642 - 10/04/15 05:13 PM (8 years, 3 months ago)

Quote:

taGyo said:
I do 45 min with my agar and do swabs in a glass jar. 45 min is reg for agar so I just keep them in there for that.



Yeah that's what Pasty recommends for his plates, I try to follow his tek to a T, I don't wanna mess up anything.

Quote:

Fantastic Mr. Fox said:
15mins is sufficient for swabs, and standard agar media.



Ive also heard 15 minutes was sufficient too, that's why I wanted to be safe and PC for 30 minutes, better to be in the middle. :shrug:


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InvisibleMad Season
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22333650 - 10/04/15 05:16 PM (8 years, 3 months ago)

Even 10 minutes at 15 psi will sterilize agar enough. I do max 20 minutes these days. I don't like caramelizing the media


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OfflinePsilosoulful

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Re: Official Copelandia/Panaeolus Thread [Re: Mad Season]
    #22333653 - 10/04/15 05:18 PM (8 years, 3 months ago)

:takingnotes:


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OfflinetaGyo
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22333697 - 10/04/15 05:28 PM (8 years, 3 months ago)

Guys.

Come on. When you know stuff like that you make a thread on the first page to save Gyo here 25 minutes an agar run :laugh2:, no harm no foul anyway doesn't bother me to leave the extra time.


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Official Copelandia/Panaeolus Thread [Re: taGyo]
    #22333934 - 10/04/15 06:15 PM (8 years, 3 months ago)

Noted. I guess my main issue with agar is not the sterilization time, but reducing the amount of condensation on the walls. I'm gonna start using glass dishes (half pint wide mouth) and reduce the pressure slowly after 20 minutes is reached. Hopefully they work this time.  :megaman:


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InvisibleMr.PhilCybin
Master Baiter
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Registered: 06/13/11
Posts: 11,642
Loc: Gnarnia
Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22334110 - 10/04/15 06:47 PM (8 years, 3 months ago)

to the 20 min-agar guys -

do you sterilize your plates on a previous cycle?  or would the assumption be that 20 min is more than enough time to sterilize glass?


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I'm stupid, Falcon91Wolvrn03 is smart.
I'm ugly, Falcon91Wolvrn03 is beautiful.
I'm a loser, Falcon91Wolvrn03 is a winner.
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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Official Copelandia/Panaeolus Thread [Re: Mr.PhilCybin]
    #22334969 - 10/04/15 10:00 PM (8 years, 3 months ago)

I would think sterilizing agar in a glass jar vs a plastic dish would take the same amount of time, not completely sure tho. :shrug:

I've seen a lot of people using the half pint wide mouth jars lately, that's what I'll be using (with plastic lids and 1/4" GE hole covered with a piece of SFD).

My PC time will be 20 minutes at 15 psi (along with the Q-tips), then reduce the heat SLOWLY. This should result in very minimal condensation on the sides of the glass jar. I'll keep my fingers crossed. :aum:


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